Resistance to ACCase Inhibitor Cyhalofop-butyl in Echinochloa crus-galli var. crus-galli Collected in Seosan, Korea

Su-Hyeon Im(임수현),Min-Won Park(박민원),Min-Jeong Yook(육민정),Do-Soon Kim(김도순) 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.2

2007년도 충청남도 서산 현대간척지의 6개 논 포장에서 수집한 물피의 ACCase 저해제인 cyhalofop-butyl에 대한 저항성 여부를 평가하기 위하여 본 연구를 수행하였다. 6개 수집종들의 cyhalofop-butyl에 대한 약량반응 실험을 통해 얻어진 GR50값을 감수성의 값과 비교하여 얻어진 저항성 지수인 R/S 비율을 검토한 결과 3개의 수집종에서 R/S 비율이 30 이상으로 평가되어 cyhalofop-butyl에 저항성이 있음이 확인되었다. 특히 서산-5로 명명된 수집종의 경우 R/S 비율이 96.9로 저항성이 매우 높은 것으로 판단되었으며 화학구조적으로 유사한 AOPP 계열의 fenoxaprop-P-ethyl과 metamifop에 대해서도 교차저항성이 있음이 확인되었다. ACCase 저해제가 아닌 다른 작용기작을 갖는 대체 제초제를 선발하기 위하여 ALS 저해제에 대해 평가한 결과 pyribenzoxim이 저항성인 서산-5를 비교적 양호하게 방제하는 것으로 확인되었다. 본 연구결과는 한국에서 ACCase 저해제 저항성을 확인한 첫 보고로서 향후 저항성 기작과 저항성 물피의 시공간적 확산 등에 관한 연구에 활용될 수 있을 것으로 판단된다. Six biotypes of Echinochloa crus-galli var. crus-galli were collected from Seosan Hyundai farm, Seosan, Chungnam, Korea, in 2007 and tested with cyhalofop-butyl, to confirm if any of them are resistant to acetyl CoA carboxylase (ACCase) inhibitors. Three biotypes showed strong resistance to cyhalofop-butyl with R/S ratios of greater than 30. Seosan-5 showed the greatest R/S ratio, 96.9, and was also confirmed to have cross-resistance to other ACCase inhibiting aryloxyphenoxy propionate herbicides, fenoxaprop-P-ethyl and metamifop. As an alternative herbicide with a different mode of action, ALS inhibitor pyribenzoxim showed relatively good efficacy against resistant biotype Seosan-5 as compared with cyhalofop-butyl and penosxulam. Our results are the first report of ACCase inhibitor resistance in Korea, and thus extensive further works are required to investigate resistance mechanisms and spatio-temporal dispersal of this reistance in Seosan and other rice paddy fields in Korea where ACCase inhibitors have intensively been used for Echinochloa control.

ACCase 및 ALS 저해 제초제 저항성 강피의 엽기별 약제방제효과

이인용(In Yong Lee),권오도(Oh Do Kwon),김창석(Chang Seok Kim),이정란(Jeong Ran Lee),신해룡(Hae Ryoung Shin),문병철(Byung Chul Moon),박재읍(Jae Eup Park),국용인(Yong In Kuk) 韓國雜草學會 2011 Weed&Turfgrass Science Vol.31 No.2

