Efficient BP Neural Network based De-interlacing Algorithm

Jin Wang,Gwanggil Jeon,Jiaji Wu,Jechang Jeong 한국통신학회 2014 한국통신학회 학술대회논문집 Vol.2014 No.6

Ni–Au core–shell nanowires: synthesis, microstructures, biofunctionalization, and the toxicological effects on pancreatic cancer cells

Jeon, In Tak,Cho, Moon Kyu,Cho, Jin Woo,An, Boo Hyun,Wu, Jun Hua,Kringel, Rosemarie,Choi, Daniel S.,Kim, Young Keun Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.32

<P>The nickel (Ni)–gold (Au) core–shell nanowires (CSNWs) were synthesized by a combination of electrodeposition and electroless-plating methods. This template based, electroless-plating approach can control the size and shape of nanostructures by adjusting the experimental conditions. The X-ray diffraction, transmission electron microscopy (TEM), and TEM line-scanning and elemental mapping analyses were applied to investigate the formation and microstructure of the core–shell nanostructure. Also using a focused ion beam lift-out technique provides an important route to prove the inner microstructural details of the CSNWs. The optical characterization showed the surface plasmon absorption shifting with electroless-plating time, whereas the magnetic measurements revealed the shape anisotropy and soft ferromagnetic properties of the NWs. Using the strong interaction between biotin and streptavidin, streptavidin–fluorescent dyes were successfully conjugated on the biotinylated CSNWs. This was then analyzed for the surface plasmon resonance effect and Stokes' shifting <I>via</I> ultraviolet-visible and photoluminescence spectroscopy measurements and imaged by confocal scanning laser microscopy. Proliferating cancer cells (Panc-1) have been used to study the toxicological effects of both Ni–Au CSNWs and Ni NWs in a living cell system: the cellular responses to nanowire effects were observed. Ni–Au CSNWs appeared to be tolerated more by this cell line at the dose tested than Ni NWs.</P> <P>Graphic Abstract</P><P>Bio-applicable Ni–Au core–shell magnetic nanowire prepared from the combined electrodeposition and electroless-plating and thoroughly characterized for therapeutic applications. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm11143d'> </P>

High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) SUMOylation by the SUMO E3 Ligase PIAS1 Decreases the Binding Affinity to Nucleosome Core Particles

Wu, Jie,Kim, Sol,Kwak, Man Sup,Jeong, Jang Bin,Min, Hyun Jin,Yoon, Ho-Geun,Ahn, Jin-Hyun,Shin, Jeon-Soo American Society for Biochemistry and Molecular Bi 2014 The Journal of biological chemistry Vol.289 No.29

<P>High mobility group nucleosomal binding domain 2 (HMGN2) is a small and unique non-histone protein that has many functions in a variety of cellular processes, including regulation of chromatin structure, transcription, and DNA repair. In addition, it may have other roles in antimicrobial activity, cell homing, and regulating cytokine release. Although the biochemical properties of HMGN2 protein are regulated by acetylation and phosphorylation, it is not yet known whether HMGN2 activity can also be regulated by SUMOylation. In this study, we demonstrated for the first time that HMGN2 is modified by covalent attachment of small ubiquitin-related modifier 1 (SUMO1) by pro-inflammatory signal and identified the major SUMOylated lysine residues that localize to the HMGN2 nucleosome-binding domain at Lys-17 and Lys-35. SENP1 can deSUMOylate SUMOylated HMGN2, and PIAS1 is the E3 ligase responsible for SUMOylation of HMGN2. Finally, using SUMO1-conjugated HMGN2 purified from a basal SUMOylation system in <I>Escherichia coli</I>, we demonstrated that SUMOylated HMGN2 has decreased the binding affinity to nucleosome core particles in comparison to unSUMOylated HMGN2. These observations potentially provide new perspectives for understanding the functions of HMGN2 in inflammatory reaction.</P>

Effects of Cold Stress on Transcripts and Metabolites in Tartary Buckwheat (Fagopyrum tataricum)

Jin Jeon,Jae Kwang Kim,Qi Wu,Sang Un Park 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

Bilateral Filtering and Directional Differentiation for Bayer Demosaicking

Wang, Jin,Wu, Jiaji,Wu, Zhensen,Jeon, Gwanggil,Jeong, Jechang IEEE 2017 IEEE SENSORS JOURNAL Vol.17 No.3

