국내 유통 콩 및 녹두가루 제품의 품질 특성

우관식 ( Koan Sik Woo ),김미정 ( Mi-jung Kim ),심은영 ( Eun-yeong Sim ),김현주 ( Hyun-joo Kim ),이춘기 ( Choon Ki Lee ),전용희 ( Yong Hee Jeon ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.5

용도별 두류가루 제품의 품질기준 설정을 위해 시중에 유통되고 있는 두류가루 제품을 수집하여 품질 특성을 분석한 결과, 주원료의 산지는 국산은 23제품, 수입산은 19제품이었고, 백태가 주원료로 조사되었다. 제품의 가격은 국산이 수입산에 비해 생콩가루의 경우, 3.48배, 볶음콩가루의 경우는 3.34배, 녹두가루는 3.47배로 비쌌다. 국산 제품의 경우, 명도는 생콩가루가 볶음 콩가루에 비해 높았으며, 적색도와 황색도는 볶음 콩가루가 생콩가루에 비해 높았다. 수분함량은 생콩가루가 볶음 콩가루에 비해 높았으며, 조지방, 조단백질, 탄수화물 함량은 수입산 볶음 콩가루에서 높았다. 녹두의 회분과 조단백질 함량은 국산이 수입산에 비해 약간 높았다. 국산 콩가루 제품의 수분결합력은 볶음 콩가루가 생콩가루보다 높았으며, 용해도와 팽윤력은 유의적인 차이가 없었다. 시중유통 두류가루 제품의 품질 특성 간의 상관관계는 용해도를 제외하고 측정한 항목간에 높은 유의성을 보이는 것으로 조사되었다. Quality characteristics of commercially available soybean and mung-bean flours in Korea were evaluated relative to product information such as bean content, price, chromaticity, proximate composition, water binding capacity, swelling power and solubility. Origin of raw materials was 23 in domestic products and 19 in imported products. The price of the product was 3.48 times more for raw soybean flour, 3.34 times more for fried soybean flour, and 3.47 times more for mung bean flour compared to imported soybean. In the domestic products, the lightness of raw soybean flour was higher than roasted soybean flour. The redness and yellowness of roasted soybean flour were higher than raw soybean flour. Moisture content of raw soybean flour was higher than roasted soybean flour, and crude fat, crude protein and carbohydrate content were higher in imported soybean flour. Ash and crude protein contents of mung bean were slightly higher than imported products. Water binding capacity of roasted soybean flour in domestic products was higher than raw soy flour, and there was no significant difference in solubility and swelling power. Correlation between quality characteristics of commercially available soybean and mung-bean flours products was found to be highly significant among measured items, except for solubility.

선녹콩 개체간 및 개체내 단백질 함량 변이

임무혁,정명근 한국작물학회 2008 Korean journal of crop science Vol.53 No.-

Soybean [Glycine max (L.)] is a major source of protein for human and animal feed. Inter- and intragenotype variation of soybean protein has been investigated by soybean researchers. However, limited sample amount of soybean single seed there is no report that investigated intra-plant variation of soybean protein within soybean plant. Recently a non-destructive NIR (near-infrared reflectance) spectroscopy using single seed grain to analyze seed protein was developed. The objectives of this study were to understand variation of seed protein content within plant and to determine the amount of minimum sample size which can represent protein content for a soybean plant. Frequency distribution of protein content within plant showed normal distribution. There was an intra-cultivar variation for protein content in soybean cultivar Seonnogkong. Difference of protein content among single plants of Seonnokong was recognized at 5% level. Seeds in lower position on plant stem tended to accumulate more protein than in higher position. There was significant difference for protein content between sample size 5 seeds and sample size of more than 5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant with 57 seeds however no difference was recognized among sample size (5, 10, 20, and 30 seeds) at a soybean plant with 33 seeds. Around 20% seeds of soybean from single plant needed to determine the protein content to represent protein content of single soybean plant. This study is the first one to report evidence of intra-plant variation for protein content which detected by non-destructive NIR spectroscopy using single seed grain in soybean. Soybean [Glycine max (L.)] is a major source of protein for human and animal feed. Inter- and intragenotype variation of soybean protein has been investigated by soybean researchers. However, limited sample amount of soybean single seed there is no report that investigated intra-plant variation of soybean protein within soybean plant. Recently a non-destructive NIR (near-infrared reflectance) spectroscopy using single seed grain to analyze seed protein was developed. The objectives of this study were to understand variation of seed protein content within plant and to determine the amount of minimum sample size which can represent protein content for a soybean plant. Frequency distribution of protein content within plant showed normal distribution. There was an intra-cultivar variation for protein content in soybean cultivar Seonnogkong. Difference of protein content among single plants of Seonnokong was recognized at 5% level. Seeds in lower position on plant stem tended to accumulate more protein than in higher position. There was significant difference for protein content between sample size 5 seeds and sample size of more than 5 seeds (10, 20, 30, 40, and 50 seeds) at a soybean plant with 57 seeds however no difference was recognized among sample size (5, 10, 20, and 30 seeds) at a soybean plant with 33 seeds. Around 20% seeds of soybean from single plant needed to determine the protein content to represent protein content of single soybean plant. This study is the first one to report evidence of intra-plant variation for protein content which detected by non-destructive NIR spectroscopy using single seed grain in soybean.

