산수유의 영양성분과 항균효과

이순옥(Soon Ok Lee),한삭명(Sag-Myung Han),김혜미(Hye-Mi Kim),정승경(Seung-Kyoung Jeung),최진영(Jin-Young Choi),강일준(Il-Jun Kang) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.7

산수유의 영양성분과 냉면육수 제조시 항균효과를 조사하여 식품소재로써 산수유의 이용성을 증대시키는 기초자료로 삼고자하였다. 산수유의 일반성분은 건량기준으로 탄수화물 87.7%, 조단백질 3.2%, 조지방 4.5%, 회분 4.6%이었다. 산수유의 총아미노산 함량은 2,470 ㎎%로 나타났다. 아미노산 조성으로는 산성아미노산인 aspartic acid가 523㎎%, glutamic acid가 347 ㎎%로 높게 나타났다. 지방산은 총 포화지방산 함량 30.8%, 총 불포화지방산 함량 69.2%로 다가불포화지방산의 함량이 높게 나타났다. 지방산 조성을 살펴보면 linoleic acid가 33.3%로 가장 높았고, 그 다음으로는 palmitic acid (25.1%), linolenic acid(21.6%), oleic acid(13.2%) 순이었다. 무기질 중 칼륨함량이 건물기준으로 2,067.5 ㎎%로 가장 높았고 그 다음으로는 Ca 372.9 ㎎%, Mg 98.4 ㎎% 순이었다. 산수유의 유기산 함량은 gallic acid가 19,478 ㎎%로 가장 그 함량이 높았으며, 그 다음으로는 succinic acid(18,167 ㎎%), malonic acid(14,487 ㎎%), malic acid(13,018 ㎎%) 순이었다. 산수유 3 g을 첨가하여 제조한 냉면육수는 살균효과를 향상시켜줄 뿐 아니라 저장기간에도 균의 생육을 충분히 억제시켰다. 관능검사 결과는산수유 1.5 g을 첨가하여 만든 육수가 색깔, 냄새, 맛 및 전체기호도에서 좋은 영향을 미치는 것으로 나타났다. The chemical components and antimicrobial effects were investigated to provide basic data that will predict the usefulness of Corni fructus as food materials. The carbohydrate, crude protein, lipid and ash contents of Corni fructus were 87.7, 3.2, 4.5 and 4.6% in dry basis, respectively. Total amino acid content of Corni fructus was 2,470 ㎎%. Major amino acids of Corni fructus were aspartic acid (523 ㎎%) and glutamic acid (347 ㎎%). The compositions of total saturated and unsaturated fatty acids of Corni fructus were 30.8% and 69.2%, respectively. Major fatty acids of Corni fructus were linoleic acid (33.3%), palmitic acid (25.1%), linolenic acid (21.6%) and oleic acid (13.2%). The mineral contents of Corni fructus were 2067.5 mg% of K, 372.9 ㎎% of Ca and 98.4 ㎎% of Mg in dry basis. The organic acid contents of Corni fructus were 19,478 ㎎% of formic acid, 18,167 ㎎% of succinic acid, 14,487 ㎎% of malonic acid and 13,018 ㎎% of malic acid. Naengmyon yuksu (beef stock for cold noodles) were prepared with the addition of Corni fructus. Corni fructus added to Naengmyon yuksu inactivated microorganism and inhibited the growth of microorganism during storage at 10℃. Naengmyon yuksu added 1.5 g of Corni fructus showed the highest sensory scores.

Antidiabetic Effects of Corni Fructus Extract in Streptozotocin-Induced Diabetic Rats

Dawei Gao,Qingwang Li,Zhengrong Gao,Lixin Wang 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.4

Purpose: Diabetes is the leading cause of end-stage renal failure. The present study was undertaken to characterize the effects of Corni Fructus on diabetic nephropathy in streptozotocin-induced diabetic rats and their mechanisms. Materials and Methods:Streptozotocin-diabetic rats were orally administrated with Corni Fructus at a dose of 100, 200 or 400 mg/kg body mass for 40 days. Results: Corni Fructus-treated diabetic rats showed significant decreases of blood glucose, urinary protein levels and water consumption. Corni Fructus also reduced serum total cholesterol, total triglyceride and low-density lipoprotein cholesterol levels, and showed a tendency of enhancing high-density lipoprotein cholesterol level. Levels of serum albumin and creatinine in diabetic rats were also significantly reduced by Corni Fructus administration at a dose of 200 and 400 mg/kg body mass compared with non-treated diabetic rats. Corni Fructus increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidose (GSH-px) activities in the kidneys of diabetic rats. Furthermore, Corni Fructus treatment enhanced renal peroxisome proliferator-activated receptor-γ (PPARγ) expression in diabetic rats. Conclusion: These results demonstrated that Corni Fructus may have the potential to protect the animals from diabetic nephropathy by amelioration of oxidative stress and stimulation of PPARγ expression.

