Selenium 공급방법이 수경재배 토마토의 생장과 Se 흡수에 미치는 영향

이철규(Cheol-Kyu Lee),조경철(Kyung-Cheol Cho),이정현(Jeong-Hyun Lee),조자용(Ja-Yong Cho),서범석(Beom-Seok Seo),양원모(Won-Mo Yang) (사)한국생물환경조절학회 2005 생물환경조절학회지 Vol.14 No.4

토마토 ‘모모타로’를 공시하여 셀레늄 10㎎ㆍL?¹의 관주 및 엽면시비 등의 단용 및 혼용처리가 수경재배 토마토의 생육 및 과실 내 셀레늄 축적 함량에 미치는 영향을 구명하였다. 토마토 종자를 50공 트레이에 파종하여 70일 동안 육묘한 후 유묘를 코코피트 슬라이브를 이용한 수경재배 시스템에 정식하였다. 양액은 일본원예시험장 배양액 표준처방으로 조성하였으며, pH 5.8~6.2와 EC 2.3 ㎎ㆍL?¹ 등으로 조절하여 공급하였다. 셀레늄은 무기태 SeO₂와 sugar fatty acid ester에 킬레이트화 한 유기태 셀레늄을 10 ㎎ㆍL?¹으로 조성하여 관주, 엽면시비, 관주와 엽면시비를 병행하여 처리하였다. 초장, 엽수, 엽면적 및 엽록소 등의 토마토 생장반응은 셀레늄의 엽면시비, 그리고 엽면시비와 관주를 병행한 처리구에서 현저히 증가하였다. 과실 내 셀레늄 축적 함량은 킬레이트화 한 유기태 셀레늄을 엽면시비와 관주를 병행하여 처리한 경우에 0.302 ㎎ㆍL?¹으로 가장 높았다. 무기태와 유기태 셀레늄의 엽면시비와 관주 등의 단용 처리 보다는 혼용 처리가 전반적인 과실 생장과 체내 셀레늄 축적에 효과적이었다. 무기태 설레늄(SeO₂) 보다는 sugar fatty acid ester에 킬레이트화한 유기태 셀레늄 처리가 셀레늄을 함유한 기능성 토마토의 수경재배에 더 효과적이었다. This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC 2.3 dSㆍm?¹ and pH 5.8~6.2 recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic SeO₂ (here in after referred to Se) and organic selenium chelated with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application of Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

우황청심원의 고문헌기록 및 실험적 연구결과 분석을 통한 임상응용 확대의 필요성 고찰

오영택,오현묵,김서우,김원용,손창규,조정효,Oh, Young-Taeck,Oh, Hyeon-Muk,Kim, Seo-Woo,Kim, Won-Yong,Son, Chang-Gue,Cho, Jung-Hyo 대전대학교 한의학연구소 2017 혜화의학회지 Vol.26 No.1

Objectives: The aim of this study is (1) to investigate the historic changes and pharmacological efficacies of Woohwangchungsim-won and (2) to discuss the necessities for Woohwangchungsim-won's clinical applications. Methods: This study was performed through (1) investigating the ancient literature records related with Woohwangchungsim-won and analyzing Woohwangchungsim-won's composition, dosage and indications, (2) searching articles about Woohwangchungsim-won on 10 major Korean web and 3 major foreign web article search engines and analyzing Woohwangchungsim-won's pharmacological efficacies and indications. Results: Woohwangchungsim-wom has been used for cerebrovascular diseases such as stroke and palpitation. Also, there are some ancient literature records of Woohwangchungsim-won's clinical applications in neuropsychiatric disorders such as depression and bipolar disorder. In addition, there have been a number of experimental studies which demonstrate Woohwangchungsim-won's neuroprotective effect on cerebral cortex and hippocampus injury. So, it is possible to infer that Woohwangchungsim-won can be used for the treatments of neuropsychiatric disorders associated with neuronal cell death in cerebral cortex and hippocampus. But there have been no or less experimental studies which demonstrate the pharmacological efficacy of Woohwangchungsim-won on such disease. Conclusion: It is necessary that further experimental studies which demonstrate Woohwangchungsim-won's pharmacological efficacy on neuropsychiatric disorders should be done and Woohwangchungsim-won's clinical applications should be expanded on the basis of those related experimental results.

