Listeria monocytogenes Serovar 4a is a Possible Evolutionary Intermediate Between L. monocytogenes Serovars 1/2a and 4b and L. innocua

Jian Shun Chen,Ling Li Jiang,Xue Yan Chen,Xiao Kai Luo,Yang Chen,Ying Yu,Guo Ming Tian,Dong You Liu,Wei Huan Fang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.3

Characterization of Surface Layer Proteins in Lactobacillus crispatus Isolate ZJ001

Xue Yan Chen,Yang Chen,Xiao Liang Li,Ning Chen,Wei Huan Fang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.10

Experimental investigation of crack growth behaviors and mechanical properties degradation during gear bending fatigue

Huan Yan,Peitang Wei,Pengliang Zhou,Ling Chen,Huaiju Liu,Caichao Zhu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.3

Tooth bending fatigue remains as a fundamental bottleneck restricting the safety and reliability of modern high-performance gear. In this study, a serial of bending fatigue tests on case carburized and shot peening treated gears is conducted. The fatigue crack propagation behaviors during bending fatigue test were analyzed. The alternations of macroscopic mechanical properties and microstructure features of gear samples subjected to bending fatigue are experimentally examined and analyzed in detail. It is found that the measured tooth root crack trajectories basically conform to the dangerous section of the tooth root determined by the 30° tangent method with a slight variation of about 1°. The phenomena of residual stress relaxation in gear bending fatigue is observed with the maximum residual compressive stress decreasing from -698 MPa of intact state to -572 MPa of failure state. Furthermore, the content of retained austenite of tooth root surface decreases by about 3.8 % after the gear bending fatigue failure, and the grain size of the tooth root core region illustrates a certain tendency of coarsening.

Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks

Wei Huan,Liu Jiancheng,Liu Mengjian,Zhang Huiling,Chen Yong 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

Objective: This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. Methods: Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. Results: Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. Conclusion: Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.

Dynamic response analysis for structures with interval parameters

Chen, Su Huan,Lian, Hua Dong,Yang, Xiao Wei Techno-Press 2002 Structural Engineering and Mechanics, An Int'l Jou Vol.13 No.3

In this paper, a new method to solve the dynamic response problem for structures with interval parameters is presented. It is difficult to obtain all possible solutions with sharp bounds even an optimum scheme is adopted when there are many interval structural parameters. With the interval algorithm, the expressions of the interval stiffness matrix, damping matrix and mass matrices are developed. Based on the matrix perturbation theory and interval extension of function, the upper and lower bounds of dynamic response are obtained, while the sharp bounds are guaranteed by the interval operations. A numerical example, dynamic response analysis of a box cantilever beam, is given to illustrate the validity of the present method.

Liquiritin inhibits TGF-β1-induced epithelial mesenchymal transition and extracellular matrix deposition in human renal proximal tubular epithelial cells by suppressing the MAPK signaling

Chen Zhen,Liu Yi jue,Yu Bo,Li Wei,Zhang Mengli,Wu Xian,Gui Feng,Peng Huan,Ai Fen 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.3

