Analysis of Partial cDNA Sequence from Human Fetal Liver

Kim, Jae-Wha,Song, Jae-Chan,Lee, In-Ae,Lee, Young-Hee,Nam, Myoung-Soo,Hahn, Yoon-Soo,Chung, Jae-Hoon,Choe, In-Seong Korean Society for Biochemistry and Molecular Biol 1995 Journal of biochemistry and molecular biology Vol.28 No.5

Single-run Partial cDNA sequencing was conducted on 1,592 randomly selected human fetal liver cDNA clones of Korean origin to isolate novel genes related to liver functions. Each partial cDNA sequence determined was analyzed by comparing it with the databases. GenBank, Protein Information Resource (PIR) and SWISS-PROT Protein Sequence Data Bank. From a set of 1.592 cDNA clones reported here, 1,433 (90.0% of the total) were informative cDNA sequences. The other 159 clones were identified as DNA sequences which had originated from the cloning vector. Among 1,433 informative partial cDNA sequences, 851 (59.3%) clones were revealed to be identical to known human genes. These known genes have been classified into 225 different kinds of genes. In addition, 340 clones (23.7%) showed various degrees of homology to previously known human genes. Ninety four (6.6%) clones contained various repeated sequences. Twenty four (1.7%) partial cDNA sequences were found to have considerable homology to known genes from evolutionarily distant organism such as yeast, rice, Arabidopsis, mouse and rat, based on database matches, whereas 124 (8.7%) had no Significant matches. Human homologues to functionally characterized genes from different organisms could be classified as candidates for novel human genes of similar functions. Information from the partial cDNA sequences in this study may facilitate the analysis of genes expressed in human fetal liver.

사람 태아 간의 과립백혈구 형성에 관한 전자현미경적 연구

홍진숙,김경용,이원복,김동창 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.4

연구논문 : 생명과학 ; 사람 제대혈로부터 사람 재조합 SCF 및 IL-4에 의한 CD3+ 세포의 분화 및 증식

이은경 ( Eun Kyung Lee ),민해기 ( Hae Ki Min ),장현욱 ( Hyeun Wook Chang ),나오토모캄베 ( Naotomo Kambe ) 영남대학교 약품개발연구소 2004 영남대학교 약품개발연구소 연구업적집 Vol.14 No.-

Studies on Ganglioside GM3 and Sialidase Activity in Human Fetal Liver

Lee, Young-Sun,Jhon, Gil-Ja 이화여자대학교 이화어문학회 1995 생명과학연구논문집 Vol.6 No.-

Ganglioside GM3 and sialidase activities in human fetal liver have been investigated. Gangliosides were extracted from fetal livers by the Folch-Suzuki method and analyzed by high-performaned thin layer chromatography (HPTLC). GM3 increased, but lactosylceramide (LacCer) decreased predominantly over the developmental stages. Sialidase in human fetal liver was mainly localized in the lysosomal fraction and its activity was high in the earlier stages of development. The optimum pH for this enzyme was 4.3~4.4. Sialidase was more active with the ganglioside mixture than with GM3, sialyllactose or fetuin. Fetal liver changes of the ganglioside GM3 and sialidase activity may be involved in the regulation of cell growth in human fetal liver during development.

Studies on Ganglioside GM3 and Sialidase Activity in Human Fetal Liver

Lee, Young-Sun,Jhon, Gil-Ja Korean Society for Biochemistry and Molecular Biol 1995 Journal of biochemistry and molecular biology Vol.28 No.5

Ganglioside GM3 and sialidase activities in human fetal liver have been investigated. Gangliosides were extracted from fetal livers by the Folch-Suzuki method and analyzed by high-performance thin layer chromatography (HPTLC). GM3 increased, but lactosylceramide (LacCer) decreased predominantly over the developmental stages. Sialidase in human fetal liver was mainly localized in the lysosomal fraction and its activity was high in the earlier stages of development. The optimum pH for this enzyme was 4.3~4.4. Sialidase was more active with the ganglioside mixture than with GM3, sialyllactose or fetuin. Fetal liver sialidase was still active (20% activity) in the presence of 25% methanol. These results suggested that the changes of the ganglioside GM3 and sialidase activity may be involved in the regulation of cell growth in human fetal liver during development.

