Radioprotection Effects to the Radiation Side Effect in EEM (Extracts of Edible Mushrooms)

Gu, Yeun-Hwa,Masaki, Matsumori,Suzuki, Ikukatu,Takeo, Hasegawa,Yuuichi, Ukawa,Hitoshi, Tawaraya,Ikuo, Hayashi,Ahn, Kyoo-Seok INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 2003 JOURNAL OF ORIENTAL MEDICINE Vol.6 No.1

Presently, raditherapy is often used alone or in combination with anti-cancer medicine as a treatment for cancer. Chemosynthesis has been used as a radiation protection medicine. But, its use has been restricted due to serious side effects. ICR mice have often been used for malformation experiments as they were used in this research. Here, EEM (extracts of edible mushrooms (and extraction from Flammulina velutires and lyuophyllum ulmarium)), a natural product, was used as a radioprotective agent and its effects were examined. A decline in the fetus malformation rate was recognized when giveing EEM before the radiation irradiation. Moreover, a repression in the decrease of fetus weight was recognized, and the effect on radiation protection of EEM was explained at the individual level. And, I had the effect on protection that faced a corpuscle cell by the radiation by giving EEM was explained in the same way too. As for the cell level as well, it was proved that there was an effect on protection against radiation irradiation in the same way as the individual level. Furthermore, an examination was done about the effects on the external malformation to the radiation, the embryonic death rate and the sensitivity of the lymphocyte at the organogenesis stage. Furtheromore, the study was done about the effects on the malformation to the radiation, the embryonic death rate and the sensitivity of the lymphocyte at the fetus of the organogenesis stage.

고려인삼의 방사선 방어효과에 대한 연구현황과 전망

남기열,박종대,최재을 韓國藥用作物學會 2011 한국약용작물학회지 Vol.19 No.4

Pharmacological effects of Panax ginseng have been demonstrated in cardiovascular system, endocrine secretion and immune system, together with antitumor, anti-stress and anti-oxidant activities. Modern scientific data show protective effect of ginseng against bone marrow cell death, increased survival rate of experimental animals, recovery of hematopoietic injury, immunopotentiation, reduction of damaged intestinal epithelial cells, inhibition of mutagenesis and effective protection against testicular damages, caused by radiation exposure. And also, ginseng acts in indirect fashion to protect radical processes by inhibition of initiation of free radical processes and thus reduces the radiation damages. The research has made much progress, but still insufficient to fully uncover the action mechanism of ginseng components on the molecule level. This review provides the usefulness of natural product, showing no toxic effects, as an radioprotective agent. Furthermore, the further clinical trials on radioprotection of ginseng need to be highly done to clarify its scientific application. The effective components of ginseng has been known as ginsenosides. Considering that each of these ginsenosides has pharmacological effect, it seems likely that non-saponin components might have radioprotective effects superior to those of ginsenosides, suggesting its active ingredients to be non-saponin series. These results also show that the combined effects of saponin and non-saponin components play an important role in the radioprotective effects of ginseng.

A study of the radioprotection effect of guarana (Paullinia cupana) on the fetuses of ICR mice THE RADIATION PROTECTION EFFECTS OF GUARANA

Gu, Yeun-Hwa,Hasegawa, Takeo,Suzuki, Ikukatsu,Yamamoto, Youichi,Yoon, Yeog-Byung,Rhee, Soo-Yong Korean Association for Radiation Protection 2001 방사선방어학회지 Vol.26 No.4

Guarana, a tropical plant is found in powdered for in health food and is very popular soft drink in Brazil as an energy feaster with its high caffeine contents. We examined its radioprotection effects during organogenesis stages of ICR mice by malformations rate and cellular lead 8 the embryo by radiation and analyzed the mechanism of the radioprotection effects in the fetal of ICR mice. The results of this study showed that Guarana reduced clearly the embryonic death rate and teratogenesis rate by radiation. Its radioprotection effect inject be related with its radioprotection effect might be related with its antioxidant effect or free radical scavenger. We need to exposure the Guarana as a potential radioprotection agent. Therefore, we investigated about radiation effects by Guarana using to mice experiments in this paper.

