LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2

Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.

KLF4 suppresses the tumor activity of cutaneous squamous cell carcinoma (SCC) cells via the regulation of SMAD signaling and SOX2 expression

Li, Xue Mei,Kim, Soo Jung,Hong, Dong-Kyun,Jung, Kyoung Eun,Choi, Chong Won,Seo, Young-Joon,Lee, Jeung-Hoon,Lee, Young,Kim, Chang-Deok Elsevier 2019 Biochemical and biophysical research communication Vol.516 No.4

<P><B>Abstract</B></P> <P>Kruppel-like factor 4 (KLF4) is a zinc-finger transcription factor that plays a role in terminal differentiation of epidermal keratinocytes. There are conflicting reports regarding the role of KLF4 in tumor development, with both the tumor suppressive and/or oncogenic properties depending on different conditions and cell types. In this study, we investigated the functional importance of KLF4 in cutaneous squamous cell carcinoma (SCC). Immunohistochemistry showed that KLF4 expression was relatively low in SCC lesion compared to normal epidermis. To examine the effects of KFL4, we transduced SCC lines (SCC12 and SCC13 cells) with the KLF4-expressing recombinant adenovirus. Overexpression of KLF4 significantly decreased cell proliferation and colony forming activity. In addition, overexpression of KLF4 markedly reduced invasive potential, along with the downregulation of epithelial-mesenchymal transition (EMT)-related molecules. In a mechanistic study, KLF4 inhibited SOX2, of which expression is critical for tumor initiation and growth of SCC. Further investigations indicated that SOX2 expression is induced by TGF-β/SMAD signaling, and that overexpression of KLF4 inhibited SMAD signaling via upregulation of SMAD7, an important inhibitory SMAD molecule. Based on these data, KLF4 plays a tumor suppressive role in cutaneous SCC cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Overexpression of KLF4 decreased the cell proliferation, colony forming activity, invasion and EMT potential in cutaneous SCC cells. </LI> <LI> Overexpression of KLF4 decreased SOX2 level via inhibition of SMAD2 phosphorylation. </LI> <LI> Overexpression of KLF4 inhibited SMAD signaling via upregualtion of SMAD7, an inhibitory SMAD molecule. </LI> </UL> </P>

Stimulation of keratinocyte differentiation by extract of combined medicinal plant

Xue Mei Li,Dae-Kyoung Choi,Seul Ki Lim,In Suk Ahn,Dong-Il Kim,Yong Jun Piao,Chang Deok Kim,Jeung-Hoon Lee 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.4

Skin is the outermost organ and acts as a barrier between the organism and environment. Skin protects the organism from environmental insults, such as chemicals, pathogens, and UV light. Much of the protective function of skin is dependent on the epidermis, a multi-layered epithelium that is composed of various cell types such as keratinocytes and melanocytes. Keratinocytes produce protective components through a sophisticated differentiation process. Disturbance of keratinocyte differentiation is related to several skin diseases such as psoriasis and atopic dermatitis. In this study, we prepared extract of combined medicinal plants (ECMP) consisting of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia, Glycyrrhiza uralensis Fischer, and root bark of Ulmus davidiana. We demonstrated that ECMP enhanced keratinocyte differentiation and barrier functionality using an in vitro cell culture system and in vivo animal test. Treatment of cultured keratinocytes with ECMP resulted in induction of keratinocyte differentiation, as evidenced by increased differentiation markers such as involucrin, loricrin, and filaggrin. In line with these results, ECMP decreased proliferation of keratinocytes cultured in vitro. ECMP applied topically to tape-stripped mouse skins accelerated reduction of transepidermal water loss (TEWL), indicating fast recovery of barrier function. Immunohistochemistry showed that ECMP increased the filaggrin level in tape-stripped mouse skins. These results suggest that ECMP may be applicable for keratinocyte differentiation-related skin diseases.

