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- 저자 : Taye, Mengistie (검색결과 510 건)
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The potential applications of site‑directed mutagenesis for crop improvement: a review
Bezie Yilkal,Tilahun Tadesse,Atnaf Mulugeta,Mengistie Taye5 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.3
The search for technologies for crop improvement has been a continuous practice to address the food insecurity to the growing human population with an ever-decreasing arable land and dynamic climate change around the world. Considering the potential technologies for crop improvement could close the rooms of poverty in developing countries in particular and around the globe at large. This review aimed to assess the site-directed mutation creation methods and show the potential tools for future crop improvement programs. Site-directed mutagenesis was found to be an efcient process to create targeted mutation on cereal crops, horticultural crops, oilseed crops, and others. Agronomic traits such as yield, quality, and stress tolerance have been improved using site-directed mutagenesis. Besides, selectable marker elimination was also reported from transgenic crops by targeted mutation. Most of the reports on site-directed mutagenesis is focusing on cereal crops (58.339%) followed by horticultural crops (22.92%). Among the four mutagenic tools that have been reported, the CRISPR/ Ca9 technology was found to be frequently used (66.67%) followed by TALENs. This tool is potential, since it is efcient in creating targeted mutagenesis and less likely of-target efect, so it is repeatedly used in diferent research works. TALENs were used usually to knockout genes with bad traits. Moreover, the mutation created by mutagenic tools was found to be efcient, and the mutated traits proved to be heritable to generations. Hence, site-directed mutagenesis by the CRISPR/Cas9 system is advisable for agricultural development, thereby ensuring food sustainability around the world.
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Taye Saheed Kazeem,Mukarram Zubair,Muhammad Daud,Nuhu Dalhat Mu’azu,Mamdouh Ahmed Al-Harthi 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.7
Ternary layered double hydroxide, MgCoAl (MCA) and its graphene-based composite (G/MCA) were fabricated via a simple co-precipitation technique. The composites along with their calcined products (MCA-C) and (G/ MCA-C) were used as adsorbents for the removal of an anionic dye, methyl orange (MO), from aqueous phase. The characterization results (scanning electron microscopy and transmission electron microscopy) revealed homogeneous dispersion of graphene onto the MCA. Calcination of G/MCA resulted in a rough and heterogeneous surface with significant improvement in oxygen functionalities and surface area, which plays a crucial role in improved dye adsorption performance. Adsorptive equilibrium was established at 240 min for MCA and G/MCA and 180min for MCA-C and G/MCA-C respectively at pH 3 and optimum dosage of 10mg. The Redlich-Peterson and Langmuir isotherm models closely describe the adsorption process with maximum adsorption capacities of 357.14, 384.62, 400.12 and 434.78mg/g for MCA, G/MCA, MCA-C, and G/MCA-C respectively. Kinetics modeling indicates the adequacy and fitness of the pseudo-second-order model. A thermodynamics evaluation substantiates the exothermic nature of the adsorption processes. The MO-graphene ternary LDH composite adsorption process is controlled by several mechanisms including hydrogen bonding, surface adsorption, chemical and electrostatic interactions with surface reconstruction. The high removal efficiency of the MO coupled with high recovery and reusability of these nanomaterials showcases their potential for deployment in wastewater treatment.
