한국잔디 유전자원의 내마모성 지표로서 MDA함량과 형태적 특성과의 상관분석

배은지(Eun-Ji Bae),조현민(Hyun-Min Cho),윤준혁(Jun-Hyuck Yoon),진언주(Eon-ju Jin),이긍주(Geung-Joo Lee) 한국잡초학회 2021 Weed & Turfgrass Science Vol.10 No.4

본 연구는 한국잔디 수집 유전자원의 답압 처리에 따른 지상부 마모 스트레스 정도를 정량적으로 평가하기 위한 지표를 알아보고자 실시하였다. 그 중 스트레스 환경에서 지질막 구성물질의 높은 산화 정도를 정량적으로 나타낼 수 있는 MDA (malondialdehyde) 함량과 잔디 지상부 형태적 특징과의 상관관계를 분석하여 통계학적으로 유의성이 높은 지표들을 조사하였다. 전국에서 수집된 한국잔디류(Zoysia spp.) 242개체 중 높은 피복률을 보이는 적응력이 우수한 144개체를 선발하여 형태적 특징을 조사하였고, 해당 유전자원들을 답압 처리 후 마모스트레스를 주어 MDA 함량을 측정하였다. 식물체가 환경적 스트레스에 노출 시 유발되는 생리적 산화 반응물인 MDA의 축적 정도가 유전자원간에 통계적으로 유의한 차이가 있는 것을 확인하였고, 이를 이용한 MDA 함량의 통계적 차이로 서로 독립된 세 개 집단(L, M, 그리고 H)으로 분류할 수 있었다. 상관성 분석 결과, 마모 스트레스 후에도 생체 내 산화 반응 정도가 낮아 스트레스로 인한 피해 수준이 적을 것으로 보이는 낮은 MDA 함량 그룹(L)에서 포복경 마디지름이 두껍고, 엽폭이 넓고, 엽장이 길고, 초장과 최하위 잎 높이가 높으며, 잎각도가 좁게 발달하는 형태적 특징을 갖는 결과를 확인 할 수 있었다. 잎각도를 제외한 나머지 5개 형태적 특성은 MDA 함량 수준과 매우 밀접한(P<0.001) 연관이 있는 것으로 조사되었지만, 다른 연구에서와 같이 실제 내마모성 기작과 높은 관련성이 있는 줄기밀도, 신초의 탄력성, 리그린 및 리그노셀룰로오스 함량, 잎의 K 함량 등도 추가적으로 조사되어 종합적으로 분석된다면 MDA 함량 측정으로 내마모성을 어느 정도 예측할 수 있을지 판단하는데 도움이 될 것으로 여겨진다. This study was conducted to find out indicators to quantitatively evaluate the degree of wear stress on aerial shoots after traffic stress on zoysiagrass collections in South Korea to be used in the school playground. The contents of malondialdehyde (MDA) which may indicate the oxidation degree of cell membrane under stress was correlated with morphological characteristics of zoysiagrass shoots. A wear simulation roll made of aluminum cylinder at 40 kg weight and 1.1 m width with circular studded projections was used to apply artificial traffic stress of pressure and wear for 144 zoysiagrass accessions that had 70% or more of ground coverage. The equipment was driven by the motor at a steady speed rate to reciprocate in the designated potted turfgrass trays in the greenhouse. For morphological characteristics, plant height, leaf length, lowest leaf height, leaf width, leaf angle, stolon length, internode length and diameter of stolon, and stolon node number were investigated. It appeared that there was a statistically significant difference among zoysiagrass accessions in the MDA content, with grouping into 3 categories. From the correlation analysis, the low MDA content group (L) where shoot damage is expected to be relatively weak due to the low degree of stressful oxidation in vivo even after the traffic stress showed thicker node diameter, wider leaf width, longer leaf length, higher plant height and lowest leaf, and narrower leaf angle. Although significantly correlated with those morphological measurements, it was thought that the MDA content alone had a limit to be used as a wear tolerance index.

