치아우식증의 조기진단을 위한 고감도 우식활성검사 모형개발

이상호,이창섭 大韓小兒齒科學會 2000 大韓小兒齒科學會誌 Vol.27 No.1

초기 우식병소와 건전한 치질은 광학적 특성이 다르다는 사실에 근거하여 치과 치료용으로 개발된 가시광선 영역의 아르곤 레이저의 형광현상, 즉 물체에 조사되어 반사된 빛은 물체의 광학적 특성에 따라 파장이 달라지는 특성을 이용하여, 우식 치질과 건전한 치질간의 광학적 특성의 차이를 아르곤 레이저 빛을 통한 색조의 차이로 유도. 전환하는 시스템을 개발하고 이를 이용하여 초기 치아우식 병소를 조기에 시각적으로 탐지하여 data화 하는 새로운 우식활성검사법을 개발하는데 본 연구의 목적이 있다. 연구는 3단계로 나누어 1단계는 아르곤 레이저 형광법의 초기 우식병소 탐지능력을 평가하였으며 2단계는 임상에서 아동들을 대상으로 아르곤 레이저 형광법을 이용하여 초기 우식병소가 있는 치아의 갯수를 집계하고 이 수치에 대한 우식활성검사법인 Cariescreen?? test, 그리고 일종의 구강환경검사인 우식경험치아의 수(dDfFtT)검사와의 상관성을 평가하였다. 3단계는 이와 같은 자료를 기준으로 하여 레이저 형광법을 이용하여 검사할 수 있는 새로운 우식활성검사 모델을 만들고 이와 같은 검사법의 민감도, 타당도, 진단력 등을 분석하여 진단학적 측면에서 활용 가능성을 평가하고자 하였으며 그 결과는 다음과 같다. 초기 우식병소의 조직학적 깊이가 증가될수록 레이저 형광법을 이용하여 측정한 광밀도가 증가하였으며 이들 사이에는 높은 상관관계(=0.7015)가 있었다. 또한 통계학적으로 회귀분석을 시행한 결과 병소의 깊이와 광밀도와 사이에는 정비례 관계의 직선방정식 관계를 나타내었다. 레이저 형광법을 이용한 우식활성검사법은 Cariescreen?? test그리고 dDfFtT검사 등과 높은 상관관계를 나타냈다. 또한 레이저 형광법을 이용한 우식활성 검사법은 dDfFtT검사를 기준으로 48%의 민감도, 52%의 특이도, 45%의 진단력을 보였으며 Cariescreen?? test를 기준으로 48%의 민감도, 51%의 특이도, 36%의 진단력을 보였다. 이상의 결과를 종합하면 레이저 형광법을 이용하여 측정한 초기 우시병소의 광밀도는 실제 초기 우식병소의 조직학적 병소의 깊이를 평가하는데 높은 진단학적 신뢰성을 보여주고 있으며 레이저 형광법을 이용한 우식활성 검사는 진단학적 지표를 기준으로 볼 때, 그리고 검사절차와 시간, 비용 등의 면으로 볼 때 향후 임상적으로 활용 가능성이 높은 것으로 사료된다. The purpose of this study is to develop the system which convert the optical difference of teeth texture between intact enamel and incipient caries lesion into shade difference by laser fouorescence and to develop new and simple caries activity test using laser fluorescence. The experimental design of this study consists of three parts. In first part, a new method for the in vitro assessment of changes in initial enamel caries lesion of Bovine teeth using laser fluorescence is tested. In second part, in vivo assessment undertaken. Number of teeth which showed incipient carious lesion on buccal surface examined by laser fluorescence was compared with the caries activity test of Cariescreen test and other oral environmental test of dDfFtT. In third part, new caries activity test measured by laser fluorescence was developed on the basis of above results and evaluated the sensitivity, specificity, and diagnostic power. Optical density measured by laser fluorescence was increased as increasing the depth of incipient carious lesion and showed high correlation (γ=0.7015)with lesion depth. Optical density showed direct proportion to lesion depth. Linear equation was obtained between the optical density and the lesion depth by regression analysis. The result of caries activity test with laser fluorescence showed high correlation with those of Cariescreen test and dDfFtT examination. Caries activity test with laser fluorescence showed 48% of sensitivity, 52% of specificity, and 45% of diagnostic power on the basis of dDfFtT examination, and also showed 48% of sensitivity, 51% of specificity, and 36% of diagnostic power on the basis of Cariescreen test. In regard above result, caries activity test with laser fluorescence considered to be reliable for caries activity test compared with other oral environmental test. and it was also considered to be practical because it would be simple, inexpensive, and time saving method.