Background Excessive extracellular matrix (ECM) deposition leads to renal fibrosis, a typical hallmark of chronic kidney disease. Liquiritin is a flavonoid extracted from the rhizome part of Glycyrrhiza glabra and has anti-fibrotic and nephroprotective effects. However, its role and underlying mechanism in renal fibrosis remain unknown. Methods Human renal proximal tubular epithelial cells (HRPTEpiCs) were stimulated with 10 ng/mL TGF-β1 to induce renal fibrosis models in vitro. The morphology of HRPTEpiCs was observed under a light microscope. CCK-8 was utilized to test cell viability. Immunofluorescence staining was conducted to measure α-SMA expression in HRPTEpiCs. RT-qPCR was used to assess relative mRNA expression. The protein levels of ECM markers, epithelial mesenchymal transition (EMT) markers, and MAPK signaling-related molecules in HRPTEpiCs were tested using western blotting. Results TGF-β1-treated HRPTEpiCs showed a fibroblast-like morphology, and the morphology of HRPTEpiCs was restored by liquiritin. Liquiritin suppressed TGF-β1-stimulated ECM deposition and EMT process in HRPTEpiCs. Additionally, liquiritin repressed TGF-β1-induced MAPK signaling activation in HRPTEpiCs. Conclusion Liquiritin mitigates TGF-β1-triggered EMT process and ECM deposition in HRPTEpiCs by inactivating MAPK signaling, thus preventing renal fibrosis. Background Excessive extracellular matrix (ECM) deposition leads to renal fibrosis, a typical hallmark of chronic kidney disease. Liquiritin is a flavonoid extracted from the rhizome part of Glycyrrhiza glabra and has anti-fibrotic and nephroprotective effects. However, its role and underlying mechanism in renal fibrosis remain unknown. Methods Human renal proximal tubular epithelial cells (HRPTEpiCs) were stimulated with 10 ng/mL TGF-β1 to induce renal fibrosis models in vitro. The morphology of HRPTEpiCs was observed under a light microscope. CCK-8 was utilized to test cell viability. Immunofluorescence staining was conducted to measure α-SMA expression in HRPTEpiCs. RT-qPCR was used to assess relative mRNA expression. The protein levels of ECM markers, epithelial mesenchymal transition (EMT) markers, and MAPK signaling-related molecules in HRPTEpiCs were tested using western blotting. Results TGF-β1-treated HRPTEpiCs showed a fibroblast-like morphology, and the morphology of HRPTEpiCs was restored by liquiritin. Liquiritin suppressed TGF-β1-stimulated ECM deposition and EMT process in HRPTEpiCs. Additionally, liquiritin repressed TGF-β1-induced MAPK signaling activation in HRPTEpiCs. Conclusion Liquiritin mitigates TGF-β1-triggered EMT process and ECM deposition in HRPTEpiCs by inactivating MAPK signaling, thus preventing renal fibrosis.

Uniaxial Compression Properties and Compression Fatigue Performance of Selective Laser Melted Ti–6Al–4V Cellular Structure

Ming‑Wei Wu,Jhewn‑Kuang Chen,Mo‑Kai Tsai,Pei Wang,Tien‑Lin Cheng,Bo‑Huan Lin,Po‑Hsing Chiang,Annadurai Dhinakar 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1

The selective laser melted (SLM) Ti–6Al–4V cellular solid is a promising material in biomedical and other applications. The mechanical properties of the SLM Ti–6Al–4V cellular material are predominantly controlled by the design of the cellular structure. The objective of this study was to investigate the uniaxial compressive properties and compressive fatigueperformance of the SLM Ti–6Al–4V cellular solid with a new cuboctahedron unit cell reinforced by four diagonal strutsand two horizontal struts along the X and Y axes. The infuences of hot isostatic pressing (HIP) on the various mechanicalproperties were also examined. The results showed a smoother stress–strain curve and fewer stress oscillations in the HIPspecimen than in the SLM specimen. The HIP process slightly decreased the yield strength of the SLM sample from 55 to52 MPa. Under uniaxial compressive stress, the SLM and HIP specimens exhibited layer-by-layer fracture and diagonalshear fracture, respectively. Furthermore, the HIP treatment signifcantly increased the fatigue performance and improved thefatigue endurance ratio at 106cycles from 0.15 to 0.35. The HIP treatment contributed to several positive efects, includingphase transformation from brittle α′ to α + β, the elimination of strut porosity and residual stress, an increase in lath size,and a better combination of strength and ductility, thereby improving the fatigue performance. The efects of stress mode onthe fracture mechanism were also clarifed using in situ observation in combination with digital image correlation.

