Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells

( Zhuo Wang ),( Dan Wang ),( Yan Li ),( Xiuli Zhang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

A Rapidly New-typed Detection of Norovirus Based on F0F1-ATPase Molecular Motor Biosensor

Zhuo Zhao,Jie Zhang,Mei-Ling Xu,Zhi-Peng Liu,Hua Wang,Ming Liu,Yan-Yan Yu,Li Sun,Hui Zhang,Hai-Yan Wu 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1

In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycinbiotin- probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

Analysis on Coexistence of Ultra Wideband with OFDM-Based Communication Systems

Zhuo Li,Weixia Zou,Bin Li,Zheng Zhou,Xiaojun Huang IEEE 2011 IEEE transactions on electromagnetic compatibility Vol.53 No.3

<P>Considering the potential interference of ultrawideband (UWB) on OFDM-based wireless communication systems, this paper studies coexistence issue between UWB with both impulse radio (IR) and multiband (MB) modulation and IEEE802.11n, WiMAX, and LTE downlink systems, using physical layer modeling strategy. According to the physical characteristics of UWB and the victim systems, entire physical layer models are set up. Based on the requirements of bit error rate (BER) and receiver sensitivity of the three victim systems, the emission limits of UWB are respectively obtained. Compared with a method based on interference to noise ratio (I/N) criteria, this method reflects the effect of the actual systems, so the conclusion can provide the reference to formulating the frequency spectrum of UWB within the operating frequency bands of the victim systems. Besides, we also reveal that IR-UWB should be more strictly regulated compared with MB-OFDM because of its time domain characteristic.</P>

The Navigated Oblique Lumbar Interbody Fusion: Accuracy Rate, Effect on Surgical Time, and Complications

Zhuo Xi,Dean Chou,Praveen V. Mummaneni,Shane Burch 대한척추신경외과학회 2020 Neurospine Vol.17 No.1

Objective: The oblique lumbar interbody fusion (OLIF) can be done with either fluoroscopy or navigation. However, it is unclear how navigation affects the overall flow of the procedure. We wished to report on the accuracy of this technique using navigation and on how navigation affects surgical time and complications. Methods: A retrospective review was undertaken to evaluate patients who underwent OLIF using spinal navigation at University of California San Francisco. Data collected were demographic variables, perioperative variables, and radiographic images. Postoperative lateral radiographs were analyzed for accuracy of cage placement. The disc space was divided into 4 quadrants from anterior to posterior, zone 1 being anterior, and zone 4 being posterior. The accuracy of cage placement was assessed by placement. Results: There were 214 patients who met the inclusion criteria. A total of 350 levels were instrumented from L1 to L5 using navigation. The mean follow-up time was 17.42 months. The mean surgical time was 211 minutes, and the average surgical time per level was 129.01 minutes. After radiographic analysis, 94.86% of cages were placed within quartiles 1 to 3. One patient (0.47%) underwent revision surgery because of suboptimal cage placement. For approach-related complications, transient neurological symptoms were 10.28%, there was no vascular injury. Conclusion: The use of navigation to perform OLIF from L1 to L5 resulted in a cage placement accuracy rate of 94.86% in 214 patients.

Size-dependent torsional deformation of silicon nanowires

Zhuo, Xiao Ru,Beom, Hyeon Gyu Elsevier 2018 Materials letters Vol.213 No.-

<P><B>Abstract</B></P> <P>The effect of size on the torsional deformation of [1 1 0]-oriented silicon nanowires is examined via molecular dynamics simulations. The plastic deformation mechanism of silicon nanowires depends on the nanowire diameter. Sub-5.7-nm-diameter silicon nanowires yield by means of a crystal-to-amorphous transition, which nucleates at the outer surface and propagates along the cross-section of the wires. On the other hand, perfect dislocations nucleate at the outer surface of wires whose diameter equals or exceeds 7.7 nm, initiating plastic deformation. These dislocations have a Burgers vector of 1/2 ⟨1 1 0⟩ and slip along the {1 1 1} planes. These findings may enhance our understanding of plasticity of silicon nanowires.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Torsional deformation of [1 1 0]-oriented silicon nanowires is size-dependent. </LI> <LI> Sub-5.7-nm-diameter silicon nanowires yield by a crystal-to-amorphous transition. </LI> <LI> Coaxial dislocations form in nanowires whose diameter equals or exceeds 7.7 nm. </LI> </UL> </P>

Examining Stock Volatility in the Segmented Chinese Stock Markets: A SWARCH Approach

ZHUO QIAO,WEIWEI QIAO,Wing-Keung Wong 연세대학교 동서문제연구원 2010 Global economic review Vol.39 No.3

