본태성 고혈압에서 염산에포니디핀 (NZ-105, 핀테20정�)의 유효성 및 안전성 : 제3상 다기관 임상시험 The phaseⅢ multicenter open trial

배종화,손일석,강흥선,조정휘,강재헌,김성원,이선영,양윤준,김영식,선우성 대한간질학회 2003 대한간질학회지 Vol.9 No.1

톨라신 농도에 따른 세포독성 기작과 Ni2+과 Zn2+ 에 의한 저해

윤영배 · 한지혜 · 김영기 忠北大學校 農業科學硏究所 2018 農業科學硏究 Vol.34 No.2

본문 참고

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

일부 초등학생의 비만도에 따른 칼슘과 철의 섭취상태와 혈청 농도에 관한 연구

이화성,김은영,배윤정,승정자 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

The purpose of this study was to evaluate the nutrition status of calcium and iron in 116 elementary school students with different obesity index. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum calcium and iron levels were evaluated based on 24-recall dietary recalls, anthropometric measurements and blood analysis. The mean height, weight, obesity index and blood pressure of total subjects were 150.4 cm, 45.5 kg, 5.8% and 118.2/76.8 mmHg. The mean % of body fat, SBP and WHR were higher while LBM and TBW were lower in over weight subject than those of other group. The iron intake of overweight was lower than that of underweight and normal subjects(p<0.05) and average calcium intakes were only 50% and 60% respectively of the RDA for Korean. Serum calcium and iron levels of a three groups were within the normal range. There were no significant differences in serum calcium and iron concentrations among three groups, however those of underweight group were a slightly lower than those of other two groups. There were significantly negative correlations between iron of intake and weight, obesity index, systolic pressure and body fat percent. Systolic blood pressure was negatively correlated to the intakes of energy, carbohydrate and protein. Energy intake was also negatively correlated to serum iron. To summarize the results, calcium and iron intakes should be supplied to elementary school students, especially those who were overweight. These indicated that overweight group had improper mineral nutrition status. Therefore, nutrition education and profound studies from many different focus for overweight groups are highly required.

Characterization of Phage-Resistant Strains Derived from Pseudomonas tolaasii 6264, which Causes Brown Blotch Disease

( Yeong-bae Yun ),( Ji-hye Han ),( Young-kee Kim ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12

Pseudomonas tolaasii 6264 is a representative strain that causes bacterial blotch disease on the cultivated oyster mushroom, Pleurotus ostreatus. Bacteriophages are able to sterilize the pathogenic P. tolaasii strains, and therefore, they can be applied in creating disease-free mushroom cultivation farms, through a method known as “phage therapy”. For successful phage therapy, the characterization of phage-resistant strains is necessary, since they are frequently induced from the original pathogenic bacteria in the presence of phages. When 10 different phages were incubated with P. tolaasii 6264, their corresponding phage-resistant strains were obtained. In this study, changes in pathogenic, genetic, and biochemical characteristics as well as the acquired phage resistance of these strains were investigated. In the phylogenetic analyses, all phage-resistant strains were identical to the original parent strain based on the sequence comparison of 16S rRNA genes. When various phage-resistant strains were examined by three different methods, pitting test, white line test, and hemolytic activity, they were divided into three groups: strains showing all positive results in three tests, two positive in the first two tests, and all negative. Nevertheless, all phage-resistant strains showed that their pathogenic activities were reduced or completely lost.