동일한 에너지 소비 시 운동 강도에 따른 총항산화력(TAC)과 과산화지질(MDA)의 변화

이광식 한국체육과학회 2007 한국체육과학회지 Vol.16 No.1

The purpose of this study is to compare the Total Antioxidant Capacity(TAC) and Malondialdehyde(MDA) between VT-20 and VT+10 of intensity exercise with the same energy expenditure. 7 male students majoring in physical education were optionally selected as the subjects. In the first experiment, the graded exercise test with Burce protocol was executed to determine the VT for each exercise. In the second experiment, the subjects were performed to walk and/or run at the speed of VT-20(6.84±1.01 km/hr, 29/29±1/19 min/sec, 50.85±5.17 %O2max) and VT+10(11.49±1.26 km/hr, 17/25±0/17 min/sec, 80.85±5.17 %O2max) was set by the same energy expenditure, respectively. TAC and MDA were measured with FRAS 4. The results are as follows. 1. Both of VT-20 and VT+10 of exercise intensity with the same energy expenditure were increased after the exercise on TAC, respectively. Moreover, there was significant increment of TAC revealed in VT+10 compared to VT-20. 2. Only in VT-20 increased after the exercise on MDA. However, there was no significant different represented between VT-20 and VT+10 on MDA. The results suggested that although do exercise of high intensity if the energy expenditure is as same as that of moderate exercise within submaximal, it may activate the TAC to inhibit the production of MDA.

고지방식이에서 서로 다른 불포화 지방산 섭취가 쥐의 혈청과 조직의 Tocopherol 함량과 지질과산화물 형성에 미치는 영향

남정혜 한국식품영양학회 1994 韓國食品營養學會誌 Vol.7 No.4

본 연구에서는 생후 17주된 Sprague Dawley종 수컷쥐에게 식이지방량이 총섭취 열량의 40%인 고지방 식이시 n6 linoleic acid, n3 EPA와 DHA 지방산을 충분한 양의 tocopherol과 한께 6주간 투여하였을 때 이들 지방산이 plasma와 RBC, 간조직의 tocopherol, MDA함량과 간의 SOD, GSH-Px의 활성에 미치는 영향을 관찰하여 다음과 같은 결론을 얻었다. Plasma tocopherol은 지방산의 불포화도가 높을수록 감소되었으나 plasma MDA는 식이 지방산 종류에 의한 차이가 없었다. RBC MDA는 n6 LA에 비해 n3 LL와 EPA + DHA에 의해 증가되었고 RBC hemolysis도 증가되었다 간과 RBC의 tocopherol 함량은 식이 지방산의 종류에 따른 영향이 없없으나 MDA함량은 유의성 있게 증가되었다. 간조직의 SOD와 GSH-Px활성은 식이 지방산의 불포화도가 높을수록 모두 증가되었으나 GSH-Px는 SOD에 비하여 식이지방산에 의한 영향이 덜하였다. 결론적으로, 고지방 식이를 할 경우 SOD와 GSH-Px 활성이 높아져도 RBC와 간조직의 MDA는 오히려 증가되어 지질 과산화물의 생성을 막아주지 못하였다. 최근 우리나라의 경우 동물성 식품의 계속적인 섭취 증가로 식이중 열량과 지방의 양이 계속 증가되고 있는 추세이며 이로인한 여러 순환기계질환의 예방을 위해 n3 PUFA를 섭취할 경우 결국 더 많은 tocopherol섭취가 필요할 것으로 사려된다. This study was observed the effect of n6 and n3 polyunsaturated fatty acids of dietary corn oil and fish oil which was supplemented with similar levels of tocopherol in high fat diet on the levels of tocopherol, malondialdehyde(MDA) productions of plasma and tissues of rats. Also RBC hemolysis, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in liver were determined. Male Sprague Dawley rats were fed high fat(40%Cal) diet which was different only in fatty acid composition for 6 weeks. Dietary fats were beef tallow(BT) as a source of saturated fatty acid, corn oil(CO) for n6 linoleic acid(LA) and fish oil(FO) for n3 eicosapentaenoic acid(EPA) + docosahexaenoic acid (DHA). Plasma and liver tocopherol levels were lowered by n3 PUFA, but there was no difference in malondialdehyde(MDA) level by different dietary PUFA. However, MDA content of RBC and hemolysis were increased by n3 PUFA. MDA content, superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px) activities in liver were increased in more unsaturated dietary fat groups. Especially, SOD activity was increased in proportion to the degree of fat unsaturation.

