The Molecular Mechanism of Long Non-Coding RNA MALAT1-Mediated Regulation of Chondrocyte Pyroptosis in Ankylosing Spondylitis

Wei Chen,Feilong Wang,Jiangtao Wang1,Fuyu Chen,Ting Chen 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.6

Long non-coding RNAs (lncRNAs) may be important regulators in the progression of ankylosing spondylitis (AS). The competing endogenous RNA (ceRNA) activity of lncRNAs plays crucial roles in osteogenesis. We identified the mechanism of the differentially expressed lncRNA MALAT1 in AS using bioinformatic analysis and its ceRNA mechanism. The interaction of MALAT1, microRNA-558, and GSDMD was identified using integrated bioinformatics analysis and validated. Loss- and gain-of-function assays evaluated their effects on the viability, apoptosis, pyroptosis and inflammation of chondrocytes in AS. We found elevated MALAT1 and GSDMD but reduced miR-558 in AS cartilage tissues and chondrocytes. MALAT1 contributed to the suppression of cell viability and facilitated apoptosis and pyroptosis in AS chondrocytes. GSDMD was a potential target gene of miR-558. Depletion of MALAT1 expression elevated miR-558 by inhibiting GSDMD to enhance cell viability and inhibit inflammation, apoptosis and pyroptosis of chondrocytes in AS. In summary, our key findings demonstrated that knockdown of MALAT1 served as a potential suppressor of AS by upregulating miR-558 via the downregulation of GSDMD expression.

Mental Health Problems of Individuals Under the Stay-Home Policy

Wang Hayson Chenyu,Ting Wenyun,Li Zhijin,Sun Elliot Tianyu,Wang Xiaojun 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.7

Mitigating SYN flooding Attack and ARP Spoofing in SDN Data Plane

Ting-Yu Lin,Jhen-Ping Wu,Pei-Hsuan Hung,Ching-Hsuan Shao,Yu-Ting Wang,Yun-Zhan Cai,Meng-Hsun Tsai 한국통신학회 2020 한국통신학회 APNOMS Vol.2020 No.09

As the number of network devices increases rapidly, it becomes more and more difficult to defend network attacks. Large-scaled attacks, such as SYN flooding, may lead to heavy burden to the switches as well as the controller in a software defined network (SDN). In this paper, we investigate the SYN flooding and Address Resolution Protocol (ARP) spoofing attacks in SDN, and then propose mechanisms to address these two attacks. We also present a new scheme to detect SYN flooding by using only a few forwarding rules. Moreover, we utilize the Programming Protocol-independent Packet Processors (P4) technique to mitigate the burden of the controller.

Recent progress of continuous crystallization

Ting Wang,Haijiao Lu,Jingkang Wang,Yan Xiao,Yanan Zhou,Ying Bao,Hongxun Hao 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-

Continuous crystallization has always been a hot topic in industrial crystallization. Many efforts have been made to improve the continuous crystallization, either by designing novel continuous crystallizers or by proposing improved design and operation of conventional continuous crystallizers. Some new models for continuous crystallization processes have also been proposed and tested in recent years. In this work, the development of continuous crystallization in recent years, including novel crystallizers, control strategies, models and some assistive technologies, is summarized. Promising as it is, continuous crystallization is still not as universal as batch crystallization due to the existence of the drawbacks, such as blockage and encrustation. Therefore, further efforts are needed before wider application of continuous crystallization.

Taxol Produced from Endophytic Fungi Induces Apoptosis in Human Breast, Cervical and Ovarian Cancer Cells