Sorphinol C, a New Free-Radical Seavenger from Sorangium cellulosum

( Xue Mei Li ),( Tae Kyung Yu ),( Jong Hwan Kwak ),( Byoung Yil Son ),( Young Wan Seo ),( Ok Pyo Zee ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.3

KLF4 suppresses the tumor activity of cutaneous squamous cell carcinoma cells via the regulation of SMAD signaling and SOX2 expression

( Xue Mei Li ),( Chang Hyeon Kim ),( Dong Kyun Hong ),( Kyoung Eun Jung ),( Chong Won Choi ),( Young Lee ),( Chang Deok Kim ),( Young-joon Seo ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Kruppel-like factor 4 (KLF4) is a zinc -finger transcription factor that plays a role in terminal differentiation of epidermal keratinocytes. There are conflicting reports regarding the role of KLF4 in tumor development, with both the tumor suppressive and/or oncogenic properties depending on different conditions and cell types. Objectives: In this study, we investigated the functional importance of KLF4 in cutaneous squamous cell carcinoma (SCC). Methods: To examine the effects of KFL4, we transduced SCC lines (SCC12 and SCC13 cells) with the KLF4- expressing recombinant adenovirus. Results: Immunohistochemistry showed that KLF4 expression was relatively low in SCC lesion compared to normal epidermis. Overexpression of KLF4 significantly decreased cell proliferation and colony forming activity. In addition, overexpression of KLF4 markedly reduced invasive potential, along with the down-regulation of epithelial-mesenchymal transition (EMT)-related molecules. In a mechanistic study, KLF4 inhibited SOX2, of which expression is critical for tumor initiation and growth of SCC. Further investigations indicated that SOX2 expression is induced by TGF-β /SMAD signaling, and that overexpression of KLF4 inhibited SMAD signaling via up-regulation of SMAD7, an important inhibitory SMAD molecule. Conclusion: Based on these data, KLF4 plays a tumor suppressive role in cutaneous SCC cells.

Effects of Fermented Sea Tangle on DSS Induced Colitis in C57BL/6J Mice

Xue Mei Li,Jiale Song,Kun-Young Park 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Synergistic Increase of Oxidative Stress and Tumor Markers in PAH-Exposed Workers

Gao, Mei-Li,Chen, Lei,Li, Yong-Fei,Xue, Xiao-Chang,Chen, Lan,Wang, Li-Na,Shah, Walayat,Kong, Yu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

In this study, we investigated oxidative stress and tumor marker levels of polycyclic aromatic hydrocarbons (PAHs) in 136 coke oven workers and in 60 control subjects, and evaluated the correlation between oxidative stress and tumor marker levels. Questionnaires on basic demographic information were also administered. Significant differences in employment time and percentages of alcohol drinkers were observed between the control and exposed groups. PAH exposure was assessed using urinary 1-hydroxy-pyrene (1-OHP) levels and was found to be significantly higher in workers than in the controls. Significant differences (P<0.001) of MDA, GST, LDH, NSE, Cyfra21-1, and of SCC and TNF-a (P<0.0001 and P<0.05, P<0.001, respectively) levels were observed among controls and coke-oven workers, except for bottom coke oven workers. Associations between age and risk of increased TNF-a, smoking and increased GST activities, and drinking with increased MDA concentrations, were marginal (P=0.055, P=0.048, P=0.057, respectively). The association between smoking with MDA (P=0.004), NSE (P=0.005), SCC (P=0.004) andTNF-a (P<0.001), and drinking with TNF-a levels was significant (P=0.012). In addition, a significant positive correlation between oxidative stress and tumor markers was found in the present study. These results suggest that a synergistic increase of oxidative stress and tumor markers induced by PAHs may play a role in toxic responses for PAHs in coke oven workers.