Protection Against Hypercholesterolemia by Corni Fructus Extract and Its Related Protective Mechanism

박찬흠,조은주,Takako Yokozawa 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.5

To reduce cardiovascular disease (CVD) by reducing circulating cholesterol concentrations, much attention has been focused on the search for dietary interventions for hypercholesterolemia. Corni Fructus is known to exhibit several biological activities. Therefore, in the present study, its protective effect on diet-induced hypercholesterolemia was studied using a rat model fed 1% cholesterol and 0.5% cholic acid. Corni Fructus extract was administered at an oral dose of 50, 100, or 200mg/kg of body weight/day for 10 days. The administration inhibited the elevation of both systolic and diastolic blood pressure. In addition, it lowered serum total cholesterol levels with a decrease in esterified cholesterol. Moreover, the atherogenic index was decreased in a dose-dependent manner, suggesting its protective role against CVD through regulating cholesterol and lipoprotein levels. The hepatic levels of total and free cholesterol were also reduced by Corni Fructus. This implies that free cholesterol was used for catabolism or efflux. Consequently, the levels of total cholesterol and bile acid in feces were significantly increased. Our present results also showed that the administration of Corni Fructus decreased lipid peroxidation, suggesting a protective effect against oxidative stress induced by hypercholesterolemia. Furthermore, hypercholesterolemic control rats had significantly lower expression of sterol regulatory element-binding protein (SREBP)-2 protein without any difference in SREBP-1 protein expression. On the other hand, the protein expression of SREBP-2 was significantly up-regulated by Corni Fructus. Furthermore, the protein expression of peroxisome proliferator-activated receptor α was elevated, indicating that Corni Fructus would activate fatty acid oxidation. In conclusion, Corni Fructus may protect against CVD by regulating blood pressure, cholesterol levels, and expression of proteins related to lipid metabolism.

추출용매에 따른 복령(Poria cocos Wolf)과 산수유(Corni fructus) 추출물의 생리활성 효과 비교

오희경 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.4

This study was to investigate the antioxidant, anti-inflammatory and neuronal cell protective effects of Poria cocos Wolf and Corni fructus extracted by water and 70% ethanol. Total polyphenol content in water extract of Poria cocos Wolf was significantly higher than those of other extracts. DPPH and ABTS free radical scavenging activity in water extract of Corni fructus was higher than those of other extracts. DPPH and ABTS free radical scavenging activity were increased in a dose-dependent manners. In order to effectively extract total polyphenol contents and anti-oxidant components in Poria cocos Wolf and Corni fructus, hot water extraction method is more efficient than ethanol extraction method. Poria cocos extracts were found to be a superior NO production inhibitory effect compared to Corni fructus extracts. In a neuronal cell viability assay using MPP + , the water extract of Poria cocos Wolf protected against MPP + -induced neurotoxicity than those of Corni fructus extract. It is considered to be a potential functional material with antioxidant, anti-inflammatory and neuronal protective effect against to oxidative stress according to the extract methods of extracting Poria cocos Wolf and Corni fructus. 본 연구는 열수와 에탄올 용매에 따른 복령 및 산수유 추출물의 농도별로 항산화 활성, 항염증 효과 및 신경세포 보호효과에 미치는 영향을 살펴보고자 진행하였다. 추출물들의 총 polyphenol 함량은 복령 열수 추출물에서 가장 높았고, 복령 에탄올 추출물, 산수유 열수 추출물, 산수유 에탄올 추출물 순으로 유의적으로 감소되는 경향을 나타냈다. DPPH 및 ABTS radical 소거 활성능은 추출물의 농도 의존적으로 증가하였고 산수유 열수 추출물은 모든 추출물에서 항산화 활성이 가장 높게 나타났으며, 복령 열수 추출물은 에탄올 추출물에 비해 높은 활성이 확인되었다. 복령과 산수유에 함유된 총 polyphenol과 항산화 성분을 추출하기 위해서는 에탄올 추출방법 보다는 열수 추출방법이 효과적인 방법이라고 사료된다. LPS로 염증이 유도된 RAW 264.7 세포에서 복령추출물이 산수유 추출물에 비해 NO 생성 억제 효과가 우수한 것으로 확인되었다. MPP + 에 의해 유도된 SH-SY5Y 신경세포에 복령 열수 추출물은 산수유 열수 추출물에 비해 산화적 스트레스로부터 신경세포 보호효과가 우수하게 나타났 다. 복령과 산수유 추출방법에 따른 항산화, 항염 및 산화적 스트레스로부터의 신경세포 보호효과를 갖는 기능성 소재로서 가능성이 있다고 사료된다.

