복합화된 지방자치단체 청사의 범죄예방 설계 필요성 조사연구

김서우 한국셉테드학회 2022 한국셉테드학회지 Vol.13 No.3

This study investigates the perceptions of public officials and civil service users of mixed-use local government office buildings were investigated through a questionnaire survey, focusing on the following questions: are mixed-use government office buildings safe for public officials and service users? and are current government office building a satisfactory facility designed to adequately respond to various threats and damages resulting from an increase in malicious civil petitioners and protesters? This study confirms that social awareness related to rallies and demonstrations classified the types by satisfaction with crime prevention design compared to actual damage caused by civil petitioners and protesters. Ultimately, the purpose of this study is to evaluate and confirm the need to prepare for safety or security issues that may be triggered by the various functions of mixed-use government office buildings. The implications of this study are as follows: Additional facilities of government office building should be provided depending on the environment and the needs of the local community. However, it may be that the mixed-use building can act as a useful means to revitalize the local community and economy if functions and spaces that are suitable for each unique environment are properly provided.

Integrating omics analysis of salt stress-responsive genes in rice

김서우,정희정,정기홍 한국유전학회 2015 Genes & Genomics Vol.37 No.8

The detrimental effect of high salinity on crop production is a serious problem. However, the number of genes with known functions relating to salinity tolerance is very limited in rice. To effectively address this limitation, selection of useful candidate genes and identification of major regulatory factors through global approaches are necessary. To this end, we used three data series of affymetrix array data produced with salt-treated samples from NCBI Gene Expression Omnibus (http://www.ncbi.nlm. nih.gov/geo/) and identified 653 rice genes commonly differentially expressed under three salt-stress conditions. While evaluating the quality of selected candidate genes for salt-stress responses, Gene ontology enrichment analysis revealed that responses to salt and water stresses of biological process category are highly overrepresented in salt-stress conditions. In addition, the major salt stress-responsive metabolism process and regulatory gene modules are classified through MapMan analysis, and detailed elements for further studies are suggested. Based on this, we proposed a salt stress-responsive signaling pathway in rice. The functional analysis of the main signal transduction and transcription regulation factors identified in this pathway will shed light on a novel regulatory metabolism process that can be manipulated to develop crops with enhanced salinity tolerance.

TP-83 : Thematic Poster ; Associated Factors of Recurred Hemoptysis after Bronchial Artery Embolization in Patients with Active Pulmonary Tuberculosis or Post Tuberculosis Sequelae

김서우,이석정,류연주,이진화,장중현,최선영,강병철 대한결핵 및 호흡기학회 2013 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.116 No.-