<P>In this paper, we introduce an efficient image demosaicking method using a bilateral filter and directional differentiation with consideration of both the spatial closeness and the similarity between the interpolated pixel and the neighbor pixels. Spatial closeness is considered as spatial locality. We utilize an adaptive weighted average to estimate the missing pixel value, where the adaptive weight is calculated based on three components: directional differentiation, similarity between the pixel and each of its neighbor pixels, and spatial locality. The experimental results show that the proposed method outperforms existing approaches in both objective and subjective performance.</P>

Voice Coil Navigation Sensor for Flexible Silicone Intubation

Fang Wu,Jiyun Jeon,Seung Ki Moon,Hae-Jin Choi,Hungsun Son IEEE 2016 IEEE/ASME transactions on mechatronics Vol.21 No.2

<P>This paper presents a novel navigation sensor for guidance of a flexible tube based on magnetic induction. In many applications, such as pipe inspection and medical intubation, the flexible tube has been utilized for inner surface monitoring and inspection. In particular, for medical intubation that inserts a stomach tube or endoscope, accuracy of locating the flexible tube mainly relies on experienced operators. Although X-ray tomography and oxygen sensors have been applied to detect the location of the inserted tube, the implementation is usually time consuming and costly, an equipment being bulky so that their practical applications are limited. On the contrary, magnetic sensors, offering contact free and compactness in size, have been preferred in the areas of position/orientation detection. In this study, a compact orientation sensor based on magnetic induction has been developed; both magnetic field generator and detector are designed to minimize the space along the flexible tube. Magnetic mutual inductance is analyzed with the extended-distributed multiple-pole model, and design parameters are optimized to determine an operating range of orientation angles. Performance of the sensor is numerically simulated and compared to experimental results. The results show the accuracy in a wide operating range and the potential in many applications.</P>

Superior Treatment Response and In-field Tumor Control in Epidermal Growth Factor Receptor-mutant Genotype of Stage III Nonsquamous Non–Small cell Lung Cancer Undergoing Definitive Concurrent Chemoradiotherapy

Lim, Yu Jin,Chang, Ji Hyun,Kim, Hak-Jae,Keam, Bhumsuk,Kim, Tae Min,Kim, Dong-Wan,Paeng, Jin Chul,Kang, Keon Wook,Chung, June-Key,Jeon, Yoon Kyung,Chung, Doo Hyun,Wu, Hong-Gyun Elsevier 2017 Clinical lung cancer Vol.18 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Although previous <I>in vitro</I> data have suggested a more radio-sensitive nature of epidermal growth factor receptor (EGFR)-mutant non–small cell lung cancer (NSCLC) cell lines, the clinical behavior according to the EGFR mutational status has not been well-established. In this study, we performed a comparative outcome analysis of EGFR-mutant and wild-type locally advanced NSCLC with chemoradiotherapy (CRT).</P> <P><B>Patients and Methods</B></P> <P>A total of 102 patients with stage III nonsquamous NSCLC undergoing primary CRT were identified. Clinicopathologic characteristics, including the degree of glucose uptake, were evaluated. Failure patterns considering the radiation field and survival outcomes were compared according to the EGFR mutational status.</P> <P><B>Results</B></P> <P>Pre- and post-CRT maximum standardized uptake values were significantly lower in EGFR-mutant tumors (<I>P</I> = .010 and .018, respectively). The overall response rate was higher in the EGFR-mutant group compared with the wild-type (89% vs. 64%, respectively; <I>P</I> = .023). The 3-year overall survival rate was better with the genetic alteration (68.0% vs. 47.4%, <I>P</I> = .046), but the statistical significance did not remain in multivariate analysis (hazard ratio, 0.68; 95% confidence interval, 0.30-1.55). Considering the tumor progression inside or outside the radiation field, the EGFR-mutant group showed longer in-field time to progression (<I>P</I> = .002), even after adjusting for other related baseline variables (hazard ratio, 0.27; 95% confidence interval, 0.11-0.71).</P> <P><B>Conclusion</B></P> <P>The differential metabolic activity, failure patterns, and prognosis suggest the distinct nature of the EGFR-mutant tumors. EGFR mutational status needs to be considered for more precise curative-intent treatment strategies of locally advanced nonsquamous NSCLC.</P>

A modified transient gene expression protocol for subcellular protein localization analysis in rice