산수유를 이용한 저당 산수유잼의 제조 및 품질 특성

박수진(Su-Jin Park),이경은(Gyeong-Eun Lee),김용주(Yong-Joo Kim),정지숙(Ji-Suk Jeong) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.2

본 연구는 산수유 고유의 붉은색과 신맛, 쓴맛을 유지하기 위해 당 함유량을 줄인 산수유잼을 제조하였다. 고형분 농도가 일정한 산수유 퓨레를 제조한 후 이를 이용하여 저당 산수유잼 제조 배합비를 조사하였다. 산수유 퓨레는 과육 무게의 동량 멸균증류수를 첨가하여 제조하였으나 수분 함량은 20~30% 정도만 증가하였다. 펙틴 함량은 0.14%로 매우 낮게 나타나 잼 제조 시 겔화제의 첨가가 필요하였다. 퓨레는 과육에 비해 당도가 30% 수준으로 감소하였으나 pH는 차이가 없었다. 펙틴 첨가로 인한 잼의 pH 변화는 없었다. 퓨레의 L값, a값 및 b값 모두 성숙과 퓨레가 완숙과 퓨레에 비하여 높게 나타났다. 또한 잼 제조 시 성숙과의 함량이 증가할수록 L값, a값 및 b값 모두 증가하였다. 기계적 경도는 펙틴 함량이 증가할수록, 완숙과의 함유량이 증가할수록 산수유잼의 gel strength 및 hardness가 증가하였다. 산수유 성숙과 및 완숙과 퓨레의 DPPH 라디칼 소거능은 100ppm 농도에서 각각 50.96%, 47.92%의 소거 활성을 보였으며, 산수유잼은 1,000 ppm 농도에서 41.24~49.98%의 소거 활성을 보였다. 총 폴리페놀 함량은 92.70~158.52 mg GAE/g으로 완숙과의 첨가량이 증가할수록 폴리페놀 함량은 감소하는 경향으로 산수유 과육이 농후해지면서 폴리페놀 함량은 감소하였다. 전반적인 기호도 평가에서는 펙틴2.0%가 5.43, 성숙과 및 완숙과를 동량 첨가했을 때 5.03으로 가장 높은 점수를 보였다. 이와 같은 결과를 통해 건피에 국한되었던 산수유 가공품이 생과를 활용한다면 다양한 제품 개발이 가능할 것으로 생각된다. Corni fructus is often distributed or processed in the form of dried fruit. However, Corni fructus is hard to develop due to its distinctive sour, bitter, and astringent taste. The aim of this study was to develop a puree to broaden the utilization of fresh Corni fructus. Manufacturing and quality characteristics of Sansuyu jam made from puree were investigated. Seeded Corni fructus pulp consisted of 20 to 26% whole fruit. The moisture and sugar contents of pulp were 52∼63% and 15∼31°Brix, respectively. Sterilized distilled water was added to seeded pulp to achieve a constant solids content in the puree. As the pectin content was low as 0.14±0.01%, gelling agent was added to produce jam. The moisture content of the puree increased to 83∼88%. The sugar content was reduced to 10°Brix. There was no significant difference in pH. DPPH radical scavenging activities of the puree according to ripening rate at a concentration of 100 ppm were 47.92% and 50.96%, respectively. The preference degree was 5.03±0.97 at a ripening ratio of 50:50, 2% pectin, and 0.2% carrageenan. These results imply that Corni fructus pulp puree may be appropriate for development as a natural food product.

