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벼의 생엽절편을 이용한 병원균 억제물질의 대량 스크리닝 방법 개발
박샛별 ( Sait Byul Park ),이충환 ( Choong Hwan Lee ),김태종 ( Tae Jong Kim ),강린우 ( Lin Woo Kang ),이병무 ( Byoung Moo Lee ),김정구 ( Jeong Gu Kim ) 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 2012 한국미생물·생명공학회지 Vol.40 No.1
A new method for the high throughput screening of antagonistic substances against rice pathogens using rice leaf explants was developed. This method can be used to confirm the activities of any compound or mixture suppressing rice bacterial blight (BB) before field tests. Xanthomonas oryzae pv. oryzae (Xoo) culture medium was distributed in 96 well plates with equally sized explants and the active compounds were added to the wells. The strength suppressing BB was converted into an area percent of the lesion on the rice explants. The explants under BB suppressing activity remained uninfected maintaining their actual green color, while infected explants exhibited pale yellow-colored lesions. Based on the results, this method seems to be faster and easier, dose-dependent, and can be performed all-at-once with a small amount of unspecified compounds. This method also has the potential to be applied to inspection activities for the suppression of other waterborne crop diseases.
혈청 콜레스테롤과 식이 섭취와의 연관성에 대한 단면적 조사
박혜순,이규희,박샛별 한국지질학회 1997 韓國脂質學會誌 Vol.7 No.1
Background: Hyperlipidemia is a well recognized risk factor of coronary heart disease. In Korea, hypercholesterolemic patients are increasing now by westernized dietary pattern. Serum lipid level is known to be related to the dietary intake. The studies about the relationship of serum cholesterol and diet are not sufficient in Korea. We conducted this study to show the relationship of serum cholesterol and dietary intake among the Korean. Methods: Four hundred sixty nine patients who filled up a semi-quantitative food frequency questionnaire in the Lifelong Health Monitoring Program(LHMP) clinic were evaluated about serum cholesterol level. Dietary cholesterol amount, calories, carbohydrate, protein, fat content were calculated by the nutrition analysis program, and then Pearson correlation coefficients was used to evaluate the correlation between serum cholesterol and dietary contents. Results: Pearson correlation coefficients between serum cholesterol and dietary cholesterol, fat, calories are 0.03(P=0.6187), 0.05(P=0.3603), 0.08(P=0.1532) in men and -0.04(P=0.5873), -0.14(P=0.0611), -0.17(P=0.0215) in women. Conclusion: It seemed to be there is no correlation between serum cholesterol and dietary intake in both men and women. There is no need to restrict dietary cholesterol and fat in persons who have normal serum cholesterol level. We have to assess nutrient intake and manage by individualized approach for hypercholesterolemic patients.