Analysis of Isoflavone, Phenolic, Soyasapogenol, and Tocopherol Compounds in Soybean [Glycine max (L.) Merrill] Germplasms of Different Seed Weights and Origins

Kim, Eun-Hye,Ro, Hee-Myong,Kim, Sun-Lim,Kim, Hong-Sig,Chung, Ill-Min American Chemical Society 2012 Journal of agricultural and food chemistry Vol.60 No.23

<P>This study investigated the functional compounds, including isoflavones, phenolics, soyasapogenols, and tocopherols, that were detected in 204 soybean [Glycine max (L.) Merrill] germplasms. The soybean samples were divided into three groups according to origin: America, China, and Korea. The soybean samples were also classified into three groups on the basis of 100-seed weight: small (<13 g), medium (13–24 g), and large (>24 g). Among the soybean germplasms, CSRV121 (Bosukkong) had the highest level of isoflavone content (4778.1 μg g<SUP>–1</SUP>), whereas CS01316 had the lowest isoflavone content (682.4 μg g<SUP>–1</SUP>). Of the soybeans from the three different countries of origin, those from Korea showed the highest average concentration of total isoflavones (2252.6 μg g<SUP>–1</SUP>). The small seeds had the highest average total isoflavone concentration (2520.0 μg g<SUP>–1</SUP>) of the three different seed sizes. Among the 204 soybean germplasms, CS01405 had the highest content of total phenolics (5219.6 μg g<SUP>–1</SUP>), and CSRV017 (Hwangkeumkong) had the lowest phenolic content (654.6 μg g<SUP>–1</SUP>). The mean concentrations of total phenolic compounds were 2729.1 μg g<SUP>–1</SUP> in American soybean seeds, 1680.4 μg g<SUP>–1</SUP> in Chinese soybean seeds, and 1977.6 μg g<SUP>–1</SUP> in Korean soybean seeds. Of the soybean seeds from the three different countries of origin, American soybean seeds had the highest average concentration of total phenolic compounds, and Korean varieties showed the second highest value. Small soybean seeds had the highest average content of total phenolic compounds (2241.7 μg g<SUP>–1</SUP>), whereas medium-sized (1926.8 μg g<SUP>–1</SUP>) and large (1949.9 μg g<SUP>–1</SUP>) soybeans had lower concentrations of phenolic compounds. In whole soybean germplasms, the level of total soyasapogenols was higher in CS01173 (1802.3 μg g<SUP>–1</SUP>) and CS01346 (1736.8 μg g<SUP>–1</SUP>) than in the other types of soybeans. The mean concentrations of total soyasapogenol were 1234.0 μg g<SUP>–1</SUP> in American, 1294.5 μg g<SUP>–1</SUP> in Chinese, and 1241.5 μg g<SUP>–1</SUP> in Korean soybean varieties. Chinese soybean varieties showed the highest mean concentration of total soyasapogenol, and Korean soybean seeds showed the second highest level. The medium-seed group had the highest soyasapogenol content (1269.3 μg g<SUP>–1</SUP>) of the seeds that were grouped by size. A larger amount of soyasapogenol B than soyasapogenol A was detected. In whole soybeans, CS01202 showed the highest level of total tocopherols (330.5 μg g<SUP>–1</SUP>), whereas CSRV056 (Pungsannamulkong) had the lowest content (153.3 μg g<SUP>–1</SUP>). Chinese soybeans had the highest average concentration of total tocopherols (255.1 μg g<SUP>–1</SUP>). By comparison, the medium-sized Chinese soybean group had the highest (256.1 μg g<SUP>–1</SUP>) average total tocopherol content.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2012/jafcau.2012.60.issue-23/jf300463f/production/images/medium/jf-2012-00463f_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf300463f'>ACS Electronic Supporting Info</A></P>