Radioprotection Effects and the Anti-Cancer Effects of the Species (Miroku-Tea)

Gu, Yeun-Hwa,Suzuki, Ikukatu,Hasegawa, Takeo,Hayashi, Ikuo,Hitoshi, Tawaraya,Ahn, Kyoo-Seok INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 2001 JOURNAL OF ORIENTAL MEDICINE Vol.6 No.1

In recent years the effectiveness of species (Miroku-Tea) as an anti-cancer, anti0biotic effect and anti-virus effect has become more widely known. Furthermore the beneficial effects of species (Miroku-Tea) leaves have also been studied. In this study, we investigated measurement of blood cells, the NK cell activity, the radio-protective and anti-cancer effects of species (Miroku-Tea) extract. The leucocytes and the lymphocyte were transietnly increased after injection of species (Miroku-Tea). Anti-tumor effect was occurred after the injuection of species (Miroku-Tea). NK cells activity increased 2 times after the injection of species (Miroku-Tea) compared with the control group. Radiation protection occurred from the inhibition of body weight loss and also a prolonged the surviving fraction after the injection of species (Miroku-Tea).

생맥산(生脈散)의 방사선 보호효과 : 생쥐 소낭세포 재생과 Apoptosis에 미치는 영향

김현경,윤상협,류봉하,김진성,Kim, Hyun-Kyung,Yoon, Sang-Hyub,Ryu, Bong-Ha,Kim, Jin-Sung 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.2

Backgrounds & Objects: The aim of this study was to investigate the radioprotective effect of Shengmai-san(SMS), a herbal medicine, on mice jejunal crypt cell survival and Apoptosis. Methods: Mice were devided into 4 groups according to radiation dose and SMS treatment: Normal was the group without irradiation. Control was the group treated with D.W before 10 Gy irradiation. SMS 2.9 was sample group treated with 2.9 mg/10 g of SMS extract before 10 Gy irradiation and SMS 29 was sample group treated with 29 mg/10 g of SMS extract before 10 Gy irradiation. And Each group were sacrificedat 24 hours and 72 hours after irradiation. To analyze the crypt survival, hematoxylin-eosin staining was used and to analyze the apoptosis, the TUNEL assay was done. Results: 1. From the microcolony survival assay, the SMS 2.9 and SMS 29 showed the radioprotective effect with a statistical significance compared to the control group at 24 hr (P < 0.01) and 72 hr (p < 0.001) after 10 Gy irradiatien. And the differences of radioprotective effect between SMS 2.9 and SMS 29 were net significant. 2. The results of the TUNEL assay showed that the apoptotic index in SMS 2.9 and SMS 29 was significantly decreased, as compared to the control group at both 24 hr ( p < 0.01) and 72 hr (SMS 2.9 : p < 0.001. SMS 29 : P < 0.01) after 10Gy irradiation And the differences of between SMS 2.9 and SMS 29 were not significant. Conclusions: It could be suggested that the Shengmai-san has a prominent Protective effect in mice intestines against the radiation damage. And the radieprotective effect seems to be related to inhibition of the apoptosis.

A study of the radioprotection effect of guarana (Paullinia cupana) on the fetuses of ICR mice : THE RADIATION PROTECTION EFFECTS OF GUARANA

Gu, Yeunhwa,Takeo Hasegawa,Ikukatsu Suzuki,Youichi Yamamoto,Yoon, Yeogbyung,Rhee, Soo-Yong 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.4

Guarana, tropical plant is found in powdered for in health food and is very popular soft drink in Brazil as an energy feaster with its high caffeine contents. We examined its radioprotection effects during organogenesis stages of ICR mice by malformations rate and cellular lead 8 the embryo by radiation and analyzed the mechanism of the radioprotection effects in the fetal of ICR mice. The results of this study showed that Guarana reduced clearly the embryonic death rate and teratogenesis rate by radiation. Its radioprotection effect inject be related with its radioprotection effect might be related with its antioxidant effect of free radical scavenger. We need to exposure the Guarana as a potential radioprotection agent. Therefore, we investigated about radiation effects by Guarana using to mice experiments in this paper.