Yiming Wang,Jingni Wu,Sang Gon Kim,Ju Soon Yoo,Ravi Gupta,제병일,Jong‑Seong Jeon,Ki‑Hong Jung,Yu‑Jin Kim,KyuYoungKang,김선태 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Transient protein expression is a useful technique for investigating the protein localization and functional analysis of stress responses in rice plants. Currently, available methods for in planta transient expression analysis include Agrobacteriummediated transformation, protoplast transformation using polyethylene glycol or electroporation, and biolistic bombardment expression which have several disadvantages and are not well suited for the rice. Therefore, development of a method for rapid and efficient analysis of protein expression, subcellular localization, pathogen effector screening, and protein–protein interaction in rice is required. We developed a protocol for in planta gene expression analysis in sliced rice sheath cells by modifying and optimizing the biolistic particle bombardment technique. By obtaining thin sections (~400 μm) of rice sheath cells, auto-fluorescence from chlorophyll was eliminated. This system was validated through the localization of marker genes specifically expressed in nuclei, plasma membranes, and tonoplast. In addition, high transformation efficiency of 30% was achieved. Therefore, this protocol provides a new and rapid method for transient gene expression assay in rice. Protein secretion was examined in rice sheath cells using predicted secretory proteins from rice blast fungus, indicating that this method is applicable to plant–microbe interaction studies. The transient expression protocol established here is well optimized for protein localization, secretion, and host–pathogen protein interaction studies in rice. A typical experiment can be completed in three days.

Modeling, Design, Fabrication, and Demonstration of a Digital Micromirror With Interdigitated Cantilevers

Dae-Hyun Kim,Min-Wu Kim,Jin-Wan Jeon,Koeng Su Lim,Jun-Bo Yoon IEEE 2009 Journal of microelectromechanical systems Vol.18 No.6

<P>We present the modeling, design, fabrication, and measurement results of a novel digital micromirror based on a new actuator called interdigitated cantilevers. In contrast to conventional micromirrors that rotate through the twisting actuation of a hinge, this micromirror has a symmetric bidirectional rotation through a bending actuation of interdigitated cantilevers hidden under a mirror plate. For the static and dynamic characteristics of the proposed micromirror, analytical models were developed first on the basis of the Euler-Bernoulli beam equation, as well as both distributed and lumped-parameter models. The results of the developed analytical models are in good agreement with those of a finite-element-method (FEM) simulation, having just a 10% deviation. On the basis of these analytical models, we successfully designed, fabricated, and evaluated a micromirror with a mirror size of 16 mum times 16 mum. The fabricated micromirror has a mechanical rotation angle of plusmn10deg, a pull-in voltage of 54 V, a resonant frequency of 350 kHz, and a switching response time of 17 mus. The measurement results compare favorably with those of analytical models and FEM simulations, with deviations of less than 15% and 10%, respectively.</P>

Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model

Youn, Ju Ho,Kwak, Man Sup,Wu, Jie,Kim, Eun Sook,Ji, Yeounjung,Min, Hyun Jin,Yoo, Ji-Ho,Choi, Ji Eun,Cho, Hyun-Soo,Shin, Jeon-Soo WILEY-VCH Verlag 2011 European journal of immunology Vol.41 No.9

<P>Lipopolysaccharide (LPS) triggers deleterious systemic inflammatory responses when released into the circulation. LPS-binding protein (LBP) in the serum plays an important role in modifying LPS toxicity by facilitating its interaction with LPS signaling receptors, which are expressed on the surface of LPS-responsive cells. We have previously demonstrated that high mobility group box 1 (HMGB1) can bind to and transfer LPS, consequently increasing LPS-induced TNF-α production in human peripheral blood mononuclear cells (PBMCs). We report here on the identification of two LPS-binding domains within HMGB1. Furthermore, using 12 synthetic HMGB1 peptides, we define the LPS-binding regions within each domain. Among them, synthetic peptides HPep1 and HPep6, which are located in the A and B box domains of HMGB1, bind to the polysaccharide and lipid A moieties of LPS respectively. Both HPep1 and HPep6 peptides inhibited binding of LPS to LBP and HMGB1, LBP-mediated LPS transfer to CD14, and cellular uptake of LPS in RAW264.7 cells. These peptides also inhibited LPS-induced TNF-α release in human PBMCs and induced lower levels of TNF-α in the serum in a subclinical endotoxemia mouse model. These results indicate that HMGB1 has two LPS-binding peptide regions that can be utilized to design anti-sepsis or LPS-neutralizing therapeutics.</P>