Recombinant Bovine Somatotropin의 피하주사 후 Male과 Female Rat에서 약물동태 및 조직분포

박병권,박승춘,윤효인,Park, Byung-kwon,Park, Seung-chun,Yun, Hyo-in 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

The purpose of this study is to investigate the pharmacokinetics and tissue distribution of recombinant bovine somatotropin (rBST) after subcutaneous adminstration of $^{125}I-rBST$ in male and female rats. A solid state conjugation (Iodo-bead$^{(R)}$) method was confirmed useful for producing $^{125}I-rBST$ because the administration of the conjugated form enabled enough to determine time- concentration relationships of rBST in rats. Subcuatenous administrations showed sex differences that female ($t_{1/2,kc}$, 2.87 h) revealed rapid elimination as compared to male ($t_{1/2,ke}$, 4.81 h), with the absorption ($t_{1/2,ka}$ in male being 0.3 h and that in female 0.75 h) in the reverse order. For subcutaneous administration of rBST in male rats, the liver was the highest in amount, followed by kidney, testes, muscle, and stomach, at the slaughtering tame of 1, 6, 12 and 24 h. But the testes was the highest at the 48 h slaughtered animals, followed by liver, kidney, stomach, and muscle. In slaughtered females at 1, 6, and 12 h after the administration of rBST, the liver was the highest, followed by ovary, kidney, small intestine, and stomach. At 24 and 48 h slaughtered female rats, the ovary was the highest, the liver the second, and the kidney the third.

Recombinant Bovine Somatotropin의 피하주사 후 Male과 Female Rat에서 약물동태 및 조직분포

박병권(Byung Kwon Park),박승춘(Seung Chun Park),윤효인(Hyo In Yun) 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

Effect of Recombinant Bovine Somatotropin (rBST) Administration on Residual BST and Insulin-like Growth Factor-I Levels in Various Tissues of Cattle

CHOI, Jeongeun,CHOI, Myung Ja,KIM, Choonmi,HA, Jongkyu,HONG, Byungju,JI, Yongtae,CHANG, Byungsun 梨花女子大學校 藥學硏究所 1998 藥學硏究論文集 Vol.- No.7

This study was performed to investigate the effects of recombinant bovine somatotropin(rBST)administration on residual BST and insulin-like growth factor-Ⅰ (IGF-Ⅰ)levels in various tissues. Two experiments, (A) and (B) were conducted as follows. (A) Beef cattle (average b.w. of 450 ㎏) were divided into three groups: control (CONT), low-dose (LOW) and high-dose(HIGH) groups. The CONT group received neither rBST nor vehicle. The LOW and HIGH groups were treated for 20 weeks by s.c. injection with 250 ㎎ rBST at one-week intervals and 500 ㎎ rBST at two-week intervals, respectively. (B) Beef cattle were divided into four groups : control (CONT) group, as described in experiment (A), and sustained-release, low-dose (SR-L),sustained-release, medium-dose (SR-M) and sustained-release, high-dose (SR-H) groups. SR-L, SR-M and SR-H were treated at two-week intervals for 24 weeks by s.c. injection with doses of 0.42 ㎎ rBST/㎏ b.w. (0.03 ㎎ rBST/㎏ b.w./day), 0.84 ㎎ rBST/㎏ b.w. (0.06 ㎎ rBST/kg b.w./day) and 1.26 ㎎ rBST/㎏ b.w. (0.09 ㎎ rBST/㎏ b.w./day), respectively. In both experiments, animals were killed two weeks after the final treatment. In experiment (A), residual BST and IGF-Ⅰ levels in muscle tissues of the LOW and HIGH groups were not affected by rBST administration. In experiment (B), residual BST and IGF-Ⅰ levels in muscle, fat, liver and kidney tissues of treated groups were not different from that of the CONT group, either. These results suggest that levels of BST and IGF-Ⅰ in various tissues are not increased at two weeks after rBST administration.

유전자재조합 Bovine Somatotropin 투여가 우유 중의 Bovine Somatotropin 농도에 미치는 영향

최정은,최명자,진재호,김주호,박종세 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.2

The present study was conducted to examine the effect of recombinant bovine somatotropin(γBST), which was administered to cow to promote milk production, on bST levels in milk. Fourteen cows were divided into two groups: 1) control cows received neither γbST nor vehicle, 2) treated cows were administered twice at two-week interval with 500 mg γbST each cow by subcutaneous injection. Milk samples were taken on day 0 (prior to injection), day 7 (7 days after 1st injection), day 21 (7 days after 2nd injection) and day 35 (21 days after 2nd injection). Milk bST concentration was measured by the radioimmunoassay method. There was no statistical difference(p$lt;0.05) in milk bST levels between two groups showing bST levels in the range of 1.8 ng/ml to 3.1 ng/ml. That is, γbST administration did not increase bST levels in milk.

