알쯔하이머병과 혈관성치매의 뇌척수액 Aspartate Aminotransferase

김돈수,곽용태 대한노인병학회 2001 Annals of geriatric medicine and research Vol.5 No.1

C형 간염에 의한 간경변 환자에서 간암 표지자로서 혈청 알파태아단백의 유용성: 간암 발생 이외에 알파태아단백 상승에 영향을 미치는 인자 분석

김경아 ( Kyung Ah Kim ),이준성 ( June Sung Lee ),정은숙 ( Eun Sook Jung ),김종연 ( Jong Yeon Kim ),배원기 ( Won Ki Bae ),김남훈 ( Nam Hoon Kim ),문영수 ( Young Soo Moon ) 대한소화기학회 2006 대한소화기학회지 Vol.48 No.5

목적: 혈청 알파태아단백은 만성 간질환자에서 간암 진단을 위한 표지자로 널리 이용되고 있다. 그러나 검사의 민감도가 낮고, 간세포 손상이 심할 경우 특이도가 낮아지며 만성 간질환의 원인에 따라 진단 의의가 달라질 수 있다. 우리나라에서는 B형간염과 연관된 간암이 가장 많아 혈청 알파태아단백에 대한 연구는 대부분 B형간염과 연관된 간질환을 대상으로 하였다. 이번 연구에서는 만성 C형간염에 의한 간경변 환자들을 대상으로 간암 발생 이외에 혈청 알파태아단백 상승에 영향을 미칠 수 있는 인자들을 분석하여 이들 인자들에 따른 간암 진단을 위한 혈청 알파태아단백의 예민도와 특이도를 알아보고자 하였다. 대상 및 방법: C형 간염에 의한 간암 환자와 간경변 환자의 의무기록을 후향으로 분석하였다. 간경변 환자는 혈청 알파태아단백이 측정된 후 6개월 이상 추적되어 간암 발생을 배제할 수 있는 예들로 한정하였다. 결과: 55명의 간암 환자와 62명의 간경변 환자를 분석하였다. 알파태아단백 20 ng/㎖를 기준으로 간암 진단에 대한 민감도 72.7%, 특이도 59.7%였다. 알파태아 단백 100 ng/㎖를 기준으로 할 때 민감도 47.3%, 특이도 92.5%였다. 간경변군에서 혈청 AST 값이 혈청 알파태아단백과 연관성을 보여 AST 값에 따라 분석하였다. AST가 정상의 2배 이하인 군에서는 알파태아단백 100 ng/㎖ 이상에서 특이도 100%였고 AST가 정상의 2배 초과한 군에서는 알파태아단백 100, 200 ng/㎖ 이상에서 특이도가 각각 85%, 95%였다. 결론: 만성 C형간염에 의한 간경변 환자에서 AST 값이 혈청 알파태아단백과 연관성이 있었다. AST가 정상의 2배 이하인 경우에는 혈청 알파태아단백 100 ng/㎖ 이상일 때 간암을 확진할 수 있었으나, AST가 2배 초과한 경우에는 혈청 알파태아단백의 특이도는 감소하였다. Background/Aims: Serum α-fetoprotein (AFP) is frequently used for the diagnosis of hepatocellular carcinoma (HCC). Most available data concerning AFP came from studies of patients with chronic hepatitis B or mixed etiologies. Studies concerning the diagnostic value of AFP for HCV-related liver cirrhosis (LC) are limited. We evaluated the factors influencing AFP elevation in the absence of HCC and analyzed the diagnostic value of serum AFP in HCC surveillance of HCV-related LC patients. Methods: We enrolled 55 patients of HCV-related LC with HCC and 62 patients without HCC as a case-control study were analyzed. The sensitivity and specificity were calculated and the clinical and biochemical factors influencing serum AFP levels. Results: The sensitivities and specificities of serum AFP for the detection of HCC in HCV-related LC were 72.7% and 59.7% for AFP≥20 ng/㎖, and 47.3% and 92.5% for AFP≥100ng/㎖, respectively. Elevated serum AST was independently associated with elevated serum AFP level in HCV-related LC. In cases of AST≤2×upper limit of normal (ULN), the specificity of AFP≥100 ng/㎖ for the diagnosis of HCC was 100%. However, in case of AST>2×ULN, the specificity was 85.0% for AFP≥100 ng/㎖ and 95.0% for AFP≥200 ng/㎖. Conclusions: Serum AST levels influence serum AFP level in HCV-related LC. In cases of AST≤2×ULN, AFP greater than 100 ng/㎖ highly indicates HCC in HCV-related LC, but not in case of AST>2×ULN. (Korean J Gastroenterol2006;48:321-326)