Sulfisomidine배합 산성고자당액상배지검사의 우식예측타당도에 관한 종단 연구: 3년 후 결과

나자영 ( Ja-young Na ),박선화 ( Sun-wha Park ),주현정 ( Hyun-jeong Ju ),김인자 ( In-ja Kim ),이흥수 ( Heung-soo Lee ) 대한구강보건학회 2016 大韓口腔保健學會誌 Vol.40 No.3

Objectives: The purpose of this study was to evaluate the prediction validity of the caries activity test with a sulfisomidine mixture (SAHS test). Methods: This longitudinal follow-up study was conducted for 3 years. The subjects were 155 elementary schoolchildren. Oral examination was performed by examining each tooth surface of the subjects. The number of teeth with new caries lesions was calculated by comparing between the baseline data of the initial oral examination and the results of the second oral examination performed after 3 years. The Dentocult SM test was used as the reference in the analysis of the caries prediction validity of the SAHS test. The items of the validity test for carries prediction were as follows: sensitivity, specificity, predictive value, and likelihood ratio Results: The correlation between new caries lesions and the SAHS test scores was greater than that between new caries lesions and the Dentocult SM test scores. The receiver-operating analysis revealed that the area under the curve of the SAHS test was higher than that of the Dentocult SM test. The caries prediction validity of the SAHS test (grade 12) was as follows: sensitivity, 0.71-0.70; specificity, 0.60-0.58; positive predictive value, 0.79-0.78; negative predictive value, 0.49 (screening criterion 5). The SAHS test scores were similar to or higher than the scores in the items of the Dentocult SM test. Conclusions: The SAHS test is considered useful for clinical applications.

20대 여대생을 대상으로 한 치아우식활성검사 연구

윤미숙,윤혜정 한국치위생과학회 2010 치위생과학회지 Vol.10 No.6

This study surveyed salivary flow rate, salivary viscosity, and salivary buffering capacity in order to intensively analyze salivary factors among factors of occurrence in dental caries for finding mutually different factors that function in occurrence of dental caries depending on each individual. Even the acid body within dental plaque has great influence upon dental caries. Thus, the comparative analysis was carried out by surveying the hydrogen ion concentration in dental plaque. The following results were obtained in this study. 1. The average decayed teeth in the survey subjects stood at 1.67 piece. The extracted teeth caused by dental caries stood at 0.47 piece. The filled teeth were indicated to be 6.31 pieces. Accordingly, the average permanent dental caries experience teeth were surveyed to be 8.44 pieces. 2. The results according to dental caries activity test method were indicated to be 12.56±4.15ml for the average stimulated salivary flow rate, 3.89±1.83ml for non-stimulated salivary flow rate, 1.49±0.69 for salivary viscosity, and 8.51±2.44 for salivary buffering capacity. The hydrogen ion concentration test in dental plaque was indicated to be 5.62±0.50 for before brushing teeth, 5.23±0.58 for 5 minutes after brushing teeth, 5.25±0.56 for 10 minutes after brushing teeth, 5.29±0.62 for 15 minutes after brushing teeth, 5.34±0.58 for 20 minutes after brushing teeth, 5.40±0.53 for 25 minutes after brushing teeth, and 5.61±0.59 for 30 minutes after brushing teeth. 3. Stimulated salivary and non-stimulated salivary flow rate,salivary viscosity, and salivary buffering capacity were indicated to be higher in group with non-caries than group with caries. However, it was statistically insignificant. The hydrogen ion concentration in dental plaque showed wholly statistical significant in the relationship with people with dental caries under progression. However, people without dental caries were indicated to be higher than people with dental caries. 4. As for correlation between caries activity test methods, the stimulated salivary flow rate had significantly positive correlation with non-stimulated salivary flow rate(p<0.001). Non-stimulated salivary flow rate showed negative correlation with salivary buffering capacity(p<0.01). The hydrogen ion concentration test in dental plaque showed positive correlation according to the passage of time after brushing teeth. However, there was no significant correlation with salivary viscosity and salivary buffering capacity(p>0.05).

