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Lee, Yeon-Ju,Han, Saem,Lee, Hyi-Seung,Kang, Jong Soon,Yun, Jieun,Sim, Chung J.,Shin, Hee Jae,Lee, Jong Seok American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.9
<P>Seventeen bromotyrosine-derived metabolites, including eight new compounds, were isolated from a Micronesian sponge of the genus <I>Suberea</I>. Four of the new compounds were psammaplysin derivatives (<B>10</B>–<B>13</B>), and the other four were ceratinamine derivatives (<B>14</B>–<B>17</B>). Of the compounds obtained, the psammaplysins exhibited cytotoxicity against human cancer cell lines (GI<SUB>50</SUB> values down to 0.8 μM), while the ceratinamine and moloka’iamine analogues showed almost no activity. These results suggest that the spirooxepinisoxazoline ring system is a requirement for cytotoxicity and, therefore, may serve as an attractive molecular scaffold for the development of a potent anticancer agent.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-9/np400448y/production/images/medium/np-2013-00448y_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400448y'>ACS Electronic Supporting Info</A></P>
Lee, Han Saem,Kim, Ji Yun,Lee, Won Il,Kim, Sung Jin,Ko, Min Ji,Jeong, Sunjoo,Park, Keerang,Choe, Han,Lee, Heuiran Spandidos Publications] 2011 Oncology letters Vol.2 No.6
<P>Recombinant adeno-associated virus serotype 5 (rAAV5) is considered to be a promising gene transfer vehicle. However, preferential gene delivery to the tumor remains a requirement for cancer treatment. We generated rAAV5 mutants bearing tumor marker-binding peptides and analyzed their properties as viral vectors, as well as their transduction efficiencies and preferential antitumoral potencies. All of the mutants were successfully produced. Transduction analyses showed that rAAV5 mutants harboring tumor-homing peptides, including RGD and TnC, transduced human cancer cells expressing corresponding receptors on their surfaces. RGDS peptides and TnC antibodies significantly suppressed transduction by rAAV5-RGD and rAAV5-TnC. Cytotoxicity was evident upon transfer of HSV-TK to cells by re-targeted rAAV5. These results provide evidence that rAAV5 vectors, genetically armed with tumor-targeting ligands, preferentially infect human cancer cells harboring the corresponding receptors, thereby inducing antitumoral effects. Further optimization of rAAV5 mutant viruses should thus facilitate practical exploitation of these vectors for gene-based cancer treatment.</P>
( Han-saem Lee ),( Eun-ju Yang ),( Taeho Lee ),( Kyung-sik Song ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2
Enzyme fermentation is a type of food processing technique generally used to improve the biological activities of food and herbal medicines. In this study, a Syzygii Flos (clove) extract was fermented using laccase derived from Trametes versicolor (LTV). The fermented clove extract showed greater neuroprotective effects against glutamate toxicity on HT22 than the non-fermented extract did. HPLC analysis revealed that the eugenol (1) and dehydrodieugenol (2) contents had decreased and increased, respectively, after fermentation. The content of 2 peaked at 1 h after fermentation to 103.50 ± 8.20 mg/g<sub>ex</sub> (not detected at zero time), while that of 1 decreased to 79.54 ± 4.77 mg/g<sub>ex</sub> (185.41 ± 10.16 mg/g<sub>ex</sub> at zero time). Compound 2 demonstrated promising HT22 neuroprotective properties with inhibition of Ca<sup>2+</sup> influx, the overproduction of intracellular reactive oxygen species, and lipid peroxidation. In addition, LTV showed the best fermentation efficacy compared with laccases derived from Pleurotus ostreatus and Rhus vernicifera.
Han Saem Lee,Da So Mi Park,Yuhoon Hwang,Jong Gil Ha,Hyung Sang Shin 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.3
This work describes highly efficient recovery and selective leaching of Zn from electric arc furnace dust (EAFD) with different physicochemical properties, induced by acid leaching at ambient conditions. The chemical compositions, mineralogical phases, and particle sizes of the EAFDs were analyzed and compared. The effects of leaching time, liquid/solid ratio, acid type, and acid concentration on the selective leaching of Zn were also studied. The EAFD with high Fe/Zn ratio (> 1, EAFD₃) was richer in ZnFe₂O₄ and exhibited larger particle size than samples with low Fe/Zn ratio (< 1, EAFD1,2). ANOVA analysis revealed that the Fe/Zn ratios of the EAFDs also have a significant effect on Zn extraction (p < 0.005). Selective leaching of Zn with minimum Fe dissolution was obtained at pH > 4.5, regardless of other parameters or sample properties. The maximum Zn extraction rate obtained by the pH control was over 97% for EAFD₁ and EAFD₂, 76% for EAFD₃, and 80% for EAFD₄. The present results confirm that the Fe/Zn ratio can be used to identify EAFDs that permits facile and high-yield Zn recovery, and pH can be used as a process control factor for selective leaching of Zn regardless of any differences in the properties of the EAFD sample.
