Polymorphisms in TYMS for Prediction of Capecitabine-Induced Hand-Foot Syndrome in Chinese Patients with Colorectal Cancer

Si-Qi Dong,Tong-Min Wang,Jiang-Bo Zhang,Yong-Qiao He,Wen-Qiong Xue,Zi-Yi Wu,Da-Wei Yang,Lian-Jing Cao,Jing-Wen Huang,Xi-Zhao Li,Pei-Fen Zhang,Xiao-Hui Zheng,Wei-Hua Jia 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Purpose Capecitabine is an extensively used oral prodrug of 5-fluorouracil in treatment of colon cancer and is known to cause hand-foot syndrome (HFS). As the target enzyme for capecitabine, thymidylate synthase (TYMS) plays a key role for 5-fluorouracil metabolism and has been associated with some side effects caused by capecitabine. The aim of our study is to identify the possible genetic predictors of capecitabine-induced HFS (CAP-HFS) in Chinese colorectal cancer patients.Materials and Methods Whole exons of TYMS were sequenced for 288 extreme phenotype HFS patients, including 144 severe or early-onset (first 2 cycles) moderate HFS extreme cases and 144 extreme controls with no reported HFS. The associations between polymorphisms and CAP-HFS were analyzed using logistic regression under an additive model.Results We identified a novel risk mutation (c.1A>G, chr18:657743), was associated with severe HFS in an extreme case who was affected during the first cycle of treatment. Moreover, we identified three new variants, rs3786362, rs699517, rs2790, and two previously reported variants, 5’VNTR 2R/3R and 3′-untranslated region 6-bp ins-del, which were significantly associated with CAP-HFS (p < 0.05). In silico analysis revealed that the effect of these polymorphisms in the TYMS region on the development of HFS might not be restricted solely to the regulation of TYMS expression, but also the TYMS catalytic activity through the indirect effect on ENOSF1 expression.Conclusion This study identified new polymorphisms in TYMS gene significantly associated with CAP-HFS, which may serve as useful genetic predictors for CAP-HFS and help to elucidate the underlying mechanism of HFS.

An Improved Active Damping Method with Capacitor Current Feedback

Yi-Wen Geng,Ya-Wen Qi,Hai-Wei Liu,Fei Guo,Peng-Fei Zheng,Yong-Gang Li,Wen-Ming Dong 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.2

Proportional capacitor current feedback active damping (CCFAD) has a limited valid damping region in the discrete time domain as (0, fs/6). However, the resonance frequency (fr) of an LCL-type filter is usually designed to be less than half the sampling frequency (fs) with the symmetry regular sampling method. Therefore, (fs/6, fs/2) becomes an invalid damping region. This paper proposes an improved CCFAD method to extend the valid damping region from (0, fs/6) to (0, fs/2), which covers all of the possible resonance frequencies in the design procedure. The full-valid damping region is obtained and the stability margin of the system is analyzed in the discrete time domain with the Nyquist criterion. Results show that the system can operate stably with the proposed CCFAD method when the resonance frequency is in the region (0, fs/2). The performances at the steady and dynamic state are enhanced by the selected feedback coefficient H and controller gain Kp. Finally, the feasibility and effectiveness of the proposed CCFAD method are verified by simulation and experimental results.

An Improved Active Damping Method with Capacitor Current Feedback

Geng, Yi-Wen,Qi, Ya-Wen,Liu, Hai-Wei,Guo, Fei,Zheng, Peng-Fei,Li, Yong-Gang,Dong, Wen-Ming The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.2

Proportional capacitor current feedback active damping (CCFAD) has a limited valid damping region in the discrete time domain as (0, $f_s/6$. However, the resonance frequency ($f_r$) of an LCL-type filter is usually designed to be less than half the sampling frequency ($f_s$) with the symmetry regular sampling method. Therefore, ($f_s/6$, $f_s/2$) becomes an invalid damping region. This paper proposes an improved CCFAD method to extend the valid damping region from (0, $f_s/6$ to (0, $f_s/2$), which covers all of the possible resonance frequencies in the design procedure. The full-valid damping region is obtained and the stability margin of the system is analyzed in the discrete time domain with the Nyquist criterion. Results show that the system can operate stably with the proposed CCFAD method when the resonance frequency is in the region (0, $f_s/2$). The performances at the steady and dynamic state are enhanced by the selected feedback coefficient H and controller gain $K_p$. Finally, the feasibility and effectiveness of the proposed CCFAD method are verified by simulation and experimental results.