제초제 저항성 피의 종합방제를 위해 약제별, 엽기별 방제효과를 확인하였다. 저항성 강피 발생전 토양처리 제초제의 효과는 옥사디아길 1.7% 유제, 옥사디아존 12% 유제, 펜트라자마이드·옥사디아길 3.3 % 유제의 3종에서 100% 방제되었으며, 펜톡사존 5% 액상수화제는 약제처리 후 31일부터 후발 피가 발생되기도 하였으나 98% 이상 우수한 효과를 보였다. 저항성 피 1.0~2.5엽까지 100% 방제가 가능한 제초제는 아짐설퓨론·카펜스트롤 1.05% 입제, 벤설퓨론메틸·벤조비사이클론·메페나셋 24.52% 액상화수화제, 벤셀퓨론메틸, 펜트라자마이드 7% 액상수화제, 벤셀퓨론메틸·메페나셋·옥사디아길 21.6% 액상수화제, 메페나셋·피라조설퓨론에틸 3.57% 입제 등 5종이었다. 피 3.0엽기까지 방제가 가능한 제초제는 벤조비사이클론·메페나셋·페녹슐람 21.5% 액상수화제와 메페나셋·피라조설퓨론에틸 3.57% 입제 등 2종이었다. ACCase 및 ALS 제초제 저항성 피를 완벽하게 관리하기 위해서는 발아전 제초제와 초·중기 토양처리제초제의 선택과 이들 제초제의 체계처리가 중요할 것으로 판단된다. Weeding effect was investigated based on the leaf stages to several different herbicide treatments for an integrated weed management of herbicide resistant Echinochloa oryzoides to ACCase and ALS inhibitors in a rice field. Efficacy of soil-applied herbicide treatments before resistant E. oryzoides occurred was very effective. Pentaxazon 5% SC showed over 98% of weeding effect although E. oryzoides were emerged 31 days after the treatment. Until the leaf stage of 2.5, five herbicides, azimsulfuron·carfenstole 1.05% GR, bensulfuron-methyl·benzobicyclone· mefenacet 24.52% SC, bensulfuron-methyl·fentrazamide 7% SC, bensulfuron-methyl·mefenacet· oxadiargyl 21.6% SC and mefenacet·pyrazosulfuron-ethyl 3.57% GR showed perfect weeding effect. Benzobicyclone·mefenacet·penoxulam 21.5% SC and mefenacet·pyrazosulfuron-ethyl 3.57% GR were effective at the leaf stage of 3.0. It is very important to select the right herbicides for timing and their systematic application for controlling of E. oryzoides resistant to ACCase-and ALS-inhibitors.

ACCase계 저해제 저항성 피의 cyhalofop-butyl 약량별 반응 및 검정법

문병철(Byeong-Chul Moon),박재읍(Jae-Eup Park),이인용(In-Yong Lee),박태선(Tae-Seon Park),박종민(Jong-Min Park),김도순(Do-Soon Kim),진용덕(Yong-Duk Jin),권혜영(Hye-Yong Kwon),임건재(Geon-Jae Im) 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.4

본 시험은 서산 및 수원지역에서 수집한 물피를 이용해서 저항성 여부를 조기에 확인하기 위한 검정법확립을 위해 수행하였다. Cyhalofop-butyl 유제의 처리약량이 증가할수록 수집 생태형별 신초 재생양상은 다른 경향을 보였다. 약량별 신초재생의 차이는 ha당 유효성분량 25g 처리에서 절단 12일 후, 50g 처리이후는 절단 후 3일부터 크게 다른 경향을 보였다. 유효성분량 100g 처리까지는 수원 수집종에서도 이후 시간경과에 따라서 점차 신초가 재생되는 경향을 보였으나 200g 처리 이후부터 재생이 현저하게 억제되었으며 400g 처리에서는 거의 재생되지 않았다. 저항성(R)-물피 4엽기 및 5엽기에 대한 cyhalofop-butyl 유체의 생체중 50% 억제농도(GR50)는 ha당 유효성 분량이 각각 158.1g, 264.2g 이었으며 4, 5엽기별 감수성생태형 50%억제농도에 대한 저항성비(R/S값)는 각각 3.4, 5.7배의 저항성을 보였다. ACCase 저항성피를 효율적으로 관리하기 위해서는 저항성을 조기에 검지할 수 있는 간이 신속검정법 확립이 매우 중요하기 때문에 제초제 처리 24시간 후 지제부로부터 3cm 위 줄기부위를 절단하고 신초 재생여부에 따라서 저항성을 판정할 수 있는 방법을 구명하였다. Two biotypes of E crus-galli var crus-galli were collected from Seosan Hyundai farm, Seosan (resistant biotype) and Suwon (susceptible biotype) in 2007 and tested with cyhalofop-butyl, to establish method diagnosing rapidly if E. crus-galli seeds corrected were resistant to cyhalofop-butyl EC. The results are summarized as follows. Germination of new shoot in the a part cut 24 hours after treatment with cyhalofop-butyl EC was responded differently by biotypes of E. crus-galli collected. In case of Suwon biotypes, the shoot in the cutting parts was not almost germinated in the treatment above standard amount whereas it was germinated from 3days after cutting in most treatment of Seosan biotypes (R). R/S ratios by dose-responses of Seosan (R) and Suwon biotypes (S) in the lower cutting parts after treatment with cyhalofop-butyl EC showed values of 3.4 and 5.7 at 4 and 5 leaf stage, respectively. Accordingly, judgement by response of shoot in the parts cutting 24 hours after treatment was considered to be effective as method to diagnose rapidly if E. crus-galli were resistant to cyhalofop-butyl.