소 재조합 somatotropin 단백질을 이용한 한우 유량 증진 효과

우제석(Jae-Seok Woo),이승환(Seung Hwan Lee),이명식(Myeung Sik Lee),박정용(Jung Yong Park),김민규(Minkyu Kim),홍성구(Sunggoo Hong) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.4

This study was carried out to increase the breeding rate using sustained recombinant bovine somatotropin (bST) and examine the reproductive disorder and disease prevalencies of cow and calf, respectively. Sixty cows were allotted randomly to three groups in randomized complete block design; (1) No treatment, (2) 500 mg bST two times + 250 mg bST SC injection five times from 3 weeks before to 9 weeks after parturition every two weeks, (3) 500 mg bST two times before 3 weeks parturition + 250 mg bST SC injection three times from five to 9 weeks after parturition every two weeks. Developmental data of birth weight, weaning weight, total gain and average daily gain in control was 22.9±3.45 kg, 88.0±11.13 kg, 65.1±9.74 kg and 0.54±0.08 kg, respectively. There is no significant difference between Group 1 and 3 in the examined factors. However, there is significant difference between Group 1 and 2 in the birth weight (24.4±2.88 kg), weaning weight (101.0±11.77 kg), total gain (76.7±7.9 kg) and average daily gain (76.7±7.9 kg). Total gain was showed higher in Group 2 and 3 than Group 1 in the >2nd parity, and in Group 2 than Group 1 and 3 in the 1st parity. There is no significant difference in the BCS, no return days and disease occurrence of cow and calf by bST treatment. Number of service per conception was slightly increased in the Group 3 compared to Group 1 and 2. To increase the breeding ability of Hanwoo, it is recommended that 500 mg bST two times + 250 mg bST injection five times from 3 weeks before to 9 weeks after parturition every two weeks.

Comparative Efficacy Evaluation of Different Administration Routes on a Recombinant Bovine Somatotropin in Flounder (Paralichthys olivaceus)

Ki-young Yoon 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.1

The administration routes of a recombinant bovine somatotropin (rBST) were compared for their growth-promoting ability in flounder (Paralichthys olivaceus). In a 15-day short-term investigation, the efficacy of intramuscular (20 ㎎/㎏, once) injection was higher than that of oral (20 ㎎/㎏, weekly) administration in diet, although significantly-high efficacies were not obtained by both administration routes. In 10-week long-term evaluation, however, significant growth promoting effects were achieved with intramuscular (20 ㎎/㎏, biweekly) or intraperitoneal (20 ㎎/㎏, biweekly) administrations, in which intramuscular administration was superior to intraperitoneal route. Taken together, it is suggested that a long-term administration via parenteral route is required for the effective therapy of rBST in flounder.

소 재조합 somatotropin 단백질을 이용한 한우 유량 증진 효과

우제석(Jae-Seok Woo),이승환(Seung Hwan Lee),이명식(Myeung Sik Lee),박정용(Jung Yong Park),김민규(Minkyu Kim),홍성구(Sunggoo Hong) 충남대학교 농업과학연구소 2011 Korean Journal of Agricultural Science Vol.38 No.4

This study was carried out to increase the breeding rate using sustained recombinant bovine somatotropin (bST) and examine the reproductive disorder and disease prevalencies of cow and calf, respectively. Sixty cows were allotted randomly to three groups in randomized complete block design; (1) No treatment, (2) 500 mg bST two times + 250 mg bST SC injection five times from 3 weeks before to 9 weeks after parturition every two weeks, (3) 500 mg bST two times before 3 weeks parturition + 250 mg bST SC injection three times from five to 9 weeks after parturition every two weeks. Developmental data of birth weight, weaning weight, total gain and average daily gain in control was 22.9±3.45 kg, 88.0±11.13 kg, 65.1±9.74 kg and 0.54±0.08 kg, respectively. There is no significant difference between Group 1 and 3 in the examined factors. However, there is significant difference between Group 1 and 2 in the birth weight (24.4±2.88 kg), weaning weight (101.0±11.77 kg), total gain (76.7±7.9 kg) and average daily gain (76.7±7.9 kg). Total gain was showed higher in Group 2 and 3 than Group 1 in the >2nd parity, and in Group 2 than Group 1 and 3 in the 1st parity. There is no significant difference in the BCS, no return days and disease occurrence of cow and calf by bST treatment. Number of service per conception was slightly increased in the Group 3 compared to Group 1 and 2. To increase the breeding ability of Hanwoo, it is recommended that 500 mg bST two times + 250 mg bST injection five times from 3 weeks before to 9 weeks after parturition every two weeks.