Background: Hemoptysis is a serious complication that may present as a life-threatening emergency of tuberculosis (TB) patient. Bronchial artery embolization (BAE) has been well known as an effective management for immediate and recurrent hemoptysis. The aim of this study was to determine the risk factors contributing to rebleeding after BAE in patients with active or post tuberculosis sequelae. Methods: Following a retrospective review of clinical data of 253 patients who underwent BAE between 2006 and 2012, 167 patients were either a history of TB or current diagnosis of active TB enrolled. Follow up periods was 14.9 months. Results: The median age diagnosed of active TB was 38 years and the age experienced first hemoptysis was 53 years. Of 42 patients (25%) who recurred symptom, 13 patients showed rebleeding within 1 month, and 12 patients after 1year. The reactivation of TB, combined TB destroyed lung and consolidation in chest CT were risk factors of rebleeding after BAE in multivariate logistic regression analyses. The median non recurrence time was 9 months. The factors of significantly associated with the recurrence free time after BAE were TB destroyed lung (HR, 2.4; 95% CI, 1.3-4.5; p=0.005) and hypertrophied vessels in chest CT (HR, 3.6; 95% CI, 1.1-11.9; p=0.036) based on a Cox proportional hazard model. Conclusion: BAE was very effective treatment for the control of hemptysis in TB patients. In patient with severe TB sequelae, such as TB destroyed lung or hypertrophied vessels in chest CT, considered a high risk of rebleeding, should be required closed observation. Background: Hemoptysis is a serious complication that may present as a life-threatening emergency of tuberculosis (TB) patient. Bronchial artery embolization (BAE) has been well known as an effective management for immediate and recurrent hemoptysis. The aim of this study was to determine the risk factors contributing to rebleeding after BAE in patients with active or post tuberculosis sequelae. Methods: Following a retrospective review of clinical data of 253 patients who underwent BAE between 2006 and 2012, 167 patients were either a history of TB or current diagnosis of active TB enrolled. Follow up periods was 14.9 months. Results: The median age diagnosed of active TB was 38 years and the age experienced first hemoptysis was 53 years. Of 42 patients (25%) who recurred symptom, 13 patients showed rebleeding within 1 month, and 12 patients after 1year. The reactivation of TB, combined TB destroyed lung and consolidation in chest CT were risk factors of rebleeding after BAE in multivariate logistic regression analyses. The median non recurrence time was 9 months. The factors of significantly associated with the recurrence free time after BAE were TB destroyed lung (HR, 2.4; 95% CI, 1.3-4.5; p=0.005) and hypertrophied vessels in chest CT (HR, 3.6; 95% CI, 1.1-11.9; p=0.036) based on a Cox proportional hazard model. Conclusion: BAE was very effective treatment for the control of hemptysis in TB patients. In patient with severe TB sequelae, such as TB destroyed lung or hypertrophied vessels in chest CT, considered a high risk of rebleeding, should be required closed observation.

크론병 환자에서 azathioprine으로 유발된 췌장염

김서우,정성애,권경주,류민선,노선희,태정현,최주영,김성은,정혜경,심기남,김태헌,유권,문일환 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

결핵의 감별 진단을 위한 객담 검체에서 Real Time PCR의 임상적 유용성

김서우,장중현,심윤수,류연주,이진화 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.-

저가의 머신 비전 카메라를 이용한 2차원 진동의 측정 및 교정

김서우,이정권,Kim, Seo Woo,Ih, Jeong-Guon 한국음향학회 2018 韓國音響學會誌 Vol.37 No.2

현존하는 진동 측정 센서는 정밀도 면에서는 대부분의 진동을 측정하기에 충분하나, 센서 한 개로 하나의 지점이나 방향에 한정하여 측정할 수밖에 없다는 단점을 갖고 있다. 반면 카메라의 경우, 정밀도나 측정 가능한 주파수 영역의 면에서는 다소 불리하지만, 한 번에 광범위한 영역의 진동을 측정할 수 있고 가격 면에서 유리하며 다자유도의 진동을 동시에 측정할 수 있다는 큰 장점을 갖고 있다. 본 연구에서는 저가의 머신 비전 카메라가 진동 측정 센서로서 어느 정도의 오차 범위 내에서 진동을 측정할 수 있는지 알아본 후, 실제 외팔보의 진동을 측정하였다. 카메라의 2차원 평면 이미지는 두 방향의 직선 운동과 한 방향의 회전 운동을 나타낼 수 있다. 먼저 단일 점의 진동을 카메라로 측정하고, LDV(Laser Doppler Vibrometer) 측정을 기준으로 한 카메라 측정의 오차를 실험적으로 교정하였다. 다음으로 다중점의 진동을 한 번에 측정하여 회전 진동과 외팔보 전체 형상의 진동을 측정하였다. 외팔보 전체 형상 진동은 주파수와 시간 영역 모두에 대하여 분석하였다. The precision of the vibration-sensors, contact or non-contact types, is usually satisfactory for the practical measurement applications, but a sensor is confined to the measurement of a point or a direction. Although the precision and frequency span of the low-cost camera are inferior to these sensors, it has the merits in the cost and in the capability of simultaneous measurement of a large vibrating area. Furthermore, a camera can measure multi-degrees-of-freedom of a vibrating object simultaneously. In this study, the calibration method and the dynamic characteristics of the low-cost machine vision camera as a sensor are studied with a demonstrating example of the two-dimensional vibration of a cantilever beam. The planar image of the camera shot reveals two rectilinear and one rotational motion. The rectilinear vibration motion of a single point is first measured using a camera and the camera is experimentally calibrated by calculating error referencing the LDV (Laser Doppler Vibrometer) measurement. Then, by measuring the motion of multiple points at once, the rotational vibration motion and the whole vibration motion of the cantilever beam are measured. The whole vibration motion of the cantilever beam is analyzed both in time and frequency domain.

