Identification of QTLs Associated with Resistance to Riptortus clavatus Thunberg (Heteroptera: Alydidae) in Soybean (Glycine max L. Merr.)

Li, Wenxin,Van, Kyujung,Zheng, Da-Hao,Liu, Weixian,Lee, Yeong-Ho,Lee, Sue-Yeon,Lee, Joon-Ho,Lee, Suk-Ha The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.4

The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. But the information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatus resistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed from the cross PI 171451 ${\times}$ Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering 1073.9 cM on 20 linkage groups (LGs). With 126 $F_5$ RILs, two independent QTLs for resistance to R. clavatus were mapped on LGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escape response to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance to R. clavatus detected in the RIL population were not significantly affected by epistatic interactions.

Identification of QTLs Associated with Resistance to Riptortus clavatus Thunberg (Heteroptera: Alydidae) in Soybean (Glycine max L. Merr.)

Wenxin Li,반규정,Da-Hao Zheng,Weixian Liu,이영호,Sue Yeon Lee,이준호,Suk-Ha Lee 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.4

The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. Butthe information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatusresistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed fromthe cross PI 171451 × Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering1073.9 cM on 20 linkage groups (LGs). With 126 F5 RILs, two independent QTLs for resistance to R. clavatus were mapped onLGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escaperesponse to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance toR. clavatus detected in the RIL population were not significantly affected by epistatic interactions.

A New Pheromone-Baited trap for Riptortus clavatus

Junheon Kim,Min-Ju Lee,Seong-Ho Kang 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10

Riptortus clavatus is one of the most serious pests affecting the cultivation of sweet persimmon, which is one of the most important export fruits in Korea. A pheromone-baited trap has been used in attempts to control this pest. Although the trap showed relatively good trap efficacy, it was often observed that R. clavatus walks around and rests on the surface of the trap rather than entering the trap. In the present study, the development of a more efficient pheromone-baited trap for R. clavatus was pursued by investigating 3 different traps: the fish trap, the pyramidal trap, and the symmetry trap. The fish trap captured the lowest number of R. clavatus, and the pyramidal trap showed moderate capture efficacy. Significantly greater numbers of R. clavatus adults were captured in the symmetry trap. Significantly increased trapping efficacy of the symmetry trap would be useful for mass trapping of R. clavatus.

콩 톱다리개미허리노린재 피해와 관련형질의 품종간 차이

오영진,조상균,김경호,백채훈,조영구,김홍식,김태수 한국육종학회 2009 한국육종학회지 Vol.41 No.4

자연발생포장 및 톱다리개미허리노린재 접종구에서 협 피해 및 수량 감소가 적었던 품종은 풍산나물콩, 소백나물콩, 두유콩, 신팔달콩 2호로 다른 품종에 비해 노린재 선호도가 낮은 품종으로 생각되었고, 명주나물콩, 만리콩, 황금콩은 피해가 심한 품종으로 분류되었다. 콩 품종간 톱다리개미허리노린재 선호성으로 모용색이 회백색인 품종군의 피해율이 13.0%인데 비해 갈색모용의 품종군은 8.2%로 피해율이 낮았으며, 제색도 황색에 비해 담갈색>갈색>회갈색 순으로 제 In recent years, an increasing amount of research has been conducted on the yield loss of soybean crop caused by Riptortus clavatus, a serious pest of legumes. The objective of this study were to examine crop damage caused by various population densities of R. clavatus and determine the association of unique growth and seed characteristics with crop damage rate in conventional soybean fields. Major growth responses to R. clavatus were examined. The cultivars that were not as seriously damaged by R. clavatus were Pungsannamulkong, Sobaegknamulkong, Duyoukong, and Sinpaldal 2, but Myeongjunamulkong, Mallikong, and Hwangkeumkong were significantly (P<0.05) damaged. The rate of seed damage caused by R. clavatus was 8.2% in a group of brown colored pubescence, while the rate was 13.0% in a group of the gray colored. Crop damage by R. clavatus clearly more reduced in cultivars with dark hilum colors including dark brown, brown, and grayish brown than those with yellow hilum colors. Concerning pod colors, crop damage was less significant in cultivars with dark brown pod color than with the light brown. Cultivars with short pod length (4.0-4.5 cm) at full seed (R6) stage showed lower seed damage rates by R. clavatus than those with long pod length (>5.6 cm). Therefore, characteristics of hilum, pod, pubescence colors, and pod length were significantly related and linked to the cultivar resistance to R. clavatus.

Repellent Activity and Electroantennograph of Caraway and Clove Bud Oil against Bean Bug Riptortus clavatus (Thunberg) (Hemiptera: Alydidae)

Jeong-Oh Yang,Yun-Hee Kwon,Changmann Yoon,Gil-Hah Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Thirteen plant essential oils were tested for their repellent activity against the bean bug Riptortus clavatus. Among the tested oils, caraway (100%) and clove bud oil (92%) significantly repelled the bean bugs at a dose of 0.142㎕/cm2 by using a Y-tube olfactometer. GC and GC-MS analyses revealed that the active components responsible for the effective repellency of caraway and clove bud oil were carvone (75%) and limonene (76.9%); eugenol (100%), isoeugenol (54.3%) and β-caryophyllene (60.0%), respectively. Of the different active fractions, eugenol was the most significant one than the other components with reference to repellent activity against the bean bugs. In the GC-EAD, limonene and carvone of caraway oil were responded to the antenna of Riptortus clavatus.

