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Automatic cystocele severity grading in transperineal ultrasound by random forest regression
Ni, Dong,Ji, Xing,Wu, Min,Wang, Wenlei,Deng, Xiaoshuang,Hu, Zhongyi,Wang, Tianfu,Shen, Dinggang,Cheng, Jie-Zhi,Wang, Huifang Elsevier 2017 Pattern recognition Vol.63 No.-
<P><B>Abstract</B></P> <P>Cystocele is a woman disease that bladder herniates into vagina. Women with cystocele may have problem in urinating and higher risk of bladder infection. The treatment of cystocele highly depends on the severity. The cystocele severity is usually evaluated with the manual transperineal ultrasound measurement for the maximal distance between the bladder and the lower tip of symphysis pubis in the Valsalva maneuver. To improve the efficiency of the measurement, we propose a fully automatic scheme that can measure the distance between the two anatomic structures in each ultrasound image. The whole measurement scheme is realized with a two-phase random forest regression to infer the locations of the two structures in the images for the support of distance measurement. The experimental results suggest automatic distance measurements and the final grading by our random forest regression method are comparable to the measurements and grading scores from three medical doctors, and thus corroborate the efficacy of our method.</P> <P><B>Highlights</B></P> <P> <UL> <LI> First automatically computerized cystocele grading method on the transperineal ultrasound is developed in this study. </LI> <LI> The automatic cystocele grading on the transperineal ultrasound is realized with a two-phase random forest regression model. </LI> <LI> Auto-context features are helpful for our regression model to improve the cystocele grading results. </LI> </UL> </P>
Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis
Qi Xiao,Yuhong Hu,Xiaohong Yang,Jianna Tang,Xiaoshuang Wang,Xiaomin Xue,Mengxue Li,Minjing Wang,Yinan Zhao,Jingze Liu,Hui Wang 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.2
The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.
Proteome Changes in Penicillium expansum Grown in a Medium Derived from Host Plant
( Xiaoshuang Xia ),( Huan Li ),( Fei Liu ),( Ye Zhang ),( Qi Zhang ),( Yun Wang ),( Peiwu Li ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
Penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a twodimensional gel electrophoresis-based proteomic approach was applied to probe changes in P. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing of P. expansum. A total of 28 protein spots that were up-regulated in P. expansum when grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses of P. expansum to the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.
Xie Tao,Zhang Wei,Chen Chengjie,Wang Xiaoshuang,Zhang Jing,Luan Aiping,He Yehua 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6
Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifi cally expressed in the early stage of SE in pineapple ( Ananas comosus ), suggesting that the promoter of SERK1 might contain specifi c cis acting element regulating SE. To identify embryonic cell-specifi c element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was signifi cantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specifi c. Besides, a promoter lacking − 943 to -922 was shown to signifi cantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confi rmed again the 5’ upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our fi ndings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.
Influence of Solution Concentrations on Surface Morphology and Wettability of ZnO Thin Films
Jianguo Lv,Changlong Liu,Feng Wang,Zhitao Zhou,Zhenfa Zi,Yuan Feng,Xiaoshuang Chen,Feng Liu,Gang He,Shiwei Shi,Xueping Song,Zhaoqi Sun 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.2
ZnO thin films were grown on silicon substrates using a hydrothermal method. The XRD patterns show that all of the peaks can be attributed to the wurtzite structures of ZnO. The TC value of (002) plane and average crystal size increase first and then decrease with the increase of solution concentration. SEM and AFM results show that many dense hexagonal cylinder particles have been observed on the surface of the thin films, which grown at 0.08 and 0.10 mol/L. The surface roughness of the thin films deposited at 0.06,0.08, 0.10, and 0.12 mol/L are 24.5, 38.3, 32.0, and 39.4 nm, respectively. Surface wettability results show that the preferential orientation along c-axis and surface roughness contribute significantly to the hydrophobicity. The reversible switching between hydrophobicity and hydrophilicity is related to the synergy of the transition of wetting model, surface crystal structure, and surface roughness.