Determination of hesperidin in mixed tea by HPLC
Saem Han(한샘),So-Youn Mok(목소연),Hye-Min Kim(김혜민),Jeong-Min Lee(이정민),Dong Gu Lee(이동구),Sook-Young Lee(이숙영),Jongkee Kim(김종기),Sun-Ju Kim(김선주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2
The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 ㎚). The How rate was kept constant at 1.0 ㎖/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 ㎎/g, respectively).
[$Ca^{2+}-activated\;K^+$ Currents of Pancreatic Duct Cells in Guinea-pig
Lee, Han-Wook,Li, Jing Chao,Koo, Na-Youn,Piao, Zheng Gen,Hwang, Sung-Min,Han, Jae-Woong,Choi, Han-Saem,Lee, Jong-Heun,Kim, Joong-Soo,Park, Kyung-Pyo The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6
There are numerous studies on transepithelial transports in duct cells including $Cl^-$ and/or $HCO_3^-$. However, studies on transepithelial $K^+$ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of $K^+$ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both $Cl^-$ and $K^+$ conductance were found with KCI rich pipette solutions. When the bath solution was changed to low $Cl^-$, reversal potentials shifted to the negative side, $-75{\pm}4\;mV$, suggesting that this current is dominantly selective to $K^+$. We then characterized this outward rectifying $K^+$ current and examined its $Ca^{2+}$ dependency. The $K^+$ currents were activated by intracellular $Ca^{2+}$. 100 nM or 500 nM $Ca^{2+}$ in pipette significantly (P<0.05) increased outward currents (currents were normalized, $76.8{\pm}7.9\;pA$, n=4 or $107.9{\pm}35.5\;pA$, n=6) at +100 mV membrane potential, compared to those with 0 nM $Ca^{2+}$ in pipette $(27.8{\pm}3.7\;pA,\;n=6)$. We next examined whether this $K^+$ current, recorded with 100 nM $Ca^{2+}$ in pipette, was inhibited by various inhibitors, including $Ba^{2+}$, TEA and iberiotoxin. The currents were inhibited by $40.4{\pm}%$ (n=3), $87.0{\pm}%$ (n=5) and $82.5{\pm}%$ (n=9) by 1 mM $Ba^{2+}$, 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular $Ca^{2+}$. The $K^+$ current may play a role in secretory process, slnce recycling of $K^+$ is critical for the initiation and sustaining of $CI^-$ or $HCO_3^-$ secretion in these cells.
P230 : Treatment of actinic keratoses with 5% topical imiquimod
( Han Saem Kim ),( Ju Yeon Choi ),( Jung In Kim ),( Jung Min ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Heun Joo Lee ),( Yoon Hwan Kim ),( Jae Hui Nam ),( Ho Joo Jung ),( Ji Hye Park ),( Ga Young Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: An actinic keratosis (AK) is a pre-malignant cutaneous lesion that frequently manifests in sun-exposed areas. Various treatments may also be used including cryosurgery, photodynamic therapy, topical imiquimoid and 5-FU, and surgical excision. Objectives: This study was performed to evaluate the efficacy and safety of 5% topical imiquimod for AKs. Methods: We performed a prospective study for 52 AK lesions from 22 patients who were treated by 5% topical imiquimod. Imiquimod was applied every other day for 16 weeks. At 4-week visit, assessment of AK lesions was performed, and lesions showing noncomplete response were retreated for an additional cycle for 4 weeks. Clinical response rate and adverse events were assessed at each study visit. Results: The clinical complete response rate of AK lesions was 8.3% at 4-week, 17.0% at 8-week, 31.7% at 12-week and 41.5% at 16-week. Local skin reactions were reported by 45.5% (10 of 22) of patients. Erythema was experienced by 22.7% (5 of 22) of patients and burning sensation (18.2%), oozing (9.1%) and ulceration (4.5%) were investigated. However, only one participant discontinued from the treatment period because of local skin reactions. Conclusion: 5% topical imiquimod is an effective and well-tolerated treatment for AK. Further investigation with a large group of patients is essential to evaluate the efficacy and safety of 5% topical imiquimod in AK of Korean population.