Reactive Power-Voltage Integrated Control Method Based on MCR

Dan Chen,Xiaosheng Huang,Guangsheng Li,Yi Wei,Wenjun Zeng,Chuihua Tian,Huan Shi,Hua Ye 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

In power system, the voltage pulsation and deficiency or surplus of reactive power will have a great effect on the quality of electric power supply. Dynamicly local reactive power compensation is one of the best methods to reduce the power network loss and improve the efficiency of transmission and distribution.Through the effective methods of compensation, we can keep the balance of reactive power in the maximum degree by the maintenance of acceptable voltage. This paper firstly describes the principles of synthetic compensation of reactive power and voltage then analyzes the principle of magnetically controlled reactor-MCR in detail and derives the characteristics, secondly analyses the disadvantages of the conventional reactive power compensation devices and the advantages of MCR, thirdly on this basis designs a control strategy of reactive power and voltage which is based on MCR,so as to achieve the goal of dynamic reactive power compensation. At the end of paper, simulator and experimental research are carried out for the model of compensation method; the results accord with the theoretical analyses done before. So the compensation method is proved to be accurate in theory and feasible in practice.

Genetic Organization of ascB-dapE Internalin Cluster Serves as a Potential Marker for Listeria monocytogenes Sublineages IIA, IIB, and IIC

( Jian Shun Chen ),( Chun Fang ),( Ning Yu Zhu ),( Yong Hui Lv ),( Chang Yong Cheng ),( Yi Jiang Bei ),( Tianlun Zheng ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5

Listeria monocytogenes is an important foodborne pathogen that comprises four genetic lineages: I, II, III, and IV. Of these, lineage II is frequently recovered from foods and environments and responsible for the increasing incidence of human listeriosis. In this study, the phylogenetic structure of lineage II was determined through sequencing analysis of the ascB-dapE internalin cluster. Fifteen sequence types proposed by multilocus sequence typing based on nine housekeeping genes were grouped into three distinct sublineages, IIA, IIB, and IIC. Organization of the ascBdapE internalin cluster could serve as a molecular marker for these sublineages, with inlGHE, inlGC2DE, and inlC2DE for IIA, IIB, and IIC, respectively. These sublineages displayed specific genetic and phenotypic characteristics. IIA and IIC showed a higher frequency of recombination (ρ/θ). However, recombination events had greater effect (r/m) on IIB, leading to its high nucleotide diversity. Moreover, IIA and IIB harbored a wider range of internalin and stress-response genes, and possessed higher nisin tolerance, whereas IIC contained the largest portion of low-virulent strains owing to premature stop codons in inlA. The results of this study indicate that IIA, IIB, and IIC might occupy different ecological niches, and IIB might have a better adaptation to a broad range of environmental niches.

AEG-1 aggravates inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions

Juan Zhao,Wei Ma,Weizhi Chen,Jie Gao,Chunling Li,Yahong Tong,Qin Zhou,Xiuling Zhao,Menghua Wang,Huan Xiao,Yanrong Jin 한국통합생물학회 2019 Animal cells and systems Vol.23 No.6

Endometriosis (EMs) is one of the most common gynaecological diseases in women of childbearing age. Astrocyte elevated gene-1 (AEG-1) is associated with the invasion, migration, apoptosis and prognosis of various cancers. However, the roles of AEG-1 in EMs and its corresponding molecular mechanism are still unknown. In this study, animal models of EMs were established and mice were divided into two groups (n = 10): Sham group and EMs group. The EMs cells were isolated from EMs model. The AEG-1 gene was knocked down by shRNA, while the SOCS1 gene was knocked down by siRNA. Histological changes, AEG-1 expression in tissues and inflammatory factors level were detected by H&E staining, immunohistochemistry and ELISA, respectively. RTqPCR and western blotting were used to determine the expression level of related proteins. The present study found AEG-1 was up-regulated in the EMs model. Enhanced AEG-1 promoted inflammatory cell infiltration, and elevated the levels of IL-1β, IL-6, and TNF-α in EM group (p < 0.05). Besides, AEG-1 overexpression promoted the expression of NALP3, ASC and Cleavedcaspase- 1, while decreased SOCS1 level (p < 0.05). Decrease of SOCS1 further promoted the formation of NALP3 inflammasome. The inhibitory effect of AEG-1 on SOCS1 was weakened after the addition of MG-132 (p < 0.01). Furthermore, silencing AEG-1 alone increased SOCS1 level, decreased the levels of inflammatory cytokines, thereby inhibited the formation of NALP3 inflammasome. All these results demonstrated that AEG-1 aggravated inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions.