High efficient rice transformation method by modifying co-cultivation conditions

Hyang-Mi Park,Yul-Ho Kim,Tae-Young Hwang,Chang In Yang,Ji Ung Jeung,Young Chan Cho,Kyoung Ho Kang,Chung-Kon Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5㎎/ℓ L-cysteine, 1mM sodium thiosulfate, 1mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5㎎/ℓ silver nitrate). Co-cultivation temperature (23. 5℃ for 1 day, 26.5℃ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

DSM-Ⅲ-R 성격장애 진단의 내용타당도

양병환,조연규,김창현,황순택,오동열 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

The purpose of this study was to examine the content validity of DSM-Ⅲ-R personality disorder(PDs). A 3-point rating scale of 150 preliminary diagnostic criteria including 95 DSM-Ⅲ-R PD diagnostic criteria was sent to psychiatrists. Each item in this scale was rated by psychiatrists(n=101) in term of prototypicality to every DSM-Ⅲ-R PD. The results show that each PD criteria set of DSM-Ⅲ-R was registered high level of interrater consistency and items listed in the DSM-Ⅲ-R were, for the most part, rated as very prototypical to their DSM-Ⅲ-R diagnosis. Some criteria were, however, not rated to characteristic features to their DSM-Ⅲ-R diagnosis. 11 items of DSM-Ⅲ-R diagnostic criteria scored high not only to their DSM-Ⅲ-R diagnosis but also other diagnosis. 16 items rated as high prototypicality item of other diagnosis. Another 16 items seemed not to be appropriate to any of DSM-Ⅲ-R PD diagnosis. These results suggest that the content validity of the DSM-Ⅲ-R personality disorder is not so high.

水稻의 登熟向上에 關한 硏究

諸商律,李寅杰,김랑춘,李準琸,張南日,洪鍾旭 慶北大學校 1974 論文集 Vol.18 No.-

This study was made to get the cultural information for increasing yield with recently improved variety, investigating the effects of different transplanting date and seed size, the number of plant per hill on the growth, and the yield and yield components in rice. The results obtained were summaried as follows: 1. In larger seed, the percentage of germination was higher and the early growth was more vigorous. However, there appeared no effects of seed size in the later growing period. 2. When the young plants sown on April 26 were transplanted on 5th, 15th, 25th of June and 5th of July with 10-days interval, the heading date was promoted in earlier transplanted plots. The days to heading, however, was shortend. 3. In the case of the earlier transplanting in Tongil variety, the maximum number of tillers was increased, the percentage of effective tillers was decreased, and the rate of ripened grains was slightly higher. The yield was the highest in the transplanted plot of June 25. The next came the transplanted plots of 25th, 5th of June and 5th of July. In the early transplanted plot of Palkweong variety, heading was also promoted. The days required for heading were also proportionly shortend, the number of the effective tillers was smaller and the percentage of ripened grains also decreased. The productivity was the highest in transplanted plot of June 25. 4. When the number of plants per hill was increased, the heading date was promoted, the number of panicles was high and the number of grains per panicles was decreased. In the early transplanting of Tongil variety, the yields was the highest in 4-plants plot. When transplanted late, it was the highest in 6-plant plot. In Palkweong variety, there was general tendency of increasing of yield as the number of plants per hill increased. 5. The dwarf disease of Tongil variety and the stripe disease of Palkweong variety occurred much in case of the lesser number of plants per hill and the earlier transplanting.

성격장애 진단준거 개발을 위한 전형성 분석

황순택,조연규,오동열,김창현,양병환 漢陽大學校 精神健康硏究 1994 精神健康硏究 Vol.13 No.-

성격장애 진단준거목록의 타당도 연구

황순택,정재홍,조연규,오동렬,김창현,양병환 漢陽大學校 精神健康硏究 1999 精神健康硏究 Vol.18 No.-

Changes of Sex Hormones and Cervical Mucus in Estrus-synchronized Himalayan Tahrs (Hemitragus jemlahicus) in Non-breeding Season

Yong, Hwan-Yul,Kim, Myoung-Ho,Ha, Yong-Hee,Kim, Han-Woong,Kim, Mi-Young,Cho, Dong-Gil,Oh, Chang-Sik,Kim, Doo-Hee,Lim, Yang-Mook,Yoo, Mi-Hyun,Kim, Seung-Dong 韓國受精卵移植學會 2011 한국동물생명공학회지 Vol.26 No.3

Eight female Himalayan tahrs (Hemitragus jemlahicus) were estrus-synchronized, and transcervically inseminated with frozen-thawed semen in September, 2009, about 2 to 3 months earlier than their natural breeding season. Intravaginal progesterone-releasing devices were inserted into vaginas of six Himalayan tahrs on September 7, and the other two on September 8 to suppress luteal function of ovaries. The devices had been placed deep inside the vagina prior to withdrawal on September 23. A day before CIDR removal, a combination of PMSG 400 IU and hCG 200 IU was intramuscularly injected. Forty hours later, frozen-thawed semen was transcervically inseminated. Pregnancy diagnosis was performed 39 days later by analyzing progesterone level of serum. Every treatment was done under anesthesia inducted by xylazine injection. In conclusion, vaginal discharge of cervical mucus, hormonal changes induced by implant-typed or muscularly injectable hormones and widening of cervix enough to insert an insemination gun into uterine body were achieved in non-breeding season. Moreover, the first inseminated Himalayan tahr, 36 hours after CIDR removal was assumed to be pregnant but the fetus may have been lost due to the use of anesthetic drug.

