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Lee, Han Saem,Kim, Ji Yun,Lee, Won Il,Kim, Sung Jin,Ko, Min Ji,Jeong, Sunjoo,Park, Keerang,Choe, Han,Lee, Heuiran Spandidos Publications] 2011 Oncology letters Vol.2 No.6
<P>Recombinant adeno-associated virus serotype 5 (rAAV5) is considered to be a promising gene transfer vehicle. However, preferential gene delivery to the tumor remains a requirement for cancer treatment. We generated rAAV5 mutants bearing tumor marker-binding peptides and analyzed their properties as viral vectors, as well as their transduction efficiencies and preferential antitumoral potencies. All of the mutants were successfully produced. Transduction analyses showed that rAAV5 mutants harboring tumor-homing peptides, including RGD and TnC, transduced human cancer cells expressing corresponding receptors on their surfaces. RGDS peptides and TnC antibodies significantly suppressed transduction by rAAV5-RGD and rAAV5-TnC. Cytotoxicity was evident upon transfer of HSV-TK to cells by re-targeted rAAV5. These results provide evidence that rAAV5 vectors, genetically armed with tumor-targeting ligands, preferentially infect human cancer cells harboring the corresponding receptors, thereby inducing antitumoral effects. Further optimization of rAAV5 mutant viruses should thus facilitate practical exploitation of these vectors for gene-based cancer treatment.</P>
( Han-saem Lee ),( So-hyun Chon ),( Min-a Kim ),( Jeong-eun Park ),( Yu-mi Lim ),( Eun-jeong Kim ),( Eun-kyung Son ),( Sang-jun Kim ),( Jai-hyun So ) 한국응용생명화학회(구 한국농화학회) 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.1
The ethyl acetate fraction of Bat Faeces (Ye Ming Sha: natural products used in Chinese Medicine) after fermentation (EFBF-AF) showed enhanced anti-oxidative effects in 2,2-diphenyl- 1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) diammonium salt assays. Fermentation of the Bat Faeces by using the crude enzyme extract from Aspergillus kawachii, significantly increased the anti-inflammatory effects. Fermented Bat Faeces markedly inhibited nitric oxide production, inducible nitric oxide synthase, and cyclooxygenase-2 expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The EFBF-AF reduced the nuclear translocation of nuclear factor kappa B (NF-κB) via IKKα and IκBα phosphorylation, and decreased the phosphorylated the extracellular signal-regulated kinases (ERK) and p38 expression in LPS-treated RAW 264.7 macrophages. In addition, the EFBF-AF suppressed the expression of pro-inflammatory genes, such as interleukin-1β, interleukin-6, and tumor necrosis factor-α. These results suggest that fermented Bat Faeces may suppress pro-inflammatory responses in LPSstimulated RAW 264.7 macrophages cells via ERK, p38 mitogenactivated protein kinase and NF-κB signaling pathways.
Lee, Yeon-Ju,Han, Saem,Lee, Hyi-Seung,Kang, Jong Soon,Yun, Jieun,Sim, Chung J.,Shin, Hee Jae,Lee, Jong Seok American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.9
<P>Seventeen bromotyrosine-derived metabolites, including eight new compounds, were isolated from a Micronesian sponge of the genus <I>Suberea</I>. Four of the new compounds were psammaplysin derivatives (<B>10</B>–<B>13</B>), and the other four were ceratinamine derivatives (<B>14</B>–<B>17</B>). Of the compounds obtained, the psammaplysins exhibited cytotoxicity against human cancer cell lines (GI<SUB>50</SUB> values down to 0.8 μM), while the ceratinamine and moloka’iamine analogues showed almost no activity. These results suggest that the spirooxepinisoxazoline ring system is a requirement for cytotoxicity and, therefore, may serve as an attractive molecular scaffold for the development of a potent anticancer agent.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-9/np400448y/production/images/medium/np-2013-00448y_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400448y'>ACS Electronic Supporting Info</A></P>
Isolation and Identification of Phytochemical Constituents from Taraxacum coreanum
Lee, Sul-Lim,Han, Saem,Kim, Hye-Min,Lee, Jeong-Min,Mok, So-Youn,Lee, Sang-Hyun The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
Phytochemical constituents were isolated from the aerial parts of Taraxacum coreanum (Asteraceae) by repeated column chromatography and prep-HPLC. Their structures were identified as ${\beta}$-sitosterol (1), daucosterol (2), taraxasteryl acetate (3), chrysoeriol (4), diosmetin (5), luteolin (6), luteolin-7-O-glucoside (7), esculetin (8), and 5-hydroxypyrrolidin-2-one (9) by the interpretation of spectroscopic analyses including MS, $^1H$-, and $^{13}C$-NMR. This is the first report on the isolation of compounds 1-9 from T. coreanum. Among the compounds, 5 and 9 were isolated for the first time from Taraxacum.