This study adopts the SWARCH model to examine the volatile behavior and volatility linkages among the four major segmented Chinese stock indices.We find strong evidence of a regime shift in the volatility of the four markets, and the SWARCH model appears to outperform standard generalized autoregressive conditional heteroskedasticity (GARCH) family models. The evidence suggests that, compared with the A-share markets, B-share markets stay in a high-volatility state longer and are more volatile and shift more frequently between high- and low-volatility states. In addition, the relative magnitude of the high-volatility compared with that of the low-volatility state in the B-share markets is much greater than the case in the two A-share markets. B-share markets are found to be more sensitive to international shocks, while A-share markets seem immune to international spillovers of volatility. Finally, analyses of the volatility spillover effect among the four stock markets indicate that the A-share markets play a dominant role in volatility in Chinese stock markets.

A lightweight and flexible CNT/Fe3O4 composite with high electromagnetic interference shielding performance

Zhuo Chao,Yingying Yu,Fang Lei,Dongmei Hu 한국탄소학회 2021 Carbon Letters Vol.31 No.1

Lightweight and flexible electromagnetic interference (EMI) shielding materials are in great demand for wearable EMI device. In the present work, lightweight and flexible carbon nanotube (CNT)/ferroferric oxide ( Fe3O4) composite film was made through a feasible chemical vapor deposition process for CNT film synthesis, followed by a hydrothermal reduction process for Fe3O4 coating. In the as-prepared composite, CNT film and Fe3O4 particles work as conductive skeleton and strong magnetic particle, respectively. The as-prepared composite film shows a novel EMI shielding effectiveness (SE) of 91 dB in the X-band, a small thickness of 0.09 mm and a low density of 0.86 g/cm3, which is superior to most of the carbonbased EMI materials.

Suspension Footbridge Form-Finding with Laplacian Smoothing Algorithm

Zhuo-ju Huang,Jie-min Ding,Sheng-yi Xiang 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.6

In this paper, Laplacian smoothing, which is an algorithm originally used to smooth polygon meshes in computer graphics (CG), is applied to solve a structural form-fi nding problem with the proof that the result of such algorithm is equivalent to force density method. Such CG algorithm is used on the design of a new-built suspension footbridge in Shaoxing, China and the algorithm works well. Since Laplacian smoothing is a pure geometric algorithm without any mechanical concept, the algorithm shows the inner relationship between force and shape, more structural applicable CG algorithms are expected to be found in the future.

How High School Mathematics Teachers Use New Textbook : A Case Study from China

( Zhuo Li ),( Jiansheng Bao ) 한국수학교육학회 2023 수학교육연구 Vol.26 No.4

In this paper, we propose a theoretical framework for Chinese high school mathematics teachers use new textbooks based on the work of Remillard (1999) and Chau (2014). Based on this framework, a multiple case approach was used to investigate how two high school mathematics teachers from Shanghai use new textbooks. The results suggest that in the curriculum mapping arena, both the novice teacher and the expert teacher often planned to appropriate the unit content, and sometimes planned to add supplemental content. When organizing the unit content, novice teacher always planned to follow the new textbook in sequence, while expert teacher often would follow the new textbook in sequence, but sometimes planned to rearrange the unit content. In the design arena, both the novice teacher and the expert teacher tended to appropriate the introduced tasks and definitions. The novice teacher often planned to appropriate the example problems and exercise problems, while the expert teacher often intended to flexibly use the example problems and exercise problems. In the construction arena, the novice teacher seldom adjusted the planned tasks; in contrast, the expert teacher adjusted the planned tasks more frequently. In the reflection arena, the novice teacher often thought she should improve the mathematics tasks, while the expert teacher almost always thought he needed to improve the mathematics tasks. The framework shown in this paper provides a tool to investigate how mathematics teachers use textbooks.

Novel CHR-2 SINE subfamilies and t-SINEs identified in cetaceans using nonradioactive Southern blotting

Zhuo Chen,Guang Yang 한국유전학회 2010 Genes & Genomics Vol.32 No.4

The SINEs are short interspersed repetitive elements that propagate in eukaryotic genomes via transcription followed by reverse transcription. Recent genomic surveys have demonstrated that SINEs are active in shaping the architecture of genomes and therefore are powerful markers for studies of phylogeny and population biology. In the present study, a new protocol was developed to apply nonradioactive digoxigeninlabeling system into isolation of SINEs rapidly, safely, and efficiently. The application of this approach is illustrated with discovery of thirteen informative SINEs and a novel member of the t‐SINE family in the Omura’s whale (Balaenoptera omurai) and sperm whale (Physeter macrocephalus), which is the first record of t‐SINE in cetacean. Analysis of subfamilyspecific genetic variability and insertions/deletions strongly suggested the existence of some novel CHR‐2 SINE subfamilies in the cetacean genomes.