광학적 치아우식활성 검사법의 신뢰도에 관한 연구

박철홍,이난영,이상호 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.4

The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using LED fluorescence. The subjects of this study were 55 children of 6{sim}7 years old. LED light were irradiated to labial or buccal surface of all teeth. Fluorescence from initial carious lesion of teeth illuminated by an LED light was observed through barrier filter and the number of teeth showing lesion, size and position of lesion were counted. Streptococcus mutans colony counting and dDfFtT rate test were also done and their correlation was compared. And then specificity, sensitivity, diagnostic power of optical caries activity test using LED light were evaluated. 1. There was positive correlation({gamma}=0.43) between LED fluorescence test and Streptococcus mutans count(P<0.05). 2. When visual examination was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 76.1%, and 100%. 3. When dDfFtT rate was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 88.9%, 47.8%, and 95.7%. 4. When S. mutans colony counting was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 58.7%, and 100%. Considering the above results, optical caries activity test using LED light could be regarded as a practical method because of its close relationship with microbiological caries activity test. 본 연구는 LED 형광법을 이용하여 각 개인의 우식활성도를 측정할 수 있는지를 규명하기 위해 6{sim}7세의 아동 55명을 대상으로 치아의 순면과 협면에 LED 광을 조사하고 특수 필터를 사용하여 초기 치아우식증이 관찰되는 치아의 수와 우식의 크기, 위치 등을 기록하고, 이와 같은 초기 치아우식증의 수를 측정하는 우식활성검사법과 기존의 우식활성도 측정방법인 dDfFtT rate, Streptococcus mutans colony count와 상관성을 비교, 평가하고, LED 형광법을 이용한 우식활성검사의 특이도, 민감도, 진단력을 평가하였다. 1. LED 형광법을 이용한 우식활성도 측정법은 기존의 Streptococcus mutans colony count 검사법과 비교적 높은 상관관계를 보였다({gamma}=0.43,;P<0.05). 2. 육안검사를 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 100%, 76.1%, 100%이었다. 3. dDfFtT를 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 88.9%, 47.8%, 95.7%이었다. 4. S. mutans 측정법을 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 100%, 58.7%, 100%이었다. 이상의 결과를 종합하여 볼 때 LED광중합기를 이용한 광학적 치아우식활성 검사법은 미생물학적 치아우식활성 검사법과 비교하여 유의한 상관관계를 보여 임상에서 활용 가능성이 클 것으로 사료된다.

Cariostat를 이용한 아동의 우식활동에 관한 임상적 연구

백병주,양정숙,이영수,양연미,김재곤,Baik, Byeong-Ju,Yang, Jeong-Suk,Lee, Young-Su,Yang, Yeon-Mi,Kim, Jae-Gon 대한소아치과학회 1998 大韓小兒齒科學會誌 Vol.25 No.3

The purpose of the study was to determine the ability of the caries activity test, 'Cariostat' to identify preschool children with dental caries. The subjects of this study were 76 children of 3 to 5 years of age. Oral examination was carried out to assess the caries experience using a mirror and explorer. In all subjects buccal plaque samples were obtained, incubated, and scored as the manufacturer's instructions for the Cariostat test. Statistical analyses were used with the Crosstabulation. The test group exhibited mean dft index 5.21, while 27.63% of subjects were caries free and had no restorations. The sensitivity of the Cariostat test was found to be 98.8% while the specificity was found to be 19.05% The results showed that caries activity measured by visual reading was highly significant to the Cariostat score and dft index(P<0.001). The Cariostat method has indicated the future possible occurrence of caries. The use of this method is meant to require in the caries prevention program.

Realtime PCR법과 세균배양법을 이용한 치아우식세균 검사법 비교 연구

윤정희 ( Joung-hee Yun ),박지현 ( Ji-hyeon Park ),조자원 ( Ja-won Cho ),김성원 ( Sung-won Kim ) 대한예방치과·구강보건학회 2020 大韓口腔保健學會誌 Vol.44 No.3