Wang, Xin,Wang, Chao,Sun, Yu-Ting,Sun, Chuan-Zhen,Zhang, Yue,Wang, Xiao-Hua,Zhao, Kai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Currently, taxol is mainly extracted from the bark of yews; however, this method can not meet its increasing demand on the market because yews grow very slowly and are a rare and endangered species belonging to first-level conservation plants. Recently, increasing efforts have been made to develop alternative means of taxol production; microbe fermentation would be a very promising method to increase the production scale of taxol. To determine the activities of the taxol extracted from endophytic fungus N. sylviforme HDFS4-26 in inhibiting the growth and causing the apoptosis of cancer cells, on comparison with the taxol extracted from the bark of yew, we used cellular morphology, cell counting kit (CCK-8) assay, staining (HO33258/PI and Giemsa), DNA agarose gel electrophoresis and flow cytometry (FCM) analyses to determine the apoptosis status of breast cancer MCF-7 cells, cervical cancer HeLa cells and ovarian cancer HO8910 cells. Our results showed that the fungal taxol inhibited the growth of MCF-7, HeLa and HO8910 cells in a dose-and time-dependent manner. IC50 values of fungal taxol for HeLa, MCF-7 and HO8910 cells were $0.1-1.0{\mu}g/ml$, $0.001-0.01{\mu}g/ml$ and $0.01-0.1{\mu}g/ml$, respectively. The fungal taxol induced these tumor cells to undergo apoptosis with typical apoptotic characteristics, including morphological changes for chromatin condensation, chromatin crescent formation, nucleus fragmentation, apoptotic body formation and G2/M cell cycle arrest. The fungal taxol at the $0.01-1.0{\mu}g/ml$ had significant effects of inducing apoptosis between 24-48 h, which was the same as that of taxol extracted from yews. This study offers important information and a new resource for the production of an important anticancer drug by endofungus fermentation.

Evaluation of secondary cytoreduction surgery in platinum-resistant ovarian cancer patients within three-line recurrent: a multicenter, randomized controlled study

Ting-Ting Chen,Junfen Xu,Bairong Xia,Hui Wang,Yuanming Shen 대한부인종양학회 2024 Journal of Gynecologic Oncology Vol.35 No.1

Background: Epithelial ovarian cancer is the leading cause of death among gynecologicalmalignancies. Platinum resistance remains a dilemma and bottleneck in treatment, andsalvage chemotherapy has limited effectiveness. Recently, the role of secondar y cytoreductivesurger y (SCS) in patients with platinum-resistant recurrent ovarian cancer (ROC) has causedattention especially in patients with oligometastases. However, there is neither high-qualityevidence-based evidence nor standardized criteria for selecting SCS for patients withplatinum-resistant ROC until now. Methods: This multicenter, randomized, controlled clinical trial is to evaluate the valueof SCS and to clarif y reliable criteria of utilizing SCS in women with ROC, which is led byGynecologic Oncology Group, Women’s Hospital, Zhejiang University School of Medicine. Recruitment has started on Januar y 1st, 2023, and is scheduled to end in December 2026. One hundred and forty participants with platinum-resistant ROC who meet the “RSCScriteria” will be randomized assigned at a ratio of 1:1 to either the experimental arm or thestandard arm. Patients in the experimental arm will receive SCS followed by non-platinumsingle agent chemotherapy (paclitaxel, gemcitabine or liposomal adriamycin) for at least4 cycles while patients in the standard arm will be provided with only non-platinum singleagent chemotherapy. The primar y outcome is progression-free sur vival. The secondar youtcomes are overall sur vival, adverse events and health-related cancer-specific quality of life. Trial Registration: ClinicalTrials.gov Identifier: NCT05633199

Inhibition of Proliferation and Induction of Apoptosis by the Combination of β-carotene and 1,25-dihydroxyvitamin D3 in Human Esophageal Cancer EC9706 Cells

Wang, Shao-Kang,Yang, Lei,Wang, Ting-Ting,Huang, Gui-Ling,Yang, Li-Gang,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Esophageal cancer is a common malignant tumor occurring in human esophageal epithelial tissue. The primary purpose of this paper was to define the effects of ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$, alone and in combination, on cell proliferation, cell cycle and apoptosis of human esophageal cancer EC9706 cells. Treatment with different concentrations of ${\beta}$-carotene and/or 1,25-dihydroxyvitamin $D_3$. MTT assay showed that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ significantly inhibited proliferation of EC9706 cells in a dose- and time-dependent manner. Further studies also demonstrated that ${\beta}$-carotene alone or 1,25-dihydroxyvitamin $D_3$ alone caused a marked increase on the induction of apoptosis in EC9706 cells. The percentage of G0/G1-phase cells significantly increased on addition of 1,25-dihydroxyvitamin $D_3$ alone, but there were no significant changes with ${\beta}$-carotene alone. These two agents in combination synergistically inhibited cell growth and induced apoptosis. Therefore, our results indicate that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ in combination may provide a novel strategy for preventing and treating esophageal cancer.

