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- 저자 : He Yehua (검색결과 3 건)
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In vitro selection and identifcation of a cold‑tolerant variant in pineapple (Ananas comosus)
Yafen Zhang,Zhiqiang Xu,Tao Xie,Wei Zhang,Yehua He,Chaoyang Liu 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.2
Cold stress is an important factor limiting the growth and distribution of pineapple. Breeding cold-resistant germplasm is an efective way to cope with this problem. In vitro selection for the somaclonal variation using diferent selection agents has been used for crop improvement in stress tolerance. In this study, the pineapple cold-tolerant variant was developed through in vitro cold selection of somaclonal variations. Low temperature was used as the selected agent, and the extreme lethal condition for the in vitro pineapple cultures was determined to be 0 °C for 72 h. The morphology changes of the in vitro cultures during the cold selection were observed and analyzed. The cold-tolerant variant line was fnally obtained through three consecutive selections with cold shock treatments, based on the established high-efciency culture system for pineapple embryogenic calli. The genetic variations at the molecular level in the cold-tolerant variant were verifed by ISSR analysis. The signifcantly improved cold tolerance in our selected variant was mainly refected by the higher survival rate, increased proline content, and elevated SOD activity under cold stress compared to these qualities in the control plants. This study demonstrated the feasibility of in vitro selection for cold tolerance in pineapple. The cold-tolerant variant could be valuable for future pineapple breeding programs and for cold tolerance research.
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Xie Tao,Zhang Wei,Chen Chengjie,Wang Xiaoshuang,Zhang Jing,Luan Aiping,He Yehua 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6
Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifi cally expressed in the early stage of SE in pineapple ( Ananas comosus ), suggesting that the promoter of SERK1 might contain specifi c cis acting element regulating SE. To identify embryonic cell-specifi c element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was signifi cantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specifi c. Besides, a promoter lacking − 943 to -922 was shown to signifi cantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confi rmed again the 5’ upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our fi ndings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.
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