A Study of the radioprotection effect of macro-glucan (β-1-3glucan) on thefetuses of ICR mice

Gu, Yeun-hwa,Hasegawa, Takeo,Suzuki, Ikukatsu,Hayashi, Ikuo,Ahn, Kyoo-Seok,Tawaraya, Hitoshi INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 2000 JOURNAL OF ORIENTAL MEDICINE Vol.5 No.1

In this study abundant malformations of ICR mice in the organogenesis stages were observed and the radioprotection effect of Macro-Glucan(β-1-3glucan) was shown. This research examined the radioprotection effect of Macro-Glucan(β-1-3glucan) and its teratogenic properties. The reason it has a preventive effect on a tall fetus was examined in the research. We examined the determination stage (organogenesis), and pointed to malformation toward the radiation in the enviornmental agent to the malformaiton. Moreover, we examined the cellularis level of the embryos toward the radiation and analyzed the mechanism of the protection effect of the Macro-Glucan(-1-3glucan) from malformation induction by the radiation. Therefore, the purpose of this research is to get a foundation of data of the radioprotection effect of Macro-Glucan(-1-3glucan). This study produced the following results : It was made clear that Macro-Glucan(-1-3glucan) had a radioprotection effect against embryonic death. Radioprotection effect was recognized as the teratogenesis rate by Macro-Glucan(-1-3glucan)'s administering it before the exposure. Therefore, the subject faced radiation exposure by the Macro-Glucan(-1-3glucan) administration, and radiological protecting effect was made clear.

The Radioprotective Effect of Amphojel, Tathion, Elental and A.D.N. on the Small Intestine of Rats

Kim, Jong Woo,Lee, Han Jin CATHOLIC MEDICAL CENTER 1988 Bulletin of the Clinical Research Institute Vol.16/17 No.1

One of the major obstacles to adequate radiotherapy of abdomino-pelvic tumors is the high incidence of morbidity and mortality secondary to the effects of ionizing radiations on the gastrointestinal tract. More than 2000 drugs and major modifications of irradiation techniques have been employed clinically and experimentally to minimize these effects on normal tissue. The Amphojel^� (aluminum hydroxide) is a well known antacid and the Elental^� (Elemental Diet) is reported to have an less stimulating effect to the bile and pancreatic juice secretion. The Tathion^� (glutathione) and A. D. N.^� (desoxyribonucleic acid) have been reported to have radioprotective effect in the literatures. For the purpose of investigating the effect of radiation to the small intestine and providing information regarding radioprotective effects of Amphojel, Tathion , Elental and A. D. N. on the jejunum of rats, we measured the crypt counts per circumference and scored histological grading from the jejunal tissue samples. The results suggested that Amphojel^� and Elental^� used in this experiment appear to have radioprotective effects on the jejunum of the rats, as well as Tahion^� and A. D. N.^� that have been reported as radioprotective drugs, and to be more effective when they are administered at late period of radiotherapy, in which total radiation dose approaches to the tolerable dose.

Correlation of Increased Mortality with the Suppression of Radiation-inducible Microsomal Epoxide Hyderolase and Glutathione S-Transferase Gene Expression by Dexamethasone : Effects on Vitalmin C and E-Induced Radioprotection

