Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

Multiparametric Functional Magnetic Resonance Imaging for Evaluating Renal Allograft Injury

Yuan Meng Yu,Qian Qian Ni,Zhen Jane Wang,Meng Lin Chen,Long Jiang Zhang 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.6

Kidney transplantation is the treatment of choice for patients with end-stage renal disease, as it extends survival and increases quality of life in these patients. However, chronic allograft injury continues to be a major problem, and leads to eventual graft loss. Early detection of allograft injury is essential for guiding appropriate intervention to delay or prevent irreversible damage. Several advanced MRI techniques can offer some important information regarding functional changes such as perfusion, diffusion, structural complexity, as well as oxygenation and fibrosis. This review highlights the potential of multiparametric MRI for noninvasive and comprehensive assessment of renal allograft injury.

유치원 공간 구성에 환경색채연구 - 중국과 한국의 유치원 중심

원우몽 ( Yuan Yu Meng ),조성환 ( Jo Seong Hwan ) 한국기초조형학회 2019 한국기초조형학회 학술발표논문집 Vol.2019 No.1

COMPARISON ON HAN-LYU COSMETICS BUYING BEHAVIOR ACCORDING TO THE BRAND LEVEL BETWEEN KOREAN AND CHINESE FEMALE CONSUMERS

Jin-Hwa Lee,Yuan-Yang Bi,Meng Yu 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06

Towards the 21st century, the whole world is regarded as one market. Globalization is spreading all over the world as many cosmetics companies pioneering new market abroad, overcoming relatively limited domestic markets. Korean cosmetics companies are not the exception and they are aiming Chinese consumer market, the largest one in Asia, which is growing steadily since government’s open door policy. So, the necessity to compare and figure out the difference between Korean and Chinese consumer’s cosmetics buying behavior is very high for those companies that have their eyes on the Chinese Market. Therefore, the purpose of this study is to compare buying behavior between Korean and Chinese female consumers related to Korean cosmetics brand level. Cosmetics buying behavior is examined in 4 areas; purchasing motives, information sources, purchasing criteria, and the level of satisfaction. Subjects were selected through a convenient sample technique and a questionnaire was developed in Korean and Chinese by translation and back translation method. Data were collected from the sample of 187 out of 233 in Beijing for Chinese female consumers, and 188 out of 200 in Pusan for Korean female consumers. Respondents’ age varied from 20’s to 50’s. Korean cosmetics brands were divided into high /low levels; low brand level included Micha, The face shop, and Laneige while high brand level included Sulhwasu, O’hui, and The history of who. Among many types of cosmetics, this study focused on the basic types of cosmetics product such as lotion, moisture cream, nutrition cream, mask pack with the exception of color based cosmetics products. Data were analyzed by ANOVA and cluster analysis using SPSS 20.0. Respondents were divided into 4 groups: Korean high brand purchasing consumers, Korean Low brand purchasing consumers, Chinese high brand purchasing consumers, and Chinese low brand purchasing consumers. 1. For purchase motives, Chinese consumers affected by Korean celebrities and had more motives to experience Korean cosmetics. To relax and to relieve from stress was the main reasons to buy Korean cosmetics for Chinese high brand consumers. Price was the main reason to buy Korean cosmetics for both low brand purchasing groups. 2. For information sources, Chinese consumers had tendency to collect information from personal relations such as friends and family. Korean and Chinese low brand consumers used internet advertising. Chinese high brand consumers collected information from department salesman. Korean high brand consumers showed lowest use of any kinds of information sources. 3. Korean high brand group put on importance on color and scent as cosmetics purchase criteria. Chinese high brand consumers showed importance on brand image, ingredients, containers/packing, skin fit, and feeling. Korean and Chinese low brand groups have importance on brand image, ingredients, and skin fit. 4. Concerned satisfaction level, Korean high brand group showed lowest satisfaction level. Chinese consumers had satisfied with container design and feelings.