Butein, a tetrahydroxychalcone, suppresses pro-inflammatory responses in HaCaT keratinocytes

( Won Yong Seo ),( Gi Soo Youn ),( Soo Young Choi ),( Jinseu Park ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.9

Up-regulation of cell adhesion molecules and proinflammatory cytokines contributes to enhanced monocyte adhesiveness and infiltration into the skin, during the pathogenesis of various inflammatory skin diseases, including atopic dermatitis. In this study, we examined the anti-inflammatory effects of butein, a tetrahydroxychalcone, and its action mechanisms using TNF--stimulated keratinocytes. Butein significantly inhibited TNF--induced ICAM-I expression and monocyte adhesion in human keratinocyte cell line HaCaT. Butein also decreased TNF-- induced pro-inflammatory mediators, such as IL-6, IP-10 and MCP-1, in HaCaT cells. Butein decreased TNF--induced ROS generation in a dose-dependent manner in HaCaT cells. In addition, treatment of HaCaT cells with butein suppressed TNF--induced MAPK activation. Furthermore, butein suppressed TNF--induced NF-kappaB activation. Overall, our results indicate that butein has immunomodulatory activities by inhibiting expression of proinflammatory mediators in keratinocytes. Therefore, butein may be used as a therapeutic agent for the treatment of inflammatory skin diseases. [BMB Reports 2015; 48(9): 495-500]

Intelligent Emotional Interface for Personal Robot and Its Application to a Humanoid Robot, AMIET

Yong-Ho Seo,Il-Woong Jeong,Hye-Won Jung,Hyun S. Yang 제어로봇시스템학회 2004 제어로봇시스템학회 국제학술대회 논문집 Vol.2004 No.8

[P6-04] Comparison of Fermentation Characteristics in Raw and Frozen Mulberry Wine

Won Seok Seo,Yun Jeong Hwang,Jae Jun Jeong,Yong-Taek Kim,Jae Young Cha,Yong Soo Lee 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Various Types of Pt-Ni Binary Catalysts Supported on the Carbon Nanotubes as Cathode Catalysts for DMFC

Seo, Yon Ki,Kim, Yong Hwan,Chung, Uoo Chang,Chung, Won Sub Trans Tech Publications, Ltd. 2007 Diffusion and defect data. SSP. [Pt. B], Solid sta Vol.119 No.-

<P>Several methods for loading of Pt-Ni binary catalysts on the CNTs were tested. Using the CNTs treated HNO3, the catalysts supported Pt, Ni separately(Pt&Ni/CNT) and Pt-Ni alloy(Pt- Ni/CNTs) were made respectively. The results of supporting were confirmed by TEM and XRD analysis. TEM images show that metal catalysts were loaded uniformly and finely on the CNTs. By XRD analysis, the peaks of Pt&Ni/CNTs displayed Pt and Ni peaks respectively. Also it was found that the peaks of Pt were shifted to the higher 2theta angle in X-ray diffraction pattern which indicated that Pt-Ni alloy was formed successfully(Pt-Ni/CNTs). In addition, electrochemical tests for cathode catalysts of DMFC were performed. As the results, Pt-Ni/CNTs was most suitable for cathode catalysts of DMFC.</P>

Clinical relevance of necrotizing change in patients with community‐acquired pneumonia

Seo, Hyewon,Cha, Seung‐,Ick,Shin, Kyung‐,Min,Lim, Jae‐,Kwang,Yoo, Seung‐,Soo,Lee, Jaehee,Lee, Shin‐,Yup,Kim, Chang‐,Ho,Park, Jae‐,Yong,Lee, Won‐,Kee BLACKWELL SCIENCE 2017 RESPIROLOGY Vol.22 No.3

<P><B>ABSTRACT</B></P><P><B>Background and objective</B></P><P>Few studies have analysed a large number of patients with necrotizing pneumonia (NP) diagnosed based on computed tomography (CT) scans. The aim of the present study was to document the incidence and clinical features of NP in patients with community‐acquired pneumonia (CAP).</P><P><B>Methods</B></P><P>This retrospective study was conducted on CAP patients who had been admitted to a tertiary referral centre and who had available enhanced CT scan images. Patients were allocated into NP and non‐NP groups, and they were compared with respect to various clinical variables.</P><P><B>Results</B></P><P>Of the 830 patients included in the present study, necrotizing change was observed in 103 patients (12%). Patients with NP experienced more symptoms of pneumonia, had higher blood levels of inflammatory markers and more often required pleural drainage compared to patients with non‐NP. Although the use of mechanical ventilation, vasopressor infusion, 30‐day mortality, in‐hospital mortality and clinical deterioration did not differ between the NP and non‐NP groups, the median length of hospital stay (LOS) was significantly longer in the NP group. Multivariate analysis using Cox proportional hazards model showed that necrotizing change independently predicted LOS in patients with CAP.</P><P><B>Conclusion</B></P><P>NP affects approximately one‐tenth of hospitalized CAP patients. It may be associated with more severe clinical manifestations and may increase the need for pleural drainage. NP was found to be an independent predictor of LOS, but not of mortality in CAP patients.</P>