Objectives: This study aimed to compare the results of conventional methodology and real-time polymerase chain reaction (PCR) for acid-producing bacteria and identify the appropriate method for caries activity testing. Methods: Oral examination and caries activity tests, such as the CRT® Bacteria test and modified Snyder test, were conducted on 33 middle school students in Cheongju, South Korea. Pearson correlation analysis was subsequently performed with a significance level of 5%. Results: The amount of Streptococcus mutans determined using the CRT® Bacteria test was highly correlated with the decayed teeth (DT) index (R=0.570, P<0.05); decayed, missing, and filled teeth (DMFT) index (R=0.376, P<0.05); and dental health capacity of the first permanent molar (R=-0.395, P<0.05). The amount of Streptococcus mutans determined using real-time PCR was significantly associated with the DT index (R=0.528, P<0.05), DMFT index (R=0.369, P<0.05), and dental health capacity of the first permanent molar (R=-0.426, P =0.013). Conclusions: There was a significant correlation between the amount and the type of bacteria between the CRT® Bacteria test and real-time PCR; therefore, these tests were found to be more appropriate to determine caries activity than the modified Snyder test. This study suggests that realtime PCR is a better technique for detecting caries activities than conventional techniques, such as the CRT® Bacteria test and modified Snyder test, because it is easy to use and provides accurate results.

K대학 여대생을 대상으로 한 치아우식활성검사 비교조사 연구

최은미,송윤신,장희경,최정이 경복대학 2002 京福論叢 Vol.6 No.-

본 연구는 치아우식증 발생요인 중 타액요인을 집중적으로 분석하고자 타액분비율, 타액점조도, 타액완충능, 스나이더, 구강내 포도당잔류검사를 조사하였으며, 특히 20내 남성의 치아우식증 발생요인에 대한 연구자료와 타액분비율과 타액내 pH 변화에 대한 조사 및 스나이더검사 간의 임상적 비교 조사 등의 연구를 통하여 자료를 비교 분석할 수 있었다. 또한 구강내 포도당잔류검사를 조사한 결과 등 수종의 실험연구의 경험적 연구 결과를 가지고, 한국의 여대생들과 상호관계를 비교 분석하고자 하였다. 본 연구의 조사대상은 여대생 160명을 대상으로 하였으며, 수집된 자료는 빈도, 백분율 분석으로 유의성을 검증하였다. 본 연구에서 조사된 결과는 다음과 같다. 1. 전체 조사대상자의 우식치아 수는 평균 3.23개로 조사되었고, 발거치아는 0.22개로 조사되었으며, 충전치아는 5.06개로 나타났다. 2. 전체 조사대상자의 평균 영구치 우식경험치아는 8.52개로 조사되었고, 평균 완충능력은 10.54로 나타났다. 3. 구강내 포도당잔류시간을 측정한 결과 평균 12.36 분으로 조사되었다. 4. 스나이더 검사방법 결과 조사대상자의 평균 치아우식활성 정도는 35.43 시간으로 판정기준에 의하여 중등도 활성으로 나타났다. 5. 자극성 타액분비량 및 타액점조도와 진행중인 치아우식증 보유집단과 집단간의 비교에서는 차이가 있음을 볼 수 있었다(P<0.01). 6. 비자극성 타액분비량, 자극성 타액분비량 및 구강내 포도당잔류시간 측정검사와 치아우식활성집단간에는 유의한 수준으로 나타났다(P<0.01). 7. 타액점조도에서도 치아우식활성집단간에는 유의한 수준으로 나타났다(P<0.05). 이와 같은 절과를 볼 때, 향후 연구논제에 세계보건기구(WHO)에서 기초조사로 조사되는 연령군인 12세 아동의 치아우식증과 치아우식활성과의 관계를 규명하고자 하는 노력이 있다면 치아우식증 발생요인을 구체적으로 제시할 수 있으리라 사료된다. This study was performed to investigate saliva flow rate, saliva buffer capacity, snyder test, glucose residuum test in the oral cavity in order to analyze 'saliva factor' intensively among the causing factor of dental caries. Especially through study data on causing factor of dental caries in male twenties, examination on pH changing rate in the saliva and saliva flow rate, clinical comparative study between Snyder tests, we have made a comparative analysis. Also, based on many kinds of exparimental study results from examination on glucose residuum test in the oral cavity, etc, we have intention to analyze comparatively interactive relationship of korean female college students. The subject of this study is 160 female college students, and we verified the confidence of our study data by the frequency, centesimal analysis. The results were as follows ; 1. Among the total subjects, the average of caries teeth were 3.23, extracted teeth 0.22, filling teeth 5.06. 2. Among the total subjects, the average of permanent teeth with experience of dental caries were 8.52, average buffering capacity 10.54. 3. The average minutes of glucose residual time in the oral cavity were examined 12.36 minutes. 4. On the result of Snyder examination method, the average activative degrees of dental caries were 35.43 hours rated medium level by the standard of decision. 5. There are some differencies between stimulative saliva flow, saliva adherence degree and the group of dental caries in progress. 6. There are significant results between non-stimulative saliva secretion, stimulative saliva flow, glucose remaining time test in the oral cavity and the group of dental caries in activation. 7. There are significant results between groups of dental caries in activation about saliva adherence degree. According to the results, if there is any effort in the future study theme to investigate the relationship between 12 year-old children's dental caries whose age WHO has made a basic surveying subject and activation of dental canes, we can present the causing factor of dental caries concretely.