Structural and functional properties of Maillard-reacted casein phosphopeptides with different carbohydrates

Meng Yuan,Yu Cao,Haoyang Zheng,Kunlin Chen,Yuping Lu,Jing Wang,Liqin Zhu,Ming Chen,Zhipeng Cai,Yonggen Shen 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.7

This study used glucose, fructose, maltose and dextran to explore the effects of different carbohydrates on the Maillard reaction of casein phosphopeptides (CPP). The color parameter results showed that heating time from 1 to 5 h led to brown color, which was consistent with the observed increased in browning intensity. Fourier transform infrared spectroscopy results verified that four carbohydrates reacted with CPP to produce Maillard conjugates. Fluorescence spectroscopy showed that the Maillard reaction changed the tertiary structure of CPP by decreasing the intrinsic fluorescence intensity and surface hydrophobicity compared with the CPP-carbohydrate mixture. At the same time, the Maillard reaction effectively improved the emulsifying properties, reducing power and DPPH radical scavenging activity of CPP. Furthermore, this study also found that glucose and fructose improved CPP more than maltose and dextran. Therefore, monosaccharides have good potential in modifying CPP via the Maillard reaction.

DEVELOPMENT OF CHINA LIGHT-DUTY VEHICLE TEST CYCLE

Yu Liu,Zhi Xin Wu,Hua Zhou,Han Zheng Nan Yu,Xiao Pan An,Jing Yuan Li,Meng Liang Li 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.5

Driving cycles provide a basis for vehicle development and calibration and also serves as the foundation for energy consumption and emissions certification of vehicles. This paper presents the China Light-Duty Vehicle Test Cycle (CLTC) developed by the China Automotive Technology & Research Center (CATARC). First, the important steps and technical routes toward the CLTC development process are summarized. Second, the specific CLTC development process is presented in detail, including the data acquisition and data analysis procedures, weighting factor development and driving cycle construction. Then, the main driving characteristics of the New European Driving Cycle (NEDC), the Worldwide Harmonized Light-Duty Vehicles Test Cycle (WLTC), the Federal Test Procedure (FTP-75), the CLTC and the actual collected data are compared. The CLTC has low average speed, a high idle speed ratio and more frequent acceleration and deceleration characteristics. Finally, 70 vehicles are t ested based on the NEDC, WLTC, and CLTC according to their legislative procedures in the vehicle emission laboratories of the CATARC and the manufacturers. The results show that the CLTC’s fuel consumption is much higher than that of the NEDC and WLTC, and CLTC can effectively reflect the actual fuel consumption of users.

Study on the activation mechanism of lead ions in the flotation of ilmenite using benzyl hydroxamic acid as collector

Qingyou Meng,Zhitao Yuan,Li Yu,Yuankai Xu,Yusheng Du 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.62 No.-

The activation mechanism of Pb2+ ions to ilmenite and the subsequent interaction with benzyl hydroxamic acid (BHA) were systematically investigated. The Pb2+ ions modification occurred via a chemical adsorption process, in which lead species interacted with iron hydroxyl compounds to form lead-containing complexes on the ilmenite surface. After lead ions activation, the surface of ilmenite became more active, and iron species and lead-containing complexes served as the main active sites to covalently bond with BHA in the form of metal-BHA chelate complexes. As a result, the BHA adsorption increased, giving rise to a concomitant increase in the floatability of ilmenite.

Development of the vapor film thickness correlation in porous corrosion deposits on the cladding in PWR

Shen Yuan,Duan Zhengang,Lu Chuan,Ji Li,Jiao Caishan,Hou Hongguo,Chao Nan,Zhang Meng,Zhou Yu,Gao Yang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.12

The porous corrosion deposits (known as CRUD) adhered to the cladding have an important effect on the heat transfer from fuel rods to coolant in PWRs. The vapor film is the main constituent in the two-phase film boiling model. This paper presents a vapor film thickness correlation, associated with CRUD porosity, CRUD chimney density, CRUD particle size, CRUD thickness and heat flux. The dependences of the vapor film thickness on the various influential factors can be intuitively reflected from this vapor film thickness correlation. The temperature, pressure, and boric acid concentration distributions in CRUD can be well predicted using the two-phase film boiling model coupled with the vapor film thickness correlation. It suggests that the vapor thickness correlation can estimate the vapor film thickness more conveniently than the previously reported vapor thickness calculation methods.