부자 및 천오를 포함한 한약이 간 손상 수치에 미치는 영향

장자원,김지윤,민성순,송수진,김기탁,황원덕,Jang, Ja-Won,Kim, Ji-Yun,Min, Sung-Soon,Song, Su-Jin,Kim, Ki-Tak,Hwang, Won-Duk 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.4

Objectives : Boo-ja and Cheon-o are not commonly prescribed, but are necessary for some clinical conditions, dispite the fact that some negative effects have been known to occur with these medicines. Hence, consequences for aspartate aminotransferase(AST), alanine aminotransferase(ALT) due to herbal medicines, including boo-ja and cheon-o are here reported. Methods : From January 2005 to July 2005, results were analyzed for 36 inpatients belonging to the sixth internal medicine department of Dongeui Oriental Medical Hospital who took herbal medicine, including boo-ja and cheon-o, over 20 days. The study is about the comparison and the investigation of LFT, and all results were taken upon their hospitalization and upon their departure. The standard of liver injury was used as a standard for their examinations. Results were as follows : No Change in normal limit : 28 of the 36 Increased AST. ALT from when they were hospitalized : 2 of 36 Normalized AST. ALT from when they were hospitalized with results higher than normal : 6 of 36 These results show no side-effects, therefore suggest that these herbal medicines, including boo-ja and cheon-o, do not cause adverse side-effects for patients of this kind if administered as was done in this study.

Effect of Aerobic Exercise and Gym-ball Exercise on the Liver Function Test Index, Adipokines, and Cardiovascular Risk Factors in Obese Children with Nonalcoholic Fatty Liver Disease

Sung Soo Lee(이성수) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

본 연구에서는 12주간 비알코올성 지방간을 가진 비만아동을 대상으로 유산소 운동군과 짐볼 운동군으로 나누어 운동을 실시한 결과 체중, BMI, 체지방율이 유의하게 감소하였다. 또한 Adiponectin의 증가와 Leptin의 감소로 인해 인슐린저항성과 혈당, 인슐린 농도도 유의하게 감소하는 것으로 나타났다. 또한 이러한 Adipokine들의 긍정적인 기능들로 인하여 비알콜성 지방간을 평가하는 간 기능 평가지수인 AST와 ALT도 감소하는 것으로 나타났다. 결론적으로 비알코올성 지방간을 가진 비만아동뿐만 아니라 비알코올성 지방간 환자에 있어 유산소운동 뿐만 아니라 짐볼 운동에서도 운동효과가 유사하게 나타났으므로 규칙적인 운동은 심폐체력 증진 및 혈중지질의 감소와 함께 혈중 Adiponectin 발현을 증가시킴으로서 인슐린저항성 개선을 통하여 비알코올성 지방간 기능 개선 뿐만 아니라 비만으로 인한 당뇨병, 심혈관 질환 발병의 예방에도 도움이 될 수 있을 것으로 기대된다. The aim of the present study was to investigate effects of a 12-week aerobic exercise training program and a gym-ball exercise training program on body composition, aspartate aminotransferase (AST), alanine aminotransferase (ALT), adipokines, and cardiovascular risk factors in obese children with nonalcoholic fatty liver disease. The subjects were separated into two groups, an aerobic exercise group (n=10), which practiced moderate aerobic exercise training for 12 weeks, and a gym-ball exercise group (n=13), which practiced resistance exercise training for 12 weeks. The results of the analyses are as follows: Weight, body mass index, and body fat were significantly lower (p <0.01, respectively), whereas the VO2 max was higher in both groups (p <0.01). Fasting glucose, insulin and HOMA-IR levels were significantly decreased in the gym-ball exercise group (p <0.05), whereas adiponectin, AST, and ALT levels were significantly increased (p <0.05, p <0.001, p <0.001, respectively) in both groups after the 12-week exercise training program. In addition, our results showed that HOMA-IR, insulin, and concentrations of C-reactive protein (CRP) were significantly lower in both groups. They demonstrate that a 12-week program of regular aerobic exercise or gym-ball exercise yields beneficial effects such as an amelioration of cardiovascular risk factors, body indices, and liver function in obese children with nonalcoholic fatty liver disease.

발효울금의 갈락토사민 투여에 의한 흰쥐의 간독성에 대한 보효 효과

김용재(Yongjae Kim),유양희(Yanghee You),전우진(Woojin Jun) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.6

본 연구는 울금의 산업적 실용화를 극대화시키기 위해 Aspergillus oryzae를 이용하여 산업용 규격에서 생산된 발효울금의 일반성분 및 미량성분 분석과 발효울금을 이용하여 동물모델에서 14일간 예비 투여하고 GalN으로 급성 간독성을 유발한 후 혈액 중 간기능 지표물질의 변화 및 간조직의 조직학적 관찰을 통해 GalN로 유발된 급성 간독성에 발효울금의 간기능 보호효과를 평가하고자 하였다. 발효울금의 일반성분은 수분 0.15%, 조지방 4.68%, 조단백 4.35%, 조섬유 6.92%, 조회분은 6.83%로 분석되었다. 발효울금의 미량성분은 P, Mg, Ca 순으로 분석되었다. 간기능 지표효소인 AST와 ALT의 활성은 발효울금 300 ㎎/㎏/day과 silymarin 150 ㎎/㎏/day를 투여한 군에서 GalN만을 투여한 군과 비교하여 통계적으로 유의한 결과를 나타내어 발효울금 300 ㎎/㎏/day의 예비 투여는 GalN에 대한 급성 간독성에 대해 보호효과를 갖으며, 양성 대조군인 silymarin 150 ㎎/㎏/day 투여와 비교하여 유사한 간독성 보호 활성을 갖는 것으로 나타났다. ALP의 경우 발효울금을 100 ㎎/㎏/day로 투여한 군에서만 GalN만을 투여한 군과 통계적으로 유의한 감소를 나타났고 300 ㎎/㎏/day 발효울금을 투여한 군과 silymarin 투여군은 통계적 유의차가 나타나지 않았다. 중성지방의 경우 발효울금 300 ㎎/㎏/day 투여군만 GalN만을 투여한 군과 비교하여 통계적 유의차가 나타났으나 silymarin 투여군에서는 동일한 효과가 나타나지 않았다. GalN으로 유도된 급성 간독성의 조직병리학적 결과 GalN만을 투여한 군에서 세포괴사 및 침윤된 염증세포, 카운실만 소체의 괴사가 심하게 나타났으며 300 ㎎/㎏/day 발효울금 투여군과 150 ㎎/㎏/day silymarin 투여군에서 간조직의 괴사 및 염증의 개선효과를 관찰할 수 있었다. 혈청 분석 및 조직병리학적 결과를 종합한 결과 GalN 유발 간독성에 대한 개선효과는 300 ㎎/㎏/day 발효울금 투여군과 150 ㎎/㎏/day silymarin 투여군에서 나타나는 것으로 판단할 수 있었다. 이러한 결과로부터 본 연구에서 사용한 Aspergillus oryzae로 발효하여 산업적 규격에서 생산된 발효울금은 간기능 저하 개선 기능을 갖는 새로운 기능성 소재로서 간질환과 관련된 다양한 기능성식품 개발에 이용될 수 있을 것으로 생각된다. In this study, the general components and minerals of fermented Curcuma longaL. (FC) by Aspergillus oryzae were examined as well as the hepatoprotective effects of FC on acute hepatotoxicity induced by a single dose of galactosamine (GalN, 650 ㎎/㎏ body weight (b.w.)). The FC was found to consist of 0.15% moisture, 4.68% crude fat, 4.35% crude protein, 6.92% crude fiber, and 6.83% crude ash. The P, Ca, and Mg levels in FC were also quantitatively analyzed. Male Sprague-Dawley rats were divided into six groups; nontreated control, GalN, 150 ㎎/㎏ b.w. of silymarin plus GalN, 30 ㎎/㎏ b.w. of FC plus GalN, 100 ㎎/㎏ b.w. of FC plus GalN, and 300 ㎎/㎏ b.w. of FC plus GalN. Pretreatment 300 ㎎/㎏ b.w. of FC during 14 days significantly decreased the increased in aspartate aminotransferase, alanine amino transferase, and triglyceride (TG) induced by GalN. Severe liver damage, hepatocellular necrosis, infiltration of inflammatory cells, and councilman body necrosis on histopathological liver tissues were observed in GalN treated rats. Administration of 300 ㎎/㎏ b.w. of FC significantly decreased the degree of live damage. These results suggest that FC displays hepatoprotective activity and FC was able to lower the TG levels in serum; thus, FC may serve as a useful material for health food and clinical conditions associated with liver disease.