Fenoxaprop-P-ethyl의 제초활성에 대한 Bentazon의 길항작용기구

마상용,김승우,전재철,Ma, S.Y.,Kim, S.W.,Chun, J.C. 한국잡초학회 1998 Weed&Turfgrass Science Vol.18 No.2

Fenoxaprop-P-ethyl에 대한 bentazon의 길항작용기작을 검정하기 위하여 4엽기의 벼와 피를 대상으로 bentazon 혼합처리에서 나타나는 fenoxaprop-P-ethyl의 흡수, 이행, 대사 및 작용점의 활성 변화를 조사하였다. Fenoxaprop-P-ethyl에 대하여 벼와 피의 지상부에서 생육억제가 크게 나타났다. Fenoxaprop-P-ethyl 단독처리에 있어서 두 초종 모두에서 처리 직후부터 빠른 속도로 약제가 흡수되어, 총 흡수량의 90%이상이 약제처리 6시간 이내에 이루어졌으며, 처리 24시간까지 흡수량의 약 30%가 처리엽의 상, 하위조직으로 이행되었다. Ethyl ester형으로 처리된 약제는 신속히 acid형으로 전환되었으며, 처리 24시간 후부터는 acid형이 conjugate형의 대사물로 전환되었다. 그러나 bentazon에 의한 fenoxaprop-P-ethyl의 흡수, 이행 및 대사활성의 변화는 인정되지 않았다. In vitro ACCase의 활성은 벼와 피 에서 각각 26.5 및 23.2nmol/min/mg protein으로 나타났다. 이 효소 활성의 50% 저해농도에 있어서 fenoxaprop-P-ethyl 단독처리와 bentazon 혼합처리 사이에 유의성은 인정되지 않았다. 그러나 bentazon 처리에 의하여 in vivo ACCase 활성의 감소와 엽록체 thylakoid에서 전자전달의 억제가 나타났다. 따라서 fenoxaprop-P-ethyl에 대한 bentazon의 길항작용기작은 bentazon이 fenoxaprop-P-ethyl 작용점의 활성에 직접적으로 관여하는 것이 아니라, 엽록체 구조 및 활성 저해에 의한 fenoxaprop-P-ethyl의 작용점 감소를 통하여 간접적으로 fenoxaprop-P-ethyl의 활성을 억제하는 것으로 판단된다. Antagonistic mode of action of fenoxaprop-P-ethyl [ethyl(R)2-4-{(6-chloro-2-benzoxazolyloxy) phenoxy}propionate] with bentazon was investigated with respect to absorption, translocation, metabolism, and change in target site response of fenoxaprop-P-ethyl using four-leaf stage of rice(Oryza sativa L.) and barnyardgrass [Echinochloa eras-galli (L.) P. Beauv.]. Shoots of rice and barnyardgrass was more sensitive to fenoxaprop-P-ethyl than the roots. More than 90% of fenoxaprop-P-ethyl was absorbed within 6 hours after treatment and 30% of the absorbed was acropetally and basipetally translocated at 24 hours after treatment. Fenoxaprop-P-ethyl was rapidly transformed to its acid form, fenoxaprop(2-[4-(6-chloro-2-benzoxazolyloxy)phenoxy]propionic acid), which was subsequently metabolized to polar conjugates. However, changes in absorption, translocation, and metabolism of fenoxaprop-P-ethyl by bentazon treatment were not found in both species. Background activity of acetyl-CoA carboxylase(ACCase) in rice and barnyardgrass was 26.5 and 23.2nmol/min/mg, respectively. Concentration required to inhibit fifty percent enzyme activity$(I_{50})$ in vitro was 6.5~7.4${\mu}M$ of fenoxaprop-P-ethyl and more than 500${\mu}M$ of bentazon. There were no significant differences in $I_{50}$ value between two treatments of fenoxaprop-P-ethyl alone and its bentazon mixture. However, bentazon reduced ACCase activity in vivo and inhibited electron transport in chloroplast thylakoid. Based on the results obtained, it is concluded that the antagonistic effect of bentazon occurs due not to direct effect on target site of fenoxaprop-P-ethyl, but to indirect involvement in reducing herbicidal activity of fenoxaprop-P-ethyl through physiological disturbances caused by bentazone at whole chloroplast level.