Cariostat를 이용한 아동의 우식활성에 관한 임상적 연구

김재곤,이영수,양정숙,양연미,백병주 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

The purpose of the study was to determine the ability of the caries activity test, 'Car-iostat'to identify preschool children with dental caries, The subjects of this study were 76 children of 3 to 5 years of age. Oral examination was carried out to out to assess the caries experience using a mirror and explorer. In all subjects buccal plaque samples were obtained, incubated. and scored as the manufacturer's instructions for the cariostat test. statistical analyses were used with the Crosstabulation. The test group exhibited mean dft index 5.21, while 27.63% of subjects were caries free and had no restorations. The sensitivity of the Cariostat test was found to be 98.8% while the specifioity was found to be 19.05% The results showed that caries activity measured by visual reading was highly significant to the Cariostat score and dft index(P<0.001). the Cariostat method has indicated the future possible occurrence of caries. The use of this method is meant to require in the caries prevention program.

Caries Activity and Salivaty pH after intalking several kinds of beverages

Hwang, Sun-young 대한임상예방치과학회 2008 International Journal of Clinical Preventive Denti Vol.4 No.1

Objectives : The authors have performed the clinical test for beverages influenced to caries activity. Methods : 30 volunteers age in 20S to 40S were participated for the study for 4 days by Modified Snyder Test and salivary pH test before and 3 or 30 minutes after the beverage, YoKurt and 33% Xylitol solution were used as the selected beverages, and the results of Modified Snyder Test and salivary pH were measured to compare each others. Results: The average score of Modified Snyder Test on 48 hours incubation was lover in Xylitol solution intake group than those of the other groups, both in 3 and 30 minute's after drinking each beverage. The average score of the 3day's sum of the results for Modified Snyder test was lower in Xylitol solution intake group than those of the other group (p<0.05), both in 3 and 30 minute's after drinking each beverage. pH level was higher in Xylitol solution intake group than those of other groups, both in 3 minutes and 30 minutes after drinking each beverage. pH level of Yogurt intake group was revealed as higher those of the results of the group in 3 minutes after Cola and Alkaline beverage intke (p<0.05) and in 30 minutes after Alcaline beverage intake. Conclusion: It was recommended to use Xylitol contained beverage and to limit to use the beverages, in order to prevent the dental caries.

Quantitative Light-Induced Fluorescence-Digital과 Cariview의 우식예측 연구에 대한 활용도 평가

이수영 ( Su Young Lee ),임순연 ( Soon Ryun Lim ),배현숙 ( Hyun Sook Bae ) 한국치위생과학회 2013 치위생과학회지 Vol.13 No.4

The purpose of this study was to evaluate the correlation between caries experience, Quantitative Light-Induced Fluorescence-Digital (QLF-D) redings and new caries activity test (Cariview) results in preschool children. Fifty-seven healthy kindergarten children (male 28, female 29) were participated this study. The calibrated dentist investigated the caries experience of children and new caries activity test. Cariview samples were incubated in the activated medium at 37oC for 48 hours. All QLF-D taking and readings were performed by one experienced and trained operator under identical conditions in a dental unit chair located in a darkened room. Analysis range was limited to the maxillary and mandibular anterior teeth. QLF-D redings (white spot and dental plaque) were analysed using QLF system. The dft index had a relatively high correlation with the QLF-D redings (white spot: r=0.617, simple plaque score: r=0.500) (p<0.01). Also, there was significant correlation between dft index and the Cariview score (r=0.286) (p<0.05). However, the Cariview score had no significant correlation between dt index and ft index (p>0.05). QLF-D can be evaluated objectively the initial caries lesions and dental plaque correlated with caries experience. Therefore, QLF-D will be useful to the study of caries prediction.