Allergenicity Change of Soybean Proteins by Thermal Treatment Methods

Hui-Gyeong Seol(설희경),Yu-Jin Ko(고유진),Eun-Jung Kim(김은정),Gyeong-Lan Lee(이경란),Do-Gyeong Kim(김도경),Jeong-Ok Lee(이정옥),Kang-Mo Ahn(안강모),Chung-Ho Ryu(류충호) 한국생명과학회 2012 생명과학회지 Vol.22 No.4

콩은 우리나라에서 과민성 알레르기를 일으키는 대표적인 식품 중의 하나로, 식품으로 섭취 시에 가열 및 발효가공을 통한 형태로 섭취한다. 이에 본 연구에서는 콩에 알레르기 반응을 일으키는 7명의 환아 혈청과 1명의 정상인 혈청을 이용하여 열처리 방법에 따른 품종별 콩(대풍, 대원, 태광)의 알레르기성에 미치는 영향을 조사하였다. 단백질을 추출하여 SDS-PAGE, immunoblotting 및 ELISA 방법을 통하여 반응성을 조사한 결과, SDS-PAGE상에서 열처리하지 않은 세가지 품종의 경우 9-76 kDa 위치에서 다양한 단백질 밴드를 보였는데 특히 9, 21, 34,52, 72 그리고 76 kDa의 단백질들은 각각 LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-conglycinin의 β-subunit, α-subunit와 α’-subunit로 주요한 콩 알레르겐으로 알려져 있다. 반면에 볶은 콩, 발효한 콩에서는 35kDa 이하로 완전히 분해되어 열처리 방법을 통해 단백의 항원성이 줄어드는 것을 확인할 수 있었다. IgE immunoblotting 을 통한 세 가지 품종의 볶은 콩과 콩 알레르기 환아 혈청과의 반응에서는 공통적으로 38-40kDa과 10-15 kDa에서 단백질 밴드를 보였으나 발효한 콩에서는 대부분 반응성이 약하거나 나타나지 않았다. ELISA 결과, immunoblotting 분석과 동일하게 대부분의 환아 혈청과 반응시에 볶은 콩과 발효한 콩에서 비교적 낮은 수치를 보였다. 결론적으로 콩에 존재하는 알레르겐 단백질은 열처리와 발효 미생물이 분비하는 단백질 분해효소에 의해 대부분의 환아에서 콩 단백질과의 반응성이 약화되는 것으로 사료된다. Soybean is one of the most common food materials causing food hypersensitivity reactions in Korea. In this study, we have investigated the effect of roasting and fermentation on the allergenicity of soybean. Three kinds of soybean (Daepung, Daewon, and Taegwang) were prepared as raw, roasted, and fermented by Bacillus subtilis GSK 3580, and then their proteins were extracted. The proteins were separated using SDS-PAGE, and the detection of IgE specific to soybean proteins was performed by immunoblotting using 7 sera of soybean allergy patients and non-allergic control individuals. Serum specific IgE to soybean was measured by ELISA. The SDS-PAGE of raw soybean proteins showed various-sized bands ranging from 9 to 76 kDa, which are known as major allergens. In particular, 9, 21, 34, 52, 72, and 76 kDa proteins are known as LTP, Kunits trypsin inhibitor, Gly m Bd 30K, β-subunit, α-subunit, and α’-subunit of β-conglycinin, respectively; these are major allergens in soybean. In contrast, only peptides of less than 35 kDa were found in roasted and fermented soybeans. IgE immunoblot analysis of three roasted species of soybeans commonly detected at 38-40 kDa and 10-15 kDa. The protein bands in fermented soybean showed very weak signals or were not detected. In addition, the reactivity of most patients’ sera to soybean was decreased after roasting and fermentation. With these results, it may be concluded that the allergenicity of soybeans is reduced by the roasting and fermentation processes. It is supposed that allergenic proteins in soybean were degraded by heat treatment methods and proteolytic enzymes were secreted from fermenting microorganisms.