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Taye, Adanech Melaku,Tilahun, Shimeles,Park, Do Su,Seo, Mu Hong,Jeong, Cheon Soon Korean Society of Horticultural Science 2017 원예과학기술지 Vol.35 No.3
'Unicon' cherry tomato (Solanum lycopersicum) is one of the most highly perishable horticultural crops due to its high water content and respiration rate. This study was carried out to assess the effect of continuous application of $CO_2$ (control [air], 3%, and 5%) on the quality and shelf life of cherry tomato fruits stored at $10^{\circ}C$ and $85{\pm}5%$ relative humidity (RH) at two maturity stages (pink and red). Continuous application of $CO_2$ did not affect the soluble solids content (SSC) or titratable acidity (TA) of the fruit at either maturity stage during storage. However, there was a significant difference among treatments in terms of flesh firmness, cell wall thickness, pectin content, vitamin C content, skin color, lycopene content, weight loss, ethylene production rate, respiration rate, and acetaldehyde and ethanol production. Fruits treated with 5% $CO_2$ maintained their high quality with regards to vitamin C, skin color ($a^*$), lycopene content, weight loss, physiological parameters (ethylene production rate, respiration rate, and volatile compounds), flesh firmness, cell wall thickness, and pectin content at both maturity stages compared with 3% $CO_2$ treatment and the control. Continuous application of $CO_2$ (5%) reduced the ethylene production rate and the production of volatile compounds during storage. Therefore, cherry tomato 'Unicon' fruit can be stored for two weeks without losing fruit quality at both maturity stages under continuous application of 5% $CO_2$ as a postharvest treatment.
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Kebede Taye Desta,윤혜명(Hyemyeong Yoon),신명재(Myoung-Jae Shin),이수경(Sukyeung Lee),Xiaohan Wang,최유미(Yu-Mi Choi),전영아(Young-ah Jeon),주영광(YoungKwang Ju),이정윤(JungYoon Yi) 한국작물학회 2023 한국작물학회지 Vol.68 No.3
Seed color is controlled by several genes and is a key trait in determining the metabolite content and biological activities of legume genotypes. In this study, 296 adzuki bean accessions, including 159 grey, 99 red, and 38 white adzuki beans, were grown in Korea. Variations in total phenolic content (TPC), total saponin content (TSC), DPPH• scavenging activity, ABTS•+ scavenging activity, and ferric reducing antioxidant power (FRAP) were assessed and were reported to be in the ranges of 1.52- 8.24 mg GAE/g, 14.36-114.22 mg DE/g, 0.23-12.84 mg AAE/g, 1.05-17.66 mg TE/g, and 0.59-13.14 mg AAE/g, respectively, with a wide variation across adzuki beans. Except for DPPH• scavenging activity, the average values declined in the order gray > red > white adzuki beans, each demonstrating a significant variation (p < 0.05). White adzuki beans, which showed low metabolite content and antioxidant activity, were clearly separated from the gray and red genotypes using principal component and hierarchical cluster analyses. Moreover, TPC, TSC, and antioxidant activities were strongly correlated, regardless of seed color. Overall, the diversity of the TPC, TSC, and antioxidant activity in a broad population of adzuki bean genotypes was determined. Furthermore, this study found that seed color variation in adzuki beans had a significant effect on the metabolite content and antioxidant activity. Superior accessions with high levels of TPC, TSC, and antioxidant activity were also discovered and could be used for functional plant breeding and human consumption. The findings of this study may be useful for understanding the relationship between seed coat color and metabolite concentration in adzuki beans, paving the way for molecular-level analyses.
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Kebede Taye Desta,최유미,이정윤,이석영,신명재,Xiaohan Wang,윤혜명 한국작물학회 2023 한국작물학회지 Vol.68 No.1
Adzuki beans have gained popularity in recent years due to their health benefits. Breeding of Adzuki beans is less favorable than with other legumes due to low genetic diversity. This study aimed to evaluate the genetic diversity of 252 adzuki bean germplasms from China, Japan, and Korea using 18 agro-morphological parameters and comparing their performance to three prominent Korean cultivars: Geomguseul, Arari, and Chungjupat. Leaf shape, pod color, and seed coat color were among the qualitative traits that showed wide variations. The quantitative variables also showed wide variations among adzuki bean germplasms. Although there was no significant difference (p < 0.05), the average rate of germination declined in the order of Korean (91.44%) > Chinese (91.31%) > Japanese (87.47%) adzuki beans. Chinese adzuki beans needed fewer days to flower (DF, 58.22 days) and days to mature (DM, 107.13 days), which varied significantly compared to the Korean and Japanese adzuki beans (p < 0.05). The average number of pods per plant (PPP) and one-hundred seeds weight (HSW) were higher in Japanese adzuki beans compared to the Korean and Chinese adzuki beans although the variation of each was not significant. Almost 29.76% of the accessions had early-blooming flowers, 3.97% were premature, 21.43% produced more PPP, and 3.97% yielded more SPP compared to control cultivars. Results of hierarchical cluster and principal component analyses revealed three clusters with significant variation in all quantitative variables except for RG (p < 0.05). The key factors in multivariate analyses were DF, DM, and HSW. Our study investigated the genetic diversity of adzuki bean accessions and identified ten early maturing and ten high PPP-yielding accessions. Our findings would help farmers and breeders to select the top-performing accessions that can provide them with various options.