Changes in growth and active components of Angelica gigas Nakai in different cultivation environments

Yeong-Bae Yun,Dae-Hui Jeong,Hae-Yun Kwon,Yurry Um 한국응용생명화학회 2024 Journal of Applied Biological Chemistry (J. Appl. Vol.67 No.-

Angelica gigas Nakai belongs to the Unbelliferae family and was a representative medicinal plant with antiinflammatory, antioxidant and immunoenhancing activities. Recently, changes in the atmospheric environment, such as temperature, CO2 concentration and solar radiation, have continuously resulted in the reduction of cultivation area and production amount of A. gigas. They could affect the synthesis of secondary metabolites including nodakenin, decursin and decursinol angelate. This study aimed to investigate the atmospheric factors and the growth and content of active compounds of A. gigas. Air temperature and soil temperature were lowest in Jeongseon (northern region). Solar radiation was highest in Bonghwa (central region). Relative humidity was the lowest in Jeongseon from January to June, and it was the lowest in Bonghwa after July. At the Jeongseon experimental site, most of the growth characteristics of A. gigas were poor. The growth characteristics of A. gigas in Chungju and Bonghwa did not show any significant difference. The contents of active compounds were significantly highest in A. gigas collected from Chungju, but were significantly low in Jeongseon. Relative humidity showed a significant positive correlation with the content of the active compounds except for nodakenin. Decursin and decursinol angelate were significantly negatively correlated with solar radiation and soil moisture content, respectively. Most of growth characteristics of A. gigas, except for root length, showed a significant positive correlation with nodakenin content. The results of this study can be used as data for predicting the location of A. gigas cultivation according to the growth and active compounds of A. gigas in different cultivation regions.

Biological Characterization of Various Strains of Pseudomonas tolaasii that Causes Brown Blotch Disease

Yun, Yeong-Bae,Park, Seong-Wan,Cha, Jae-Soon,Kim, Young-Kee The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1

Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin, tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, $P1{\alpha}$ (6 strains), $P1{\beta}$ (16 strains), and $P1{\gamma}$ (1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the $P1{\alpha}$ and $P1{\gamma}$ strains, but not in the $P1{\beta}$ strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.

Biological Characterization of Various Strains of Pseudomonas tolaasii that Causes Brown Blotch Disease

Yeong-Bae Yun,Seong-Wan Park,차재순,김영기 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1

Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin,tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, P1α (6 strains), P1β (16 strains), and P1γ(1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the P1α and P1γ strains, but not in the P1β strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.

톨라신 저해 물질을 이용한 갈반병의 억제

윤영배 ( Yeong-bae Yun ),김민희 ( Min-hee Kim ),한지혜 ( Ji-hye Han ),김영기 ( Young-kee Kim ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.2

톨라신은 1.9 kDa의 펩티드 독소로서 Pseudomonas tolaasii에 의해 생성되며, 재배중 느타리버섯에 갈반병을 일으킨다. 톨라신은 막에 pore를 형성하여 세포 구조를 파괴하고, 버섯 재배의 생산성을 심하게 감소시킨다. 톨라신에 의한 세포독성의 작용 기작은 완전히 밝혀지지 않았지만, 분자다중화에 의해 세포막에 채널구조 형성으로 이루어진다. 그러므로, 톨라신과 작용하는 식품첨가물 중에 톨라신의 다중화결합을 통한 세포막 pore형성을 저해하는 물질이 있을 것이다. 본 연구에서는, 다양한 물질들이 톨라신의 활성을 저해함을 확인하고, 이들을 톨라신저해물질(TIF)이라 명명하였다. 대부분의 톨라신 저해물질들은 식품가공과정에 쓰이는 유화제였다. 다양한 종류의 저해물질 중에 지방산과 에스터 결합한 polyglycerol과 지방산과 에스터 결합한 sucrose 화합물이 10^-4-10^-5 M 농도범위에서 톨라신의 세포독성을 효과적으로 저해하였다. 이러한 저해물질들은 균상재배하는 느타리버섯에서 갈반병의 발생을 성공적으로 억제하였다. Tolaasin, a 1.9 kDa peptide toxin, is produced by Pseudomonas tolaasii and causes the brown blotch disease of cultivated oyster mushroom. It forms pores on the membrane and thus destroys cellular membrane structure, seriously reducing the productivity of mushroom cultivation. The mechanism of tolaasin-induced cytotoxicity is not known in detail. However, it has been reported to form a pore structure in the cytoplasmic membrane through the molecular multimerization. Therefore, food additives which can interact with tolaasin molecules may inhibit the pore formation by hydrophobic interactions with tolaasin molecules. In this study, various food additive materials have been identified as inhibitors of the tolaasin activity and named tolaasin-inhibitory factors (TIF). Most of TIFs are emulsifying agents for food processing procedures. Among various TIFs, polyglycerol and sucrose esters of fatty acids blocked effectively the cytotoxicity of tolaasins at the concentrations 10^-4-10^-5 M. These TIFs also successfully suppressed the blotch disease development in the shelf cultivation of oyster mushroom.