Health Benefits of Soybean-Prevention of Chronic Degenerative Diseases

( Jung In Kim ) 한국콩연구회 2011 韓國콩硏究會誌 Vol.28 No.1

Soybean (Glycine max.) is a good source of high quality protein, lipid with high levels of unsaturated fatty acids, oligosaccharides, dietary fiber, and isoflavones. Soybean and soy foods have received considerable attention for their potential role in decreasing risk factors for chronic degenerative diseases. This article reviews the benefits of soybean in cardiovascular diseases (CVD), cancer, diabetes mellitus, and osteoporosis and discusses the possible mechanisms. Soybean is a species of legume native to East Asia. It is consumed as soybean itself, unfermented soy products such as soy oil, soy milk, soybean curd, and textured vegetable protein (TVP), and fermented soy products such as soy sauce, fermented soybean paste, natto, and tempeh. Asians consume an average of 20 to 80 g of traditional soy foods daily, Soybean containsall three of the important macronutrients-protein, carbohydrates and fat-as well as vitamins, minerals, and dietary fiber (Cederroth and Nef, 2009). Soybean also containsan array of biologically active compounds called phytochemicals including isoflavones, saponins, and phytates. The health benefits of soybeans have become increasingly recognized worldwide. Numerous studies have reported that soybean consumption could be related with a reduced risk for cardiovascular diseases (CVD), diabetes mellitus, certain cancers, and osteoporosis (Omoni and Aluko, 2005). The aim of this review is to discuss the possible mechanisms of prevention of these chronic degenerative diseases by the bioactive components in soybean.

콩 품질평가 현황과 전망

김용호 韓國作物學會 2002 한국작물학회지 Vol.47 No.S

Soybean is one of the most important sources of protein and oil in the world. Recently, emphasis has been laid on the chemical composition of soybean seeds for the processing soybean foods. Improvement of soybean components has been expected to improve food-processing quality for the processed soybean products such as soymilk and various edible ingredients as well as fur the traditional soyfoods. In Korea, soybean breeding research programmes have been focused on the quality of the products derived from soybean with yield stability, and some new modified soybean varieties haying good food-processing quality were developed recently. So the efforts of establishing standard and standardization of products in soybean are important. Three main categories should be considered in view of soybean seed quality; the marketing value such as grain size, shape, and appearance; the eating and processing value such as dehulled ratio, water absorption rate, and benny flavor; the nutritional value such as protein, lipid, and carbohydrate contents. And the new frontiers in research are looking at the functional nutrients in soybeans and how to improve them. In case marketing value, mainly the appearance is evaluated, therefore, each country has an application of standard related to quality. Each determination of standard class, heat-damaged kernels, splits, and soybeans of other colors is made on the basis of the grain when free from foreign materials. But processing value and nutritional value for standardization were not studied in detail till now. In addition, soybean has potential roles in the prevention and treatment of chronic diseases, most notably cancer, osteoporosis, and heart disease. The functional nutrients include a protease inhibitor, phytic acid, saponins, and isoflavones, etc.. It is believed that standardization of soybean quality should perform to overcome the difficulties, relatively high price of domestic soybean products has weakened the competitive power, in the market related to WTO. So, we should focus on further research into the evaluation and establishment of quality-standard in soybean.