Structure and Thermal-enhanced Magnetic Properties of Mn4C Melt-spun Ribbons with Varied Stoichiometry

Ting-Ting Qi,Ping-Zhan Si,Fang Cheng,Zhi-Rui Wang,Hong-Liang Ge,Qiong Wu,Jihoon Park,Chul-Jin Choi 한국자기학회 2023 Journal of Magnetics Vol.28 No.1

Cubic perovskite-type Mn4C is difficult to prepare for its metastable characteristics. In this work, we have obtained high-purity Mn4C successfully by using melt-spinning method. The effects of stoichiometry on the structure and magnetic properties of the samples were studied systematically. We found that x = -0.1 is the optimum composition for the formation of the cubic perovskite phase in Mn4+xC during rapid quenching. Most Mn4+xC melt-spun ribbons with x other than -0.1 are composed of Mn23C6, α-Mn, and Mn4C, while the fraction of different phase in Mn4+xC ribbons varies with x. The Curie temperature of Mn4+xC ribbons increases slightly with decreasing x, which may affect the lattice parameters of cubic Mn4C and thus the Mn-Mn exchange interactions. The magnetization of Mn4+xC (x = -0.1 and 0) increases with increasing temperature in high-temperature region while the onset temperature for such behavior is dependent on the fraction of Mn4C in the samples.

Two-way predation between larvae of Spodoptera frugiperda and Spodoptera litura

Wang Yuxue,Mbiza Natasha Isabel Tanatsiwa,Yu Yonghao,Zhang Haoran,Hu Zongwei,Liu Ting,Wang Yi,Zhang Yi,Luo Xincheng,Chu Longyan,Yang Yazhen,Wang Xiangping,Li Chuanren,He Hua,Zhang Jianmin 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.2

Spodoptera frugiperda has co-occurred with Spodoptera litura in corn fields since it invaded China on December 2018. In our process of rearing S. frugiperda and S. litura larvae in the lab, we discovered that when the larvae of S. litura and S. frugiperda were on the same host plant, two-way predation between them occurred. However, a detailed study on this phenomenon has not been previously reported. Therefore, the study on the two-way predation between larvae of S. frugiperda and S. litura was performed under mimicking natural conditions including the presence or absence of maize leaves. The results showed that 4th instar larvae of S. frugiperda could prey actively on the 3rd instar larvae of S. litura under natural conditions in the greenhouse, and there was twoway predation between S. frugiperda and S. litura larvae in either the presence or absence of maize leaves in the lab. In addition, the difference in the daily predation number of old S. frugiperda larvae (including 4th,5th and 6th instar) on S. litura 1st, 2nd and 3rd instar larvae respectively was significant between presence and absence of maize leaves. Similarly, the difference in the daily predation number of S. litura old larvae (including 4th, 5th and 6th instar) on S. frugiperda young 1st, 2nd and 3rd instar larvae respectively was very significant between presence and absence of maize leaves. These results confirm that two-way predation exists between larvae of S. frugiperda and S. litura, and S.frugiperda larvae are in a dominant position in this struggle, which provides an understanding of S. frugiperda feeding when coexisting with S. litura.

Photo-induced antifouling polyvinylidene fluoride ultrafiltration membrane driven by weak visible light

Ting Wang,Ling-li Jiang,Lu-lu Huang,Li-guang Wu,Chun-juan Li,Jing Cai 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.89 No.-

To enhance the performance of ultrafiltration membranes, a polyvinylidenefluoride (PVDF) blendingmembrane with photo-induced antifouling performance was fabricated. La3+- and Yb3+-doped titaniumdioxide-reduced graphene oxide (TiO2-RGO) composites were initially prepared by adsorption-phasesynthesis coupled with solvothermal reduction. The doped TiO2-RGO composites were then blendedwith the PVDF materials. The results showed that adding well-distributed, doped, and hydrophilic TiO2-RGO improved the pore structures and surface polarity of the blending membranes, thus improving theirpermeation and antifouling performance. After addition of doped TiO2-RGO with photocatalytic activity,the antifouling performance of the resulting blending membranes increased under weak visible-lightirradiation. The agglomerated doped TiO2-RGO in the polymeric matrix reduced the hydrophilicity andporosity of the resulting membranes, thereby decreasing their water permeability and antifoulingperformance. Solvothermal reduction through ethylene glycol also improved the photocatalytic activityof Yb-doped TiO2-RGO and enhanced the affinity between doped TiO2-RGO and PVDF. These two effectssimultaneously increased the water permeability and photo-induced antifouling performance of theblending membranes. Theflux recovery rate of the blending membranes containing Yb-doped TiO2-RGOtreated by ethylene glycol exceeded 69% after illumination. In particular, theflux recovery rate ofmembranes reached about 96% after illumination coupling with washing.