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Kebede Taye Desta,전영아,신명재,최유미,이정윤,윤혜명 한국자원식물학회 2024 한국자원식물학회지 Vol.37 No.3
Abstract - This study investigated the diversity of 323 mung bean accessions from Korea, China, and India, along with six cultivars, using 22 agronomical traits. The standardized Shannon-Weaver index (H') for the qualitative traits ranged from 0.11 (terminal leaflet shape) to 0.98 (pubescence density of pod). Likewise, the coefficient of variation for the quantitative traits ranged from 8.76% (days to maturity (DM)) to 79.91% (lodging rate (LR)), indicating a wide genetic variance. Hypocotyl color, pod color, seed shape, and seed coat surface lust showed different distributions among Korean, Indian, and Chinese accessions. Chinese accessions had the highest average germination rate, DM, days from flowering to maturity, and one-hundred seeds weight, followed by Korean and Indian accessions, while the number of seeds per pod (SPP) displayed the opposite trend, with all except SPP showing significant variation (p < 0.05). Similarly, plant height, days to flowering, and number of pods per plant increased in the order of India > Korea > China, with LR showing the opposite trend (p < 0.05). The mung bean accessions were grouped into four major clusters using hierarchical cluster analysis supported by principal component analyses, and all of the quantitative traits showed significant variations between the clusters (p < 0.05). Generally, the mung bean accessions investigated in this study exhibited wide phenotypic trait variations, which could be beneficial for future genomics studies. Moreover, this study identified 77 accessions that outperformed the controls. Consequently, these superior accessions could provide a wide spectrum of options during the development of improved mung bean varieties.
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Kebede Taye Desta,최유미,전영아,신명재,윤혜명,Wang XiaoHan,오현석,나영왕,고호철,노나영,이정윤 한국작물학회 2024 한국작물학회지 Vol.69 No.2
ABSTRACT This study investigated 575 cultivated soybean landraces from different provinces in Korea, using 17 key agromorphological traits. The studied soybeans showed wide variations in both qualitative and quantitative traits, signifying the existence of genetic diversity. The standardized Shannon-Weaver index (H') ranged from 0.3 to 1.0, with seed-related traits having an H' value higher than 0.7. Similarly, quantitative traits showed significant variation, with the coefficient of variation ranging from 7.2% for days to maturity (DM) to 62.3% for the number of pods per plant (PPP). In terms of province, the Gyeongsangbuk-do and Gyeongsangnam-do accessions differed from the other accessions, with higher proportions of green and yellow seed coats and lower proportion of black hilums. Gyeongsangnam-do accessions also showed early maturation and flowering but had the lowest average one-hundred seeds weight (HSW). In contrast, Jeollanam-do accessions flowered and matured late but had the highest average seed weight per plant (SWPP). Hierarchical cluster analysis grouped the soybeans into 12 clusters, and further statistical analysis showed significant variations in all quantitative traits (p < 0.05). Principal component analysis grouped the accessions based on the clusters. DM, PPP, HSW, and SWPP were identified as major contributors to the observed variance along the axes of the first two principal components. Correlation analysis revealed significant associations between maturity and yield-related traits. Based on their relative performance, 37 promising accessions were identified. Overall, this study highlights the diversity of recently cultivated Korean soybean landraces and provides opportunities for future metabolomic and genomic studies.
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