토지이용변화에 따른 한천유역의 홍수유출 변동 분석

양세창 ( Se Chang Yang ),양성기 ( Sung Kee Yang ),이준호 ( Jun Ho Lee ),정우열 ( Woo Yul Jung ),고광효 ( Kwang Hyo Ko ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.4

A number of projects for development have been done continuously due to the increase of tourist in Jeju Island. However flood disaster countermeasure due to urbanization is not considered during this development projects. This study is to make basic process for the flood estimation in Han stream of Jeju Island. The variation of stream discharge due the every 5 years` land use change from 1980 to 2005. Data for flood events (rainfall and discharge) were collected for HEC-HMS model. Clark method was used for unit hydrograph analysis. For the estimation of Clark unit hydrograph parameters, Kraven II and Sabol`s empirical equations were applied. The peak discharge increased 9.9~33.67% and total discharge amount increased 12.53~30.21%. Also, time of concentration for peak discharge was reduced by 10 minutes for each event.

Dispersal Ability of Aedes albopictus Estimated by Mark-Release-Recapture Experiment in Korea

Sung-Chan Yang,E-Hyun Shin,Kyu-Sik Chang,Jong Yul Roh,Mi-Yeoun Park,Wook-Gyo Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

We conducted a investigation for dispersal ability of Aedes albopictus, a dengue vector mosquito, using mark-release-recapture method in Korea. A certain number of fluorescence marked Ae. albopictus was released in four different habitats – urban (Cheonan), suburban (Jochiwon), port (Pyeongtaek), and airport (Cheongju), and sticky traps and BG-traps were used to recapture the mosquito in designated time period. In case of urban and suburban areas, 100 sticky traps were used in 3-week period for both regions, and 19 BG-traps and 15 BG-traps were used in 36-hour period for urban and suburban areas respectively. Using sticky trap, 21 from 1,300 released mosquitoes (1.62%) were recaptured in urban area; while in suburban area, only 2 individuals (0.1%) were recaptured. Most of them were recaptured within 100m from releasing point and about 40% were within 20m distance. When BG-traps were used, 35 from 900 released mosquitoes (3.8%) were recaptured in urban area; while 26 mosquitoes (2.8%) were recaptured in suburban area. The maximum distance of dispersal was 200m in urban and 100m in suburban area, while most of them were recaptured in 50 ~ 80m range. In case of port and airport area, 15 and 10 BG-traps were used respectively to recapture 1,000 released mosquitoes in 5-day period. In port area, 23 mosquitoes (2.3%) were recaptured; while 166 mosquitoes (16.6%) were recaptured in airport area. The maximum distance of dispersal was 130m in both areas; while most of them were recaptured in 50m range. In conclusion, Ae. albopictus has a narrow dispersal range compared to other mosquitoes, a typical characteristic of genus Aedes, and their dispersal ability is greatly affected by various environmental factors. These results can provide basic information for effective prevention of Ae. albopictus and overseas inflow of dengue fever.

Distribution of Mosquitoes Collected Using BG-sentinel Trap around Cheongju International Airport and Pyeongtaek Port, Korea

Sung-Chan Yang,E-Hyun Shin,Kyu-Sik Chang,Jong Yul Roh,Mi-Yeoun Park,Wook-Gyo Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

As a part of disease vector and possible overseas inflow caused by global warming monitoring around airport and port area, the distribution of domestic mosquitoes was investigated using BG-sentinel trap. Mosquitoes were collected during 5-days per each area in early September, using 10 traps in Cheongju (airport) and 15 traps in Pyeongtaek (port). The numbers of mosquitoes collected in Cheongju and Pyeongtaek area were 1,762 including 10 species and 1,042 including 11 species respectively. In Cheongju, Aedes albopictus (657 individuals, 37.3%) and Culex pipiens complex (415 individuals, 23.6%) were dominant species; while in Pyeongtaek, Cx. pipiens complex (806 individuals, 77.4%) was dominant followed by Anopheles spp. (90 individuals, 8.6%) and Ochlerotatus koreicus (79 individuals, 7.6%). Similar species were collected in both Choengju and Pyeongtaek area. Although most species collected in both Cheongju and Pyeongtaek area were the same, Cx. inatomii was only collected in Pyeongtaek. In case of the Genus Aedes, more number of Ae. albopictus was collected compared to Oc. koreicus in Cheongju; while Oc. koreicus was more in Pyeongtaek. In case of the genus Culex, the individuals belonging to the vishinui group were collected such as Cx. bitaeniorhynchus, Cx. tritaeniorhynchus and Cx. orientalis. This study may provide basic information for efficient prevention of vector mosquito and potential overseas inflow of diseases, also we would try to expand different area in Korea.

