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Xia Qian,Zhan Gaofeng,Mao Meng,Zhao Yin,Li Xing 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Excessive and unresolved neuroinflammation is a key component of the pathological cascade in brain injuries such as ischemic stroke. Tripartite motif-containing 45 (TRIM45) is a ubiquitin E3 ligase involved in various critical biological processes. However, the role of TRIM45 in cerebral ischemia remains unknown. Here, we found that the TRIM45 protein was highly expressed in the peri-infarct areas of mice subjected to cerebral ischemia and reperfusion injury induced by middle cerebral artery occlusion. This study systemically evaluated the putative role of TRIM45 in the regulation of neuroinflammation during ischemic injury and the potential underlying mechanisms. We found that TRIM45 knockdown significantly decreased proinflammatory cytokine and chemokine production in primary cultured microglia challenged with oxygen-glucose deprivation and reoxygenation (OGD/R) treatment. Mechanistically, we demonstrated that TRIM45 constitutively interacted with TAB2 and consequently facilitated the Lys-63-linked polyubiquitination of TAB2, leading to the formation of the TAB1–TAK1–TAB2 complex and activation of TAK1, which was ultimately followed by activation of the nuclear factor-kappa B (NF-κB) signaling pathway. In an in vitro coculture Transwell system, downregulation of TRIM45 expression also inhibited the OGD/R-induced activation of microglia and alleviated neuronal apoptosis. More importantly, microglia-specific knockdown of TRIM45 in mice significantly reduced the infarct size, mitigated neurological deficit scores, and improved cognitive function after ischemic stroke. Taken together, our study reveals that the TRIM45–TAB2 axis is a crucial checkpoint that controls NF-κB signaling in microglia during cerebral ischemia and reperfusion injury. Therefore, targeting TRIM45 may be an attractive therapeutic strategy.
Qian, Zhi‐,Gang,Xia, Xiao‐,Xia,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2011 Biotechnology and bioengineering Vol.108 No.1
<P><B>Abstract</B></P><P>A five carbon linear chain diamine, cadaverine (1,5‐diaminopentane), is an important platform chemical having many applications in chemical industry. Bio‐based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum‐based chemical synthesis. Here, we report development of a metabolically engineered strain of <I>Escherichia coli</I> that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, <SMALL>L</SMALL>‐lysine decarboxylase, which converts <SMALL>L</SMALL>‐lysine directly to cadaverine, was amplified by plasmid‐based overexpression of the <I>cadA</I> gene under the strong <I>tac</I> promoter. Furthermore, the <SMALL>L</SMALL>‐lysine biosynthetic pool was increased by the overexpression of the <I>dapA</I> gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong <I>trc</I> promoter in the genome. The final engineered strain was able to produce 9.61 g L<SUP>−1</SUP> of cadaverine with a productivity of 0.32 g L<SUP>−1</SUP> h<SUP>−1</SUP> by fed‐batch cultivation. The strategy reported here should be useful for the bio‐based production of cadaverine from renewable resources. Biotechnol. Bioeng. 2011; 108:93–103. © 2010 Wiley Periodicals, Inc.</P>
High-performance Zinc-Tin-Oxide thin film transistors based on environment friendly solution process
Qian Zhang,Guodong Xia,Lubin Li,Wenwen Xia,Hong-Yu Gong,Sumei Wang 한국물리학회 2019 Current Applied Physics Vol.19 No.2
Zinc-Tin-Oxide (ZTO) thin films were fabricated using a simple and eco-friendly sol-gel method and their application in thin film transistors (TFTs) was investigated. Annealing temperature has a crucial influence on the structure and electrical properties of sol-gel ZTO thin films. The ZTO thin films annealed at 300–600 °C revealed smooth and uniform surfaces with amorphous state, in addition, a high optical transparency over 90% of the ZTO films in the visible range was obtained. The electrical performance of ZTO TFTs showed obvious dependence on annealing temperature. The ZTO TFTs annealed at 500 °C showed a high carrier mobility of 5.9 cm2 /V, high on/off current ratio (Ion/off) of 106 -107 , and threshold voltage (Vth) of 1.03 V. To demonstrate the application of sol-gel ZTO films in low-power display fields, we also fabricated ZTO TFTs with a solution-processed high-permittivity (high-k) ZrTiOx dielectric layer. The ZTO/ZrTiOx TFTs showed high mobility of 17.9 cm2 /V and Ion/off of 105 -106 at a low operation voltage of 3 V, indicating that Indium-free ZTO thin films would be potential candidates for low cost, high performance oxide TFT devices.
