Immunostimulatory Effects of β-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes

Ji-Mi Kim,주홍구 대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.4

We investigated the effects of β-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that β-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. β-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that β-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. W e also investigated the IL-2 dependency of β-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, β-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and β-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that β-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of β-glucan in research fields.

Immunostimulatory Effects of ${\beta}$-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes

Kim, Ji-Mi,Joo, Hong-Gu The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.4

We investigated the effects of ${\beta}$-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that ${\beta}$-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. ${\beta}$-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that ${\beta}$-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of ${\beta}$-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, ${\beta}$-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and ${\beta}$-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that ${\beta}$-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of ${\beta}$-glucan in research fields.

Immunostimulatory Effects of b-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes

Ji-Mi Kim,Hong-Gu Joo 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.4

We investigated the effects of Ղ-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that Ղ-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. Ղ-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that Ղ-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of Ղ-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, Ղ-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and Ղ-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that Ղ-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of Ղ-glucan in research fields.

Effects of MOK, a pharmacopuncture medicine, on the TH1/TH2 immune response and antioxidation in Con A-stimulated primary mouse splenocytes

황지혜 대한침구의학회 2017 대한침구의학회지 Vol.26 No.3

Objectives : In this study, we investigated the immunomodulatory and antioxidant effect of MOK, a pharmacopuncture medicine, in concanavalin A (Con A)-stimulated mouse splenocytes. Methods : Primary splenocytes were isolated from ICR mice. The splenocytes were treated with MOK extract (1.25, 2.5, 5, 10, and 20 mg/mL) for 30 min and then stimulated with Con A (200ng/mL) for the indicated times. Cell viability of the splenocytes was measured using an MTT assay. The mRNA expression of Th1/Th2 cytokines (IFN-γ, IL-4, IL-10, and Foxp3) and antioxidant enzymes (HO-1 and MnSOD) was measured by RT-PCR. Results : Addition of MOK extract at 2.5, 5, and 10 mg/mL in Con A-stimulated splenocytes significantly decreased the production of IFN-γ and significantly increased the expression of IL-4, IL-10, and Foxp3 mRNA. MOK extract also increased the mRNA expression of HO-1 and MnSOD in splenocytes. Conclusion : MOK extract modulated the Th1/Th2 immune response via the regulation of cytokine levels in splenocytes and exerted an antioxidant effect via the upregulation of antioxidant proteins.

Effects of MOK, a pharmacopuncture medicine, on the TH1/TH2 immune response and antioxidation in Con A-stimulated primary mouse splenocytes

Hwang, Ji Hye Korean AcupunctureMoxibustion Medicine Society 2017 대한침구의학회지 Vol.26 No.3

Objectives : In this study, we investigated the immunomodulatory and antioxidant effect of MOK, a pharmacopuncture medicine, in concanavalin A (Con A)-stimulated mouse splenocytes. Methods : Primary splenocytes were isolated from ICR mice. The splenocytes were treated with MOK extract (1.25, 2.5, 5, 10, and 20 mg/mL) for 30 min and then stimulated with Con A (200 ng/mL) for the indicated times. Cell viability of the splenocytes was measured using an MTT assay. The mRNA expression of Th1/Th2 cytokines ($IFN-{\gamma}$, IL-4, IL-10, and Foxp3) and antioxidant enzymes (HO-1 and MnSOD) was measured by RT-PCR. Results : Addition of MOK extract at 2.5, 5, and 10 mg/mL in Con A-stimulated splenocytes significantly decreased the production of $IFN-{\gamma}$ and significantly increased the expression of IL-4, IL-10, and Foxp3 mRNA. MOK extract also increased the mRNA expression of HO-1 and MnSOD in splenocytes. Conclusion : MOK extract modulated the Th1/Th2 immune response via the regulation of cytokine levels in splenocytes and exerted an antioxidant effect via the upregulation of antioxidant proteins.

Radioprotective Effect of Hesperetin against γ-Irradiationinduced DNA Damage and Immune Dysfunction in Murine Splenocytes

강정애,윤선혜,Jong Kook Rho,장범수,Dae Seong Choi,Dong-Eun Lee,변의백,Jongho Jeon,박상현 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.suppl1

This study was conducted to evaluate the preventive effect of hesperetin against radiationinduced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 μM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to γ-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.

調經種玉湯이 Immobilization Stress를 負荷한 C57BL/6J 생쥐의 免疫系에 미치는 影響

민경헌,김주연,김송백,조한백 대한한방부인과학회 2004 大韓韓方婦人科學會誌 Vol.17 No.4

The purpose of this research was to investigate the effects of Chokyungjongoktang water extract(CJT) on immune system by immobilization stress in C57BL/6J mice. CJT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, CJT did not affect the viability of thymocytes and enhanced the viability of splenocytes decreased by immobilization. Also, CJT increased the viability of thymocytes and decreased the viability of splenocytes in vitro. CJT did not affect DNA fragmentation of thymocytes and splenocytes increased by immobilization stress. CJT increased the population of thymic CD4+ cells increased by immobolization stress, and decreased the population of Thy1+ cells increased by immobilization stress and increased the population of splenic CD8+ cells increased by immobilization stress. CJT enhenced the production of -interferon and IL-2 decreased by immobilization stress. Also, CJT decreased the phagocytic activity decreased by immobilization stress and decreased the level of nitric oxide decreased by immobilization stress. These results indicate that CJT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level, and enhancement of viability of splenocytes and cytokines production from Th1 cells in splenocytes.