Zn²⁺와 Ni²⁺에 의한 톨라신 용혈활성 저해효과의 pH 의존성

윤영배 ( Yeong-bae Yun ),최태근 ( Tae-keun Choi ),김영기 ( Young-kee Kim ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3

Pseudomonas tolaasii에 의해 분비되는 톨라신은 펩티드 독소로서, 버섯 자실체 구조와 세포를 파괴하여 갈반병을 일으킨다. 톨라신의 독성은 용혈활성을 측정함으로서 평가하며, 이는 톨라신 분자가 적혈구 막에 pore를 형성하여 세포 구조를 파괴하기 때문이다. 이전 연구에서, Zn²⁺ 뿐만 아니라 Ni²⁺이 톨라신의 세포독성에 억제효과를 가짐을 확인하였다. Ni²⁺은 농도가 증가함에 따라 톨라신에 의한 용혈작용을 저해하였으며, 이의 Ki 값은 1.8 mM이었다. 용혈활성은 10 mM 이상의 농도에서 완전히 저해되었다. Ni²⁺의 효과는 pH에 따라 크게 변하지 않았으나, Zn²⁺의 톨라신 세포독성 억제 효과는 염기성 pH에서 크게 증가하였다. 완충액의 pH를 7에서 9로 증가시키면, 50% 용혈작용이 일어나는 시간인 T50은 1 mM Ni²⁺에 의해 조금 증가하였으나 100 μM Zn²⁺에서는 크게 증가하였다. Zn²⁺와 Ni²⁺을 반응용액에 동시에 처리하였을 때, 두 양이온의 상승효과는 모든 pH에서 나타났다. 서로 다른 pH 의존성을 보이는 두 금속이온의 분자적 설명은 톨라신의 pore 형성과 세포 독성에 관한 기작의 이해에 기여할 것이다. Tolaasin secreted by Pseudomonas tolaasii is a peptide toxin and causes brown blotch disease on the cultivated mushrooms by collapsing cellular and fruiting body structure. Toxicity of tolaasin was evaluated by measuring hemolytic activity because tolaasin molecules form membrane pores on the red blood cells and destroy cell membrane structure. In the previous studies, we found that tolaasin cytotoxicity was suppressed by Zn²⁺ and Ni²⁺. Ni²⁺ inhibited the tolaasin-induced hemolysis in a dose-dependent manner and its Ki value was 1.8 mM. The hemolytic activity was completely inhibited at the concentration higher than 10 mM. The inhibitory effect of Zn²⁺ on tolaasininduced hemolysis was increased in alkaline pH, while that of Ni2+was not much dependent on pH. When the pH of buffer solution was increased from pH 7 to pH 9, the time for 50% hemolysis (T50) was increased greatly by 100 μM Zn²⁺; however, it was slightly increased by 1 mM Ni²⁺ at all pH values. When the synergistic effect of Zn²⁺ and Ni²⁺ on tolaasin-induced hemolysis was measured, it was not dependent on the pH of buffer solution. Molecular elucidation of the difference in pH-dependence of these two metal ions may contribute to understand the mechanism of tolaasin pore formation and cytotoxicity.