Method of Identifying Buck Converter Load Without Load Current Measurements
Xia Qian,Yue Jiguang,Wu Chenhao,Lyu Feng 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2
This paper proposes a novel method to identify the load without measuring the load current. The variation of load current in the Buck converter affects the feedback performance of the converter, influencing the maintenance of steady output voltage. To ensure the long-period and high-stability operation of the Buck converter. This paper investigates the monitoring of load changes without extra measurement sensors in the original topology. This method mainly utilizes load regulation characteristics in the Buck converter load regulation. When it is necessary to monitor the load condition, the "plug-in" observer will be connected in parallel with the load. Thus the load can be identified by measuring the voltage and bypass current through the external observer. Compared with existing identification methods for converter load, this method avoids measuring the internal parameters and variables inside the converter but only measures the output voltage and bypass current on the output side. The identification values can also be applied to determine the operating status of the converter and further predict the possible fault and failure aroused from the load. In conclusion, SIMETRIX simulations and experiments for three switching converter products under different power levels can validate the proposed method.
Towards Competence-based Translation Curricular Reform for Translation Education Programmes in China
Qian XIA 한국외국어대학교 통번역연구소 2019 한국외국어대학교 통번역연구소 학술대회 Vol.2019 No.07
With the ever increasing globalization and interdependencies in economic development as well as economic development, China is in growing demand for qualified translators and interpreters. And this calls for the supply of translation education programmes at university level. With its launch in 2007, China’s Master in Translation and Interpreting (MTI) programme has seen its dramatic growth over the past decade. However, what an MTI programme should aim for has remained a question under debate. The paper reviews the discipline context of Translation Studies as well as the history and recent development of translation programmes in China. The paper also examines the justification of underlying curriculum reform with the inclusion of intercultural communicative competence (ICC) as one of the core modules for MTI. And this will be done by the review and introduction of multiple well developed research projects/models and particular emphasis will be put on the review of a project promoting intercultural communicative competence for translation students. It concludes recent work from pedagogical /education oriented translation competence, arguing that a more holistic understanding of the translation curriculum must be reflected in series of the narrow, jobspecific- competence-based training programme.
Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine
Qian, Zhi-Gang,Xia, Xiao-Xia,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.104 No.4
<P>A four carbon linear chain diamine, putrescine (1,4-diaminobutane), is an important platform chemical having a wide range of applications in chemical industry. Biotechnological production of putrescine from renewable feedstock is a promising alternative to the chemical synthesis that originates from non-renewable petroleum. Here we report development of a metabolically engineered strain of Escherichia coli that produces putrescine at high titer in glucose mineral salts medium. First, a base strain was constructed by inactivating the putrescine degradation and utilization pathways, and deleting the ornithine carbamoyltransferase chain I gene argI to make more precursors available for putrescine synthesis. Next, ornithine decarboxylase, which converts ornithine to putrescine, was amplified by a combination of plasmid-based and chromosome-based overexpression of the coding genes under the strong tac or trc promoter. Furthermore, the ornithine biosynthetic genes (argC-E) were overexpressed from the trc promoter, which replaced the native promoter in the genome, to increase the ornithine pool. Finally, strain performance was further improved by the deletion of the stress responsive RNA polymerase sigma factor RpoS, a well-known global transcription regulator that controls the expression of ca. 10% of the E. coli genes. The final engineered E. coli strain was able to produce 1.68 g L<SUP>−1</SUP> of putrescine with a yield of 0.168 g g<SUP>−1</SUP> glucose. Furthermore, high cell density cultivation allowed production of 24.2 g L<SUP>−1</SUP> of putrescine with a productivity of 0.75 g L<SUP>−1</SUP> h<SUP>−1</SUP>. The strategy reported here should be useful for the bio-based production of putrescine from renewable resources, and also for the development of strains capable of producing other diamines, which are important as nitrogen-containing platform chemicals. Biotechnol. Bioeng. 2009; 104: 651–662 © 2009 Wiley Periodicals, Inc.</P>