Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods

Xu, Feili,Wang, Junping,Guo, Yunchang,Fu, Ping,Zeng, Huawei,Li, Zhigang,Pei, Xiaoyan,Liu, Xiumei,Wang, Shuo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.

Active mass driver control system for suppressing wind-induced vibration of the Canton Tower

Xu, Huai-Bing,Zhang, Chun-Wei,Li, Hui,Tan, Ping,Ou, Jin-Ping,Zhou, Fu-Lin Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.13 No.2

In order to suppress the wind-induced vibrations of the Canton Tower, a pair of active mass driver (AMD) systems has been installed on the top of the main structure. The structural principal directions in which the bending modes of the structure are uncoupled are proposed and verified based on the orthogonal projection approach. For the vibration control design in the principal X direction, the simplified model of the structure is developed based on the finite element model and modified according to the field measurements under wind excitations. The AMD system driven by permanent magnet synchronous linear motors are adopted. The dynamical models of the AMD subsystems are determined according to the open-loop test results by using nonlinear least square fitting method. The continuous variable gain feedback (VGF) control strategy is adopted to make the AMD system adaptive to the variation in the intensity of wind excitations. Finally, the field tests of free vibration control are carried out. The field test results of AMD control show that the damping ratio of the first vibration mode increases up to 11 times of the original value without control.

Preparation and In Vivo Evaluation of Huperzine A-Loaded PLGA Microspheres

FU XU-DONG,GAO YONG-LIANG,PING QI-LENG,Ren Tang The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.9

Huperzine A-loaded microspheres composed of poly(D,L-lactide-co-glycolide) were prepared by an O/w emulsion solvent evaporation method. The characterization of the microspheres such as drug loading, size, shape and release profile was described. The in vitro release in the initial 7 days was nearly linear with $10\%$ released per day. Thereafter drug release rate became slow gradually and about $90\%$ drug released at day 21. The in vitro release rate determined by dialysis bag method had a good correlation with the in vivo release rate. Huperzine A aqueous solution was intramuscularly injected (i.m.) at 0.4mg/kg and microspheres were intra­muscularly injected at 8.4 mg eq huperzine A/kg in rats. The maxium plasma concentration $(C_{max})$ after i.m. microspheres was only $32\%$ of that after i.m. solution. Drug in plasma could be detectd until day 14 and about $5\%$ of administered dose was residued at the injection site at day 14. The relative bioavailability of huperzine A microspheres over a period of 14 days was $94.7\%$. Inhibition of acyecholinesterase activity (AchE) in rat's cortex, hippocampus and striatum could sustain for about 14 days. In conclusion, huperzine A-loaded microspheres possessed a prolonged and complete drug release with significant inhibition of AchE for 2 weeks in rats.

Preparation and In Vivo Evaluation of Huperzine A-Loaded PLGA Microspheres

XU-DONG FU,YONG-LIANG GAO,QI-NENG PING,Tang Ren 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.9

Huperzine A-loaded microspheres composed of poly(D,L-lactide-co-glycolide) were prepared by an O/W emulsion solvent evaporation method. The characterization of the microspheres such as drug loading, size, shape and release profile was described. The in vitro release in the initial 7 days was nearly linear with 10% released per day. Thereafter drug release rate became slow gradually and about 90% drug released at day 21. The in vitro release rate determined by dialysis bag method had a good correlation with the in vivo release rate. Huperzine A aqueous solution was intramuscularly injected (i.m.) at 0.4 mg/kg and microspheres were intramuscularly injected at 8.4 mg eq huperzine A/kg in rats. The maxium plasma concentration (Cmax ) after i.m. microspheres was only 32% of that after i.m. solution. Drug in plasma could be detectd until day 14 and about 8% of administered dose was residued at the injection site at day 14. The relative bioavailability of huperzine A microspheres over a period of 14 days was 94.7%. Inhibition of acyecholinesterase activity (AchE) in rat’s cortex, hippocampus and striatum could sustain for about 14 days. In conclusion, huperzine A-loaded microspheres possessed a prolonged and complete drug release with significant inhibition of AchE for 2 weeks in rats.

Sorafenib Continuation after First Disease Progression Could Reduce Disease Flares and Provide Survival Benefits in Patients with Hepatocellular Carcinoma: a Pilot Retrospective Study

