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Increased 11β-hydroxysteroid dehydrogenase type 1 contributes to barrier dysfunction in aged skin
( Beom Jun Kim ),( Noo Ri Lee ),( Chung Hyeok Lee ),( Young Bin Lee ),( Solam Lee ),( Hyun Jee Hwang ),( Eunjung Kim ),( Eung Ho Choi ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2
Background: 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) converts inactive cortisone into cortisol, an active form, and is expressed by several tissues including the skin. Excessive active glucocorticoid (GC) deteriorates skin barrier function. Objectives: To find out if 11β-HSD1 affects on the barrier function in aged skin. Methods: We have performed human and in vivo studies. We measured cortisol in stratum corneum (SC) and oral epithelium of the elderly and young. Hairless mice were used for 11β-HSD1 immunohistochemistry staining of skin and measuring skin barrier function and serum cytokines. 11β-HSD1 knock-out (KO) mice and its wild-type were used for measuring lipid synthesis related enzyme. Results: Cortisol levels were elevated in SC and oral epithelium of the elderly rather than young. The 11β -HSD1 expression was increased in immunohistochemistry stain of aged mice skin. Aged mice showed decreased transepidermal water loss (TEWL) and SC hydration, and increased SC integrity than young. Serum inflammatory cytokines including interleukin-1α, -4, -10, -31 and tumor necrosis factor-α were increased in aged mice than young. The expressions of lipid synthesis related enzymes in epidermis were elevated in KO mice and topical 11β -HSD1 inhibitor applied mice. Conclusion: 11β-HSD1 expression is elevated in aged skin. It increases active GC and then deteriorates skin barrier function.
도시노인의 신체적 여가활동참여와 사회심리적 건강과의 관계
최영옥,구봉진,임나리 한국체육대학교 체육과학연구소 2000 韓國體育大學校附屬 體育科學硏究所論文集 Vol.19 No.1
This paper is to clarify the relationship between participation in physical leisure activities, past exercise experience and social psychological health in the urban aged. For this purpose, through multi-stage random sampling, a total of 328 (176 males; 152 females) were selected among the old in their 65 to 75 in Seoul. As for the measuring instrument, SAS (Self-rating Anxiety Scale), developed by Zung(1971), and the questionnaires, translated by Jang Dong-san et al. (1988), were used for the measurement of anxiety; SDS (Self-rating Depression Scale), developed by Zung and Durham (1965) and translated by Jang Dong-san (1988), was used for the measurement of melancholy; the questionnaire, translated and standardized by Baek He-won (1993) from SES (Self-Esteem scale) of Rosenberg (1965), was used for the measurement of self-esteem; and LSI-W Life Satisfaction Index-Well-Being), standardized by Bigot(1974), was used for life satisfaction. Through analysis of the relationship between participation in physical leisure activities, past exercise experience and social psychological health in the urban aged, the conclusion was drawn as follows: There are differences in social psychological health according to physical leisure activities. Both males and females showed statistically significant differences in self-esteem, life satisfaction, melancholy and anxiety according to participation in physical leisure activities. That is, as compared with the group of non-participation in exercises, the groups of regular participation and participation in exercises related with sports showed higher self-esteem and life satisfaction, and lower melancholy and anxiety
이연리,김보경,최원호,김태경,이창권,배영민,조성일 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-
Smooth muscle contraction is regulated by intracellular Ca^(2+) ([Ca^(2+)]_(i)) and the phosphorylation of myosin light chain (MLC). However, various kinds of vasoconstrictors induce a further contraction at a given [Ca^(2+)]_(i), and elicit a sustained contraction under Ca^(2+) -depleted conditions, referred to as "Ca^(2+) -sensitization", in intact and membrane-pen-neabilized smooth muscle. Previously, several molecules, including protein kinase C (PKC), RhoA, and mitogen-activated protein kinases (MAPK), have been suggested as candidate regulators of Ca^(2+) -sensitization. In the present review, we describe the role of PKC, RhoA, and MAPK in the regulation of Ca^(2+) -sensitization, and suggest a new model in research for the regulation of smooth muscle contraction.