ACCase 및 ALS 저해 제초제들에 대한 저항성 강피의 반응과 대체약제들의 효과

박태선(Tae-Seon Park),구본일(Bon-Il Ku),강신구(Sin-Koo Kang),최민규(Min-Kyu Choi),박홍규(Hong-Kyu Park),이경보(Kyong-Bo Lee),고재권(Jae-Kwon Ko) 韓國雜草學會 2010 Weed&Turfgrass Science Vol.30 No.3

ACCase 저해해제인 cyhalofop-butyl과 ALS 저해제인 penoxsulam은 한국의 직파재배 논에서 수년 동안 경엽 처리용으로 사용되어지고 있다. 그러나 2009년 이후 익산, 김제, 부안에서 이들 제초제를 사용한 벼 직파재배 논에서 강피 약효에 대한 민원이 증가하고 있다. ACCase 및 ALS 저해제 저항성으로 추정되는 강피를 3개 지역에서 수집하여 온실조건에서 cyhalofop-butyl과 penoxsulam에 대한 저항성 정도를 검증하였다. 익산과 김제의 수집종은 cyhalofop-butyl과 penoxsulam의 추천량에서 완전 생존하였으며, 부안 수집종은 약 60% 생존하였다. 온실조건에서 화본과 잡초인 피를 방제를 위한 ACCase 및 ALS 저해제들 이 혼합된 제초제들을 강피 3.5엽기에 처리한 결과 무처리 대비 생존율은 80% 이상으로 나타났다. 파종 및 이앙전 처리제들인 oxadiazon EC, pyrazolate SC, pretilachlor EC, benzobicyclon+thiobencarb SE들은 강피 0.5엽기에서 효과적이었으며, benzobicyclon+fentrazamide+bensulfuron SC, benzobicyclon+mefenacet+bensulfuron SC 그리고 benzobicyclone+cafenstrole+pyrazosulfuron-ethyl GR은 강피 2엽기까지 효과적으로 방제하였다. 포장실험에서 benzobicyclon+fentrazamide+bensulfuron SC과 benzobicyclon+mefenacet+bensulfuron SC의 단일처리에서는 저항성 강피의 방제에 실패하였으나, benzobicyclon+thiobencarb SE와 benzobicyclon+mefenacet+bensulfuron SC의 체계처리는 강피를 효과적으로 방제하였다. 따라서 강피의 익산, 김제 수집종은 cyhalofop-butyl과 penoxsulam에 대하여 강한 저항성을 보였으나, 작용기작이 다른 cafenstrole, fentrazamibe, mefenacet에 의해 2엽기 이내에는 효과적으로 방제되었다. Cybalofop-butyl and penoxsulam, known for respective acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor, have been widely using as a post-emergence foliar application for many years in wet-seeded rice field in Korea. Since 2009, control of Echinochloa oryzoides with cyhalofop-butyl and penoxsulam was no longer satisfactory. Greenhouse and experiment in rice field were conducted to confirm E. oryzoides resistance to ACCase and ALS inhibitors and to compare herbicide treatments for control of the resistant E. oryzoides to ACCase and ALS inhibitors. Three resistant E. oryzoides to ACCase and ALS inhibitors accessions were tested for levels of resistance to cyhalofop-butyl and penoxsulam, base on survival rate. Iksan and Kimje accessions were not any affected to the survival by treatment with recommended doses of two herbicides tested. Buan accession displayed an intermediate response of 62 and 72% survival at recommended doses of cyhalofop-butyl and penoxsulam, respectively. Survival rates to herbicides mixed with ACCase and ALS inhibitors at 3.5 leaf stage of E. oryzoides was over 80%. Benzobicyclon+fentrazamide+bensulfuron SC, benzobicyclon+mefenacet+bensulfuron SC, benzobicyclone-cafenstrole+pyrazosulfuron-ethyl GR controlled effectively by 2 leaf stage of resistant E. oryzoides to ACCase and ALS inhibitors. In the field experiment, single treatment of benzobicyclon+ fentrazamide+bensulfuron SC and benzobicyclon+mefenacet+bensulfuron SC failed to control E. oryzoides, but squential treatment of benzobicyclon+thiobencarb SE and benzobicyclon+ mefenacet+bensulfuron SC controlled effectively it in rice infant seedling culture with machine. Our results suggest that resistant E. oryzoides to ACCase and ALS inhibitors had not developed multiple resistance to herbicides with different modes of action. In particular, cafenstrole, fentrazamide, mefenacet were effective control measures.