Delayed Clinical Signs Associated with Cerebellar Lesion in Holstein Calf Infected with Bovine Viral Diarrhea Virus

김단일 한국임상수의학회 2022 한국임상수의학회지 Vol.39 No.1

A 5-month-old Holstein downer calf was presented, and bovine viral diarrhea virus was detected in the feces and the cerebrospinal fluid. Combined treatment of recombinant bovine somatotropin (rBST) administration and standing rehabilitation had been performed for two months, and the calf could maintain the standing position for a while. However, the symptoms were not improved, and the calf died due to ruminal tympany and aspiration pneumonia. Megaesophagus, abomasal ulcer, and a normal-sized but softened cerebellum were observed in clinical necropsy. Improvement of symptoms was confirmed by rBST, but was not reached the complete recovery of gait and standing position

Effects of Cooling and Exogenous Bovine Somatotropin on Hematological and Biochemical Parameters at Different Stages of Lactation of Crossbred Holstein Friesian Cow in the Tropics

Chaiyabutr, N.,Boonsanit, D.,Chanpongsang, S. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.2

Effects of cooling and supplemental recombinant bovine somatotropin (rbST) on hemato-biochemical characteristics were studied at different stages of lactation of crossbred Holstein Friesian cows in a tropical environment. Ten primiparous cows were divided into two groups of five animals each. The first group was housed as the non-cooled animals in an open-sided barn with a tiled roof in a normal shaded house (NS), while the second group was housed as cooled cows in an open-sided barn with a tiled roof under misty fan cooling (MFC). Three injections with rbST (500 mg per dose) at each stage of lactation (early, mid and late lactation) significantly increased total milk yield as compared with pretreatment in both cooled and non-cooled cows. Milk fat was significantly increased, while total solids, solid not fat, milk protein and lactose were not affected by the rbST treatment. Hematological parameters, plasma proteins, albumin, glucose, triglyceride, cholesterol, creatinine, alkaline phosphatase (ALP), plasma inorganic phosphate and the activities of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected by supplemental rbST in cooled and non-cooled cows. Supplementation of rbST caused a significant decrease in plasma urea concentration, while plasma FFA concentrations significantly increased in both cooled and non-cooled cows. The results of the present study suggest that exogenous rbST is efficacious in increasing milk yield without adverse effects on lactating crossbred Holstein cows in a tropical environment.

Effect of Recombinant Bovine Somatotropin on Growth of Olive Flounder, Paralichthys of olivaceus

Rho, Sum-Rho,Kim, Pil-Youn,Lee, Young-Don,Choi, Kwang-Sik,Song, Choon-Bok The Korean Society of Fisheries and Aquatic Scienc 1999 韓國養殖學會誌 Vol.12 No.2

The recombinant bovine somatotropin (rBST) was administered to the oliver flounder, Paralichthys olivaceus, to know the effects and optimal administration frequency and dosage of the hormone. The experiment was conducted with three different treatment groups (A, B and C) designated based on the duration and administration frequency of rBST and one control (D) from April 14, 1996 to March 16, 1997. The fish of hormone treated groups grew 7.86 to 10.07% (47.45 to 60.75 g in weight) better than the control at the end of the experiment (P<0.05). No significant differences in their growth were detected among treatment groups. The distinct growth improvement was recognized four weeks after completion of the first four hormone administration. When considering water temperatures measured from the experimental tanks, the effect of rBST on the flounder was greater during the period showing relatively lower temperature. The survival rates were higher in treatment groups than in the control, revealing 98.3% (A), 98.4% (B), 97.7% (C) and 93.1% (D) during the first stage of culture; 92.7% (A), 91.3% (B), 86.7% (C) and 80.0% (D) during the second stage of culture.