운동강도에 따른 12주간 유산소성 운동이 지질 과산화물(MDA)과 항산화 효소(SOD)에 미치는 영향

엄우섭 한국운동생리학 2004 운동과학 Vol.13 No.3

본 연구의 목적은 12주간 운동강도에 따른 유산소성 운동이 지질 과산화물(MDA)과 항산화 효소(SOD)에 어떠한 영향을 미치는지를 알아보고, 산화적 스트레스를 줄일 수 있는 적정 운동강도를 파악하는 것이다. 이를 위하여 건강한 일반 남자대학생(22.53±1.91세) 32명이 12주간의 유산소성 운동 전후에 최대 운동부하검사에 참여했으며, 2명은 탈락했다. 피험자는 크게 운동집단(24면)과 통제집단(8명)으로 나누었으며 운동집단은 다시 운동강도 별로 저강도(50% VO₂max), 중강도(70% VO₂max) 고강도(85% VO₂max) 운동집단으로 나누었다. 운동상황(안정시 운동직후, 회복기 1시간)에 따른 지질 과산화물(MDA)의 생성과 항산화 효소(SOD)의 활성의 변화를 알아보고자 최대운동검사를 통해 사전·사후실험을 수행하였으며, 최대운동검사는 Bruce Protocol을 이용하여 점증운동부하검사를 실시하였다. 최대운동부하검사를 통하여 최대산소섭취량, 안정시 및 최대 심박수를 측정하였으며, 그 측정치를 근거로 트fp이닝 처치를 위한 운동강도를 설정하였다. 운동강도는 사전실험을 통해 얻은 VO₂max의 50%, 70%, 85%,에서의 속도와 경사도를 산출하고 50%, 70%, 85%의 %HRR을 산출 적용하였으며, 강도별 3 운동집단은 개인별로 산출된 운동강도에 따라 300㎉에 도달할 때까지 하루 저강도(20분), 중강도(30분), 고강도(40분) 씩 주 3회 트레드밀 운동을 12주간 실시하였다. 운동처치 6주 후에 체력이 향상됨에 따라 중간실험(최대운동부하검사)을 통하여 새로운 운동강도를 설정하였으며 12주까지 그 강도로 트레이닝을 실시하였다. 채혈은 간호사에 의해서 왼쪽 전완주정맥(antecubital vein)에서 채혈시점별로 각각 10㎖ 정도의 채혈을 하여 혈액을 분석 검체에 따라 항응고 처리된 tube에 넣어 처리 하였다. 첫째, 지질 과산화물(MDA)은 집단간에 유의한 차이가 나타나지 않았으나 트레이닝 전후, 채혈시점별로는 유의한 차이가 나타났으며(P<.01, .001), 상호작용 효과는 없는 것으로 나타났다(P<.05). 둘째, 항산화 효소(SOD)는 집단간에 유의한 차이가 나타나지 않았으나 트레이닝 전후, 채혈시점별로는 유의한 차이가 나타났으며(P<.001), 트레이닝 전후와 채혈시점간에 상호작용 효과가 있는 것으로 나타났다(P<.05). 결론적으로 트레이닝은 세포내 항산화 효소를 증가시킴으로써 지질 과산화를 야기하는 정도의 운동을 예방하거나 최소화해주는 경향이 있는데, 본 연구에서는 MDA 농도가 중강도(70%HRR)에서 유의하게 증가하고, SOD 활성도는 저강도(50%HRR)에서 유의하게 증가하였다. 따라서, 20대의 일반 남성이 유산소성 운동이나 장기간 유산소성 트레이닝을 할 경우 70% VO₂max 보다 낮은 운동강도로 하는 것이 산화적 손상을 최소화할 수 있을 것으로 생각된다. The purpose of this study was to investigate the effect of 12wks of aerobic exercise with different exercise intensity on lipid peroxidation(MDA) and antioxidant Enzymes(SOD), and determine optimal exercise intensity reduced oxidative stress. Healthy college-aged males(22.53±1.91yr) were participated in the maximal exercise test before(W32) and after(N= ) 12wks of aerobic exercise. The subjects were divided into two groups; control groups(N4, training group(N=24). Then training group was divided into three subgroups; 50%HRR(low intensity) training group(N), 70 HRR(middle intensity) training group(N=8), 85%HRR(high intensity) training group(N=8). Two persons were eliminated during the training. To investigate the effect of aerobic exercise on oxidative stress(MDA. SOD) were measured at rest, immediately after exercise and 60min recovery. Maximal exercise testing) was used the Bruce protocol on the treadmil. VO₂max, were measured by maximal exercise testing, exercise intensity was determined for aerobic training treatment a basis of this measured values. speed and grade at 50%, 70%, 85%VO₂max, and 50%, 70%, 85% of %HRR gained pre test were produced and applied. The programs of exercise groups were aerobic treadmill exercise for 12wks. Three groups(Low, middle, high intensity) performed almost 40min, 30min, 20min per day(3days(week) by 30061, respectively. Venous blood were drawn at the forearm antecubital vein at rest, immediately after exercise and 60mn recovery. The concentration of plasma malondialdehyde(MDA), activities of superoxide dismutase(SOD) were analized in venous blood. First, MDA levels increased more significantly in exercise than resting values(p < 0.01) and it increased more significantly after than before training(p < 0.001). Also, significant differences were only observed at 60min recovery among the groups. Second, it was found that blood SOD concentration showed a main effect for before and after training(p < 0.001). blood sampling time(p < 0.001), before and after training and blood sampling time(p < 0.05) by three repeated ANOVA, decreased more significantly in exercise than resting values(p < 0.001). and increased more significantly after than before training(p < 0.001). In general, Physical training tends to prevent or minimize that the degree of exercise induced lipid peroxidation, possibly by increasing cellular antioxidant enzymes, but in this study MDA level increased significantly at middle intensiry(70%HRRI, activity of SOD increased significantly at low intensity(50%HRR) compared to other groups by aerobic training. Therefore, it has been suggested that low exercise intensity than 70%HRR in college-aged man protect oxidative damage from various oxidative stress produced by chronic aerobic exercise.

