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( Jian Shun Chen ),( Chun Fang ),( Ning Yu Zhu ),( Yong Hui Lv ),( Chang Yong Cheng ),( Yi Jiang Bei ),( Tianlun Zheng ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5
Listeria monocytogenes is an important foodborne pathogen that comprises four genetic lineages: I, II, III, and IV. Of these, lineage II is frequently recovered from foods and environments and responsible for the increasing incidence of human listeriosis. In this study, the phylogenetic structure of lineage II was determined through sequencing analysis of the ascB-dapE internalin cluster. Fifteen sequence types proposed by multilocus sequence typing based on nine housekeeping genes were grouped into three distinct sublineages, IIA, IIB, and IIC. Organization of the ascBdapE internalin cluster could serve as a molecular marker for these sublineages, with inlGHE, inlGC2DE, and inlC2DE for IIA, IIB, and IIC, respectively. These sublineages displayed specific genetic and phenotypic characteristics. IIA and IIC showed a higher frequency of recombination (ρ/θ). However, recombination events had greater effect (r/m) on IIB, leading to its high nucleotide diversity. Moreover, IIA and IIB harbored a wider range of internalin and stress-response genes, and possessed higher nisin tolerance, whereas IIC contained the largest portion of low-virulent strains owing to premature stop codons in inlA. The results of this study indicate that IIA, IIB, and IIC might occupy different ecological niches, and IIB might have a better adaptation to a broad range of environmental niches.
Effect of Heat Treatment on the Lipophillic Pigments of Fresh Green Tea Liquor
Jian-Liang Lu,Zhan-Bo Dong,Shun-Shun Pan,Chen Lin,Xin-Qiang Zheng,Borthakur Devajit,Yue-Rong Liang 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3
Changes in lipophillic pigments concentration and its relation to color of fresh green tea liquor during heat treatment were studied. The results showed liquor greenness decreased markedly with extension of incubation time at 55℃, while the brightness and yellowness changed a little. Significant increase in ‘a’ and ‘b’ values of tea liquor was observed at 95℃. Color change of liquor at 55℃ was accompanied by a decrease in the level of chlorophylls, lutein and neoxanthin, and an increase in the pheophytins and β-carotene levels. However, all pigments except β-carotene decreased with time extension at 95℃. Significant correlation was found between pigments and color difference index. The browning of fresh green tea liquor was attributed to vicissitudes of lipophillic pigments during heat treatment, especially to the change of chlorophylls/pheophytins ratio. Result also showed addition of Zn²? at 1.6 μ㏖/ℓ could partially alleviate the decrease in greenness during heat treatment.
Jian-Fang Liu,Xu-Guang Sun,Xiaoyang Jiao,Hong-Xia Chen,Shun-Ming Hua,Hong-Chun Zhang 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.2
To levitate ICF target spheres in the near-field acoustic levitation, a transducer with concave spherical radiating surface and a near-field acoustic levitation system is established. The concave spherical radiating surface of the transducer is designed by the finite element parametric method. Then the levitation height and levitation perturbation of spheres with different mass and diameters in the near-field acoustic levitation system are tested and discussed in the driving voltage at 400V, 500V and 600V, respectively, when the levitation system is under the resonant frequency. Finally, based on the experimental results, the height formula of the near-field acoustic levitation for spheres is deduced by introducing a coupling coefficient.
Jian-Shian Lin,Ming-Hua Chung,Chen-Ming Chen,Fuh-Shyang Juang,Yu-Sheng Tsai,Shu-Wei Chang,Shun-Hsi Wang,Teh-Chao Liao,Lung-Chang Liu 한국물리학회 2010 Current Applied Physics Vol.10 No.5
UV-curable organic/inorganic hybrid composites with gas barrier and heat-dissipating capability have been successfully fast synthesized with microwave irradiation and utilized for the encapsulation of top-emitting organic light-emitting diodes (TEOLEDs). Experimental results manifest that lab-made organic/inorganic hybrid composites can effectively not only obstruct the invasion of moisture as well as oxygen in the atmosphere into the device but also lower the temperature of device. Therefore, the lifetimes of TEOLEDs with their encapsulation are 2.2 folds longer than those without encapsulation.
Jiang, Jian-Tao,Zhang, Lan-Fang,Zhou, Bin,Zhang, Shun-Qun,Li, Shao-Min,Zhang, Wei,Zhang, Jin,Qiao, Zhe,Kong, Ran-Ran,Ma, Yue-Feng,Chen, Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
Objective: To investigate uPA and VEGF expression in esophageal cancer and relations with tumorous invasion and metastasis. Methods: Immunohistochemistry was used to detect uPA and VEGF expression in the normal epithelial tissue of esophageal mucosa and cancer tissue and detect CD34 labeled micrangium and analyze the relationships with clinical pathological features and tumor angiogenesis. Results: Positive rates for uPA and VEGF protein expression were significantly greater in esophageal cancer than normal epithelial tissue (P < 0.05), the two being linked (P <0.05). In addition, uPA and VEGF protein expression of the high microvessel density (MVD) group was significantly lower than in the low MVD group (P < 0.05), with relation to clinical pathological staging, differentiation and lymph node metastasis (P < 0.05). Conclusion: In esophageal cancer tissue, uPA and VEGF proteins are overexpressed and promote tumor angiogenesis, indicative of a poor prognosis.