한약재복합 추출물의 인간피부섬유아세포 HS68에 대한 항노화 효과

신동철 ( Dong Chul Shin ),김귀철 ( Gwui Cheol Kim ),송시영 ( Si Young Song ),김희진 ( Hee Jin Kim ),양재찬 ( Jae Chan Yang ),이용화 ( Yong Hwa Lee ),김보애 ( Bo Ae Kim ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.2

Objectives : The purpose of this study was to investigate antiaging and antioxidant effects on cultured human skin fibroblast with 80% ethanol extracts of plants including of stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Methods : An ethanol extract of three medicinal plants including stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Extracts were assessed to determine the mechanism of antioxidant and antiaging activities. Antioxidant activity of extract was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. These extracts were tested for cell viability on HS68 skin fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. We investigated the effects of Ultraviolet-B irradiation on cytotoxicity, type 1 collagen, elastin level and oxidative damage in cultured human skin fibroblast (HS68). Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Results : The extracts obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The extracts of complex herbal medicine showed low cytotoxicity as more than 100% cell viability in 100ppm/ml concentration. HS68 fibroblasts were survived 70% at 120 mJ/cm2 UVB irradiation and treated tumor necrosis factor (TNF)-alpha. The levels of aging factors and cytotoxicity were decreased by ethanol extract of complex herbal medicine. Conclusions : These results suggest that ethanol extracts of complex medicinal plants of including of stem of Dendropanax morbifera, Corni fructus and Lycii Fructus may have value as the potential antioxidant and antiaging medicinal plant.

황칠나무, 산수유, 구기자 복합 초임계유체추출물의 항산화 및 항노화 효과

신동철 ( Dong Chul Shin ),김귀철 ( Gwui Cheol Kim ),송시영 ( Si Young Song ),김희진 ( Hee Jin Kim ),양재찬 ( Jae Chan Yang ),김보애 ( Bo Ae Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6

Objectives: The purpose of this study was to investigate antiaging and antioxidant effects on cultured human skin fibroblast with supercritical fluid extracts of Dendropanax morbifera, Corni fructus and Lycii Fructus. Methods : Supercritical fluid extraction (SFE) technique was applied to extract from three medicinal plants including stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Antioxidant activity of extract was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. These extracts were tested for cell viability on HS68 skin fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. We investigated the effects of Ultraviolet-B irradiation on cytotoxicity, type 1 collagen, elastin level and oxidative damage in cultured human skin fibroblast (HS68). Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Results : The extracts obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The supercritical fluid extracts of complex herbal medicine showed low cytotoxicity as more than 100% cell viability in 100ppm/ml concentration. HS68 fibroblasts were survived 70% at 120 mJ/cm2 UVB irradiation and treated tumor necrosis factor (TNF)-alpha. The levels of aging factors and cytotoxicity were decreased by supercritical fluid extract of complex herbal medicine. Conclusions : These results suggest that supercritical fluid extracts may have value as the potential antioxidant and antiaging medicinal plant.

산수유 복용 후 발생한 급성 간질성 신염

전지민 ( Ji Min Jeon ),박용기 ( Yong Ki Park ),전미영 ( Mi Young Jeon ) 대한내과학회 2013 대한내과학회지 Vol.85 No.5

Drug-induced interstitial nephritis is one cause of acute kidney injury. Although traditional remedies have been widely used in South Korea, Corni fructus-induced interstitial nephritis has not been reported in the general population. A 65-year-old female patient was admitted with sudden onset of gross hematuria after ingesting Corni fructus tea for 2 weeks. Her initial lab findings showed a blood urea nitrogen level of 35.1 mg/dL, creatinine level of 1.7 mg/dL, albumin level of 4.5 g/dL, and hemoglobin level of 10.3 g/dL. Urinalysis revealed SG (1.025), blood (many), protein (+), and WBC (0-3/HPF). Her 24-hour total protein level was 1,120 mg/day. No specific abnormalities were found on serum and urine protein electrophoresis. Kidney biopsy demonstrated severe mononuclear cell infiltration into the renal interstitium with mild tubular atrophy and pericapsular fibrosis. Immediate withdrawal of Corni fructus, appropriate education, and conservative management resulted in gradual restoration of her renal function. This case implies that Corni fructus may be a causative allergen that induces acute interstitial nephritis in some patients. (Korean J Med 2013;85:526-529)

산수유 추출물 및 분획물의 항산화 활성과 활성성분 분석

임도연,이경인 한국생약학회 2017 생약학회지 Vol.48 No.3

In this study, analysis of active compounds that are believed to be highly relevant to antioxidant activity was carried out on the methanol extract and its solvent fractions of Corni fructus. The DPPH radical scavenging activity for the comparison of antioxidant activity was higher in order of aqueous fraction > methanol extract > ethyl acetate fraction > n-hexane fraction. It is similar to the order of total polyphenol contents in the samples. As a result of LC-MS analysis, phenolic acid compounds such as caffeic acid, gallic acid and chlorogenic acid and lognin, which is known as a representative active ingredient of Corni fructus, were identified as active compounds. And the antioxidative activity and the total polyphenol content of the extracts and solvent fractions were found to be related to the contents of the compounds. Particularly, it was confirmed that phenolic acid such as caffeic acid contributes to the antioxidative activity of the aqueous fraction of Corni fructus methanol extract.

