The type II histidine triad protein HtpsC facilitates invasion of epithelial cells by highly virulent Streptococcus suis serotype 2

Lu Yunjun,Li Shu,Shen Xiaodong,Zhao Yan,Zhou Dongming,Hu Dan,Cai Xushen,Lu Lixia,Xiong Xiaohui,Li Ming,Cao Min 한국미생물학회 2021 The journal of microbiology Vol.59 No.10

Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that presents a significant threat both to pigs and to workers in the pork industry. The initial steps of S. suis 2 pathogenesis are unclear. In this study, we found that the type II histidine triad protein HtpsC from the highly virulent Chinese isolate 05ZYH33 is structurally similar to internalin A (InlA) from Listeria monocytogenes, which plays an important role in mediating listerial invasion of epithelial cells. To determine if HtpsC and InlA function similarly, an isogenic htpsC mutant (ΔhtpsC) was generated in S. suis by homologous recombination. The htpsC deletion strain exhibited a diminished ability to adhere to and invade epithelial cells from different sources. Double immunofluorescence microscopy also revealed reduced survival of the ΔhtpsC mutant after cocultivation with epithelium. Adhesion to epithelium and invasion by the wild type strain was inhibited by a monoclonal antibody against E-cadherin. In contrast, the htpsC-deficient mutant was unaffected by the same treatment, suggesting that E-cadherin is the host-cell receptor that interacts with HtpsC and facilitates bacterial internalization. Based on these results, we propose that HtpsC is involved in the process by which S. suis 2 penetrates host epithelial cells, and that this protein is an important virulence factor associated with cell adhesion and invasion.

PEC: A Privacy-Preserving Emergency Call Scheme for Mobile Healthcare Social Networks

Xiaohui Liang,Rongxing Lu,Le Chen,Xiaodong Lin,Xuemin (Sherman) Shen 한국통신학회 2011 Journal of communications and networks Vol.13 No.2

In this paper, we propose a privacy-preserving emergency call scheme, called PEC, enabling patients in life-threatening emergencies to fast and accurately transmit emergency data to the nearby helpers via mobile healthcare social networks (MHSNs). Once an emergency happens, the personal digital assistant (PDA)of the patient runs the PEC to collect the emergency data including emergency location, patient health record, as well as patient physiological condition. The PEC then generates an emergency call with the emergency data inside and epidemically disseminates it to every user in the patient’s neighborhood. If a physician happens to be nearby, the PEC ensures the time used to notify the physician of the emergency is the shortest. We show via theoretical analysis that the PEC is able to provide fine-grained access control on the emergency data, where the access policy is set by patients themselves. Moreover,the PEC can withstandmultiple types of attacks, such as identity theft attack, forgery attack, and collusion attack. We also devise an effective revocation mechanism to make the revocable PEC (rPEC) resistant to inside attacks. In addition, we demonstrate via simulation that the PEC can significantly reduce the response time of emergency care in MHSNs.

Hair follicles transcriptome profiles in Bashang long-tailed chickens with different plumage colors

Xiaohui Liu,Rongyan Zhou,Yongdong Peng,Chuansheng Zhang,Lanhui Li,Chunxiang Lu,Xianglong Li 한국유전학회 2019 Genes & Genomics Vol.41 No.11

Despite the rich variety in plumage color found in nature, genetic studies on how hair follicles affect pigmentation are often limited to animals that have black and white pigment. To test how gene expression influences plumage color, transcriptomes of chicken hair follicles with white, black, hemp, reed catkins, silvery grey, and landscape plumage colors were generated using Illumina sequencing. We generated six RNA-Seq libraries with over 25 million paired-end clean reads per library with percentage of paired-end clean reads ranging from 96.73 to 96.98%. 78% of the reads mapped to the chicken genome, and approximately 70% of the reads were mapped to exons and 6% mapped to introns. Transcriptomes of hair follicles producing hemp and land plumage were similar, but these two showed moderate differences compared with gray and reed colored plumage. The black and white follicle transcriptomes were most divergent from the other colors. We identified several candidate genes, including GPNMB, PMEL, TYRP1, GPR143, OCA2, SOX10, SLC45A2, KRT75, and TYR . All of these genes are known to induce pigment formation in mice. White feathers result from the lack of pigment formation, and our results suggest that the white chickens due to the recessive insertion mutation of TYR . The formation of black area size and color depth may be due to the expression levels of GPNMB, PMEL, TYRP1, GPR143, OCA2, SOX10, SLC45A2, KRT75, and TYR . The GO analysis of the differentially expressed genes (DEGs) revealed that DEGs in our transcriptome analysis were enriched in cytoskeleton and cell structure related pathways. The black plumage transcriptome showed significant differences in melanogenesis, tyrosine metabolism, and riboflavin metabolism compared with transcriptomes of other plumage colors. The transcriptome profiles of the different chicken plumage colors provide a valuable resource to understand how gene expression influences plumage color, and will be an important resource for identifying candidate genes in breeding programs.