A Serological Study of Hepatitis E Virus Infections in Korea

Lee, Kyu Pum,Choi, Cheel Seok,Lee, Mu Ju,Lee Kyung Ok 대한임상병리학회 1992 대한임상병리학회지 Vol.12 No.4

An Electrochemical Biosensor Array for Rapid Detection of Alanine Aminotransferase and Aspartate Aminotransferase

SONG, Min-Jung,YUN, Dong-Hwa,HONG, Suk-In Japan Society for Bioscience, Biotechnology, and A 2009 Bioscience, Biotechnology, and Biochemistry Vol.73 No.3

<P>An increment of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) in human serum indicates an abnormal symptom of the liver. Hence, an electrochemical biosensor array that uses micro electro mechanical systems technology is required for rapid and integrated measurement of ALT/AST. Here we describe a biosensor array consisting of two glutamate sensors. It turned out that porous silicon layers formed on each working electrode were useful to increase the effective surface area. This biosensor array was constructed with platinum electrodes and a polydimethylsiloxane (PDMS) microchannel. Electrodes in sampling wells minimized a cross-interference effect and permitted multiple sampling by immobilization with glutamate oxidase using a silanization technique. The device sensitivities derived from semi-logarithmic plots were 0.145 μA/(U/l) for ALT and 0.463 μA/(U/l) for AST over a range of 1.3 U/l to 250 U/l. Hene, this ALT/AST biosensor array can be applied in diagnostic and home use.</P>

Analysis of Clinical Biochemical Components in Sera of Tsutsugamushi Disease Patients

Chong-Ho Kim,Seung-Taeck Park,Geum-Ga Oh 대한의생명과학회 2007 Biomedical Science Letters Vol.13 No.4