한약재 주정 추출물이 종자발아와 유묘생장에 미치는 영향
김진효 ( Jin Hyo Kim ),김준영 ( Jun Young Kim ),류성지 ( Sung Ji Ryu ),최근형 ( Geun Hyoung Choi ),김원일 ( Won Il Kim ),김세리 ( Se Ri Kim ),박병준 ( Byung Jun Park ),조남준 ( Nam Jun Cho ) 한국환경농학회 2014 한국환경농학회지 Vol.33 No.3
BACKGROUND: Herbal extracts have been screened fortheir inhibitory effect of seed germination and rootdevelopment on weeds, but there is a scarcity of reports forcrop growth regulation. The objective of this research wasto develop a growth inhibitor on Brassica campestris, andits effective extraction method from herbal medicineextract. METHODS AND RESULTS: Eighty four herbal medicineextracts were tested for their plant growth inhibitionactivity on B. campestris. The alcohol extracts of Artemisiaannua, Cinnamomum cassia, and Mentha arvensisinhibited over 30% of germination and the extract of A. annua, and C. cassia inhibited over 70% of radicle growthat 0.1 % w/w treatment. The partially purified extracts of A. annua, and C. cassia with dichloromethane and hexaneshowed stronger radicle growth inhibition than the crudeextracts on B. campestris. The diethyl ether extract of A. annua showed a similar 50% radicle growth inhibition (RI50= 45 mg/L) to its partially purified extract withdichloromethane or hexane, but the diethyl ether extract ofC. cassia showed a worse RI50 than the purified extract. CONCLUSION: The alcohol extracts of A. annua, and C. cassia showed potent radicle growth inhibition propertieson B. campestris. Diethyl ether proved to be a good solventfor simple extraction from A. annua.
Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes
Choi, Ran,Kim, Bo-Hwan,Naowaboot, Jarinyaporn,Lee, Mi-Young,Hyun, Mi-Ri,Cho, Eun-Ju,Lee, Eun-Soo,Lee, Eun-Young,Yang, Young-Chul,Chung, Choon-Hee Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.12
Diabetic nephropathy is the most serious complication in diabetes mellitus. It is known that oxidative stress and inflammation play a central role in the development of diabetic nephropathy. In this study, we investigated that ferulic acid (FA) known as anti-oxidative agent could effect on diabetic nephropathy by anti-oxidative and anti-inflammatory mechanism. We examined the effects of FA in obese diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats and non-diabetic control Long-Evans Tokushima Otsuka (LETO) rats. We treated FA to experimental rats from 26 to 45 weeks of age. We evaluated ACR, MDA and MCP-1 in 24 h urine and examined renal histopathology and morphologic change in extracted kidneys from rats. Also, we evaluated the ROS production and MCP-1 levels in cultured podocyte after FA treatment. In the FA-treated OLETF rats, blood glucose was significantly decreased and serum adiponectin levels were increased. Urinary ACR was significantly reduced in FA-treated OLETF rats compared with diabetic OLETF rats. In renal histopathology, FA-treated OLETF rats showed decreased glomerular basement membrane thickness, glomerular volume, and mesangial matrix expansion. FA treatment decreased oxidative stress markers and MCP-1 levels in 24 h urine of rats and supernatants of cultured podocyte. In conclusion, it was suggested that FA have protective and therapeutic effects on diabetic nephropathy by reducing oxidative stress and inflammation.
