단순 외상 환자에서 퇴원시 설명문이 환자의 이해도에 미치는 영향

장석준,정상원,최성욱,황태식,이한식 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

The many previous publications have shown the advantages of written discharge instruction over verbal discharge instruction on the basis of comparing the functional illiteracy among emergency department patients. Therefore, authors have conducted a study on the efficacy of written discharge instruction over the verbal discharge instruction of two groups: minor laceration and sprain patients. For the purpose of this study, minor laceration is defined as less than 3 ㎝ laceration on the face or head, or less than 5 ㎝ laceration on extremities. And simple sprains were limited to the extremities. A total of 416 patients who visited Yong Dong Severance Hospital Emergency Care Center were the subject of this study during the period of 2 months from Feb. 1. to Mar. 31. 1996. The study was done prospectively, and patients who entered this study were randomly selected. Simple discharge instruction were made prior to the study and patients were given or verbally told the one of two instruction sets by the emergency medicine resident. After reading or hearing the instructions, each patient was asked to answer four specific questions about them. Statistical significance was examined by t-test and p value of 0.05 or less was considered statistically significant. Mean age for verbal and written instructed group were 26.2 and 25.6 years old. There were 262 and 154 patients in verbal and written instructed group, and the mean score was 2.24±1.20 and 3.42±0.52, respectively. The type of injury was divided into laceration and sprain. Laceration group showed mean score of 2.15±1.23, 3.32±0.51, and sprain group of 2.34±1.16, 3.59±0.50 in verbal and written instruction group, respectively. For the patient younger than 13 years old, discharge instruction was given or described to a guardian. Age less than 13 years old group showed mean score of 2.75±1.11, 3.31±0.48 and more than 13 years old group of 2.10±0.19, 3.34±0.53 in verbal and written instruction group, respectively. The result showed statistical significance between verbal and written instruction group of type of injury and age difference. For the future, simplified written discharge instruction may help and improve the patient`s understanding of the proper management of injury.

Diversity of swine Bordetella bronchiseptica isolatesevaluated by RAPD analysis and PFGE

Tae-Wook Hahn,Eun-Kyung Shin,Yeon-Soo Seo,Jeong Hee Han 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.1

The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA- 07 which was shared by 32 isolates (71.1%), the pattern produced by OPA-08 was shared by 26 isolates (57.8%), and the pattern produced by OPA-18 was shared by 40 isolates (88.9%). The RAPD patterns of the vaccine strain and the 3 reference strains did not match any of the patterns produced by the field isolates when primers OPA-07 and OPA-08 were used. PFGE using the restriction endonuclease XbaI produced a total of 15 patterns consisting of 4 PFGE types (A, B, B1 and C, differing by ≥ 4 bands) and 11 A subtypes (differing by ≤ 3 bands). Most of the field isolates exhibited identical type A and B patterns, suggesting that they were related. The vaccine strain and the three reference strains showed different PFGE patterns as compared to the identical type A strains.

Booster vaccination with safe, modified, live-attenuated mutants of Brucella abortus strain RB51 vaccine confers protective immunity against virulent strains of B. abortus and Brucella canis in BALB/c mice

Hahn, Tae-Wook,Cho, Youngjae,Kim, Kiju,Truong, Quang Lam,Park, Bo-Kyoung Microbiology Society 2015 Microbiology Vol.161 No.11

GENOMIC DNA STRUCTURE AND NUCLEOTIDE SEQUENCES OF SOYBEAN PHY B GENE

Tae Wook Woo,Hak Soo Seo,Tae Ryong Hahn 생화학분자생물학회 1993 생화학분자생물학회 추계학술발표논문집 Vol.- No.-

MOLECULAR CLONING AND SEQUENCE ANALYSIS OF SOYBEAN PHYTOCHROME B GENE

Woo, Tae-Wook,Hahn, Tae-Ryong Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

A phytochrome gene clone (pB310) was isolated from a soybean genomic library by plaque hybridization with a cDNA clone (pFMD1) of Cucurbita phytochrome. The isolated clone (11kb) contains entire amino acid coding sequence including 5' and 3' noncoding regions. The clone was subcloned as 40 subclones and sequenced. Nucleotide and deduced amino acid sequence analysis suggest that the genomic structure of clone pB310 composed of five coding exons and four introns which is similar but a little distinct with other phy genes. Homology search analysis of the deduced amino acid sequence of the clone with those of other phy genes indicated that the pB310 was 69-74% homologous with phyB genes but 50-55% homologous with phyA and phyC genes, suggesting that soybean phy clone pB310 is categorized as B type phytochrome gene. The soybean phyB encodes total 1156 amino acids which is highly homologous with other phyB sequences except N-terminal region.