노인여성의 리듬운동 참여가 항산화 작용 및 혈중지질에 미치는 영향

김선영(Sun Young Kim),서재명(Jae Myeong Suh) 한국무용과학회 2008 한국무용과학회지 Vol.17 No.-

본 연구의 목적은 노인여성에게 16주간 리듬운동을 적용하여 항산화 작용과 혈중지질에 어떠한 효과가 있는지 검증하기 위한 것이며, 피험자는 H 대학교 사회교육원에서 운동프로그램에 자발적으로 참여하고자 하는 60-65세 노인여성을 24명을 대상으로 실시하였다. 이들은 의학적으로 특별한 소견을 보이지 않는 자로 하였으며, 운동프로그램 참여 전 3개월 전부터 항산화제와 관련된 약물을 섭취하지 않는 자로 선정하였다. 또한 실험기간 중에도 이와 관련된 약물 등을 제한 할 것과 운동 프로그램에 참여를 원하는 12명을 실험군으로, 동일한 조건에 운동에 참가를 원하지 않는 12명을 통제군으로 하였다. 대상자를 선정한 후 요가운동 적용 전에 총 항산화능(total antioxidant status), MDA(malondialdehyde), 혈중지질은 Total cholesterol, Triglyceride, LDL-cholesterol, HDL-cholesterol의 수준을 측정 하였으며 운동이 끝난 후 16주 뒤에 동일한 방법으로 측정하였다. 연구결과를 검증하기 위한 통계방법은 SPSSWIN version 10.0 program을 사용하였으며, 두 집단 차이는 Independent t-test를 이용하였고 각 집단의 전·후 차이는 Paired t-test 실시하여 다음과 같은 결론을 얻었다. 1. 운동 처치 후 항산화능은 두 집단간에 유의한 차이를 보였고, MDA는 운동 전·후에 유의한 차이를 보였으나, 두 집단간에는 유의한 차이를 보이지 않았다(P>.05), 2. 혈중지질은 총 콜레스테롤, HDL-C, LDL-C 항목에서 두 집단간에 유의한 차이가 있었고, 중성지방은 두 집단간에 유의한 차이가 없었다(P>.05). The present study investigates the effect of a 16-week-long rhythmic exercise on antioxidant activity and blood lipids in elderly women. The subjects were 24 elderly women aged between 60 and 65. They voluntarily participated in experiment at H University Department for Continuing Education. All the subjects were without any obvious signs of ill health in medical examination. None of them took any form of antioxidant medication from 3 months before participating in the experiment, and they were not allowed to take antioxidents throughout the study period. 12 of them who wanted to participate in the exercise program made up an exercise group, while the other 12 who didn't want to perform the exercise made up a control group. After being selected, the subjects of this study had a check-up which consisted of a total antioxidant status test, an MDA(malondialdehyde) level test and blood lipid(Total cholesterol, Triglyceride, LDL-cholesterol, HDL-cholesterol) tests before starting the rhythmic exercise program. They got the same check-up 16 weeks later, right after the final exercise had ended. As for the statistical analysis to confirm the result of this research, SPSSWIN version 10.0 program was applied. In clarifying the difference between the exercise group and the control group, Independent t-test was used. To analyze the experimental effect in each group(before-after difference in each group), Paired t-test was used. The significance level to verify the hypothesis was set at α=.05. Through the experimental process and methods stated above, we obtained the following results: 1. After the rhythmic exercise treatment, there were meaningful differences between the two groups in total antioxidant status(P<.05). There was a significant change in MDA of the exercise group, but no meaningful difference was found between the two groups(P>.05). 2. As for blood lipids, there were meaningful differences between the two groups in Total cholesterol and HDL-cholesterol, LDL-cholesterol but no significant difference was shown in Triglyceride(P>.05).