PEC: A Privacy-Preserving Emergency Call Scheme for Mobile Healthcare Social Networks

Liang, Xiaohui,Lu, Rongxing,Chen, Le,Lin, Xiaodong,Shen, Xuemin (Sherman) The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.2

In this paper, we propose a privacy-preserving emergency call scheme, called PEC, enabling patients in life-threatening emergencies to fast and accurately transmit emergency data to the nearby helpers via mobile healthcare social networks (MHSNs). Once an emergency happens, the personal digital assistant (PDA) of the patient runs the PEC to collect the emergency data including emergency location, patient health record, as well as patient physiological condition. The PEC then generates an emergency call with the emergency data inside and epidemically disseminates it to every user in the patient's neighborhood. If a physician happens to be nearby, the PEC ensures the time used to notify the physician of the emergency is the shortest. We show via theoretical analysis that the PEC is able to provide fine-grained access control on the emergency data, where the access policy is set by patients themselves. Moreover, the PEC can withstandmultiple types of attacks, such as identity theft attack, forgery attack, and collusion attack. We also devise an effective revocation mechanism to make the revocable PEC (rPEC) resistant to inside attacks. In addition, we demonstrate via simulation that the PEC can significantly reduce the response time of emergency care in MHSNs.

A Multi-Criteria QoS-aware Trust Service Composition Algorithm in Cloud Computing Environments

Weina Lu,Xiaohui Hu,Shangguang Wang,Xiaotao Li 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.1

The molybdenum species of MoO3/SiO2 and their catalytic activities for the epoxidation of propylene with cumene hydroperoxide

Yongxia Miao,Xiaohui Liu,Yanglong Guo,Yanqin Wang,Yun Guo,Guanzhong Lu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.1

The MoO3/SiO2 catalysts containing different surface molybdenum species were prepared by a sol–gel method, and the effects of the preparation condition and MoO3 loading on the surface molybdenum species and property of MoO3/SiO2 were studied. The XRD, FT-IR, UV–vis and Raman spectroscopies were used to characterize the surface molybdenum species, and temperature-programmed desorption of NH3 adsorbed on a catalyst was used to detect the surface acidic properties. The results show that, therewere the dispersed polymolybdate, a-MoO3, b-MoO3, monomeric molybdenum species and silicomolybdic acid on the MoO3/SiO2 catalyst, and their distributions and subsistence states were affected by the preparation condition and MoO3 loading. Different molybdenum species exhibit different catalytic activities for the epoxidation of propylene with cumene hydroperoxide. In the 15 wt% MoO3/SiO2 catalyst synthesized at pH 9.1 and dried appropriately, there are the small size b-MoO3 and monomeric molybdenum species that they are mainly effective catalyst components for the epoxidation of propylene. Using this catalyst, the ~100% conversion of cumene hydroperoxide and ~100% selectivity to propylene oxide can be obtained in the tert-butyl alcohol solvent at 2.6 MPa and 80 8C for 4 h.