보문 : 중국 경극 가면을 응용한 패치워크 기법의 의상 디자인 연구

이설매 ( Xue Mei Li ),이상희 ( Sang Hee Lee ),한설아 ( Sul Ah Han ) 한국의류학회 2012 한국의류학회지 Vol.36 No.2

Historically, garments have been spontaneously made with human instinct in order to warm and decorate the body using textiles that have been made and used by various peoples in different cultures. Contemporary garments have been used as a means of warming as well as express individual personality and a desire to pursue beauty. They have a unique formativeness and a symbolic nature according to cultural phenomena that reflect a cultural outcome that expresses contemporary thinking and ideology as well as their aesthetic consciousness. This study globally promotes Chinese opera mask culture and proposes the possibility of creative thinking through the introduction of Chinese opera mask elements into contemporary fashion, expressing them as patchworks. In order to endow artistic value (required in contemporary fashion) this study creates works by analyzing and applying a formative sense seen in patchwork techniques and the characteristics of Chinese opera masks; in addition, it sought the possibility of new expressions in garment designs. The results obtained from the research involve the following. First, the introduction of the traditional patchwork technique to contemporary garments may be a dynamic theme of garment expression and exceed stereotypical ideas that enable the creation of designs attuned to the globalization era. Second, it was possible to pursue independent formativeness which is in harmony with a modern sense through the restructuring of the harmony of colors and practical characteristics found in patchworks. Third, it was possible to demonstrate their artistry and unique effect in the expression of contemporary garments by the expression of the analyzed results of characteristic patterns of Chinese opera masks with a patchwork technique in creating works. Results show that it is necessary to conduct research into a patchwork technique that applies diverse materials as a new method to develop contemporary garment design also as a creative design. In addition, if the analyzed results of the characteristic patterns of Chinese opera masks can be expressed with a patchwork technique, they may be able to exhibit a genuine effect along with the artistry of expression in contemporary garments; thereby, relevant follow-up research should be further continued.

문장부호 정보와 확장된 청크에 기반한 중국어 최장명사구 식별

백설매(Xue-Mei Bai),김미훈(Mei-Xun Jin),이금희(Jin-Ji Li),정유진(You-Jin Chung),이종혁(Jong-Hyeok Lee) 한국정보과학회 언어공학연구회 2005 한국정보과학회 언어공학연구회 학술발표 논문집 Vol.2005 No.10

명사구는 기본명사구와 최장명사구로 분류된다. 최장명사구에 대한 정확한 식별은 문장의 전체적인 구문구조를 파악하고 문장의 정확한 지배용언을 찾아내는데 중요한 역할을 수행한다. 본 논문에서는 확장된 청크(chunk) 개념과 다섯 개의 클래스로 세분화된 문장부호 정보를 사용한 최장명사구 식별 기법을 제안한다. 제안된 기법은 기본모델(baseline)보다 4.05% 향상된 평균 88.63%의 우수한 F-measure 성능을 보인다.

New Triterpenoids from the Fruits of Schisandra wilsoniana and Their Biological Activities

Gao, Xue-Mei,Li, Yun-Qi,Shu, Li-Dan,Shen, Yan-Qiong,Yang, Li-Ying,Yang, Liu-Meng,Zheng, Yong-Tang,Sun, Han-Dong,Xiao, Wei-Lie,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3

Investigation of an organic extract of the fruits Schisandra wilsoniana led to the isolation of two new highly oxygenated nortriterpenoids, named schilancidilactones V-W (1-2). Their structures were elucidated by spectroscopic evidence. Compounds 1-2 feature a double bond between C-7 and C-8 compared with related known nortriterpenoids isolated from the genus Schisandra. Compounds 1 and 2 were tested for their anti-HIV-1 activities and cytotoxicity. The results revealed that compounds 1 and 2 showed moderate anti-HIV-1 activities with $EC_{50}$ 3.05 and 2.87 ${\mu}g/mL$, respectively, and compound 1 showed high cytotoxicity against KB and MDA-MB-231 cell with $IC_{50}$ values of 3.18 and 5.22 ${\mu}M$, respectively.