사람태아 간조직의 형태형성에 관한 연구

등영건,김동희,Deung, Young-Kun,Kim, Dong-Heui 한국현미경학회 1998 Applied microscopy Vol.28 No.3

Hemopoiesis and morphogenesis of the human fetal liver through from 10 to 32 weeks of gestation were investigated by light and electron microscopy. The results obtained were as follows. Hemopoiesis of fetal liver tissue was found from 10 to 32 weeks of gestation, but the hemopoiesis was decreased at 32 weeks of gestation. At the 32 weeks of gestation, matured erythrocytes were observed in the sinusoid, and formation of liver cell cord and portal triad were established. Differentiation of hepatic cell was characterized by the increase of amount of cell organelles within cytoplasm, decrease of hemopoietic cell, morphological change of nuclear envelope from folding form to round form during the developmental period. These results suggest that human fetal liver plays a hematopoietic function until bone marrow and spleen play their function, but morphology of liver at 32 weeks of gestation was differed with structure observed in liver of adult.

태아의 간과 흉선 조직 그리고 조혈모 세포를 이식한 Rag2<SUP>-/-</SUP>γc<SUP>-/-</SUP> Mice에 사람의 면역 세포 형성

강미진(Mijin Kang),주성연(Sung-Yeon Joo),최봉금(Bong-Kum Choi),정다연(Da-Yeon Jung),최호인(Ho-In Choi),박재범(Jae Berm Park),최규성(Gyuseong Choi),권준혁(Choon Hyuck Kwon),김성주(Sung-Joo Kim),조재원(Jae-Won Joh) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.1

Purpose: Many researchers have tried to develop animal models that mimic the human immune system, e.g. a humanized mouse model, to improve the engraftment of hematopoietic stem cells and develop human immune cells in an animal model. This study evaluated the feasibility of the cultured human umbilical cord blood (hUCB)-derived CD34? cells for cell expansion, in Rag2<SUP>-/-</SUP>γc<SUP>-/-</SUP> mice, and establish co-transplantation with human fetal thymus/liver tissue (Thy/Liv) under the kidney capsule. Methods: Co-transplantation of hUCB-derived CD34? cells with Thy/Liv was performed. The hUCB-derived CD34? cells were prepared by freshly thawing (G1) and culturing for 7 days with two types of cytokine combinations (G2, G3). The CD45? cell populations were measured at 6, 8, 10 and 16 weeks in the peripheral blood. The splenocytes were cultured with mitogenic stimuli (PHA -L or IL-2) at 20 weeks posttransplantation, and the proliferation of human immune cells was evaluated. Results: There were no significant differences in the human CD45? cell populations at 6, 8, 10 and 16 weeks post-transplantation between the groups. In the cultured splenocytes at 20 weeks post-transplant with PHA-L or IL-2, there was remarkable expansion of CD3? cells in the three groups. Although no CD19? cells were detected in the spleen, human Ig G was detected in the sera of these mice. Conclusion: The cultured and expanded hUCB-derived cells with cytokine combinations might be a feasible cell source in humanized mouse modeling. In addition, human immune cells can be reconstituted from the co-transplantation of Thy/Liv and cultured hUCB-derived CD34? cells.

사람의 개체발생중 간에서 적혈구모세포의 성숙 - 전자현미경적 연구

김경용,엄학영,양현철,이원복 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.1

Molecular Cloning and Substrate Specificity of Human NeuAc ${\alpha}$2,3Gal${\beta}$ 1,3GalNAc GalNac ${\alpha}$2,6-Sialyltransferase (hST6GalNac IV)

Lee, Young-Choon,Kim, Kyoung-Sook,Kim, Sang-Wan,Min, Kwan-Sik,Kim, Cheorl-Ho,Choo, Young-Kug Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.1

The cDNA encoding human NeuAc ${\alpha}$2,3Gal$\beta$ 1,3GalNAc GalNac ${\alpha}$2,6-Sialyltransferase (hST6GalNac IV) was isolated by screening of human fetal liver cDNA library with a DNA probe generated from the cDNA sequence of mouse ST6Gal NAc IV (mkST6GalNAc IV). The cDNA sequence included an open reading frame coding for 302 amino acids, and comparative analysis of this cDNA with mST6GalNAc IV showed that each sequence of the predicted coding region contains 88% and 85% identifies in nucleotide and amino acid levels, respecively. The primary structure of this enzyme suggested a putative domain structure, like that in other glycosyltransferases, consisting of a short N-terminal cytoplamic domain, a transmembrane domain and a large C-terminal active domain. This enzyme expressed in COS-7 cells echibited transferase activity toward NeuAc ${\alpha}$2,3Gal$\beta$ 1,3GalNAc, fetuin and GM1b, although the activity toward the later is very low, no significant activity being detected toward Gal${\beta}$ 1,3Gal NAc or asialofetuin, the other glycoprotein substrates tested. The $^{14}$ C-sialylated residue of fetuin sialylated by this enzyem with CMP-[$^{14}$C]NeuAc was sensitive to treatment with ${\alpha}$2,8-specific sialidase of Vibrio cholerae but resistant to treatment with ${\alpha}$2,3-specific sialidase (NaNase I), and ${\alpha}$2,3- and ${\alpha}$2,8-specific sialidase of Newcastle disease virus. These results clearly indicated that the expressed enzyme is a type of GalNAc ${\alpha}$2,6-sialyltransferase like mST6GalNAc IV, which requires sialic acid residues linked to Gal${\beta}$1,3GalNAc-residues for its activity.

