곡류가공이 돼지성장과 도체특성에 미치는 영향

WU.JIH-FANG,WU. JIH-FANG 대한양돈협회 1990 養豚 Vol.12 No.10

Propamidine decreas mitochondrial complex 3 activity of Botrytis cinerea

( Fangli Wu ),( Weibo Jin ),( Juntao Feng ),( Anliang Chen ),( Zhiqing Ma ),( Xing Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.9

Propamidine, an aromatic diamidine compound, is widely used as an antimicrobial agent. To uncover its mechanism on pathogenetic fungi, Botrytis cinerea as an object was used to investigate effects of propamidine in this paper. The transmission electron microscope results showed that the mitochondrial membranes were collapsed after propamidine treatment, followed that mitochondria were disrupted. Inhibition of whole-cell and mitochondrial respiration by propamidine suggested that Propamidine is most likely an inhibitor of electron transport within Botrytis cinerea mitochondria. Furthermore, the mitochondrial complex 3 activity were inhibited by propamidine. [BMB reports 2010; 43(9): 614-621]

Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China

Fang, Yuan,Li, Bin,Wang, Fang,Liu, Baoping,Wu, Zhiyi,Su, Ting,Qiu, Wen,Xie, Guanlin The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4

An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.

Voice Coil Navigation Sensor for Flexible Silicone Intubation

Fang Wu,Jiyun Jeon,Seung Ki Moon,Hae-Jin Choi,Hungsun Son IEEE 2016 IEEE/ASME transactions on mechatronics Vol.21 No.2

<P>This paper presents a novel navigation sensor for guidance of a flexible tube based on magnetic induction. In many applications, such as pipe inspection and medical intubation, the flexible tube has been utilized for inner surface monitoring and inspection. In particular, for medical intubation that inserts a stomach tube or endoscope, accuracy of locating the flexible tube mainly relies on experienced operators. Although X-ray tomography and oxygen sensors have been applied to detect the location of the inserted tube, the implementation is usually time consuming and costly, an equipment being bulky so that their practical applications are limited. On the contrary, magnetic sensors, offering contact free and compactness in size, have been preferred in the areas of position/orientation detection. In this study, a compact orientation sensor based on magnetic induction has been developed; both magnetic field generator and detector are designed to minimize the space along the flexible tube. Magnetic mutual inductance is analyzed with the extended-distributed multiple-pole model, and design parameters are optimized to determine an operating range of orientation angles. Performance of the sensor is numerically simulated and compared to experimental results. The results show the accuracy in a wide operating range and the potential in many applications.</P>

Dimensionality of Augmented Reality Technological Attributes in the Context of Fashion Retailing

Wu, Yi Fang,Kim, Eun Young 한국생활과학회 2021 한국생활과학회 학술대회 Vol.2021 No.11

Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography

Fang Liu,Ni Ma,Fang-Bo Xia,Peng Li,Chengwei He,Zhenqiang Wu,Jian-Bo Wan 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities. Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography. Results: The dried PNL powder was immersed in the distilled water at 50C for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column. Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.

Magnetic Resonance Image Manifestations of the Atypical Meningioma

Wu, Qing-Wu,Yan, Rui-Fang,Li, Qiang,Hu, Ying,Zhou, Feng-Mei,Ren, Ji-Peng,Yang, Rui-Min,Zhang, Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Through retrospective analysis of 13 cases of magnetic resonance image (MRI) manifestations of atypical meningiomas confirmed by operation and pathology in the First Affiliated Hospital of Xinxiang Medical University, the objective of this study was to evaluate the diagnostic value of MRI in order to improve the accuracy rate of preoperative diagnosis. In this retrospective analysis of MRI findings for atypical meningiomas in First Affiliated Hospital of Xinxiang Medical University from January to July in 2012, the location, morphology and tumor signals and other tumor imaging characteristics were covered. In 13 cases of atypical meningioma patients of this group, most tumors were located at typical sites (10/13), mainly the falx cerebri, parasagittal, convexity, saddle area. Only two cases were at atypical locations, 1 in the cerebellar hemisphere and 1 in a lateral ventricle. Most of the tumors showed T1 and T2 isointensity signals, and necrosis, calcification, and peritumoral edema were always featured. DWI showed isointensity in 11 cases (11/13), and hyperintensity in 2. Some 9 cases had dural tail signs, 12 had accurate positioning (12/13), and 2 were postoperative recurrences. MRI has high value in the diagnosis of atypical meningiomas, with important roles in early clinical diagnosis, treatment and prognosis evaluation.

Performance of water-jet pump under acceleration

Wu Xian-Fang,Li Ming-Hui,Liu Houlin,Tan Minggao,Lu You-Dong 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

The instantaneous acceleration affects the performance of the water-jet pump obviously. Here, based on the user-defined function, the method to simulate the inner flow in water-jet pumps under acceleration conditions was established. The effects of two different acceleration modes (linear acceleration and exponential acceleration) and three kinds of different acceleration time (0.5s, 1s and 2s) on the performance of the water-jet pump were analyzed. The results show that the thrust and the pressure pulsation under exponential acceleration are lower than that under linear acceleration at the same time; the vapor volume fraction in the impeller under linear acceleration is 27.3% higher than that under exponential acceleration. As the acceleration time increases, the thrust gradually increases and the pressure pulsation amplitude at the impeller inlet and outlet gradually decreases, while the law of pressure pulsation is the opposite at the diffuser outlet. The main frequency of pressure pulsation at the impeller outlet is different under different acceleration time. The research results can provide some reference for the optimal design of water-jet pumps.

Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiang-Mei,Liu, Xing,Jiao, Qing-Fang,Fu, Shao-Yue,Bu, You-Quan,Song, Fang-Zhou,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

Preparation and Thermoelectric Properties of Flower-Like Nanoparticles of Ce-Doped Bi2Te3

Fang Wu,Wenyu Shi,Xing Hu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.1

Ce-doped CexBi2-xTe3 (x = 0, 0.1, 0.2, 0.3) flower-like nanoparticles were synthesizedby a hydrothermal method through a careful adjustment of the amount ofethylenediamine tetraacetic acid surfactant. The nanopowders of flower-likenanoparticles were hot-pressed into bulk pellets and the thermoelectric properties ofthe pellets examined. The results showed that Ce-doping increased the power factoreffectively at low temperature; however, the thermal conductivity of the nanopowdertoo increased because of the lighter atomic mass of Ce compared to Bi. As a result, aZT value of 1.22, higher than that of the undoped sample, was attained at 386 K forthe Ce0.1Bi1.9Te3 sample.