http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Caspase-3 inhibitor inhibits enterovirus D68 production
Wenbo Huo,Jinghua Yu,Chunyu Liu,Ting Wu,Yue Wang,Xiangling Meng,Fengmei Song,Shuxia Zhang,Ying Su,Yumeng Liu,Jinming Liu,Xiaoyan Yu,Shucheng Hua 한국미생물학회 2020 The journal of microbiology Vol.58 No.9
Enterovirus D68 (EVD68) is an emerging pathogen that recently caused a large worldwide outbreak of severe respiratory disease in children. However, the relationship between EVD68 and host cells remains unclear. Caspases are involved in cell death, immune response, and even viral production. We found that caspase-3 was activated during EVD68 replication to induce apoptosis. Caspase-3 inhibitor (Z-DEVDFMK) inhibited viral production, protected host cells from the cytopathic effects of EVD68 infection, and prevented EVD68 from regulating the host cell cycle at G0/G1. Meanwhile, caspase-3 activator (PAC-1) increased EVD68 production. EVD68 infection therefore activates caspase-3 for virus production. This knowledge provides a potential direction for the prevention and treatment of disease related to EVD68.
Nonlinear Predictive Control of Active Four-wheel Steering Vehicles
Shuyou Yu,Wenbo Li,Baojun Lin,Yongfu Li,Hong Chen,Hongqing Chu,Jianhua Yu 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10
In order to improve the handling stability of active four-wheel steering vehicles, a nonlinear model predictive controller is presented, which can guarantee that the actual sideslip angle and yaw rate can track the ideal sideslip angle and the ideal yaw rate through control of the front and rear wheel angles. A nonlinear static tyre model connected with a linear dynamic model is adopted to describe the vehicle dynamics. Furthermore, the tyre model is replaced by a map in the optimization problem of nonlinear model predictive control. The introduction of maps can reduce the online computational time by a trade-off between the computational burden of CPU and the storage burden of ROM. Simulation results in CarSim indicate that the proposed controller can follow the outputs of the ideal reference model, reduce the computational burden, and improve the handling stability of the active four-wheel steering vehicles effectively.
Wenbo Zhou,Huiyan Wang,Yuqi Yang,Fang Guo,Bin Yu,Zhaoliang Su 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.5
Trophoblasts, important functional cells in the placenta, play a critical role in maintaining placental function. The heterogeneity of trophoblasts has been reported, but little is known about the trophoblast subtypes and distinctive functions during preeclampsia (PE). In this study, we aimed to gain insight into the cell type-specific transcriptomic changes by performing unbiased single-cell RNA sequencing (scRNA-seq) of placental tissue samples, including those of patients diagnosed with PE and matched healthy controls. A total of 29,006 cells were identified in 11 cell types, including trophoblasts and immune cells, and the functions of the trophoblast subtypes in the PE group and the control group were also analyzed. As an important trophoblast subtype, extravillous trophoblasts (EVTs) were further divided into 4 subgroups, and their functions were preliminarily analyzed. We found that some biological processes related to pregnancy, hormone secretion and immunity changed in the PE group. We also identified and analyzed the regulatory network of transcription factors (TFs) identified in the EVTs, among which 3 modules were decreased in the PE group. Then, through in vitro cell experiments, we found that in one of the modules, CEBPB and GTF2B may be involved in EVT dysfunction in PE. In conclusion, our study showed the different transcriptional profiles and regulatory modules in trophoblasts between placentas in the control and PE groups at the single-cell level; these changes may be involved in the pathological process of PE, providing a new molecular theoretical basis for preeclamptic trophoblast dysfunction.
A novel flexible micro-ratchet/ZnO nano-rods surface with rapid recovery icephobic performance
왕만상,Wenbo Yu,Yupei Zhang,우재영,Yucheng Du,Bo Wang,Yanbin Yun,유계성,이중기,Lei Wang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.62 No.-
In this communication, we present a significant method to fabricate a robust icephobic surface on a flexible polymer substrate. The flexible functional surface is obtained by integrating both soft-lithography and crystal growth methods. Modified by the materials with low surface free energy, the composite surface exhibits a robust superhydrophobicity at not only room temperature but also low temperature. After freezing test, the surface can recover to its original function quickly, which has achieved the level for industrial application, and also performed significant role for enhancing the icephobic theories.
