Transcriptional activation of insulin-like growth factor binding protein 6 by 17β-estradiol in SaOS-2 cells

Yu-yan Zhao,Lei Guo,Xiao-juan Zhao,Hong Liu,Tian Lei,Dong-jie Ma,Xiao-yu Gao 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.7

Osteoblasts can synthesize the insulin-like growth factors (IGFs) and the IGF-binding proteins (IGFBPs), which may either enhance or attenuate IGF-stimulated bone cell proliferation. Since estrogen induced osteoblastic differentiation and proliferation through an estrogen- responsive gene in target cells, we investigated the effects of estrogen on IGFBP-6 expression in the human osteoblastic-like cell line SaOS-2. Expressions of IGFBP-6 protein and mRNA increased 2.8 and 2-fold, respectively, in the presence of 17-β-estradiol (E2) (0.01 to 1 μM) and estrogen receptor (ER) in SaOS-2 cells. On the other hand, E2 induced a 2-fold increase in SaOS-2 cell proliferation. To identify genomic sequences associated with estrogen responsiveness, the 5'-promoter region (-44 to +118) of the IGFBP-6 gene was cloned into a chloramphenicol acetyltransferase (CAT) reporter vector. E2 induced a 3-fold increase in CAT activity in SaOS-2 cells transiently transfected with this construct. Identification of the estrogen- responsive element (ERE) [5'-CCTTCA CCTG-3'] (-9 to +1) in this IGFBP-6 gene promoter region was confirmed using electromobility shift assays and deletion analysis. This functional ERE was important for E2-induced trans-activation of the IGFBP-6 gene. These results demonstrate that E2 exhibits a positive effect on IGFBP-6 gene transcription through estrogen- liganded ER binding to the functional ERE in the IGFBP-6 gene promoter in SaOS-2 cells. Osteoblasts can synthesize the insulin-like growth factors (IGFs) and the IGF-binding proteins (IGFBPs), which may either enhance or attenuate IGF-stimulated bone cell proliferation. Since estrogen induced osteoblastic differentiation and proliferation through an estrogen- responsive gene in target cells, we investigated the effects of estrogen on IGFBP-6 expression in the human osteoblastic-like cell line SaOS-2. Expressions of IGFBP-6 protein and mRNA increased 2.8 and 2-fold, respectively, in the presence of 17-β-estradiol (E2) (0.01 to 1 μM) and estrogen receptor (ER) in SaOS-2 cells. On the other hand, E2 induced a 2-fold increase in SaOS-2 cell proliferation. To identify genomic sequences associated with estrogen responsiveness, the 5'-promoter region (-44 to +118) of the IGFBP-6 gene was cloned into a chloramphenicol acetyltransferase (CAT) reporter vector. E2 induced a 3-fold increase in CAT activity in SaOS-2 cells transiently transfected with this construct. Identification of the estrogen- responsive element (ERE) [5'-CCTTCA CCTG-3'] (-9 to +1) in this IGFBP-6 gene promoter region was confirmed using electromobility shift assays and deletion analysis. This functional ERE was important for E2-induced trans-activation of the IGFBP-6 gene. These results demonstrate that E2 exhibits a positive effect on IGFBP-6 gene transcription through estrogen- liganded ER binding to the functional ERE in the IGFBP-6 gene promoter in SaOS-2 cells.

Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

RBF Neural Networks-Based Robust Adaptive Tracking Control for Switched Uncertain Nonlinear Systems

Lei Yu,Shumin Fei,Xun Li 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.2

In this paper, a robust adaptive H∞ control scheme is presented for a class of switched uncertain nonlinear systems. Radical basis function neural networks (RBF NNs) are employed to approximate unknown nonlinear functions and uncertain terms. A robust H∞ controller is designed to enhance robustness due to the existence of the compound disturbance which consists of approximation errors of the neural networks and external disturbance. Adaptive neural updated laws and switching signals are deducted from multiple Lyapunov function approach. It is proved that with the proposed control scheme, the resulting closed-loop switched system is robustly stable and uniformly ultimately bounded (UUB) such that good capabilities of tracking performance is attained and H∞ tracking error performance index is achieved. A practical example shows the effectiveness of the proposed control scheme.