Immunoregulation Effect of KamiBohuh-tang

Park, Kyung Mi,Jung, Jin Hong,Yoo, Dong Youl 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.1

The purpose of this study is to prove the efficacy of KamiBohuh-tang(KBT) on immunoregulation and the possibility of KBT as an immunoadjuvant. KBT with solid feed was administered orally once a day for 7 days to an experimental group, a solution of salt and solid feed without KBT to a control group. After a week T cell, B cell, cytokines, nitric oxide and phagocytic activity are measured. KBT enhanced the proliferation of splenocytes and the subpopulation of Th cells in splenic T-lymphocytes, but did not affect the proliferation of thymocytes. KBT decreased the subpopulation of T-lymphocytes in splenocytes. KBT enhanced the production of interferon-γ, interleukin-2, interleukin-4 in mice serum and the phagocytic activity in peritoneal macrophages but it suppressed the production of nitric oxide. These results suggest that KBT is a potent prescription on immune response via the increase of the proliferation of splenocytes, the production of cytokines from splenic Th cells and the phagocytic activity in vivo.

Stimulating effects on mouse splenocytes of glycoproteins from the herbal medicine <i>Atractylodes macrocephala</i> Koidz.

Lee, J.-C.,Lee, K.-Y.,Son, Y.-O.,Choi, K.-C.,Kim, J.,Kim, S.-H.,Chung, G.-H.,Jang, Y.-S. Elsevier 2007 Phytomedicine Vol.14 No.6

<P><B>Abstract</B></P><P>A traditional herbal medicine, <I>Atractylodes macrocephala</I> Koidz. (AMK), has long been used as a digestive and tonic. Recent investigations have suggested its potential ability in stimulating immune responses, although a scientific basis for this activity has not yet been elucidated. Based on previous results showing that the activity might be due to proteins, we purified protein samples from an original sample preparation of AMK and examined the stimulating ability of the protein samples on mouse splenocytes. The sample treatment markedly stimulated lymphocyte proliferation, antibody production, and cytokine secretion in mouse splenocytes. In particular, the samples showed the ability to induce the preferential stimulation of Th1 type, rather than Th2 type T lymphocytes. Stimulating activity of the samples was associated closely with glycoprotein(s) with molecular weights of around 30kDa, especially with carbohydrate moiety rather than with protein residues of the glycoprotein(s). Our findings suggest that the glycoprotein(s) might play critical roles in modulating immune-response induction, and could potentially be used as medicinal and pharmacological agents.</P>

Rumex crispus and Cordyceps militaris Mixture Ameliorates Production of Pro-Inflammatory Cytokines Induced by Lipopolysaccharide in C57BL/6 Mice Splenocytes

Eui-Seong Park,Gyl-Hoon Song,Seung-Hee Kim,Seung-Min Lee,Yong-Gyu Kim,Yaung-lee Lim,Soon Ah Kang,Kun-Young Park 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.4

Rumex crispus (Rc) and Cordyceps militaris (Cm) mixture (Rc-Cm; AST2017-01) ameliorated production of proinflammatory cytokines, inflammation-related genes, and nitric oxide (NO) induced by lipopolysaccharide (LPS) in mouse splenocytes. Rc-Cm (6:4) and Taemyeongcheong (commercial healthy drink containing Rc-Cm) were co-administered along with LPS. Rc-Cm inhibited production of tumor necrosis factor α, interferon γ, interleukin (IL)-1β, and IL-6 in LPSinduced splenocytes. However, levels of inflammatory cytokines were elevated in the absence of LPS treatment. Rc-Cm significantly suppressed mRNA expression of IL-1β, IL-6, and the inflammation-related genes inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2), as well as NO production upon LPS co-treatment. Whereas Rc-Cm increased mRNA expression of IL-1β, and IL-6, but did not up-regulate expression of iNOS and COX-2, or increase NO production without LPS co-treatment. Therefore, treatment of Rc-Cm to LPS-induced splenocytes ameliorated induction of pro-inflammatory cytokines, inflammation-related genes, and NO production. In the absence of LPS, Rc-Cm treatment up-regulated pro-inflammatory cytokines but did not alter expression of the inflammation-related genes iNOS and COX-2 or NO production. These results indicate that the natural phytochemicals chrysophanol and cordycepin in Rc-Cm promote antiinflammatory activities and immune cell responses.