태아 간 적혈구형성에서 별큰포식세포의 적혈구모세포섬형성 - 투과 및 주사전자현미경적 관찰

이원복,신도식,김경용,Lee, Won-Bok,Shin, Do-Shik,Kim, Kyung-Yong 한국현미경학회 1999 Applied microscopy Vol.29 No.1

The relationship between intravascular erythroblasts and Kupffer cells in the human fetal liver from 11 to 20 week gestation was studied ultrastructurally. The walls of the developing sinusoids consisted of two cell types devoid of basal lamina, the nonfenestrasted endothelial cells and Kupffer cells. Kupffer cells examined were easily identified by their content of phagosmes and their morphological features, and partially proliferated by mitotic division which was different way of proliferation from adult. Some extruded nuclei of acidophilic erythroblasts were trapped within Kupffer cells which exhibited various stages of intracellular digestion of the nuclei. During high activity of human fetal hepatic erythropoiesis, Kupffer cells were found in association with developing erythrob-lasts, which was similar with erythroblastic islands. The developing erythroblasts were partially surrounded by multilaminated membrane system of the Kupffer cell consisting erythroblastic island, or in contact with Kupffer cell via cytoptasmic processes in the sinu-soidal lumen. The presence of these islands was confirmed by transmission and scanning electron microscopic study. The results demonstrate that Kupffer cells in fetal heaptic erythropoiesis phagocytized expelled nuclei and contributed to erythropoiesis mechanically and physiologically by the hypertrophy and the formation of erythroblastic islands. 태생기 간 적혈구형성 중 혈관밖공간의 미성숙 적혈구모세포의 일부는 동굴모세혈관 속공간으로 이주하며, 별큰포식세포에 의해 영향을 받는다. 본 연구에서는 혈관속공간에 이주한 미성숙 적혈구모세포와 별큰포식세포의 관계를 규명하고자 간 적혈구형성의 활성도가 높게 유지되는 태령 제 11주부터 태령 제 20주에 이르는 태아 간과 쥐 태자 간의 연구재료를 대상으로 투과 및 주사전자현미경 관찰을 통해 다음과 같은 결론을 얻었다. 1) 별큰포식세포는 성인 간이나 다른 태령 시기에 비해 비대하였고 많은 세포질돌기들을 갖고 있었다. 이 세포는 부분적으로 세포분열에 의해 증식되고 있었으며 성인 간의 별큰포식세포와 다르게 자가종식을 할 수 있었다. 3) 호산성적혈구모세포에서 탈출된 핵은 별큰포식세포의 속공간쪽과 간세포쪽 세포막에서 포식되었다. 포식된 핵은 주변 부위에 층판이 형성되거나 및 개의 작은 조각으로 파괴되는 등 다양한 소화과정을 보였다. 3) 동굴모세혈관 속공간에서 별큰포식세포는 미성숙적혈구모세포들과 직접으로 접촉하거나, 미성숙 적혈구 모세포들 사이에 별큰포식세포의 긴 세포질돌기가 뻗쳐 있어 골수 적혈구형성에서 관찰되는 적혈구모세포섬과 비슷하였다. 이상의 결과를 종합하면 태아 간 적혈구형성이 왕성한 시기의 별큰포식세포는 적혈구를 포식하는 것 외에도, 세포가 비대해져서 동굴모세혈관을 통한 혈액흐름을 감소시키고, 적혈구모세포섬을 구성하여 태아 간 적혈구형성에서 미성숙 적혈구모세포의 성숙과 관련된 기계적 및 생리학적 기능을 수행한다고 생각된다.