Improved Work Function of Preferentially Oriented Indium Oxide Films Induced by the Plasma Exposure Technique

Lei Yang,Shuai Guo,Qiuling Yang,Yuankun Zhu,Bing Dai,Hailing Yu,Pei Lei,Jiecai Han,Ying Hou,Jiaqi Zhu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.6

The preferentially oriented In2O3 thin films were prepared on glass substrates by conventional magnetron sputtering with Ar+ plasma exposure at room temperature. Based on the x-ray diffraction, x-ray photoelectron spectroscopy, and UV photoelectron spectroscopy results, it was found that the Ar+ plasma exposure not only enhanced the low-temperature crystallization of In2O3 thin films, but also led to a dramatic improvement in the work function. Furthermore, it demonstrated that the shift mechanism of the work function in In2O3 thin films mainly combined with theelimination of oxygen defects and the change of the preferential orientation of In2O3 film surface.

Influences of Three Oidiodendron maius Isolates and Two Inorganic Nitrogen Sources on the Growth of Rhododendron kanehirae

Lei-Chen Lin,Wan-Rou Lin,Yu-Chen Hsu,Hung-Yu Pan 한국원예학회 2020 원예과학기술지 Vol.38 No.5

Rhododendron kanehirae Wilson is an endemic species of Taiwan that is listed as extinct in the wild by the Red List of Vascular Plants of Taiwan. R. kanehirae seedlings can form mycorrhizae with the ericoid mycobiont Oidiodendron maius. A completely randomized experimental design was conducted to investigate the effects of three different O. maius isolates (strains CBS110450, RhYM3, and RooDK1) as well as nitrate and ammonium (inorganic nitrogen) on the growth of R. kanehirae by evaluating height and shoot, root, and total fresh weight. Three isolates of O. maius showed different colony morphology and growth rate, which was further verified by molecular analysis. The results showed that application of ammonium fertilizer improved the height and shoot, root, and total fresh weight of all of the R. kanehirae seedlings. Among them, seedlings inoculated with RhYM3 had the highest fresh weights and heights. By applying an appropriate amount of nitrogen combined with RhYM3 inoculation, the rhizome of R. kanehirae grew well. The information provided here is crucial for the conservation of R. kanehirae, as this species is on the verge of extinction.

An Improved ORB Algorithm of Extracting and Matching Features

Lei Yu,Zhixn Yu,Yan Gong 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.5

Synthesis and densification of ultra-fine ZrC powders-effects of C/Zr ratio

Yu, Lei,Feng, Lun,Lee, Hyung Ik,Silvestroni, Laura,Sciti, Diletta,Woo, Yi Jin,Lee, Sea-Hoon Elsevier 2019 International journal of refractory metals & hard Vol.81 No.-

<P><B>Abstract</B></P> <P>The excess carbon and oxygen contents of ZrC nano-powders was controlled, and the resultant effects on the densification of ZrC was analyzed. The particle size of the synthesized ZrC powder was about 200 nm and its oxygen content was 0.49 wt%. The good results can be attributed to the rapid heating and cooling rate, the beneficial effects of current, and the relatively low synthesis temperature by using SPS (Spark plasma sintering) for the powder synthesis. The homogeneous distribution between reactants was an important factor to minimize the formation of excess carbon. With increasing the amount of carbon in the raw powder mixture, the oxygen content of ZrC powders decreased and the densification was suppressed. The decrease of excess carbon content, the presence of oxygen in the ZrC lattice, and the fine particle size promoted the sintering of ZrC ceramics without any additives at relatively low temperature and pressure (1750 °C, 40 MPa).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ultra-fine ZrC powders were synthesized using a spark plasma sintering apparatus. </LI> <LI> ZrC powder with particle size of 200 nm and oxygen content of 0. 49 wt% was obtained. </LI> <LI> The powder can be densified without additives at low temperature and pressure. </LI> <LI> Low carbon content, and the oxygen in the lattice were benefit for the densification. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Balancing between "Two Evils"