[$Ca^{2+}-activated\;K^+$ Currents of Pancreatic Duct Cells in Guinea-pig
Lee, Han-Wook,Li, Jing Chao,Koo, Na-Youn,Piao, Zheng Gen,Hwang, Sung-Min,Han, Jae-Woong,Choi, Han-Saem,Lee, Jong-Heun,Kim, Joong-Soo,Park, Kyung-Pyo The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6
There are numerous studies on transepithelial transports in duct cells including $Cl^-$ and/or $HCO_3^-$. However, studies on transepithelial $K^+$ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of $K^+$ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both $Cl^-$ and $K^+$ conductance were found with KCI rich pipette solutions. When the bath solution was changed to low $Cl^-$, reversal potentials shifted to the negative side, $-75{\pm}4\;mV$, suggesting that this current is dominantly selective to $K^+$. We then characterized this outward rectifying $K^+$ current and examined its $Ca^{2+}$ dependency. The $K^+$ currents were activated by intracellular $Ca^{2+}$. 100 nM or 500 nM $Ca^{2+}$ in pipette significantly (P<0.05) increased outward currents (currents were normalized, $76.8{\pm}7.9\;pA$, n=4 or $107.9{\pm}35.5\;pA$, n=6) at +100 mV membrane potential, compared to those with 0 nM $Ca^{2+}$ in pipette $(27.8{\pm}3.7\;pA,\;n=6)$. We next examined whether this $K^+$ current, recorded with 100 nM $Ca^{2+}$ in pipette, was inhibited by various inhibitors, including $Ba^{2+}$, TEA and iberiotoxin. The currents were inhibited by $40.4{\pm}%$ (n=3), $87.0{\pm}%$ (n=5) and $82.5{\pm}%$ (n=9) by 1 mM $Ba^{2+}$, 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular $Ca^{2+}$. The $K^+$ current may play a role in secretory process, slnce recycling of $K^+$ is critical for the initiation and sustaining of $CI^-$ or $HCO_3^-$ secretion in these cells.
[P417] A case of rheumatoid neutrophilic dermatosis mimicking herpes simplex infection
( Han-saem Kim ),( Junghwa Yang ),( Yun Ho Lee ),( Jung Yup Kim ),( Sunmin Yim ),( Jae Yun Lim ),( Ju-yeon Choi ),( Joon Hong Min ),( Young Jun Choi ),( Jae-hui Nam ),( Ga-young Lee ),( Won-serk Kim ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1
Rheumatoid arthritis (RA) is an autoimmune inflammatory disorder. Various cutaneous manifestations are associated with RA, including rheumatoid nodule, vasculitis, palisading granulomatous dermatitis, and neutrophilic disorders. Rheumatoid neutrophilic dermatitis (RND) occurs in patients with active and severe RA, and vesicular-type RND is rarely reported, which predominantly involves the lower extremities. A 66-year-old female presented a 6-year history of RA. She had successfully treated with the combination of systemic prednisolone and methotrexate. At the beginning of 2015, erythematous vesicles with crust and erosion suddenly began to spread symmetrically on the both thighs. The lesion was accompanied with intermittent pain. The patient was initially treated with oral acyclovir and a mid-potent topical steroid, based on the diagnosis of herpes simplex. The lesions, however, were recurrent every month despite taking medications. A year later, a skin biopsy was performed. The histological examination showed intracorneal pustules and dense dermal inflammatory infiltrates, consisting mainly of adundant neutrophils. There were endothelial swelling and luekocytoclasis, but no vasculitis. The patient was then started on leflunomide for the control of RA with satisfactory preliminary skin response. The based on the clinical and histological features, RND was diagnosed.