Proteomic studies of putative molecular signatures for biological effects by Korean Red Ginseng

Yong Yook Lee,Hwi Won Seo,Jong-Su Kyung,Sun Hee Hyun,Byung Cheol Han,Songhee Park,Seung-Ho So,Seung Ho Lee,Eugene C. Yi 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: Korean Red Ginseng (KRG) has been widely used as an herbal medicine to normalize andstrengthen body functions. Although many researchers have focused on the biological effects of KRG,more studies on the action mechanism of red ginseng are still needed. Previously, we investigated theproteomic changes of the rat spleen while searching for molecular signatures and the action mechanismof KRG. The proteomic analysis revealed that differentially expressed proteins (DEPs) were involved inthe increased immune response and phagocytosis. The aim of this study was to evaluate the biologicalactivities of KRG, especially the immune-enhancing response of KRG. Methods: Rats were divided into 4 groups: 0 (control group), 500, 1000, and 2000 mg/kg administrationof KRG powder for 6 weeks, respectively. Isobaric tags for relative and absolute quantitation was performedwith Q-Exactive LC-MS/MS to compare associated proteins between the groups. The putativeDEPs were identified by a current UniProt rat protein database search and by the Gene Ontologyannotations. Results: The DEPs appear to increase the innate and acquired immunity as well as immune cell movement. These results suggest that KRG can stimulate immune responses. This analysis refined our targetsof interest to include the potential functions of KRG. Furthermore, we validated the potential moleculartargets of the functions, representatively LCN2, CRAMP, and HLA-DQB1, by Western blotting. Conclusion: These results may provide molecular signature candidates to elucidate the mechanisms ofthe immune response by KRG. Here, we demonstrate a strategy of tissue proteomics for the discovery ofthe molecular function of KRG.

식도 중복 낭종으로 오인된 식도 평활근종 1예

Won Yong Seo,김광하,이호석 대한상부위장관ㆍ헬리코박터학회 2021 Korean Journal of Helicobacter Upper Gastrointesti Vol.21 No.2

Esophageal leiomyoma is a benign tumor that accounts for two-thirds of esophageal benign tumors. In general, patients with esophageal leiomyoma are asymptomatic; however, as the tumor grows in size, symptoms such as dysphagia, chest discomfort, obstruction, or bleeding can appear. A 65-year-old man visited our clinic to receive treatment for a subepithelial tumor in the lower esophagus, which was incidentally found during endoscopy for work-up of chest discomfort and indigestion. On endoscopic ultrasonography (EUS), the tumor was 2.6×1.9 cm in size, with a cystic lesion located in the submucosal and proper muscle layers and thick cystic wall. Therefore, it was highly likely to be a duplication cyst. As the patient had symptoms such as chest discomfort, surgical resection was performed. The tumor was finally diagnosed as a leiomyoma with cystic degeneration. Leiomyoma with cystic change that has similar EUS features to duplication cyst is rare. Thus, we report the case with a literature review.

A hot-water extract of <i>Sanguisorba officinalis</i> ameliorates endotoxin-induced septic shock by inhibiting inflammasome activation : HSO attenuates inflammasome activation

Seo, Dong-Won,Cho, Yong-Il,Gu, Suna,Kim, Da-Hee,Yi, Young-Joo,Lee, Sang-Myeong Center for Academic Publications Japan 2018 Microbiology and immunology Vol.62 No.1

<P>The inflammasome is a multiprotein signaling complex that mediates inflammatory innate immune responses through caspase 1 activation and subsequent IL-1 secretion. However, because its aberrant activation often leads to inflammatory diseases, targeting the inflammasome holds promise for the treatment of inflammation-related diseases. In this study, it was found that a hot-water extract of Sanguisorba officinalis (HSO) suppresses inflammasome activation triggered by adenosine 5-triphosphate, nigericin, microbial pathogens, and double stranded DNA in bone marrow-derived macrophages. HSO was found to significantly suppress IL-1 production in a dose-dependent manner; this effect correlated well with small amounts of caspase 1 and little ASC pyroptosome formation in HSO-treated cells. The anti-inflammatory activity of HSO was further confirmed in a mouse model of endotoxin-induced septic shock. Oral administration of HSO reduced IL-1 titers in the serum and peritoneal cavity, increasing the survival rate. Taken together, our results suggest that HSO is an inhibits inflammasome activation through nucleotide-binding domain and leucine-rich repeat pyrin domain 3, nucleotide-binding domain and leucine-rich repeat caspase recruitment domain 4 and absent in melanoma 2 pathways, and may be useful for treatment of inflammasome-mediated diseases.</P>