The factors and mechanisms by infection of Oriental Tsutsugamushi caused disease are not well understood. The onset of tsutsugamushi disease is characterized by chilliness, fever, malaise, headache and generalized aching. Infection of tsutsugamushi is the cause of impairment of function of a major organ often complicate the picture and immediately change the prognosis for the worse. Tsutsugamushi disease is reported that this disease is characterized by the histopathogenesis of liver, kidney, heart, and lung, but the variation of biochemical components in serum of tsutsugamushi disease patient are not clear. We analyzed total protein (TP), albumin (AL), aspartic aminotranferase (AST), alanine aminotransferase (ALT), alkaline phosphotase (ALP), urea nitrogen (UN), creatinine (CRE), glucose (GLU), cholesterol (CHOL) and total bilirubin (TB) in sera of patients with tsutsugamushi disease. In comparison with reference, total protein and albumin were abnormally decreased in 19.6% and 39.2% of patients, respectively. AST, ALT, ALP, creatinine, UN, glucose, cholesterol and total bilirubin were abnormally increased in 94.1%, 72.5%, 25.5%, 15.7%, 9.8%, 62.7%, 25.5% and 6.0% of patients, respectively. The patients showed abnormal relative rate of protein electrophoretic fractions to total protein in serum compared to them of reference were 43.1% (albumin), 12.9% (α1-globulin), 58.8% (α2-globulin), 60.8% (β-globulin) and 70.6% (γ-globulin), respectively. These data suggest that infection of Oriental Tsutsugamushi causes impairment of function of a major organ and abnormal serum protein electrophoresis fractions to tsutsugamushi patients.

Protein Electrophoresis Fraction in Serum of the Rural Elderly Patient

Kim Chong-Ho,Park Chung-Oh,Kang Young-Tae,Park Seung-Taeck The Korean Society for Biomedical Laboratory Scien 2006 Journal of biomedical laboratory sciences Vol.12 No.3

We analyzed total protein (TP), albumin (AL), aspartic aminotransferase (AST), alanine aminotransferase (ALT), urea nitrogen (UN), creatinine (CRE) and serum protein electrophoretic fractions in sera of rural elderly patients to evaluate the health status in rural elderly patients. We observed that the frequencies of patients showed lower level of total protein, albumin, and both total protein and albumin than them of reference range were 20.3%, 22.8% and 19.0%, respectively. The rates of patients showed higher level of AST, ALT, both of AST and ALT, UN, creatinine and both of UN and creatinine than them of reference range were 33.8%, 40.0%, 30.0%, 17.7%, 15.2% and 7.9%, respectively. Comparison of protein fractions of each patient to reference range showed that 77.2% of patients showed normal in all of patterns. Few of patients showed abnormal pattern in albumin (13.6%), ${\alpha}1-globulin (0.0%),\;{\alpha}_2-g10bulin(1.1%),\;{\beta}-globulin(1.9%)\;and\;{\gamma}-globulin(6.2%)$. These data suggest that many of rural elderly patients may suffer from heart, liver and kidney diseases. The serum protein fractions are not typical criterion to evaluate the disease, but production of proteins in rural elderly patients may be affected by liver disease and kidney disease.

흰쥐의 알코올 유발성 간손상에 실비음(實脾飮)이 미치는 보호 효과

김범회 한방비만학회 2018 한방비만학회지 Vol.18 No.2

Objectives: The objective of this study is to investigate the effects of Silbi-um (SBU) extract on the alcoholic fatty liver induced by EtOH administration for 8 weeks. Methods: Male Sprague Dawley rats were used. All animals were randomly divided into 3 groups; Normal, EtOH and EtOH+SBU. The rats of EtOH group were daily treated with ethanol of 25% (v/v) for 8 weeks (n=10). EtOH+SBU group was orally treated with SBU water extract after ethanol administration (n=10). The rats of Normal group were treated with saline (n=10). After 8 weeks, the mean body weight, liver weight, and liver-body weight ratio were calculated. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of all groups were measured. The morphological alterations were observed using hematoxylin and eosin (H&E) and Oil Red O staining. Moreover, the alteration of tumor necrosis factor-α (TNF-α) levels were analyzed immunohistochemistrically. Results: The histological data showed that liver sections from EtOH group displayed severe steatosis. SBU extract significantly inhibited the progression of the alcoholic liver injury. The increased serum level of ALT and AST induced by ethanol administration were decreased by SBU extract. Furthermore, SBU extract significantly decreased the liver concentrations of TNF-α. Conclusions: SBU water extract attenuated the alcohol induced fatty liver by improving hepatic lipid metabolism via suppression of TNF-α protein. SBU could be effective in protecting the liver from alcoholic fatty liver.