Fish Materials as Potent Attractants for the Male of Bean Bug, Riptortus clavatus

Huh, Wan,Huh, Hye-Soon,Park, Chung-Gyoo Korean Society of Applied Entomology 2005 Journal of Asia-Pacific Entomology Vol.8 No.4

Fish materials were tested for their attractiveness to the bean bug, Riptortus clavatus (Thunberg) (Heteroptera: Alydidae) in a soybean field. Fish materials' volatiles evoked male-specific responses in attractiveness. Fish materials such as mackerel, hairtail, and gizzard shad attracted significantly higher number of males than control, but they were not attractive to females. The attractiveness of MeOH extract of mackerel to R. clavatus males was not different from 10 live males and aggregation pheromone traps of R. clavatus. Further studies are needed to identify volatile compound(5) from fish smell responsible for the attraction of R. clavatus males.

Repellency and Electrophysiological Response of Caraway and Clove Bud Oils against Bean Bug Riptortus clavatus

( Jeong Oh Yang ),( Ji Hyun Park ),( Bong Ki Son ),( Sang Rae Moon ),( Shin Ho Kang ),( Chang Mann Yoon ),( Gil Hah Kim ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6

This study was performed to investigate the repellent activity of thirteen plant essential oils and to confirm their electrophysiological response against the bean bug Riptortus clavatus. Among the tested oils, caraway (100%) and clove bud oil (92%) significantly repelled the bean bugs at a dose of 0.142 μL/cm2 by using an olfactometer. GC-MS analysis revealed that the active components responsible for the effective repellency of caraway and clove bud oil were carvone (56%) and limonene (44%); eugenol (81%), isoeugenol (12%) and β-caryophyllene (7%), respectively. Of the different active constitutions, eugenol was the most significant one than the others with reference to repellent activity against the bean bugs. Analysis by GC-EAD showed, major components of two essential oils that elicited responses in R. clavatus antennae, indicating the potential role of the essential oils as repellents that determine the choice of the repellent material.

Survivorship of the bean bug, Riptortus clavatus, fed on seed extract fractions of amungbean variety

Jin Kyo Jung,Bo-Yoon Seo,Jung-Kyung Moon,Ja-Hwan Ku 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

To isolate plant insecticidal factors, a series of survivorship tests were conducted using artificial diets mixed with seed extract of a mungbean variety (Vigna radiata), which gave a high mortality to the bean bug, Riptortus clavatus. Seed flour was extracted with methanol and ethanol solutions, and then the crude extract was first fractionated with layers of ether, n-butanol and water in solvent/solvent partition. Layers of ether and n-butanol fractions showed insecticidal effects to the bean bug, respectively. The n-butanol fraction was further isolated with several fractions on a reversed-phase column chromatography, and its 100% methanol fraction showed a high insecticidal effect. The fraction was further isolated on a normal-phase column chromatography, and the 70% methanol fraction showed the highest insecticidal effect. The final fraction was analyzed and fractionated on a reversed-phase HPLC. The active isolates were almost insoluble in any organic solvents and did not show any absorption in ultra-violet and visible light wavelength range. On the other hand, the ether fraction of crude extract was isolated on a normal phase column chromatography, and its hexane fraction showed the activity. The hexane fraction was further isolated on a reversed-phase HPLC and tested for the survivorship of the bean bug.

Repellency and Electrophysiological Response of Caraway and Clove Bud Oils against Bean Bug Riptortus clavatus

Jeong-Oh Yang,Ji-Hyun Park,,Shin-Ho Kang,Changmann Yoon,김길하 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6

This study was performed to investigate the repellent activity of thirteen plant essential oils and to confirm their electrophysiological response against the bean bug Riptortus clavatus. Among the tested oils, caraway (100%) and clove bud oil (92%) significantly repelled the bean bugs at a dose of 0.142 µL/cm by using an olfactometer. GC-MS analysis revealed that the active components responsible for the effective repellency of caraway and clove bud oil were carvone (56%) and limonene (44%); eugenol (81%), isoeugenol (12%) and β-caryophyllene (7%), respectively. Of the different active constitutions, eugenol was the most significant one than the others with reference to repellent activity against the bean bugs. Analysis by GC-EAD showed, major components of two essential oils that elicited responses in R. clavatus antennae, indicating the potential role of the essential oils as repellents that determine the choice of the repellent material.

Repellency and Electrophysiological Response of Caraway and Clove Bud Oils against Bean Bug Riptortus clavatus

Yang, Jeong-Oh,Park, Ji-Hyun,Son, Bong-Ki,Moon, Sang-Rae,Kang, Shin-Ho,Yoon, Chang-Mann,Kim, Gil-Hah The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6

This study was performed to investigate the repellent activity of thirteen plant essential oils and to confirm their electrophysiological response against the bean bug Riptortus clavatus. Among the tested oils, caraway (100%) and clove bud oil (92%) significantly repelled the bean bugs at a dose of 0.142 ${\mu}L/cm^2$ by using an olfactometer. GC-MS analysis revealed that the active components responsible for the effective repellency of caraway and clove bud oil were carvone (56%) and limonene (44%); eugenol (81%), isoeugenol (12%) and ${\beta}$-caryophyllene (7%), respectively. Of the different active constitutions, eugenol was the most significant one than the others with reference to repellent activity against the bean bugs. Analysis by GC-EAD showed, major components of two essential oils that elicited responses in R. clavatus antennae, indicating the potential role of the essential oils as repellents that determine the choice of the repellent material.