Comparison of the Effects of Propofol and Midazolam on Inflammation and Oxidase Stress in Children with Congenital Heart Disease Undergoing Cardiac Surgery

Wen-fang Xia,Qing-shan Zhou,Yu Liu,Qi-zhu Tang,Han-dong Zou 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.2

Purpose: To investigate and compare the effects of propofol and midazolam on inflammation and oxidase stress in children with congenital heart disease undergoing cardiac surgery. Materials and Methods: Thirty-two ASA class I-II children with congenital heart disease undergoing cardiac surgery were randomly divided into two groups: propofol combined with low dose fentanyl (PF group, n = 16) and midazolam combined with low dose fentanyl (MF group, n = 16). Tracheal extubation time and length of Intensive Care Unit (ICU) stay were recorded. Blood samples were taken before operation (T_0), at 2 h after release of the aorta cross-clamp (T_3) and at 24 h after operation (T_4) to measure interleukin 6 (IL-6), IL-8, superoxide dismutase (SOD) and malondialdehyde (MDA) levels. Myocardium samples were collected at 10-20 min after aorta cross-clamp (T_1) and at 10-20 min after the release of the aorta cross-clamp (T_2) to detect heme oxygenase-1 (HO-1) expression. Results: Tracheal extubation time and length of ICU stay in PF group were significantly shorter than those of the MF group (p < 0.05, respectively). After cardiopulmonary bypass, IL-6, IL-8 and MDA levels were significantly increased, and the SOD level was significantly reduced in both two groups, but PF group exhibited lower IL-6, IL-8 and MDA levels and higher SOD levels than the MF group (p < 0.05, respectively). The HO-1 expression in the PF group was significantly higher than that in MF group at the corresponding time points (p < 0.05, respectively). Conclusion: Propofol is superior to midazolam in reducing inflammation and oxidase stress and in improving post-operation recovery in children with congenital heart disease undergoing cardiac surgery.

Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

Wen Zhong-Ling,Yang Min-Kai,Fazal Aliya,Liao Yong-Hui,Cheng Lin-Run,Hua Xiao-Mei,Hu Dong-Qing,Shi Ji-Sen,Yang Rong-Wu,Lu Gui-Hua,Qi Jin-Liang,Zhi Hong,Qian Qiu-Ping,Yang Yong-Hua 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

Hydrogen-water ameliorates radiation-induced gastrointestinal toxicity via MyD88’s effects on the gut microbiota

Hui-wen Xiao,Yuan Li,Dan Luo,Jia-li Dong,Li-xin Zhou,Shu-yi Zhao,Qi-sheng Zheng,Hai-chao Wang,Ming Cui,Sai-jun Fan 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Although radiation therapy is a cornerstone of modern management of malignancies, various side effects are inevitably linked to abdominal and pelvic cancer after radiotherapy. Radiation-mediated gastrointestinal (GI) toxicity impairs the life quality of cancer survivors and even shortens their lifespan. Hydrogen has been shown to protect against tissue injuries caused by oxidative stress and excessive inflammation, but its effect on radiation-induced intestinal injury was previously unknown. In the present study, we found that oral gavage with hydrogen-water increased the survival rate and body weight of mice exposed to total abdominal irradiation (TAI); oral gavage with hydrogen-water was also associated with an improvement in GI tract function and the epithelial integrity of the small intestine. Mechanistically, microarray analysis revealed that hydrogen-water administration upregulated miR-1968-5p levels, thus resulting in parallel downregulation of MyD88 expression in the small intestine after TAI exposure. Additionally, high-throughput sequencing showed that hydrogen-water oral gavage resulted in retention of the TAI-shifted intestinal bacterial composition in mice. Collectively, our findings suggested that hydrogen-water might be used as a potential therapeutic to alleviate intestinal injury induced by radiotherapy for abdominal and pelvic cancer in preclinical settings.