고강도 운동 시 성별에 따른 혈장 MDA, SOD 및 임파구 DNA 손상 변화

Su-Youn Cho(조수연),Young-Soo Chung(정영수),Yi-Sub Kwak(곽이섭),Hee-Tae Roh(노희태) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

본 연구는 고강도 1회성 운동 시 혈장 MDA와 SOD의 농도변화와 임파구 DNA 손상에 대한 성별의 차이를 평가하는데 목적이 있었다. 본 연구의 목적을 달성하기 위하여 남자 대학생과 여자 대학생을 대상으로 85%VO2max all-out 운동수행에 따른 혈장 MDA와 SOD 그리고 임파구 DNA 손상에 대한 분석을 실시하였으며, 연구 결과에 대한 결론은 다음과 같다. 85%VO2max all-out 운동에 따른 혈장 MDA와 SOD는 운동 종료 시 유의하게 증가하였으며, 통계적으로 유의한 차이는 나타나지 않았으나 남성이 여성에 비해 MDA는 높고 SOD는 낮은 경향을 보였다. 반면 85%VO2max all-out 운동에 따른 임파구 DNA 손상을 알아보기 위해 실시한 comet assay 결과 세 가지 parameter (%DNA in the tail, tail length, tail moment) 모두 운동 종료 시 유의하게 증가하였으며 남성의 %DNA in the tail과 tail length가 여성에 비해 통계적으로 유의하게 높게 나타났다. 따라서 본 연구 결과를 종합해보면 1회성 고강도 운동은 산화적 스트레스를 유발할 수 있으며 남성이 여성에 비해 산화적 손상이 더 크다고 보여진다. 그러나, DNA 손상에는 산화적 스트레스 외에도 체력, 호르몬 수치, 생활습관, 운동 강도 및 지속시간 등 여러 가지 요인들이 영향을 줄 수 있다고 보고되고 있어, 성별에 따른 DNA 손상에 대한 명확한 기전을 제시하기 위해서는 DNA 손상에 영향을 줄 수 있는 여러 요인들과의 관계를 고려한 지속적인 연구들이 필요하다고 생각된다. The purpose of this study was to investigate gender-specific changes of plasma MDA, SOD, and lymphocyte DNA damage during high intensity exercise. In this study, 17 healthy male and 18 healthy female college students ran on a treadmill at 85%VO2max until the point of all-out. Blood-collecting was carried out five times (Rest, Ex-Exha, R0.5h, R4h and R24h), and with the collected blood, plasma malondialdehyde (MDA), superoxide dismutase (SOD), and lymphocyte DNA damage were analyzed. Plasma MDA and SOD concentration increased significantly at the Ex-Exha (p<0.05), and there were no significant differences in gender. For the degree of lymphocyte DNA damage, all %DNA in the tail, tail length and tail moment increased significantly at the Ex-Exha (p<0.05), and %DNA in the tail and tail length were significantly higher in the male group than in the female group (p<0.05). These results suggest that acute high intensity exercise not only causes oxidative stress but also brings about lymphocyte DNA damage. In addition, it was found that males showed higher DNA damage than females in terms of oxidative stress subject to high intensity exercise. Nevertheless, further subsequent studies are required in order to better understand the mechanism behind DNA damage varying with gender, in a way that takes into consideration physical fitness, hormonal level, exercise intensity and duration ? additional factors which might affect DNA damage.

