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( Sait Byul Park ),( In Ae Lee ),( Joo Won Suh ),( Jeong Gu Kim ),( Choong Hwan Lee ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.12
Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating pathogen to Oryza sativa and has been shown to cause bacterial blight. Two bioactive compounds showing antimicrobial activities against Xoo strain KACC 10331 were isolated from a Streptomyces bottropensis strain. The ethyl acetate extract was fractionated on a Sephadex LH-20 column, and then purified by preparative HPLC. The purified compounds were identified as bottromycin A2 and dunaimycin D3S by HR/MS and 1H NMR analyses. The MIC value against Xoo and the lowest concentration still capable of suppressing rice bacterial blight were 2 μg/ml and 16 μg/ml for bottromycin A2, and 64 μg/ml and 0.06 μg/ml for dunaimycin D3S, respectively. These two compounds were shown to exert different bioactivities in vitro and in rice leaf explants.
벼의 생엽절편을 이용한 병원균 억제물질의 대량 스크리닝 방법 개발
박샛별 ( Sait Byul Park ),이충환 ( Choong Hwan Lee ),김태종 ( Tae Jong Kim ),강린우 ( Lin Woo Kang ),이병무 ( Byoung Moo Lee ),김정구 ( Jeong Gu Kim ) 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 2012 한국미생물·생명공학회지 Vol.40 No.1
A new method for the high throughput screening of antagonistic substances against rice pathogens using rice leaf explants was developed. This method can be used to confirm the activities of any compound or mixture suppressing rice bacterial blight (BB) before field tests. Xanthomonas oryzae pv. oryzae (Xoo) culture medium was distributed in 96 well plates with equally sized explants and the active compounds were added to the wells. The strength suppressing BB was converted into an area percent of the lesion on the rice explants. The explants under BB suppressing activity remained uninfected maintaining their actual green color, while infected explants exhibited pale yellow-colored lesions. Based on the results, this method seems to be faster and easier, dose-dependent, and can be performed all-at-once with a small amount of unspecified compounds. This method also has the potential to be applied to inspection activities for the suppression of other waterborne crop diseases.
혈청 콜레스테롤과 식이 섭취와의 연관성에 대한 단면적 조사
박혜순,이규희,박샛별 한국지질학회 1997 韓國脂質學會誌 Vol.7 No.1
Background: Hyperlipidemia is a well recognized risk factor of coronary heart disease. In Korea, hypercholesterolemic patients are increasing now by westernized dietary pattern. Serum lipid level is known to be related to the dietary intake. The studies about the relationship of serum cholesterol and diet are not sufficient in Korea. We conducted this study to show the relationship of serum cholesterol and dietary intake among the Korean. Methods: Four hundred sixty nine patients who filled up a semi-quantitative food frequency questionnaire in the Lifelong Health Monitoring Program(LHMP) clinic were evaluated about serum cholesterol level. Dietary cholesterol amount, calories, carbohydrate, protein, fat content were calculated by the nutrition analysis program, and then Pearson correlation coefficients was used to evaluate the correlation between serum cholesterol and dietary contents. Results: Pearson correlation coefficients between serum cholesterol and dietary cholesterol, fat, calories are 0.03(P=0.6187), 0.05(P=0.3603), 0.08(P=0.1532) in men and -0.04(P=0.5873), -0.14(P=0.0611), -0.17(P=0.0215) in women. Conclusion: It seemed to be there is no correlation between serum cholesterol and dietary intake in both men and women. There is no need to restrict dietary cholesterol and fat in persons who have normal serum cholesterol level. We have to assess nutrient intake and manage by individualized approach for hypercholesterolemic patients.
Metabolite Profiling and Bioactivity of Rice Koji Fermented by Aspergillus Strains
( Ah Jin Kim ),( Jung Nam Choi ),( Ji Young Kim ),( Hyang Yeon Kim ),( Sait Byul Park ),( Soo Hwan Yeo ),( Ji Ho Choi ),( Kwang Hyeon Liu ),( Choong Hwan Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
In this study, the metabolite profiles of three Aspergillus strains during rice koji fermentation were compared. In the partial least squares discriminant analysis-based gas chromatography-mass spectrometry data sets, the metabolite patterns of A. oryzae (KCCM 60345) were clearly distinguished from A. kawachii (KCCM 60552) and only marginal differences were observed for A. oryzae (KCCM 60551) fermentation. In the 2 days fermentation samples, the overall metabolite levels of A. oryzae (KCCM 60345) were similar to the A. oryzae (KCCM 60551) levels and lower than the A. kawachii (KCCM 60552) levels. In addition, we identified discriminators that were mainly contributing tyrosinase inhibition (kojic acid) and antioxidant activities (pyranonigrin A) in A. oryzae (KCCM 60345) and A. kawachii (KCCM 60552) inoculated rice koji, respectively. In this study, we demonstrated that the optimal inoculant Aspergillus strains and fermentation time for functional rice koji could be determined through a metabolomics approach with bioactivity correlations.
GC-MS Based Metabolite Profiling of Rice <i>Koji</i> Fermentation by Various Fungi
KIM, Ah Jin,CHOI, Jung Nam,KIM, Jiyoung,PARK, Sait Byul,YEO, Soo Hwan,CHOI, Ji Ho,LEE, Choong Hwan Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, Biotechnology, and Biochemistry Vol.74 No.11
<P>In this study, <I>Aspergillus kawachii</I>, <I>Aspergillus oryzae</I>, and <I>Rhizopus</I> sp., were utilized for rice <I>Koji</I> fermentation, and the metabolites were analyzed in a time-dependent manner by gas chromatography-mass spectrometry. On Principal Component Analysis, the metabolite patterns were clearly distinguished based on the fungi species. This approach revealed that the quantities of glucose, galactose, and glycerol gradually increased as a function of fermentation time in all trials rice <I>Koji</I> fermentation. The time-dependent changes of these metabolites showed significant increases in glucose in the <I>A. oryzae</I>-treated rice, and in glycerol and galactose in the <I>A. kawachii</I>-treated rice. In addition, glycolysis-related enzyme activities were correlated with the changes in these metabolites. The results indicate that time-dependent metabolite production has the potential to be a valuable tool in selecting inoculant fungi and the optimal fermentation time for rice <I>koji</I>.</P>