A novel flexible micro-ratchet/ZnO nano-rods surface with rapid recovery icephobic performance
Wang, Manxiang,Yu, Wenbo,Zhang, Yupei,Woo, Jae-Young,Chen, Yu,Wang, Bo,Yun, Yanbin,Liu, Guicheng,Lee, Joong Kee,Wang, Lei Elsevier 2018 Journal of industrial and engineering chemistry Vol.62 No.-
<P><B>Abstract</B></P> <P>In this communication, we present a significant method to fabricate a robust icephobic surface on a flexible polymer substrate. The flexible functional surface is obtained by integrating both soft-lithography and crystal growth methods. Modified by the materials with low surface free energy, the composite surface exhibits a robust superhydrophobicity at not only room temperature but also low temperature. After freezing test, the surface can recover to its original function quickly, which has achieved the level for industrial application, and also performed significant role for enhancing the icephobic theories.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ZnO nano-rods are uniformly planted on the flexible PDMS surface. </LI> <LI> Fabrication of the surface integrates both crystal growth and physical vapor deposition methods. </LI> <LI> The superhydrophobic surface successfully realizes fast-recovery from freezing state. </LI> <LI> In freezing process, the nanorods can hardly cracked due to the flexible basement. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>The liquid droplet can recover to sphere state (Cassie's state) after melting from low temperature (for example, 20°C). ▪</P> <P>[DISPLAY OMISSION]</P>
Air Quality Improvement Scenario for China during the 13th Five-Year Plan Period
Qian Tang,Yu Lei,Xiaojun Chen,Wenbo Xue 한국대기환경학회 2017 Asian Journal of Atmospheric Environment (AJAE) Vol.11 No.1
China is suffering from severe air pollution especially fine PM2.5 pollution. In 2015, the annual average PM2.5 concentration of the 338 municipal cities was 50 μg/m3, 78% cities at or above the prefectural level failed to comply with the PM2.5 concentration standards. The 13th Five-Year Plan for National Economic and Social Development set the goal that the annual average concentration of PM2.5 in the municipal cities which failed to attain the ambient air quality standards shall be decreased by 18% by 2020 (CCCPC, 2016). In this study, an air pollution control scenario during the 13th Five-Year Plan period was proposed and the SO2, NOx and PM emission reductions in response to different measures in 31 provincial-level regions mainland China by 2020 were estimated. The air quality in the target year (2020) was simulated using the WRF-CMAQ model. The results showed that by 2020, the emissions of SO2, NOx and primary PM in mainland China will be reduced by 4.19 million tons, 3.94 million tons and 4.41 million tons, a drop of 23%, 21% and 25% respectively compared with that in 2015, and the annual average concentration of PM2.5 will decrease by 19%. Coal-fired power plant contributes the most pollutant emission reduction.
GTP Binding Is Required for SEPT12 to Form Filaments and to Interact with SEPT11
Xiangming Ding,Wenbo Yu,Ming Liu,ShuQing Shen,Fang Chen,Lihuan Cao,Bo Wan,Long Yu 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.3
Septins are a family of filament-forming GTP-binding proteins involved in a variety of cellular process such as cytokinesis, exocytosis, and membrane dynamics. Here we report the biochemical and immunocytochemical characterization of a recently identified mammalian septin, SEPT12. SEPT12 binds GTP in vitro, and a mutation (Gly56 to Asn) in the GTPbinding motif abolished binding. Immunocytochemical analysis revealed that wild-type SEPT12 formed filamentous structures when transiently expressed in Hela cells whereas SEPT12G56A generated large aggregates. In addition, wild-type SEPT12 failed to form filaments when coexpressed with SEPT12G56A. We also observed that GTP-binding by SEPT12 is required for interaction with SEPT11 but not with itself.
Ding, Xiangming,Yu, Wenbo,Liu, Ming,Shen, Suqin,Chen, Fang,Wan, Bo,Yu, Long Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6
Septins are a family of conserved cytoskeletal GTPase forming heteropolymeric filamentous structure in interphase cells, however, the mechanism of assembly are largely unknown. Here we described the characterization of SEPT12, sharing closest homology to SEPT3 and SEPT9. It was revealed that subcelluar localization of SEPT12 varied at interphase and mitotic phase. While SEPT12 formed filamentous structures at interphase, it was localized to the central spindle and to midbody during anaphase and cytokinesis, respectively. In addition, we found that SEPT12 can interact with SEPT6 in vitro and in vivo, and this interaction was independent of the coiled coil domain of SEPT6. Further, co-expression of SEPT12 altered the filamentous structure of SEPT6 in Hela cells. Therefore, our result showed that the interaction between different septins may affect the septin filament structure.