De novo Genome Assembly and Single Nucleotide Variations for Soybean Mosaic Virus Using Soybean Seed Transcriptome Data

Jo, Yeonhwa,Choi, Hoseong,Bae, Miah,Kim, Sang-Min,Kim, Sun-Lim,Lee, Bong Choon,Cho, Won Kyong,Kim, Kook-Hyung The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.5

Soybean is the most important legume crop in the world. Several diseases in soybean lead to serious yield losses in major soybean-producing countries. Moreover, soybean can be infected by diverse viruses. Recently, we carried out a large-scale screening to identify viruses infecting soybean using available soybean transcriptome data. Of the screened transcriptomes, a soybean transcriptome for soybean seed development analysis contains several virus-associated sequences. In this study, we identified five viruses, including soybean mosaic virus (SMV), infecting soybean by de novo transcriptome assembly followed by blast search. We assembled a nearly complete consensus genome sequence of SMV China using transcriptome data. Based on phylogenetic analysis, the consensus genome sequence of SMV China was closely related to SMV isolates from South Korea. We examined single nucleotide variations (SNVs) for SMVs in the soybean seed transcriptome revealing 780 SNVs, which were evenly distributed on the SMV genome. Four SNVs, C-U, U-C, A-G, and G-A, were frequently identified. This result demonstrated the quasispecies variation of the SMV genome. Taken together, this study carried out bioinformatics analyses to identify viruses using soybean transcriptome data. In addition, we demonstrated the application of soybean transcriptome data for virus genome assembly and SNV analysis.

Isoflavone Content and its Relationship with Other Seed Quality Traits of Soybean Cultivars Collected in South Korea

Kim, Sun-Lim,Chi, Hee-Youn,Kim, Jung-Tae,Lee, Yeong-Ho,Park, Nam-Kyu,Son, Jong-Rok,Kim, Si-Ju The Korean Society of Crop Science 2006 Korean journal of crop science Vol.51 No.1

The 117 soybean cultivars were collected from nine provinces in Korea, and various seed quality traits along with isoflavone contents were evaluated to elucidate their relationship. The 100-seed weight of the black soybean (31.2 g) was significantly higher (p<0.05) than yellow soybeans (28.6 g). The composition of genistein, daidzein, and glycitein accounted for 75.8, 22.8, and 1.4 % of total isoflavone in yellow soybean cultivars, while their compositions in black soybeans were 58.5, 39.7, and 1.8%, respectively. The mean contents of total isoflavone in yellow and black soybean were $l,561.6{\mu}g\;g^{-1}\;and\;l,018.3{\mu}g\;g^{-1}$. The isofalvone content showed significant variation among cultivars when classified by the seed size. In the yellow soybeans, total isoflavone content was higher in small size soybean cultivars $(1,776.0{\mu}g\;g^{-1})$ and medium size soybean cultivars $(1,714.3{\mu}g\;g^{-1})$ compared to large size ones $(1,518.5{\mu}g\;g^{-1})$. Genistein content was proved as the major factor determining the relationship between isoflavone content and 100-seed weights (r =-0.206*). Daidzein and glycitein, however, showed no significant relationship with the 100-seed weights. Isoflavone content was not significantly correlated with color parameters L (lightness) and a (redness) values, but color parameter b (yellowness) was positively correlated with glycitein (r=0.264*) in the yellow soybeans, while its negative correlation between daidzein (r=-0.245*) and total isoflavone (r=-0.256*) were observed in black soybeans. However, these findings suggested that the seed color value may not serve as an effective parameter for estimating the isoflavone intensity of the soybeans. Variation of protein and lipid contents between yellow soybeans (n=58) and black soybeans (n=59) was relatively stable, however, protein and lipid contents have no significant relationship with isoflavone content.