Metastatic Adrenocortical Carcinoma arising from an Adrenal Tumor in Cushing’s Syndrome

김서우,강준구,김철식,이성진,임성희 대한내과학회 2019 대한내과학회 추계학술대회 Vol.2019 No.1

흡인성 폐렴 환자의 병원 내 사망률과 그 예측 인자

김서우,류연주,홍성철,김경진,정가영,심윤수,이진화,장중현 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.0

Real-Time Polymerase Chain Reaction Assay: A Response to Recent Letter to the Editor

김서우,장정현 대한결핵및호흡기학회 2016 Tuberculosis and Respiratory Diseases Vol.79 No.1

Streptavidin이 융합된 DR4 항원에 특이적인 single-chain Fv 항체의 개발

김서우,우상욱,김진규,Kim, Seo Woo,Wu, Sangwook,Kim, Jin-Kyoo 한국미생물학회 2018 미생물학회지 Vol.54 No.4

Streptavidin (STR)과 Biotin system은 Biotin의 Streptavidin에 대한 높은 비공유 친화력(non-covalent affinity; $K_D=10^{-14}M$)과 4 Biotin 결합부위를 갖는 Streptavidin의 tetramer 구조로 인해 복수의 항원결합부위 및 복수의 항원특이성을 갖는 항체를 제조할 수 있기 때문에 가장 활발하게 연구되고 있다. 이 system을 활용하기 위해 우리는 Streptomyces avidinii 염색체 DNA로부터 PCR을 통해 Streptavidin (STR) 유전자를 증폭하고 이를 TRAIL (tumor necrosis factor ${\alpha}$ related apoptosis induced ligand) receptor인 death receptor 4 (DR4)에 특이적으로 결합하는 hAY4 single-chain Fv 항체유전자에 융합시켰다. 대장균에서 발현시킨 STR에 융합된 hAY4 ScFv (hAY4-STR) 항체는 가열시킨 SDS-PAGE에서 43 kDa monomer를 나타내었다. 그러나 가열하지 않은 SDS-PAGE와 Size-exclusion chromatography에서는 tetramer인 172 kDa을 나타내었는데 이는 hAY4 ScFv-STR 항체가 STR의 자연적인 비공유결합에 의해 유도된 tetramer를 형성하고 있음을 나타내고 있다. 본 융합 단백질은 Ouchterlony assay와 ELISA에서 보여주는 것처럼 자연 Streptavidin과 유사한 Biotin 결합력을 유지하고 있었다. ELISA와 Westernblot을 이용하여 정제된 hAY4-STR 융합항체의 DR4 항원결합력 또한 확인하였다. 게다가 표면 플라즈몬 공명(surface plasmon resonance) 분석에서 hAY4 ScFv-STR tetramer는 tetramerization에 의해 hAY4 ScFv monomer보다 60배 더 높은 항원결합력을 나타내었다. 요약하면 hAY4 ScFv-STR 융합단백질은 E. coli에서 soluble tetramer로 성공적으로 발현 및 정제되었으며 Biotin과 DR4 항원에 동시에 결합함을 보여 주었다. 이는 bifunctional and tetrameric ScFv 항체를 제조 할 수 있음을 제시해 주고 있다. The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.