흰쥐에서 폐 방사선 조사 후 interleukin - 1α ( IL-1α ) 및 interleukin - 6 ( IL-6 ) 발현에 대한 연구

장광열(Kwang Yul Chang),손춘희(Choon Hee Son),양두경(Doo Kyung Yang),김기현(Ki Hyun Kim),이혁(Hyuk Lee),윤선민(Seon Min Youn),양광모(Kwang Mo Yang),허원주(Won Joo Hur),노미숙(Mee Sook Roh),정진숙(Jin Sook Jeong),한진영(Jin Yeong Han 대한내과학회 2002 대한내과학회지 Vol.62 No.4

연구배경: 방사선 조사는 흉부 종양의 치료에 중요한 역할을 하지만 방사선 폐렴 때문에 용량의 제한을 받는다. 이런 폐 손상의 기전을 이해함으로써 부작용을 줄이고 손상을 예방할 수 있는 방법을 찾기 위하여 폐 섬유화에 기인한다고 알려져 있는 interleukin-1 alpha (IL-6 α)와 interleukin-6 (IL-6)의 방사선 폐 손상에서의 역할을 알아보기 위하여 이 연구를 시작하였다. 실험 방법: 30마리의 흰쥐를 대상으로 우측 폐에 20 Gy를 단일 조사하고, 20주까지 2주 간격으로 흰쥐를 희생시킨 후 절제한 폐조직에 대하여 일반병리조직학적 검사 및 IL-1α와 IL-6에 대한 면역조직화학적 연구를 실시하여 결과를 분석하였다. 결과: 방사선 조사 1∼2주 후까지는 폐간질의 부종, 모세혈관의 울혈이 주된 소견이었다. 4주까지는 폐포내에 대식구 및 림프구 등의 단핵구와 단백양 물질이 증가하였고, 폐포간격에도 단핵구들의 침윤이 관찰되었으며 이러한 소견은 8주까지 진행되었다. 6주부터 20주까지 염증세포의 침윤과 더불어 점차 진행되는 폐섬유화를 관찰하였다. IL-1α 및 IL-6의 발현정도에 대한 염색은 전 기간을 통하여 증가하는 양상을 보였지만 3주 전후까지는 급격한 증가를 보였고 그 후부터는 감소하는 경향을 나타내었다. 염색반응은 주로 폐포간질, 폐포내 및 기관지주위의 대식구, 폐포세포, 혹은 혈관내피세포에서 관찰되었다. 12주에서 20주까지는 섬유모세포에서도 염색되었지만 섬유화가 진행된 병변에서는 그 염색정도가 약한 반면 주변 폐포부위의 염색정도는 강하게 나타났다. 방사선 조사로 유도한 흰쥐의 폐손상은 시간의 경과에 따라 방사선 폐렴에서 폐섬유화로 진행되었고 이러한 폐손상의 진행에 따른 IL-1α 및 IL-6의 발현에 대한 염색정도는 급격히 증가하여 3주경에서 최고치를 나타내다가 그 이후부터는 감소하였지만 조사 후 희생까지의 전기간에 걸쳐 증가되어 있었다. 결론: IL-1α 및 IL-6는 방사선 조사 후 섬유화성 진행을 보이기 전단계에서 발현되어 폐섬유화 유발인자로 작용하는 것으로 생각된다. Background: One of the limitation during the irradiation of malignant tumor is hazard to normal tissue although it is important and effective tool for treating malignant tumor. We studied the role of interleukin-1 alpha (IL-1α) and interleukin-6 (IL-6) in the radiation-induced lung injury especially on fibrosis. Methods: We irradiated right-side lungs of thirty Sprague-Dawley rats with single fraction of 20 Gy and then sacrificed the animals until 20th week at intervals of two weeks. Both irradiated and unirradiated lung tissues were stained hematoxilin and eosin, Masson trichrome, reticulin and immunohistochemical staining for IL-1α and IL-6. The degree of the staining for IL-1α and IL-6 were examined semiquantitatively. Results: Two weeks after irradiation interstitial edema and capillary congestion appeared, followed by increase of the monocytes infiltration and proteinaceous material during 4th and 8th week. After eight weeks of irradiation, collagen and reticulin fibers were detected along alveolar wall. 12th to 20th week, fibrosis in interstitium, decreased number of alveoli and thickening of bronchial wall were observed. The degree of immunohistochemical staining for IL-1α and IL-6 was increased rapidly during the first three week and then decreased slowly, but remain increased until 20th week. Conclusion: Our Study demonstrate the early and persistent elevation of cytokines IL-1α and IL-6 by immunohistochemical stain in rat lung following pulmonary irradiation. We think cytokines are produced immediately after irradiation, make collagen genes turn on and perisist until the expression of late effects become apparent pathologically and clinically. (Korean J Med 62:453-464, 2002)