Identification of Herbicide-Resistant Barnyardgrass (Echinochloa crus-galli var. crus-galli) Biotypes in Korea

Won, Ok Jae,Lee, Jeung Joo,Eom, Min Yong,Suh, Su Jeoung,Park, Su Hyuk,Hwang, Ki Seon,Pyon, Jong Yeong,Park, Kee Woong The Korean Society of Weed ScienceThe Turfgrass So 2014 Weed & Turfgrass Science Vol.3 No.2

The continuous use of acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors has led to the selection of herbicide resistant barnyardgrass populations in direct-seeded rice fields of Korea. This study was conducted to identify herbicide resistant barnyardgrass biotypes and to determine the cross- and multiple-resistance of them. 25% of the population collected from Taeahn was partially resistant to ACCase inhibitors and 22% collected from Kimjae were partially resistant to ALS inhibitors. However, 8.2% of the population from both sites was resistant to ALS and ACCase inhibitors. Resistance to sulfonylurea herbicide, flazasulfuron was identified from two barnyardgrass accessions collected from both Taeahn and Kimjae. One barnyardgrass accession from both sites was resistant to ACCase inhibitor, sethoxydim. The cross-resistance to ALS inhibitors was identified at one barnyardgrass accession from Taeahn and at two accessions from Kimjae. Further, crossresistance to ACCase inhibitors was also identified at barnyardgrass accessions from Taeahn and Kimjae. Multiple-resistance to flazasulfuron and sethoxydim was determined at four barnyardgrass accessions from Taeahn and at six accessions from Kimjae. Therefore, the herbicide mixture and sequences within a growing season or the herbicide rotation with different modes of actions across growing seasons are recommended to control herbicide-resistant barnyardgrass in infested fields.

Identification of Herbicide-Resistant Barnyardgrass (Echinochloa crus-galli var. crus-galli) Biotypes in Korea

원옥재,이증주,엄민용,서수정,박수혁,황기선,편종영,박기웅 한국잔디학회 2014 Weed & Turfgrass Science Vol.3 No.2

The continuous use of acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors has led tothe selection of herbicide resistant barnyardgrass populations in direct-seeded rice fields of Korea. This study was conducted toidentify herbicide resistant barnyardgrass biotypes and to determine the cross- and multiple-resistance of them. 25% of thepopulation collected from Taeahn was partially resistant to ACCase inhibitors and 22% collected from Kimjae were partiallyresistant to ALS inhibitors. However, 8.2% of the population from both sites was resistant to ALS and ACCase inhibitors. Resistance to sulfonylurea herbicide, flazasulfuron was identified from two barnyardgrass accessions collected from both Taeahnand Kimjae. One barnyardgrass accession from both sites was resistant to ACCase inhibitor, sethoxydim. The cross-resistance toALS inhibitors was identified at one barnyardgrass accession from Taeahn and at two accessions from Kimjae. Further, crossresistanceto ACCase inhibitors was also identified at barnyardgrass accessions from Taeahn and Kimjae. Multiple-resistance toflazasulfuron and sethoxydim was determined at four barnyardgrass accessions from Taeahn and at six accessions from Kimjae. Therefore, the herbicide mixture and sequences within a growing season or the herbicide rotation with different modes of actionsacross growing seasons are recommended to control herbicide-resistant barnyardgrass in infested fields.

Identification of Herbicide-Resistant Barnyardgrass (Echinochloa crus-galli var. crus-galli) Biotypes in Korea

Ok Jae Won,Jeung Joo Lee,Min Yong Eom,Su Jeoung Suh,Su Hyuk Park,Ki Seon Hwang,Jong Yeong Pyon,Kee Woong Park 한국잡초학회·한국잔디학회 2014 Weed & Turfgrass Science Vol.3 No.2

The continuous use of acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors has led to the selection of herbicide resistant barnyardgrass populations in direct-seeded rice fields of Korea. This study was conducted to identify herbicide resistant barnyardgrass biotypes and to determine the cross- and multiple-resistance of them. 25% of the population collected from Taeahn was partially resistant to ACCase inhibitors and 22% collected from Kimjae were partially resistant to ALS inhibitors. However, 8.2% of the population from both sites was resistant to ALS and ACCase inhibitors. Resistance to sulfonylurea herbicide, flazasulfuron was identified from two barnyardgrass accessions collected from both Taeahn and Kimjae. One barnyardgrass accession from both sites was resistant to ACCase inhibitor, sethoxydim. The cross-resistance to ALS inhibitors was identified at one barnyardgrass accession from Taeahn and at two accessions from Kimjae. Further, crossresistance to ACCase inhibitors was also identified at barnyardgrass accessions from Taeahn and Kimjae. Multiple-resistance to flazasulfuron and sethoxydim was determined at four barnyardgrass accessions from Taeahn and at six accessions from Kimjae. Therefore, the herbicide mixture and sequences within a growing season or the herbicide rotation with different modes of actions across growing seasons are recommended to control herbicide-resistant barnyardgrass in infested fields.