Evaluation of Safety and Efficacy of Bovine Mastitis Vaccine(StartvacⓇ) in Domestic Farm

Jae wook Joo,Yong Jae Kim,Jin hee Ohn,Myung Hyee Kim,Tae Wook Hahn 대한수의학회 2014 대한수의학회 학술대회발표집 Vol.2014 No.-

Ro 90-7501 inhibits PP5 through a novel, TPR-dependent mechanism

Hong, Tae-Joon,Park, Kwanghyun,Choi, Eun-Wook,Hahn, Ji-Sook Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Protein phosphatase 5 (PP5) is a serine/threonine phosphatase that belongs to the PPP family phosphatases. PP5 and the other phosphatases of the PPP family share significantly similar catalytic domain structure. Due to this structural similarity, natural competitive inhibitors such as okadaic acid and cantharidin exhibit broad specificity over the PPP family phosphatases. In this study, we report the identification of three PP5 inhibitors, Ro 90-7501, aurothioglucose, and N-oleoyldopamine, along with a novel inhibitory mechanism of Ro 90-7501. Unlike other inhibitors binding to the phosphatase domain, Ro 90-7501 inhibited PP5 in a TPR-dependent manner. This TPR-dependent PP5 inhibition shown by Ro 90-7501 is a unique and novel inhibitory mechanism, which might be a useful tool for studies of PP5 on both regulatory mechanism and drug discovery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ro 90-7501, aurothioglucose, N-oleoyldopamine were identified as PP5 inhibitors. </LI> <LI> Ro 90-7501 required the TPR domain of PP5 to inhibit PP5 efficiently. </LI> <LI> The TPR-dependent PP5 inhibition of Ro 90-7501 demonstrates a novel mechanism. </LI> </UL> </P>

Lymphovascular space invasion is highly associated with lymph node metastasis and recurrence in endometrial cancer

( Ho Suap Hahn ),( In Ho Lee ),( Tae Jin Kim ),( Ki Heon Lee ),( Jae Uk Shim ),( Jae Wook Kim ),( Kyung Taek Lim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

The purpose of this study was to evaluate whether lymphovascular space invasion (LVSI) is a prognostic factor for lymph node metastasis and relapse of disease in endometrial cancer. We retrospectively analyzed the medical records of 438 patients with endometrial adenocarcinoma treated by surgical staging, including pelvic and paraaortic lymph node dissection, between January 1996 and July 2011. One hundred sixty three patients (37.2%) were LVSI-positive and 275 (62.8%) were negative. LVSI-positive patients were statistically significantly older and showed more advanced stage, poorer differentiation, and a higher frequency of non-endometrioid histology type, myometrial invasion, and positive peritoneal cytology than LVSI-negative patients. Surgeries by laparotomy rather than laparoscopy and more adjuvant therapies were conducted in LVSI-positive patients. The median numbers of pelvic and paraaortic lymph nodes removed were not different, but LVSI-positive patients showed more lymph node metastases. The LVSI-positive group also showed a higher recurrence of disease and lower survival rates than the LVSI-negative group. Negative predictive values of LVSI for lymph node metastasis and recurrence of disease were 96.4 and 97.1%, respectively. In multivariate analysis, LVSI did not influence overall or disease-free survival after adjusting for several confounding factors. The presence of LVSI is highly associated with lymph node metastasis and relapse of disease in endometrial cancer, but dose not influence survival as an independent factor. Therefore, LVSI may be a valuable prognostic factor when used in conjunction with other risk factors.

Safety and efficacy of a inactivated multi-valent vaccine aganist the chicken Salmonellosis with Salmonella Enteritidis, Typhimurium, and Gallinarum.

( Soyeon Park ),( Tae-wook Hahn ) 대한인수공통전염병학회 2017 창립총회 및 학술대회 초록집 Vol.2017 No.1

Introduction: Salmonella Gallinarum(SG) is the causative agent of fowl typhoid in chickens while S. Enteritidis (SE) and S. Typhimurium (ST) are the causative agent of nontyphoidal foodborne disease in humans as well as chickens. However, Vaccine for protection of Salmonella three strains has not been used in poultry farms in worldwide. Therefore, our aim was to develop Salmonella inactivated vaccine which had higher safety and protection efficiency than commercial vaccine. Materials and Methods: Salmonella lipid A mutants were constructed using Red recombination system. The experimental groups consisted of 3 different vaccines and 4 control: multi-valent vaccine (G1-3), Salenvac-T (G4-5), and SG9R (G6) and challenge control (G7-9) groups. To estimate the protective efficiency of each type of vaccine, 3w old Lohmann brown chickens were intramuscularly immunized twice with each vaccine for 14 days interval. At 14 days after last vaccination, immunized chickens were orally challenged with virulent SE, ST and SG strains, and the efficacy of each vaccine was determined on 14-days post-challenge. The immune response was assessed using ELISA. Results: Protection study demonstrates that Salmonella multi-valent inactivated vaccine was safer and showed similar to that in commercial vaccine groups. Against salmonellosis in chickens. Moreover, IgG and IgM levels in multi-valent vaccine group showed similar to that in commercial vaccine group. Conclusion: The Salmonella multi-valent inactivated vaccine offer best protection after vaccination via the intramuscularly routes, and that showed similar immunogenicity and protection compared to the know product commercial vaccine. We suggest that Salmonella multi-valent inactivated vaccine is safe and can be used for better prevention of Salmonellosis outbreak in chicken farms. As, one dose of our vaccine has good effeicacy protection against SE, ST and SG infection. References 1. Meeusen ENT, Walker J, Peters A, Pastoret P & Jungersen G (2007) Current status of veterinary vaccines. Clin.Microbiol.Rev. 20:489-510.