Yu Lei,Sui Sophia 한국학술연구원 2022 Korea Observer Vol.53 No.2

This paper finds that Pyongyang's nuclear issue is by no means China's "primary contradiction (top priority)" in its international relations. Chinese priority on the Korean peninsula is to keep stability and the status quo of a divided Korea rather than rush to find a resolution to the nuclear issue, which may result in a military imbalance on the Peninsula, or the collapse of the Pyongyang regime and hurt China's interest. To this end, China has adopted the strategy of making a delicate balance between pressuring and sustaining the Pyongyang regime. The paper concludes that from Chinese perspectives, a North Korea at the nuclear threshold contributes to counterbalancing US military presence in the region, maintaining a status quo of divided Koreas and helping China overcome US containment. China aspires to achieve three interconnected objectives with this strategy. This first is to sustain China's economic growth, the second to counterattack US containment, and the third to propel China's rise at the systemic (global) level.

Pretreatment Thrombocytosis as a Prognostic Factor in Women with Gynecologic Malignancies: a Meta-analysis

Yu, Min,Liu, Lei,Zhang, Bing-Lan,Chen, Qi,Ma, Xue-Lei,Wu, Yu-Ke,Liang, Chun-Shui,Niu, Zhi-Min,Qin, Xin,Niu, Ting Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background: This study was performed to analyze the prognostic implications of pretreatment or preoperative thrombocytosis in women with gynecologic malignancies. Material and Methods: We surveyed 2 medical databases, PubMed and EMBASE, to identified all relevant studies. A total of 14 (n=3,490) that evaluated the link between thrombocytosis and 5-year survival were included. REVMAN version 5.1 was used for our analysis and publication bias was evaluated using the Begg's funnel plot and tested by STATA 11.0. Risk ratios (RRs) with 95% confidence intervals (CIs) generated by the random effect model were used to assess the strength of any association. Results: 709(20.3%) of the 3,490 patients exhibited thrombocytosis (platelet counts > $400{\times}10^9/L$) at primary diagnosis, and their mortality was 1.62-fold higher compared with the others (RR=1.62, 95%CI=[1.28-2.05], p<0.0001). Thrombocytosis failed to have a stronger effect on the survival of advanced patients of stages III to IV in our study (n=478, RR=1.29, 95% CI=[1.13-1.48], p=0.0003), nor in women with cervical cancer in stage IB (n=1371, RR=1.73, 95% CI=[1.71-2.58], p=0.007). In addition, when adjusted for different carcinoma, it was associated with worse prognosis for all except the ones with vulvar cancer (n=201, RR=0.43, 95% CI=[0.14-1.29], p=0.13). Conclusions: This meta-analysis indicated that thrombocytosis might be associated with a worse prognosis for patients with gynecologic malignancies but without specificity or sensitivity for the ones in advanced stage. When adjusted for different gynecologic malignancies, it showed a significant effect on survival of all except vulvar cancers.

Synthesis of Porous Carbon Supported Palladium Nanoparticle Catalysts by Atomic Layer Deposition: Application for Rechargeable Lithium–O₂ Battery

Lei, Yu,Lu, Jun,Luo, Xiangyi,Wu, Tianpin,Du, Peng,Zhang, Xiaoyi,Ren, Yang,Wen, Jianguo,Miller, Dean J.,Miller, Jeffrey T.,Sun, Yang-Kook,Elam, Jeffrey W.,Amine, Khalil American Chemical Society 2013 Nano letters Vol.13 No.9

<P>In this study, atomic layer deposition (ALD) was used to deposit nanostructured palladium on porous carbon as the cathode material for Li–O<SUB>2</SUB> cells. Scanning transmission electron microscopy showed discrete crystalline nanoparticles decorating the surface of the porous carbon support, where the size could be controlled in the range of 2–8 nm and depended on the number of Pd ALD cycles performed. X-ray absorption spectroscopy at the Pd K-edge revealed that the carbon supported Pd existed in a mixed phase of metallic palladium and palladium oxide. The conformality of ALD allowed us to uniformly disperse the Pd catalyst onto the carbon support while preserving the initial porous structure. As a result, the charging and discharging performance of the oxygen cathode in a Li–O<SUB>2</SUB> cell was improved. Our results suggest that ALD is a promising technique for tailoring the surface composition and structure of nanoporous supports in energy storage devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2013/nalefd.2013.13.issue-9/nl401833p/production/images/medium/nl-2013-01833p_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl401833p'>ACS Electronic Supporting Info</A></P>