제초제 저항성 및 난방제 잡초 방제를 위한 제초제 선발

박태선,이인용,성기영,조현숙,김미향,양운호,서명철,강항원 한국잔디학회 2013 Weed & Turfgrass Science Vol.2 No.3

This study was conducted to select alternative herbicides to control herbicide-resistant and - troublesome weeds in rice fields, Korea. The resistant Echinochloa oryzicola to ACCase inhibitor was tested by herbicides registered to control Echinochloa oryzicola. And the resistant and troublesome weeds to sulfonylurea (SU) herbicides, ALS inhibitors, were tested by widely using herbicides to control the resistant weeds in Korea. The oxadiazon, pyrazolate, pretilachlor and benzobicyclone+thiobencarb effectively controlled resistant Echinochloa oryzicola to ACCase and ALS inhibitors at 0.5 leaf stage of the, Herbicides containing mefenacet or fentrazamide controlled effectively by the 2 leaf stage. In controlling other SU resistant weeds including Sagittaria trifolia and Ludwigia prostrate, benzobicyclon and mesotrione performed well all tested weeds, and carfentrazone and pytazolate were effective to broadleaf weeds. Sagittaria trifolia and Ludwigia prostrate, which were suspected to be resistance to SU herbicides, were well controlled by carfentrazone and pytazolate. 본 실험은 국내 논에서 우점하고 있는 주요 제초제 저항성 잡초들과 저항성잡초 가능성이 매우 높은 잡초들의 초종별 방제가능 제초제들을 설정하기 위하여 실시하였다.ACCase 및 ALS 저해제들에 대한 저항성 강피 방제에는 이앙전 처리제인 oxadiazon, pyrazolate, pretilachlor, benzobicyclone+thiobencarb들은 강피 0.5엽기에서 효과적이었으며,benzobicyclon+fentrazamide+bensulfuron, benzobicyclon +mefenacet+bensulfuron와 benzobicyclone+cafenstrole+pyrazosulfuron-ethyl은 강피 2엽기까지 효과적으로 방제되었다. 국내 논에서 발생하고 있는 주요 SU계 제초제 8 초종들에 대한 대체약제들의 반응은 benzobicyclon과mesotrione은 방동사니과 및 광엽잡초, bromobutide는 매자기를 제외한 방동사니과, carfentrazone과 pyrazolate는 광엽잡초들에 각각 효과적이었다. SU계 제초들에 대하여 저항성 가능성이 높은 다년생 벗풀에는 carfentrazone과pyraclonil, 그리고 일년생 여뀌바늘에는 carfentrazone,pyraclonil과 mesotrione이 효과적이었다.

Acetyl-CoA Carboxylase에 대한 2-(4-(6-chloro-2-benzoxazolyl)oxy)phenoxy-N-phenylpropionamide 유도체들의 분자 도킹과 제초활성

최원석(Won-Seok Choi),성낙도(Nack-Do Sung) 한국농약과학회 2010 농약과학회지 Vol.14 No.3

To search the new potent herbicidal agents by receptor-based approach, the interactions between receptor and substrate molecules from molecular docking to acetyl-CoA carboxylase(PDB code: 3K8X) of 2-(4-(6-chloro-2-benzoxazolyl)oxy)phenoxy-N-phenylpropionamide analogues (1-38) as substrate molecules were performed and discussed quantitatively. The most of the substrate molecules were formated 2 H-bonds between carbonyl oxygen atom of the substrate molecules and the amino acid residues (Ala1627 and Ile1735) in binding site of acetyl-CoA carboxylase (ACCase). But, the substrate molecules such as R₁=Acetyl substituents (6 & P9) were formated 3 H-bonds between H-bond acceptors in the substrate molecules and the H-bond donors in three amino acid residues including the rest residue (Gly1998). Therefore, the inhibitory activity factors of the substrate molecules against ACCase are due to the H-bonding characters that will be able to apply to the optimization of herbicidal agents.