황색종 연초건조에 있어서 건조조건에 따른 내용성분변화 제3보. 송풍량 변화에 따른 영향

석영선,황건중,손현주,Seok, Yeong-Seon,Hwang, Keon-Joong,Sohn, Hyun-Joo 한국연초학회 1986 한국연초학회지 Vol.8 No.2

This experiment was carried out to investigate the effect of circulating air volume to chemical composition of tobacco leaves during the bulk curing. The results were as follows : The decrease of circulating air volume prolonged curing time as the case of a 0.058m3/min. kg; but, no remarkable changes with a 0.11m3/min. kg and a 0.083m3/min. kg. When the circulating air volume was decreased, compared with 0.11m3/min. kg, sugar content was increased while total nitrogen and nicotine content showed a reduced values. In case of a 0.058m3/min. kg and a 0.083m3/min.kg, solanone and damascenone were high. Some of volatile neutral constituents and volatile organic acids were increased during curing process ; especially, their quantities were remarkably increased during the midrib drying stage. As circulating air volume decreased the equilibrium moisture content was increased, while leaf shatter index and filling capacity were decreased.

황색종 연초 건조에 있어서 건조조건에 따른 내용성분 변화 II. 중골건조기 승온속도에 따른 영향

석영선,황건중,이은홍,Seok, Yeong-Seon,Hwang, Keon-Joong,Lee, Eun-Hong 한국연초학회 1986 한국연초학회지 Vol.8 No.1

This experiment was carries out to study on the effect of temperature raising rate to chemical composition of tobacco leaves during the midrib drying stage of flue-curing. The results were as follows : In the case of the temperature raising rate became more rapidly during midrib drying stage. It had a tendency to increase in oxailic acid, succinlc acid, ammonia, polyphenol; there was a large loss of total sugar, reducing sugar, malic acid, palmitic acid and linolenic acid; leaf color became more reddish; the leaf quality index value decreased, thereby the quality of external appearance deteriorated. It is desirable that the temperature raising rate had to be more slowly.

생명공학에 의한 인(P) 자원의 절감과 재이용

김상범,석영선,Kim, Sang-Beam,Seok, Yeong-Seon 한국연초학회 2013 한국연초학회지 Vol.35 No.1

Effect of Phosphate Solubilizing Fungi on P Uptake and Growth of Tabacco in Rock Phosphate Applied Soil

박명수,석영선,정종배,안기섭,사동민,Park, Myung-Su,Singvilay, Olayvahn,Seok, Yeong-Seon,Chung, Jong-Bae,Ahn, Ki-Sup,Sa, Tong-Min Korean Society of Soil Science and Fertilizer 2003 한국토양비료학회지 Vol.36 No.4

The effect of phosphate solubilizing microbes (PSM) on plant P uptake and growth in rock phosphate applied soil was tested under a greenhouse condition. Tobacco plants were grown in nonsterilized soil inoculated with Penicillium oxalicum CBPS-3F-Tsa with or without rock phosphate application as P fertilizer. Phosphorus concentration in tobacco plants was increased by the application of rock phosphate, while inoculation of soil with fungi further significantly increased P concentration in tobacco plants compared with the noninoculated treatments. Phosphorus uptake by tobacco plants was also increased by the application of rock phosphate and PSM inoculation, and the significant comparison has been made with single rock phosphate treatment. Growth of tobacco plant was also significantly increased in the treatments receiving rock phosphate, while the combined application of rock phosphate and PSM further increased plant growth. It was concluded that the positive effect of PSM inoculation on plant growth was closely related in plant P content and uptake. These results suggest that Penicillium oxalicum CBPS-3F-Tsa could solubilize insoluble soil phosphates and rock phosphate which can promote growth and P uptake of tobacco plants. 인광석을 처리한 토양에서 인산 가용화균 Penicillium oxalicum CBPS-3F-Tsa이 식물의 인산 흡수와 생육에 미치는 효과를 온실조건에서 조사하였다. 인광석을 처리한 토양에서 담배 식물체중의 인산 농도는 증가하였으며, 인산 가용화균을 접종한 경우에는 인산 농도가 더욱 증가하였다. 식물체의 총 인산 흡수량도 인산 가용화균을 접종한 경우 증가하였다. 담배 식물의 생육 또한 인광석과 인산 가용화균을 동시에 처리한 경우 인광석만 처리한 경우에 비하여 통계적으로 유의성 있게 증가하였는데, 이러한 인산 가용화균의 생육 촉진 효과는 인산 흡수에 미치는 영향과 밀접한 관계를 보였다. 따라서 인산 가용화균과 인광석 처리에서 나타난 식물의 인산 흡수량 및 생육 증대 효과는 결국 접종한 인산 가용화균에 의한 난용성 인광석의 가용화에 따른 결과로 판단된다.

황색종 연초 건조중 탈수경과 및 풍속차가 건조엽의 특성에 미치는 영향

유명현,석영선,이운철,Ryu, Myong-Hyun,Seok, Yeong-Seon,Lee, Un-Chul 한국연초학회 1983 한국연초학회지 Vol.5 No.2

The effect of leaf dehydration process and air flow capacity of bulk curing on physical properties and composition of cured leaves was studied, respectively, during flue- curing. Cured leaves from excessive moisture during yellowing stage and those from rapid dehydration Process inevitably during later stages, tend towards lower equilibrium moisture contents, higher shatter index, hither protein nitrogen, and leaf scalding or deterioration of Beaves with redish cast. Early dehydration at the yellowing stage re suited in increasing of p Bamitic, stearic, linoleic, and linolenic acid contents, but showed reduction of brightness difference between upper and lower surface of the cured leaves, Leaf surface lipid decreased with the progress of curing stages, more conspicuously during later stage. Lowering air flow capacity of fan by 50oye during stem drying stage resulted in increasing of leaf surface lipid and 25oye decreasing of electric power consumption , but curing period and kerosene consumption were not affected.