Fu, Si-Rui,Zhang, Ying-Qiang,Li, Yong,Hu, Bao-Shan,He, Xu,Huang, Jian-Wen,Zhan, Mei-Xiao,Lu, Li-Gong,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Sorafenib is a promising drug for advanced hepatocellular carcinoma (HCC); however, treatment may be discontinued for multiple reasons, such as progressive disease, adverse events, or the cost of treatment. The consequences of sorafenib discontinuation and continuation are uncertain. Materials and Methods: We retrospectively analyzed 88 HCC patients treated with sorafenib from July 2007 to January 2013. Overall survival (OS), post-disease progression overall survival (pOS), and time to disease progression (TTP) were compared for survival analysis. Cox proportional hazard regression was performed to assess the effect of important factors on OS in the overall patient population and on pOS in patients who continued sorafenib treatment. Results: Sorafenib was discontinued and continued in 24 and 64 patients, respectively. The median OS (355 vs 517 days respectively; p=0.015) and median post-PD OS (260 vs 317 days, respectively; p=0.020) were statistically different between the discontinuation and continuation groups. Neither the median time to first PD nor the time to second PD were significantly different between the 2 groups. In the discontinuation group, 3 of the 24 patients (12.5%) suffered disease outbreaks. In Cox proportional hazard regression analysis after correction for confounding factors, BCLC stage (p=0.002) and PD site (p=0.024) were significantly correlated with pOS in patients who continued sorafenib treatment. Conclusions: Sorafenib discontinuation may cause HCC flares or outbreaks. It is advisable to continue sorafenib treatment after first PD, particularly in patients with Barcelona Clinic Liver Cancer stage B disease or only intrahepatic PD.

Experimental behavior of circular flyash-concrete-filled steel tubular stub columns

Si-Ping Li,Yang Zhang,Guang-Yuan Fu,Chen-Jiang Yu,Bing Chen,She-Xu Zhao 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.4

The paper presents an experimental study of the structural behavior of circular flyash-concrete-filled steel tubular stub columns under axial compressive loads. In this study, 90% and 100% by weight of the cement in the concrete core was replaced with flyash. Twenty-seven specimens were tested to study the influence of flyash content, wall thickness of the steel tube, and curing age on the ultimate capacity and confinement effect. The experimental results were compared with the design values calculated using AISC-LRFD (1999), ACI (1999), AIJ (1997) and Eurocode 4 (1994). From the experimental study, it was determined that the confinement effect of circular steel tubes filled with high content flyash concrete was better than that of specimens filled with ordinary Portland cement concrete. The 5.88-mm-thick steel tube filled with 100% flyash concrete was equivalent in strength to a steel tube filled with C30 concrete at 28 days.

Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods

Feili Xu,Junping Wang,Yunchang Guo,Huawei Zeng,Ping Fu,Zhigang Li,Xiaoyan Pei,Xiumei Liu,Shuo Wang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2

This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemicaltype strain also had similar antibiotic resistance patterns.

Active mass driver control system for suppressing wind-induced vibration of the Canton Tower

Huai-bing Xu,Jin-ping Ou,Chun-wei Zhang,Hui Li,Ping Tan,Fu-lin Zhou 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.13 No.2

In order to suppress the wind-induced vibrations of the Canton Tower, a pair of active mass river (AMD) systems has been installed on the top of the main structure. The structural principal directions n which the bending modes of the structure are uncoupled are proposed and verified based on the rthogonal projection approach. For the vibration control design in the principal X direction, the simplified odel of the structure is developed based on the finite element model and modified according to the field measurements under wind excitations. The AMD system driven by permanent magnet synchronous linear otors are adopted. The dynamical models of the AMD subsystems are determined according to the pen-loop test results by using nonlinear least square fitting method. The continuous variable gain feedback VGF) control strategy is adopted to make the AMD system adaptive to the variation in the intensity of wind excitations. Finally, the field tests of free vibration control are carried out. The field test results of AMD control show that the damping ratio of the first vibration mode increases up to 11 times of the original value without control.

Determining a Detectable Threshold of Signal Intensity in cDNA Microarray Based on Accumulated Distribution

( Xia Gao ),( Xu Ping Fu ),( Tao Li ),( Jian Zi ),( Yao Luo ),( Qing Wei ),( Er Liang Zeng ),( Yi Xie ),( Yao Li ),( Yu Min Mao ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.6

In microarray data mining, one of the key problems is how to handle weak signals. Based on a bent piecewise linear accumulated distribution generally found in the microarray data, a new detectable threshold finding method is proposed to filter genes with unreliable information in this paper. More reliable and reproducible data is produced for the subsequent data mining.

Electrospun PU/PVP/GO Separator for Li-ion Batteries

Kedong Song,Ping Zhang,Yuting Huang,Fu Xu,Yanhuai Ding 한국섬유공학회 2019 Fibers and polymers Vol.20 No.5

Polyurethane (PU) has been intensively studied as a promising separator material for Li-ion batteries (LIBs). However, poor dimensional stability and low liquid-electrolyte wettability of pure PU separator restrict its application inLIBs. Here polyvinyl pyrrolidone (PVP) and graphene oxide were employed to enhance the thermal stability, mechanicalperformance and liquid-electrolyte compatibility of PU separator. High anodic stability above 5.25 V was reached in PU/PVP/GO separator, fully compatible with the high-voltage cathode materials. The electrochemical characterization indicatesPU/PVP/GO membrane is a promising separator for advanced LIBs.