Choi, Yu-Ri,Jung, Sung-Chul,Shin, Jinhee,Yoo, So Young,Lee, Ji-Su,Joo, Jaesoon,Lee, Jinho,Hong, Young Bin,Choi, Byung-Ok Korean Society of Medical Genetics and Genomics 2015 대한의학유전학회지 Vol.12 No.1
Purpose: Charcot-Marie-Tooth disease (CMT) is a peripheral neuropathy mainly divided into CMT type 1 (CMT1) and CMT2 according to the phenotype and genotype. Although molecular pathologies for each genetic causative have not been revealed in CMT2, the correlation between cell death and accumulation of misfolded proteins in the endoplasmic reticulum (ER) of Schwann cells is well documented in CMT1. Establishment of in vitro models of ER stress-mediated Schwann cell death might be useful in developing drug-screening systems for the treatment of CMT1. Materials and Methods: To develop high-throughput screening (HTS) systems for CMT1, we generated cell models using transient expression of mutant proteins and chemical induction. Results: Overexpression of wild type and mutant peripheral myelin protein 22 (PMP22) induced ER stress. Similar results were obtained from mutant myelin protein zero (MPZ) proteins. Protein localization revealed that expressed mutant PMP22 and MPZ proteins accumulated in the ER of Schwann cells. Overexpression of wild type and L16P mutant PMP22 also reduced cell viability, implying protein accumulation-mediated ER stress causes cell death. To develop more stable screening systems, we mimicked the ER stress-mediated cell death in Schwann cells using ER stress inducing chemicals. Thapsigargin treatment caused cell death via ER stress in a dose dependent manner, which was measured by expression of ER stress markers. Conclusion: We have developed genetically and chemically induced ER stress models using Schwann cells. Application of these models to HTS systems might facilitate the elucidation of molecular pathology and development of therapeutic options for CMT1.
Choi, Ji-Young,Kim, Ye Jin,Cho, Su-Jung,Kwon, Eun-Young,Ryu, Ri,Choi, Myung-Sook MDPI AG 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.4
<P>Taeeumjowuitang (TJ) is an alternative herbal medicine that has been used to treat obesity in Korea. The molecular mechanisms involved in TJ-induced anti-obesity effects have not yet been determined. The aim of the current study was to elucidate the effects of TJ on obesity and metabolic syndrome, by analyzing the transcriptional and metabolic responses to TJ treatment. C57BL/6J mice were fed a high-fat or high-fat + 3% (<I>w</I>/<I>w</I>) TJ diet for 12 weeks. Their phenotypic characteristics were measured and the anti-obesity mechanism was elucidated, based on the RNA sequencing (RNA-seq) transcriptomic profiles in an animal model of obesity. TJ treatment ameliorated insulin resistance, dyslipidemia, and hepatic steatosis in high-fat diet-induced obese mice, with a simultaneous reduction in body weight gain by enhancing energy expenditure and suppressing adiposity. An analysis of the global transcriptional changes by RNA-seq revealed that TJ upregulated mitochondrial oxidative phosphorylation-associated genes in epididymal white adipose tissue (eWAT), suggesting an enhanced mitochondrial function after TJ treatment. Moreover, TJ effectively attenuated the high-fat diet-induced inflammatory response through transcriptional changes in eWAT. Our findings provide some mechanistic insights into the effects of TJ, an alternative oriental medicine, in the treatment of obesity and its comorbidities. They demonstrate that metabolic and transcriptional responses to diet-induced obesity with TJ treatment were desirable in adipose tissue metabolism.</P>
Choi, Sik-Won,Moon, Seong-Hee,Yang, Hye Jeong,Kwon, Dae Young,Son, Young-Jin,Yu, Ri,Kim, Young Su,Kim, So I.,Chae, Eun Jeong,Park, Sang-Joon,Kim, Seong Hwan Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Antlers have been traditionally used for thousands of years as a natural product with medicinal and pharmaceutical properties. In developing healthy foods, <I>Bacillus</I>-mediated fermentation is widely used to enhance the biological activity of nutrients in foods. Recently, fermentation was shown to enhance the osteogenic activity of antlers. This study aimed to elucidate the antiresorptive activity of <I>Bacillus</I>-fermented antler and its mode of action. We found that <I>Bacillus</I>-fermented antler extract strongly inhibited osteoclast differentiation by downregulating the expression and activity of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1). This extract also inhibited the activation of phospholipase C<I><I>γ</I></I>2 (PLC<I><I>γ</I></I>2), a signaling molecule that could regulate NFATc1 transcriptional activity. This suggested that <I>Bacillus</I>-fermented antler extract could inhibit PLC<I><I>γ</I></I>2-NFATc1 signaling required for bone resorption and cell fusion. Consequently, <I>Bacillus</I>-fermented antler extract might benefit osteoclast-related disorders, including osteoporosis; furthermore, it may improve gastrointestinal activity.</P>