발효 과정에 따른 대두의 알레르기성 분석

정영희,권정현,정진아,이정옥,이광신,김지영,안강모,이상일,류충호 대한 소아알레르기 호흡기학회 2008 Allergy Asthma & Respiratory Disease Vol.18 No.1

Purpose:The aim of this study was to determine the allergenicity of soybean by fermentation. Methods:Non-fermented soybean, fermented soybean by Bacillus subtilis KFCC 11293 and 3-step fermented soybean by Lactococcus lactis subsp. lactis IFO 12007 and Aspergillus oryzae, B. subtilis KFCC 11293 were extracted with phosphate buffered saline (PBS). In order to detect soybean-specific IgE, we performed SDS-PAGE and IgE immunoblot analysis by using 3 kinds of soybean extracts and sera from 9 patients with atopic dermatitis. All patients were sensitive to soybean, which were confirmed by CAP-FEIA. Results:SDS-PAGE of non-fermented soybeans showed many bands, whereas only peptides of less than 15 kDa were found in fermented soybeans. IgE immunoblot analysis of fermented soybeans failed to detect specific IgE which were seen in non-fermented soybeans. Conclusion:Fermentation could reduce the allergenicity of soybeans by efficiently degrading antigenic proteins in soybeans. However, there was no significant difference between fermentation by B. subtilis and the 3-step fermentation. Purpose:The aim of this study was to determine the allergenicity of soybean by fermentation. Methods:Non-fermented soybean, fermented soybean by Bacillus subtilis KFCC 11293 and 3-step fermented soybean by Lactococcus lactis subsp. lactis IFO 12007 and Aspergillus oryzae, B. subtilis KFCC 11293 were extracted with phosphate buffered saline (PBS). In order to detect soybean-specific IgE, we performed SDS-PAGE and IgE immunoblot analysis by using 3 kinds of soybean extracts and sera from 9 patients with atopic dermatitis. All patients were sensitive to soybean, which were confirmed by CAP-FEIA. Results:SDS-PAGE of non-fermented soybeans showed many bands, whereas only peptides of less than 15 kDa were found in fermented soybeans. IgE immunoblot analysis of fermented soybeans failed to detect specific IgE which were seen in non-fermented soybeans. Conclusion:Fermentation could reduce the allergenicity of soybeans by efficiently degrading antigenic proteins in soybeans. However, there was no significant difference between fermentation by B. subtilis and the 3-step fermentation.

Isoflavone Content and its Relationship with Other Seed Quality Traits of Soybean Cultivars Collected in South Korea

Sun-Lim Kim,Hee-Youn Chi,Jung-Tae Kim,Yeong-Ho Lee,Nam-Kyu Park,Jong-Rok Son,Si-Ju Kim 韓國作物學會 2006 Korean journal of crop science Vol.51 No.1

The 117 soybean cultivars were collected from nine provinces in Korea, and various seed quality traits along with isoflavone contents were evaluated to elucidate their relationship. The 100-seed weight of the black soybean (31.2 g) was significantly higher (p<0.05) than yellow soybeans (28.6 g). The composition of genistein, daidzein, and glycitein accounted for 75.8, 22.8, and 1.4 % of total isoflavone in yellow soybean cultivars, while their compositions in black soybeans were 58.5, 39.7, and 1.8%, respectively. The mean contents of total isoflavone in yellow and black soybean were l,561.6~mug~;g-1~;and~;l,018.3~mug~;g-1 . The isofalvone content showed significant variation among cultivars when classified by the seed size. In the yellow soybeans, total isoflavone content was higher in small size soybean cultivars (1,776.0~mug~;g-1) and medium size soybean cultivars (1,714.3~mug~;g-1) compared to large size ones (1,518.5~mug~;g-1) . Genistein content was proved as the major factor determining the relationship between isoflavone content and 100-seed weights (r =-0.206*). Daidzein and glycitein, however, showed no significant relationship with the 100-seed weights. Isoflavone content was not significantly correlated with color parameters L (lightness) and a (redness) values, but color parameter b (yellowness) was positively correlated with glycitein (r=0.264*) in the yellow soybeans, while its negative correlation between daidzein (r=-0.245*) and total isoflavone (r=-0.256*) were observed in black soybeans. However, these findings suggested that the seed color value may not serve as an effective parameter for estimating the isoflavone intensity of the soybeans. Variation of protein and lipid contents between yellow soybeans (n=58) and black soybeans (n=59) was relatively stable, however, protein and lipid contents have no significant relationship with isoflavone content.