지역 특산 천연산물 전호, 파고지, 희첨 및 산수유의 동물세포간의 부착 억제효과

신진혁(Jin Hyuk Shin),차구용(Gu Yong Cha),김희진(Hui Jin Kim),황재호(Jae Ho Hwang),한경호(Kyeong Ho Han),서효진(Hyo Jin Seo),김민용(Min Yong Kim),오석중(Suk Jung Oh),김종덕(Jong Deog Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.6

많은 연구를 통해 세포간 부착은 신생혈관형성에 큰 영향을 미치며, 이러한 세포부착억제를 통해 직접적으로 신생혈관형성을 제어할 수 있다. 따라서 본 연구에서는 이러한 신생혈관형성 억제력을 가지는 지역 천연산물을 이용하여 HUVECs 및 U937 cells를 이용한 세포부착 억제정도를 확인하였다. 4가지 천연산물의 세포부착 억제력을 확인하기 위해 세포부착 억제실험을 3가지 방법으로 달리 하여 세포부착 억제정도를 확인한 결과 먼저, IL-1β 및 천연산물이 자극된 HUVECs 에 대한 U937 세포부착 억제실험에서는 전호, 파고지, 희첨 및 산수유의 농도 증가에 따라 전호의 경우 최고 0.2 mg/L에서 276%의 저해율을, 파고지의 경우 5 mg/L에서 220%, 희첨의 경우 10 mg/L에서 158%, 산수유의 경우 20 mg/L에서 132%의 저해효과가 있음을 보였다. 두 번째로 실시한 HUVECs에 대한 PMA 및 천연산물이 자극된 U937 세포부착 억제실험에서는 전호 0.2 mg/L에서 139%, 파고지 5 mg/L에서 442%, 희첨 10 mg/L에서 720%, 산수유 20 mg/L에서 664%의 저해율을 보임을 확인하였다. 마지막으로 실시한 IL-1β 및 천연산물 추출물이 자극된 HUVECs에 대한 천연산물 추출물이 자극된 U937 cells 의 세포부착 억제실험의 경우, 전호 0.2 mg/L에서 286%, 파고지 5 mg/L에서 146%, 희첨 10 mg/L에서 436%, 산수유 20 mg/L에서 297%의 저해율을 보였다. 이상의 3가지 방법으로 실시한 세포부착 억제실험에서 모두 각각의 천연산물 추출물의 농도증가에 따라 현저하게 그 부착력이 억제됨을 확인하였다. 따라서, 본 연구에서 사용된 전호, 파고지, 희첨 및 산수유는 세포부착을 억제함으로서, 신생혈관형성과 관련된 암 및 비만의 근본적인 치료제제로써 사용 가능함을 시사한다. For investigation of anti-angiogenesis mechanism of Anthrisci radix, Psoraleae semen, Siegesbeckiae herba and Corni fructus, anti-cell adhesion experiment was performed. The adhesion of U937 cells to IL-1β-stimulated HUVECs was completely suppressed by 276% at 0.2 mg/L of Anthrisci radix, 220% at 5 mg/L of Psoraleae semen, 158% at 10 mg/L of Siegesbeckiae herba and 132% at 20 mg/L of Corni fructus, respectively. And the adhesion of PMA-chemical stimulated U937 cells to HUVECs, it was inhibited 139% at 0.2 mg/L of Anthrisci radix, 442% at 5 mg/L of Psoraleae semen, 720% at 10 mg/L of Siegesbeckiae herba and 664% at 20 mg/L of Corni fructus. Also, the adhesion of chemical stimulated U937 cells to IL-1β/chemical stimullated HUVECs, it was inhibited by 286% at 0.2 mg/L of Anthrisci radix, 146% at 5 mg/L of Psoraleae semen, 436% at 10 mg/L of Siegesbeckiae herba and 297% at 20 mg/L of Corni fructus, respectively. It would be a useful substance for anti-cell adhesion based on anti-angiogenesis for anti-obesity and anti-cancer.