Nam, Seon Young,Cho, Chul Koo,Kim, Sang Geon 德成女子大學校 藥學硏究所 1998 藥學論文誌 Vol.9 No.1

Previous studies in this laboratory have shown that γ-ray ionizing radiation in combination with oltipraz, a radioprotective agent, enhances hepatic microsomal epoxide hydrolase (mEH) and glutathione S-transferase (GST) expression. The present study was designed to investigate the effects of dexamethasone on the radiation inducible expression of mEH and rGST genes and on the vitamin C and E-induced radioprotective effects in association with the expression of the genes. Treatment of rats with a single dose of dexamethasone(0.01-1 ㎎/㎏, p.o.) caused a dose dependent decrease in the constitutive mEH gene expression at 24 hr. The radiation-inducible mEH mRNA level (threefold increase after 3 Gy γ irradiation) was decreased by 21% and 88% by dexamethasone at the doses of 0.1 and 1 ㎎/㎏, respectively. Although dexamethasone alone caused 2 to 5 fold increases in the hepatic rGSTA2 mRNA level, rats treated with dexamethasone prior to 3 Gy irradiation exhibited 80%-93% suppression in the radiation inducible increases in the rGSTA2 mRNA level. The inducible rGSTA3 and rGSTA5 mRNA levels were also significantly decreased by dexamethasone, whereas the rGSTM1 mRNA level was reduced to a lesser extent. Vitamin C and/or E, however, failed to enhance the radiation-inducible increases in hepatic mEH and rGST mRNA levels. Whereas rats exposed to 3 Gy irradiation with or without vitamin C treatment (30 or 200 ㎎/㎏/day, p.o., 2 days) exhibited∼threefold increases in the mEH and rGSTA2/3/5 mRNA levels relative to untreated animals, dexamethasone treatment (1 ㎎/㎏, p.o.) resulted in 64%-96% decreases in the mRNA levels at 24 hr. The inducible rGSTM1/2 mRNA levels in the vitamin C/E treated rats were ∼50% suppressed by dexamethasone. Although vitamin C and/or E treatment (200 ㎎/㎏/day, p.o., 2 days) improved the 30-day survival rates of the 8Gy γ-irradiated mice from 39% up to 74%, the improved survival rate of γ-irradiated animals was reduced to 30% by dexamethasone pretreatment (1 ㎎/㎏/day, 2 days). The mean survival time of dexamethasone-treated animals was reduced to ∼2days from 14days in the animals with total body irradiation alone. No significant hematologic changes were observed in mice at 10 days after dexamethasone plus γ-irradiation, as compared with irradiation alone. These results demonstrate that: dexamethasone substantially suppresses radiation-inducible mEH, rGSTA and rGSTM expression in the liver, vitamins C/E exhibit radioprotective effects without enhancing radiation-inducible mEH and GST gene expression; and inhibition of radiation inducible mEH and rGST gene expression in the vitamin C and E treated animals by dexamethasone was highly correlated with reduction in the survival rate and the mean survival time of γ-irradiated animals.

Radioprotective Effect of Lifukang, a Chinese Medicinal Plants Prescription

Jeong Hee Kim,Sung Ho Kim,Eun Ju Lee,Jiang Shen Gao,Zhen Yu Wu,Woong Chon Mar,Il Moo Chang 한국생약학회 1998 Natural Product Sciences Vol.4 No.1

We have examined in vitro and in vivo radioprotective effect of a Chinese medicinal plants prescription, Lifukang. Micronucleus assay was employed to evaluate in vitro radioprotective effect of Lifukang. In the presence of Lifukang, the frequencies of miconuclei were greatly reduced from 7.2 to 2.9, 1.6 and 1.6% at the concentrations of Lifukang from 0 to 2, 10 and 50㎍/ml, respectively. For in vivo assay, we monitered the incidences of apoptotic cells in mouse small intestine crypts and endogeneous spleen colonies. When Lifukang was administered to mice P.O. or I.P. at doses of 1㎎/ml in drinking water for 7 days or 0.3㎎/mouse 24 hrs prior to irradiations, respectively, the average numbers of apoptotic cells were reduced to 3.1 or 2.3, respectively, as compared to 4.4 acquired from untreated control experiments. In addition, in spleen colony assay, Lifukang increased the number of hematopoietic spleen colonies. When samples were administered after irradiation, better results were obtained. The numbers of spleen colonies were increased from 14 colonies to 18.3 or 19.6 colonies when Lifukang was given through P.O. (1㎎/ml in drinking water for 11 days) or I.P. (0.3㎎/mouse) after irradiation, respectively.