인공강우기를 이용한 잣나무림의 강우 차단 손실량 추정

이기문 ( Qi Wen Li ),임상준 ( Sang Jun Im ),김동엽 ( Dong Yeob Kim ),이은재 ( Eun Jai Lee ),양현제 ( Hyun Je Yang ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구는 강우 발생 시 수관 및 임상에 의한 차단 손실량을 분석하여 잣나무림의 수문학적특성을 구명하는데 그 목적이 있다. 수관에 의한 차단 손실량을 측정하기 위하여 1.5m × 1.5m 크기의 인공강우기를 이용한 현장실험을 실시하였으며, 인공강우 중 시간에 따른 수관 내 수분 저류량을 Load cell을 이용하여 측정하였다. 실험 결과, 잣나무의 엽면적 당 최대 저류량과 차단 저류량은 각각 127.0g/m2과 38.7g/m2으로 조사되었고, 차단 저류능은 0.14mm∼0.22mm의 범위로 나타났다. 잣나무의 잎과 가지를 순차적으로 제거한 탈엽 실험의 결과, 차단 저류능은 수관 투영면적이 작아짐에 따라 감소하는 경향을 나타났다. 또한, 차단 저류능은 엽면적과도 강한 정의 상관관계를 가진 것으로 판단되었다. 한편, 임상에 의한 차단 손실량은 0.25m × 0.25m 크기의 소형 인공강우기를 이용한 실내실험을 통하여 측정하였다. 실험 결과, 잣나무 낙엽의 최대 저류량은 2.20g/g이고, 차단 저류량은 1.15g/g으로 나타났다. 최종적으로 실험에 이용된 잣나무 잎의 단위면적당 차단 저류량은 88.1g/m2으로 추정되었다. This study was conducted to analyse hydrological characteristics of Pinus Koraiensis forest on calculating interception of canopy and forest floor. For estimating canopy interception loss, a rainfall simulator that could cover 1.5m × 1.5m area was used and a load cell was used for measuring temporal differency of holden water weight on canopy. As a controlled experiment, sample trees were defoliated 2 times. Without defoliation, the result of interception experiment indicates that maximum water detention and interception loss per leaf surface area were 127.0g/m2 and 38.7g/m2 respectively. Furthermore, interception storage capacity of sample was supposed as 0.14∼0.22mm. In addition, defoliated experiment showed relationship between canopy interception and projected area, however, the correlation of interception with leaf surface area was higher. On the other hand, to estimate forest floor interception loss, a smaller rainfall simulator beyond an area of 0.25m × 0.25m was used indoors and an electronic scale recorded temporal differency of water on litter layer. The maximum water detention and interception loss of litter layer are 2.20g/g and 1.15g/g respectively. The result of interception loss per litter surface area was 88.1g/m2.

Test on the anchoring components of steel shear keys in precast shear walls

Shao-Dong Shen,Peng Pan,Wen-Feng Li,Qi-Song Miao,Run-Hua Gong 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.24 No.6

Prefabricated reinforced-concrete shear walls are used extensively in building structures because they are convenient to construct and environmentally sustainable. To make large walls easier to transport, they are divided into smaller segments and then assembled at the construction site using a variety of connection methods. The present paper proposes a precast shear wall assembled using steel shear keys, wherein the shear keys are fixed on the embedded steel plates of adjacent wall segments by combined plug and fillet welding. The anchoring strength of shear keys is known to affect the mechanical properties of the wall segments. Loading tests were therefore performed to observe the behavior of precast shear wall specimens with different anchoring components for shear keys. The specimen with insufficient strength of anchoring components was found to have reduced stiffness and lateral resistance. Conversely, an extremely high anchoring strength led to a short-column effect at the base of the wall segments and low deformation ability. Finally, for practical engineering purposes, a design approach involving the safety coefficient of anchoring components for steel shear keys is suggested.

Comparative Analysis of Outcomes and Clinicopathological Characteristics of Synchronous and Metachronous Contralateral Breast Cancer: A Study of the SEER Database