High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/β-catenin pathway

Shichao Zhuang,Xiaohui Yu,Ming Lu,Yujiao Li,Ning Ding,Yumei Ding 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.6

Objective: High mobility group box 3 (HMGB3) plays an important role in the developmentof various cancer. This study aims to explore whether HMGB3 regulates cervical cancer (CC)progression and elucidate the underlying mechanism. Methods: HMGB3 expression in clinical patients' tumor samples were determined byreal-time quantitative polymerase chain reaction (qRT-PCR) and western blot. HMGB3overexpression/knockdown were used to investigate its function. Cell apoptosis and cyclewere detected by Annexin V/PI staining and flow cytometry. In vivo tumor model was madeby subcutaneous injection of HeLa cells transfected with shRNAs targeting HMGB3 (sh HMGB31) into the flank area of nude mice. Western blot was used to detect the levels ofβ-catenin, c-Myc, and matrix metalloproteinase-7 (MMP-7) in Hela and CaSki cells transfectedwith sh-HMGB3 or shRNAs targeting β-catenin. Results: Both messenger RNA and protein levels of HMGB3 were upregulated in CC tissuesfrom patients. High expression level of HMGB3 had positive correlation with serosalinvasion, lymph metastasis, and tumor sizes in CC patient. Functional experiments showedthat HMGB3 could promote CC cell proliferation both in vitro and in vivo. The expressionlevels of c-Myc and MMP-7 were increased, resulting in regulating cell apoptosis, cell cycle,and activating Wnt/β-catenin pathway. Conclusions: Our data indicated that HMGB3 may serve as an oncoprotein. It could be used asa potential prognostic marker and represent a promising therapeutic strategy for CC treatment.

A Metadata-based Method for Sharing Multiply Heterogeneous Information

Xiaotao Li,Xiaohui Hu,Weina Lu,Xi Liu 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.3

As users’ requirements for information integration enhance increasingly, how to integrate multiply heterogeneous data in a global sharing system has especially been a challenge for its large scale and diverse formats. To address the above problem, this paper proposes an information sharing approach for multiply heterogeneous data based on a two-layer metadata. Firstly, the architecture of the two-layer metadata is introduced. Secondly, the synchronization between different users for distributed heterogeneous data is realized by sharing table structures. Finally, Lucene search engine combined with the element GM-description of the two-layer metadata is presented to retrieve metadata, which reduces the response time compared to other retrieval methods. The experiment results illustrate the effectiveness of our approach and the conclusion is given.

Secrecy Spectrum and Secrecy Energy Efficiency in Massive MIMO Enabled HetNets

( Zhihao Zhong ),( Jianhua Peng ),( Kaizhi Huang ),( Lu Xia ),( Xiaohui Qi ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.2

Security and resource-saving are both demands of the fifth generation (5G) wireless networks. In this paper, we study the secrecy spectrum efficiency (SSE) and secrecy energy efficiency (SEE) of a K-tier massive multiple-input multiple-output (MIMO) enabled heterogeneous cellular network (HetNet), in which artificial noise (AN) are employed for secrecy enhancement. Assuming (i) independent Poisson point process model for the locations of base stations (BSs) of each tier as well as that of eavesdroppers, (ii) zero-forcing precoding at the macrocell BSs (MBSs), and (iii) maximum average received power-based cell selection, the tractable lower bound expressions for SSE and SEE of massive MIMO enabled HetNets are derived. Then, the influences on secrecy oriented spectrum and energy efficiency performance caused by the power allocation for AN, transmit antenna number, number of users served by each MBS, and eavesdropper density are analyzed respectively. Moreover, the analysis accuracy is verified by Monte Carlo simulations.

Two-Dimensional SPH Analysis of Seepage with Water Injection Process for Different Crack Morphologies

Haichun Ma,Jingping Wang,Jiazhong Qian,Xiaohui Tan,Luwang Chen,Yaping Deng,Zhitang Lu,Lei Ma 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

Crack seepage in nature involves natural and water injection flow, causing crack morphology in nature to be varied. Using the numerical simulation method of smoothed particle hydrodynamics (SPH), the natural free flow process in a crack was analyzed. With the interference of the initial velocity of the x = 0 particles, the other terminal particles were shifted, and the particles with initial velocities were reversed. For water injection simulations, ghost particles were set to block particles from overflowing, as particles subjected to injection water were mobilized more quickly than under free flow conditions. For rough cracks, particle magnitude was affected by the boundary shape, but overall particle velocity was influenced little, and the distribution of particles was sparse and densely spaced. For the branch crack simulation, overall particle seepage velocity decreased, but particles moved faster through the wider branch than through the narrower branch. SPH can simulate the seepage through crack networks and be used to analyze the effects of different crack geometries and physical properties.