Li, Jian,Xu, Ya-Hong,Lu, Yi,Ma, Xiao-Ping,Chen, Ping,Luo, Shun-Wen,Jia, Zhi-Gang,Liu, Yang,Guo, Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9
Purpose: Prostate cancer caused by the abnormal disorderly growth of prostatic acinar cells is the most prevalent cancer of men in western countries. We aimed to screen out differentially expressed genes (DEGs) and explore small molecule drugs for prostate cancer. Materials and Methods: The GSE3824 gene expression profile of prostate cancer was downloaded from Gene Expression Omnibus database which including 21 normal samples and 18 prostate cancer cells. The DEGs were identified by Limma package in R language and gene ontology and pathway enrichment analyses were performed. In addition, potential regulatory microRNAs and the target sites of the transcription factors were screened out based on the molecular signature database. In addition, the DEGs were mapped to the connectivity map database to identify potential small molecule drugs. Results: A total of 6,588 genes were filtered as DEGs between normal and prostate cancer samples. Examples such as ITGB6, ITGB3, ITGAV and ITGA2 may induce prostate cancer through actions on the focal adhesion pathway. Furthermore, the transcription factor, SP1, and its target genes ARHGAP26 and USF1 were identified. The most significant microRNA, MIR-506, was screened and found to regulate genes including ITGB1 and ITGB3. Additionally, small molecules MS-275, 8-azaguanine and pyrvinium were discovered to have the potential to repair the disordered metabolic pathways, abd furthermore to remedy prostate cancer. Conclusions: The results of our analysis bear on the mechanism of prostate cancer and allow screening for small molecular drugs for this cancer. The findings have the potential for future use in the clinic for treatment of prostate cancer.
Cai-Yi Wen,Shun-Shan Shen,Zhi-Gang Yin,Kai-Xuan Wang,Jian-Guang Chen 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.4
Bacillus subtilis EBS05, an endophytic bacteria strain isolated from a medicinal plant Cinnamomum camphor,can produce antagonistic compounds that effectively inhibit plant pathogenic fungi. The greenhouse experiments showed that wheat sharp eyespot disease (WSED)was reduced by 91.2%, 88.2% and 43.0% after the treatment with fermentation broth, bacteria-free filter and a fungicide fludioxonil, respectively. The culture broth of strain EBS05 can more effectively control WSED than can fludioxonil. The fermentation broth and bacteria-free filter ability to suppress WSED was not significantly different, suggesting that an active secreted substance played a major role in controlling WSED. Separation and purification of the active compounds was carried out by serial processes, including hydrochloric acid (pH 2.0) treatment, methanol extraction and Sephadex LH-20 column chromatography,silica gel column chromatography and reverse-phase high-pressure liquid chromatography (HPLC), respectively. The purified compounds, one of active peaks in the HPLC spectrum, were obtained from the collection. Analysis of the chemical structures by time-of-flight mass spectrometry (TOF-MS) and electrospray ionization mass spectrometry/mass spectrometry (ESI-MS/MS) showed that the active substances produced by the endophytic bacteria EBS05 are mixture of the β-hydroxy-C12~C15-Leu^7 surfactin A isomers with 1035.65Da, 1021.64 Da, 1007.63 Da and 993.65 Da molecular weights, respectively.
Geographic Distribution and Epidemiology of Lung Cancer During 2011 in Zhejiang Province of China
Lin, Xia-Lu,Chen, Yan,Gong, Wei-Wei,Wu, Zhao-Fan,Zou, Bao-Bo,Zhao, Jin-Shun,Gu, Hua,Jiang, Jian-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
Background: To explore etiology for providing scientific clues for the prevention of lung cancer. Materials and Methods: Data for lung cancer incidence and meteorological geographic factors from 25 counties in Zhejiang province of China during 2011 were studied. Stepwise multiple regression and correlation analysis were performed to analyze the geographic distribution and epidemiology of lung cancer. Results: 8,291 new cases (5,998 in males and 2,293 females) of lung cancer during 2011 in Zhejiang province were reported in the 25 studied counties. Reported and standardized incidence rates for lung cancer were 58.0 and 47.0 per 100,000 population, respectively. The incidence of lung cancer increased with age. Geographic distribution analysis shows that the standardized incidence rates of lung cancer in northeastern Zhejiang province were higher than in the southwestern part, such as in Nanhu, Fuyang, Wuxing and Yuyao counties, where the rates were more than 50 per 100,000 population. In the southwestern Zhejiang province, for instance, in Yueqing, Xianju and Jiande counties, the standardized incidence rates of lung cancer were lower than 37 per 100,000 population. Spearman correlation tests showed that forest coverage rate, air quality index (AQI), and annual precipitation level are associated with the incidence of lung cancer. Conclusions: Lung cancer in Zhejiang province shows obvious regional differences. High incidence appears associated with low forest coverage rate, poor air quality and low annual precipitation. Therefore, increasing the forest coverage rate and controlling air pollution may play an important role in lung cancer prevention.