응급 괴사조직 제거술을 병행하여 치료한 원발성 비브리오 패혈증 15예

신규태,이이형,황성철,김명욱,김명성,김관식,이선민,마경애,한명호 대한감염학회 1997 감염 Vol.29 No.4

목적: 비브리오 패혈증은 병력, 해산물의 생식력, 피부 병변의 분포 및 특징을 고려하면 임상적으로 진단이 가능하며, 종래의 항생제 치료만으로는 48시간 이내의 사망률이 60%정도되는 전격적인 경과를 취한다. 이에 연구자들은 항생제의 투여와 함께 광범위한 응급 괴사조직 제거술을 병행하는 치료가 질병의 경과에 영향을 주는지 알아보고자 하였다. 방법: 1994년 5월부터 1997년 4월까지 아주대학교 병원에 내원하여 임상적으로 비브리오 패혈증을 진단받은 15예(세균학적으로 증명된 8예를 포함)를 대상으로 하여 병합 항생제의 투여와 응급 괴사조직 제거술을 병행하여 치료하였다. 결과: 1) 15예중 8예에서 나중에 세균학적을 증명되었고 소인으로는 간경변 2예, 만성 간 질환 또는 음주벽 10예, 당뇨병 3예, 위절제술 1예가 있었고 4예에서는 기저 질환을 찾을 수 없었다. 2) 모든 환자는 피부 병변을 가지고 있었고, 1예를 제외한 모든 환자에서 저혈압, 혈소판 감소증, 간 부전, 신부전 및 의식혼돈을 보이고 있었고 평균 APACHE Ⅲ score(Acute Physiology, Age, Chronic Health Evaluation score Ⅲ)는 84점이었다. 3) 15예중 2예에서는 응급 수술을 시행하지 못했다. 1예에서는 전신에 괴저(gangrene)가 있었으며 1예에서는 전신에 산발적인 수포가 있어 절제범위가 너무 넓어져서 시행하지 못했다. 4) 전체 환자의 48시간 이내의 생존율은 80%(12/15명)였고, 장기 생존율은 66.6%(10/15명)였으며 세균학적으로 확진된 경우도 48시간 이내 생존율은 75%(6/8명)였고, 장기 생존율은 62.5%(5/8명)였다. 5) 사망한 군에서 생존한 군보다 내원 24시간 이내의 APACHE Ⅲ score가 높았고 혈청 알부민이 낮았다. 결론: 대조군이 없어 응급 괴사조직 제거술의 예후에 대한 영향을 증명할 수는 없었지만, 본 연구는 문헌상의 대조군을 고려해본다면 병합 항생제 투여와 함께 응급 괴사조직 제거술을 병행하는 것이 비브리오 패혈증으로 인한 단기, 장기 생존율을 향상시킬 가능성을 보여주었고 또한 혈청 알부민이나 APACHE Ⅲ score가 예후를 평가하는데 도움이 됨을 보였으나 이러한 가능성은 대조군이나 더 많은 증례에 의한 검증이 필요할 것으로 사료된다. Background:To evaluate the role of emergency debridement performed in conjunction with an empirical antibiotic therapy in clinically diagnosed, full-blown Vibrio vulnificus sepsis. Methods:Immediate surgical debridement was performed on 13 out of 15 patients who were clinically diagnosed as having Vibrio vulnificus sepsis. Results:Among 15 patients clinically diagnosed Vibrio vulnificus sepsis, Vibrio vulnificus was isolated in 8 patients. Underlying diseases were liver cirrhosis(2), chronic alcohol ingestion or chronic liver disease(10), diabetes mellitus(3), gastrectomy(1) and in 4 cases no underlying condition was identified. All patients had skin lesions such as erythema, bulla, vesicle and gangrene. All but one initially showed variable degree of hypotension, thrombocytopenia, hepatic dysfunction, renal failure and mental confusion. Their mean APACHE Ⅲ score was 84. Immediate survival (within 48hrs) in clinically diagnosed Vibrio sepsis was 80(12/15)% and long term survival rate among them was 66.6%. Forry eight hour survival rate in bacteriologically confirmed cases of Vibrio vulnificus sepsis was 75%, where their long term survival was 62.5% Conclusion:In treating full-blown Vibrio vulnificus sepsis, immediate emergency surgical debridement performed in conjunction with the empirical antibiotics gives a possibility to improve both immediate and long term prognosis of the disease.