Pilot study of correlation between skin autofluorescence and serum antioxidant enzyme: Skin autofluorescence is negatively associated with levels of malondialdehyde

( June Hyuck Yim ),( Tae In Kim ),( Myong Il Bae ),( Ki Heon Jeong ),( Yunhi Cho ),( Min Kyung Shin ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: The skin autofluorescence(AF) in vivo reflects the accumulation of oxidative stress-derived advanced glycation endproducts(AGEs). Measuring the skin AF is known to serve as quantitiative markers of intrinsic and extrinsic aging. Objectives: This study was aimed to evaluate the change of skin AF in early and late aging. We also examined correlation of the serum antioxdative enzymes and the skin AF. Methods: We measured the skin AF at the photo-exposed and photo-unexposed area of forearm in 37 healthy Korean of various ages. Subjects were divided into two groups, early aging (younger than 40 years olds), and late aging (same or older than 40 years). In addition, we evaluated the correlation with serum catalase, MDA(malondialdehyde), SOD(superoxide dismutase) and vitamin E that are known as antioxidant enzymes. Results: 470 nm peak skin AF significantly increased in the late aging group than early aging group on both photo-exposure and photo-unexposued sites. Also, skin AFon photo-exposure site was showed significantly negatively correlation with MDA level. Conclusion: Measurement of skin AF of photo-exposed area is useful as a noninvasive clinical tool for assessment antioxidant state of individuals, especially reflecting MDA.

Effect of Occupational Exposure to Herbicides on Oxidative Stress in Sprayers

Intayoung, Unchisa,Wunnapuk, Klintean,Kohsuwan, Kanyapak,Sapbamrer, Ratana,Khacha-ananda, Supakit Occupational Safety and Health Research Institute 2021 Safety and health at work Vol.12 No.1

Background: Herbicides such as glyphosate, paraquat, and 2,4-dichlorophenoxyacetic acid have been reported to cause adverse side effects through production of reactive oxygen species. However, there were no data representing the adverse effects of a mixture herbicide usage in farmers, especially the changes in oxidative marker and antioxidant defense. This study aimed to determine the urinary malondialdehyde (MDA) and glutathione (GSH) level in farmers using mixed herbicides. Methods: Ninety-three farmers were recruited, and two spot urine samples (before and after work) were collected. The urinary MDA level was evaluated by thiobarbituric acid reactive substance assay, and the urinary GSH level was determined using the enzymatic recycling method. Results: Sixty-two percent of the participants were men, and 59% of the participants worked in a farm for 20-40 years. The common combinations of herbicide usage were glyphosate with 2,4-dichlorophenoxyacetic acid (36.5%). There was no significant difference between pre- and post-work urinary MDA and GSH levels among the 3 groups of herbicides. However, the urinary MDA levels in farmers using the combination of glyphosate and paraquat were significantly higher than those found in farmers using glyphosate alone. The associated factors with changes in MDA levels found that the exposure intensity index (B = 0.154), the cumulative exposure intensity index (B = 0.023), and wearing gloves while working (B = -2.347) were found to be significantly associated with MDA level. Conclusion: The results suggest that the combined use of glyphosate and paraquat caused a significant increase in urinary MDA levels. Moreover, intensity of exposure to herbicide and wearing gloves were associated with the level of MDA.

운동 전·후 에너지 대사량에 따른 혈중 지질과산화물 차이

김상두(Sang Du Kim),김창균(Chang Gyun Kim) 한국체육교육학회 2002 한국체육교육학회지 Vol.7 No.3

The purpose of this study was to investigate the blood MDA(malondialdehyde) concentration to the volume of energy metabolism in 60%O_2max. The subjects were consisted of college student 12 men, which was expreimented before and after exercise at 300kcal, 400kcal, and 500kcal in 60%O_2max used treadmill by T7 protocol made in Korean Sports Science Institude. The attained data was analyzed the difference it by the independent t-test at before & after exercise and ANOVA by post hoc Turkey HSD with the level of significant 1% & 5%. The results of the study can be summerized in the followings ; First, it was shown the significant defference between before and after exercise at 300kcal, 400kcal, and 500kcal(p<.01), and between 300kcal and 500kcal(p<.05) after exercise in MDA. In conclusion, it was found to increase MDA before and after exercise in exercise intensity of 60%O_2max. But, MDA was slugged at 500kcal after exercise.

운동강도에 따른 MDA와 SOD의 변화

이윤미 ( Yoon Mi Lee ),이소은 ( So Eun Lee ),이재문 ( Jae Mun Lee ),김태영 ( Tae Young Kim ),최승욱 ( Seung Wook Choi ) 한국운동영양학회 2008 Physical Activity and Nutrition (Phys Act Nutr) Vol.12 No.2