Ruiyue Qiu,Wen Zhao,Jiao Yang,Yanwei Shen,Biyuan Wang,Pan Li,Andi Zhao,Qi Tian,Mi Zhang,Min Yi,Jin Yang,Danfeng Dong 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Numerous previous studies have reported inconsistent results about the differences between synchronous contralateral breast cancer (sCBC) and metachronous contralateral breast cancer (mCBC). This study aimed to compare the clinical characteristics and outcomes between sCBC and mCBC and determine predictive factors for the survival of sCBC and mCBC patients. Methods: Using the Surveillance, Epidemiology, and End Results Program database, we identified sCBC or mCBC patients from 2000 to 2010. The Kaplan-Meier method and Cox proportional hazards regression analysis were used to analyze overall survival and breast cancer-specific survival (BCSS) rates of sCBCs and mCBCs, respectively. Results: Overall, 14,057 sCBC (n = 8,139, 57.9%) and mCBC (n = 5,918, 42.1%) patients were included. The first tumors of sCBC were more likely to have higher stage and more lymph and distant metastases, whereas those of mCBC were more often infiltrating ductal carcinoma (IDC), had localized stage, were estrogen receptor (ER) and progesterone receptor (PR) negative, and had less axillary nodal involvement. The second tumors of mCBC tended to be IDC and have higher grade, adverse stage, ER and PR-negativity; and more axillary nodal involvement, compared to the second tumors of sCBC. mCBC patients had significantly favorable 5-year BCSS but worse long-term BCSS compared with sCBC patients. Moreover, subgroup analysis revealed no significant difference of BCSS between sCBC and mCBC among patients aged 18–60 years. Multivariate analysis indicated that age, grade, and stage of 2 tumors; surgery for second tumor; and ER status of the second tumor were independent prognostic factors for BCSS of contralateral breast cancer (CBC). Conclusion: The characteristics and outcomes of sCBCs and mCBCs were substantially different. sCBC and mCBC patients may have different prognosis, and the prognosis of CBC depends on the first and second tumors.

일본 애니메이션에 나타난 언령신앙(言霊信仰)에 관한 연구 - TV 애니메이션 〈나츠메 우인장(夏目友人帳)〉을 중심으로

서치린(She, Qi-Lin),최동혁(Choi, Dong-Hyuk),자오원장(Zhao, Wen-Jiang) 한국만화애니메이션학회 2021 만화애니메이션연구 Vol.- No.62

고대의 일본 사람은 세상 만물에 신령(神靈)이 존재하며, 특히 언어(言語)에서의 신령이 가지는 영력(靈力)이 사람들에게 복(福)이나 재(災)를 가져다줄 수 있다고 주장하였다. 이러한 언어에 대한 숭배는 언령신앙(言霊信仰)이라고 부른다. 상대(上代)문학의 많은 작품들이 언령신앙을 구현했거나 부응시켰는데, 이는 상대(上代)문학의 중요한 특징 중 하나가 되었다. 그러나 언령신앙에 관한 기존 연구들은 주로 초기의 문학 형식이나 문학 작품을 고찰에 그치고 있으며, 사회의 발전에 따라 언령신앙이 현대사회에서 어떻게 계속되고 구현되는지에 대한 연구는 부족하였다. 이에 본 연구는 상대(上代)문학에서 언령신앙의 특징을 기반으로 기존의 선행연구들을 정리함으로써 언령신앙과 애니메이션의 결합 가능성을 탐구하고자 하였다. 즉, 애니메이션 작품에서 언령신앙의 출현배경과 표현사례를 검토하여 ‘캐릭터의 표현’, ‘이름(名前)의 활용’과 ‘주술(呪術)과 마법의 활용’ 등 세 가지의 측면에서 TV 애니메이션 <나츠메 우인장(夏目友人帳)>에서 나타난 언령신앙의 구체적인 표현을 분석하였다. 이를 통해 애니메이션과 같은 현대 예술작품에서 언령신앙이 구현되는 구체적인 표현방법과 창작 방법을 도출하였다. 언령신앙과 같은 고대의 일본 문화나 사상을 소재로 한 애니메이션의 대표적인 표현사례를 분석한 본 연구가 다양한 고대사상과 전통문화를 이해를 돕고 많은 문화콘텐츠 분야에 활용과 발전에 기여되기를 기대한다. The ancient Japanese believed that there are gods in everything in the world. Souls and spiritual powers in language, which can bring blessings or disasters to people. This kind of worship of language is called language worship. In the early Japanese literature, there are many examples of language worship, which has become one of the important characteristics of early Japanese literature. However, the existing research on language worship mainly stays on the investigation of early literary forms or works. With the development of society, there is insufficient research on how the language worship continues and manifests in modern society. This research is based on the language worship in early Japanese literature, investigating the existing advanced research, and exploring the possibility of combining language worship with animation. Language worship is mainly manifested in three types in animation: ‘character performance’, ‘utilization of names’, and ‘utilization of spells and magic.’ This research analyzes the background and examples of the appearance of language worship in animation and analyzes language worship in the TV animation 〈Natsume"s Book of Friends〉. Through these, we learned about the examples and methods of ancient thoughts and traditional culture used in animation and other works of modern art. This research uses ancient Japanese culture or ideas such as language worship as materials to analyze representative examples of animation. It is hoped that this research can help to understand various ancient ideas and traditional cultures, and contribute to the utilization and development of cultural fields.