황색종 연초 건조중 황변기 온습도차가 잎담배 색상 및 화학성분에 미치는 영향

황건중,석영선,이한석,Hwang, Keon-Joong,Seok, Yeong-Seon,Lee, Han-Seok 한국연초학회 1985 한국연초학회지 Vol.7 No.2

cent was carried out to study on the effect of temperature and humidity to chemical tobacco leaves during the yellowing stage. The results were follows : In the condition of high humidity and low temperature, yellowing time was delayed ; leaf color appeared lack clearness. In the higher temperature and the lower humidity during the yellowing stage : total sugar, reducing sugar and malic acid content were increased. Decomposition of nitrogenous components elevated in $38^{\circ}C$, 85%RH. Changes of total nitrogen content correlated with total curing time. Adecrease of linolenic acid with a corresponding increase of chlorogenic acid proceeded in the condition of low temperature and high humidity. In a view of tobacco quality by chemical components, the low temperature and high humidity during the yellowing stage decreased quality of tobacco leaves. It is considered to control of the proper condition of temperature and humidity during the yellowing.

담배 속 식물의 다양한 활용방안 모색

엄유리,이문순,이이,석영선,Um, Yu-Rry,Lee, Moon-Soon,Lee, Yi,Seok, Yeong-Seon 한국연초학회 2011 한국연초학회지 Vol.33 No.1

Genus Nicotiana has 76 species including N. tabacum. These plants are used not only as a material for cigarette manufacturing but also as ornamental plant, medicinal plant, poisonous substance plant, and bug repellent plant. N. tabacum is used as a main material for cigarette manufacturing with N. rustica. N. sylvestris and N. alata is used as ornamental plants because of their beautiful flowers and N. rustica is used for bug repellent or pesticide because of its high concentration of nicotine. N. glauca, a tree tobacco, is used for bio-fuel production. N. tabacum is used as a popular model plant system for degeneration, regeneration, and transformation. N. benthamiana is also used as a model system for foreign gene expression by agroinfiltration. The transformation ability of tobacco plant is a good target for molecular farming. Hepatitis B virus envelop protein, E. coli heat-labile enterotoxin, diabetes autoantigen, and cholera toxin B subunit were produced using tobacco plants. Secondary metabolites of tobacco include nicotine, anabasine, nornicotine, anatabine, cembranoid, solanesol, linoleic acid, rutin, lignin and sistosterol, and they are used for various medicine productions which cannot be produced by organic synthesis for their complicated structures. In conclusion, we have to understand the applicability of tobacco plant in detail and study to enlarge the usage of the plants.

아황산가스가 견조 과정중 잎담배 내용성분에 미치는 영향

황건중(Keon Joong Hwang),석영선(Yeong Seon Seok) 한국환경보건학회 1985 한국환경보건학회지 Vol.11 No.1

This experiment was carried out to study 00 the effect of S02 gas to chemical com_ position of tobacco leaves during flue -curing. The results were as follows : S02 gas io briquet was the major factor to damage with tobacco leaves. The damage ooly occured in a presence of moisture io tobacco leaves, it did oot occured after color fixing stage which is a li ttle leaf moisture. The daoger of damage to tobacco leaves lies in 10 ppm of S02 gas concentration. Follow with the S02 gas concentration increased, sugar and nitrogen ∞ntents became higher, po1yphenol contents were 105s, and the quality of tobacco leaves declin.

발효부산물 오니의 시용이 열무 생장과 토양화학성에 미치는 영향

홍순달(Soon Dal Hong),석영선(Yeong Seon Seok),사동민(Tong Min Sa) 한국환경농학회 2001 한국환경농학회지 Vol.20 No.1

To investigate the effects of waste sludge from antibiotic fermentation on the growth of young radish and chemical properties of soil, five levels of fertilizer, control (recommended fertilizer, N-P₂O_5-K₂O=160-59-104 ㎏/㏊), AS(control + sludge 1,000 ㎏/㏊), AC(control + conventional compost 1,000 ㎏/㏊), SNS(control - subtracting 30% N of sludge + sludge 1,000 ㎏/㏊) and SNC(control - subtracting 30% N of conventional compost + conventional compost 1,000 ㎏/㏊) were applied and radish was grown twice with same treatments on May and August in 1998. Germination rate and early growth of young radish grown with AS and SNS were lower than those grown in control and with AC, SNC. This negative effects by adding the sludge in the early growth seemed to be caused by damage of ammonia gas released during degradation of the sludge in soil. However, yield of young radish showed no significant difference among all the treatments including the AS and SNS at the 1st and 2nd experiment, and these suggested that the latter half of growth of young radish was accelerated by adding the sludge. Contents of T-N in young radish and inorganic N in soil showed a tendency to increase by adding the sludge while antibiotic substance, cephalosporin-C, was not detected in plant material and soils after harvest of young radish in both experiments. Consequently, waste sludge from antibiotic fermentation, which contains high levels of organic matter and nitrogen could be used as an useful resource in agriculture.

Expansin 유전자를 이용한 담배의 형질전환

최동수,김호방,김정회,신주식,석영선,정찬문,이이,Choi Dongsu,Kim Ho-Bang,Kim Jeong-Hoe,Shin Ju-Sik,Seok Yeong-Seon,Chung Chan-Moon,Lee Yi 한국연초학회 2005 한국연초학회지 Vol.27 No.2

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.