In Vivo Biocompatibility and Improved Compression Strength of Reinforced Keratin/Hydroxyapatite Scaffold

Jie Fan,Meng-Yan Yu,Tong-da Lei,Yong-Heng Wang,Fu-Yuan Cao,Xiao Qin,Yong Liu 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.2

A rapid freezing/lyophilizing/reinforcing process is suggested to fabricate reinforced keratin/hydroxyapatite (HA) scaffold with improved mechanical property and biocompatibility for tissue engineering. The keratin, extracted from human hair, and HA mixture were rapidly frozen with liquid nitrogen and then lyophilized to prepare keratin/HA laminar scaffold. The scaffold was then immersed in PBS for reinforcement treatment, and followed by a second lyophilization to prepare the reinforced keratin/HA scaffold. The morphology, mechanical, chemical, crystal and thermal property of the keratin/HA scaffold were investigated by SEM, FTIR, XRD, DSC, respectively. The results showed that the keratin/HA scaffold had a high porosity of 76.17 ± 3%. The maximum compressive strength and compressive modulus of the reinforced scaffold is 0.778 and 3.3 MPa respectively. Subcutaneous implantation studies in mice showed that in vivo the scaffold was biocompatible since the foreign body reaction seen around the implanted scaffold samples was moderate and became minimal upon increasing implantation time. These results demonstrate that the keratin/HA reinforced scaffold prepared here is promising for biomedical utilization.

Epidemiological trend in inflammatory bowel disease in Taiwan from 2001 to 2015: a nationwide population-based study

Hsu-Heng Yen,Meng-Tzu Weng,Chien-Chih Tung,Yu-Ting Wang,Yuan Ting Chang,Chin-Hao Chang,Ming-Jium Shieh,Jau-Min Wong,Shu-Chen Wei 대한장연구학회 2019 Intestinal Research Vol.17 No.1

Background/Aims: Incidences of inflammatory bowel disease (IBD), ulcerative colitis (UC), and Crohn’s disease (CD), havebeen increasing in Asia. In this study, we report the relevant clinical characteristics and determined the epidemiological trend ofIBD in Taiwan from 2001 to 2015. Methods: A retrospective study was conducted to analyze data recorded from January 2001through December 2015 in the registered database compiled by the National Health Insurance and provided by the Ministry ofHealth and Welfare, Taiwan. Results: A total of 3,806 patients with catastrophic IBD illness were registered from 2001 to 2015 inTaiwan (CD, 919; UC, 2,887). The crude incidence of CD increased from 0.17/100,000 in 2001 to 0.47/100,000 in 2015, whereasthat of UC increased from 0.54/100,000 in 2001 to 0.95/100,000 in 2015. The prevalence of CD increased from 0.6/100,000 in2001 to 3.9/100,000 in 2015, whereas that of UC increased from 2.1/100,000 in 2001 to 12.8/100,000 in 2015. The male-to-femaleratio in the study sample was 2.19 for CD and 1.62 for UC. The median age of those registered with CD was lower than that ofthose registered for UC: 38.86 and 44.86 years, respectively. A significantly greater increase in CD incidence rate was identifiedamong 20 to 39-year-old compared with other age groups. Conclusions: Using Taiwan’s nationwide insurance database, we determinedthat the number of patients with CD increased more rapidly during the study period than the number of patients withUC, especially among age 20 to 39-year-old, resulting in a decreased UC-to-CD ratio. (Intest Res 2019;17:54-62)