Ming YongFei,Li Yin,Chu JianZhi,Zhou XiaoShuang,Huang YuXuan,Yang ShuDe,Mu YueJun,Wang Lin,Zhang Rui,Cheng XianHao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-
Taiwanofungus camphoratus has attracted much attention because it can abundantly produce various active substances that exhibit blood-sugar lowering, immunity improving, and antioxidant properties. Currently, T. camphoratus is cultured using four main methods: cutting wood culture, solid-state fermentation, submerged fermentation, and dish culture. T. camphoratus produces different metabolites under different culture methods. In this study, nontargeted metabolomics was used to compare the metabolites of T. camphoratus produced under these four culture methods. Principal component analysis and supervised partial least squares-discriminant analysis were used to analyze the differences in the metabolites. Moreover, in vitro hypoglycemic activity of T. camphoratus extracts produced under four culture methods was compared by assessing their ability to inhibit the activity of α-glucosidase, α-amylase, and sucrase. A total of 186 metabolites were identified. In total, 127 metabolites were common under the four culture methods. Under solid-state fermentation, submerged fermentation, and cutting wood culture, 12, 1, and 4 metabolites were unique, respectively. The differential metabolites produced by T. camphoratus under four culture methods were mainly triterpenoids, phenolic compounds, and fatty acid compounds. α-glucosidase, α-amylase, and sucrase activity inhibition was the best using T. camphoratus extract obtained under cutting wood culture; the inhibition rates were 55.97%, 51.96%, and 78.02%, respectively, which were comparable to those exhibited by 0.001, 3, and 12 mg/mL acarbose (positive control). The metabolites produced by T. camphoratus and α-glucosidase, α-amylase, and sucrase inhibitory activities were different under the four culture methods. Cutting wood culture exhibited the best enzyme inhibitory activity. This study provided a theoretical basis for further use and development of various culture methods for the rational production of active metabolites of T. camphoratus.
Hongen Zhang,Lang Li,Prabir Kumar Sarker,Tao Long,Xiaoshuang Shi,Qingyuan Wang,Gaochuang Cai 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.1
This work quantified the hierarchy of the influence of three common mixture design parameters on the compressive strength and the rate of strength increase over the long term of low-calcium fly ash geopolymer concrete (FAGC) through designing 16 mixtures by the orthogonal experimental design (OED) method. The parameters used in the study were liquid to fly ash (L/FA) ratio, sodium hydroxide concentration (SHC) and sodium silicate solution to sodium hydroxide solution (SS/SH) ratio. The L/FA ratio showed little effect on compressive strength when it was varied from 0.40 to 0.52. SHC showed the greatest influence on compressive strength with little impact on the rate of strength increase after the initial heat curing. Even though the SS/SH ratio showed a small effect on the initial compressive strength, it had a considerable influence on the rate of strength increase over the long term. It was found that the compressive strength at 480 days was positively related to the Na₂O/SiO₂ molar ratio when it was varied from 0.49 to 0.80 and the Si/Al molar ratio was increased up to 1.87. Analysis of the failure types of specimens demonstrated that compressive strength of FAGC was associated with the strength of the mortar–aggregate interface zone (MAIZ).
Facile Synthesis of Zn1-xCuxO Nanorods with a Very Broad Visible Band
Jianguo Lv,Changlong Liu,Wanbing Gong,Zhenfa Zi,Xiaoshuang Chen,Kai Huang,Feng Liu,Tao Wang,Gang He,Xueping Song,Zhaoqi Sun 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.5
Zn1-xCuxO nanorods with different Cu concentrations are prepared by a hydrothermal method. Bent and aggregated nanorods are obtained, which is attributed to centripetal surface tension of the evaporation and coagulation processes of the water film on the ZnO nanorods. The broad visible band consists of one violet, three blue,and one green emission. The violet emission is due to the transition of electrons from zinc interstitial (Zni)levels to the valance band. The three blue emissions may be attributed to the transition from extended Zni levels, which are slightly below the simple Zni level, to the valance band. The change of the green emission may be the result of competition between oxygen vacancies (VO) and zinc vacancies (VZn).