신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

Single cellular cotransfection imaging cytometry for accurate multi-target-multi-drug analysis

Yu Kyung TAK,Pravin K. NAOGHARE,Joon Myong SONG 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.4

Early Stage High-Content HIV Diagnosis Based on Concurrent Monitoring of Actin Cytoskeleton, CD3, CD4, and CD8

Tak, Yu Kyung,Song, Joon Myong American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.9

<P>Antiretroviral treatment can reduce the death rate of human immunodeficiency virus (HIV) infection, and its effectiveness is maximized at the early stage of HIV infection. The present study demonstrates an early stage high-content HIV diagnosis based on multicolor concurrent monitoring of CD4, CD8, and CD3 coreceptors and F-actin cytoskeleton using quantum dot (Qdot)–antibody conjugates at the single cell level. Artificial HIV infection of peripheral blood mononuclear cells (PBMCs) was achieved by the treatment of PBMCs with gp120 glycoproteins. Using the present system, it was determined that the CD4/CD8 ratios of normal PBMCs obtained from the blood samples of 11 adults were in the range of 1.04 to 1.52 as a result of the quantitative counting of single PBMCs while the CD4/CD8 ratios of artificial HIV-infected PBMCs were from 0.045 to 0.63. In addition, the structural changes of actin filament alignments in PBMCs bound to gp120 proteins were clearly observed by the multicolor single cell imaging system. This approach suggests a new model of accurate early stage HIV diagnosis simultaneously providing information on actin cytoskeleton and subtypes of PBMCs as well as their CD4/CD8 ratios.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-9/ac303727e/production/images/medium/ac-2012-03727e_0004.gif'></P>

Maximum a posteriori CFAR Detector for wei bull clutter

Kyung-Tak Yu,Jin-Heon Seo 대한전기학회 1996 Journal of KIEE Vol.9 No.4

Single Cell Imaging Cytometry for Assessment of Drug-Induced Off Target Effect Based on Reporter Gene Assay

TAK Yu Kyung,NAOGHARE Pravin K.,SONG Joon Myong 大韓藥學會 2010 大韓藥學會 總會 및 學術大會 Vol.2010 No.2

Hypomethylation of Long Interspersed Nuclear Element-1 (LINE-1) Leads to Activation of CD133 and ST18 in Hepatocellular Carcinoma

( Yu Rim Lee ),( Kyunghwa Kim ),( Se Young Jang ),( Won Young Tak ),( Young Oh Kweon ),( Bina Jung ),( Gyoun Eun Kang ),( Sang Kyung Seo ),( Jung Gil Park ),( Hye Won Lee ),( Young Seok Han ),( Jae Mi 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Long interspersed nuclear element-1 (LINE-1) hypomethylation, representing global DNA methylation level, is associated with prognosis via activation of oncogenic functions of genes. This experiment was performed to evaluate prognostic implication of LINE-1 methylation in patients with hepatocellular carcinoma (HCC) and the possible mechanisms related to oncogene activation. Methods: Ninety-six HCC patients between October 2014 and September 2015 at Kyungpook National University Hospital, Daegu, South Korea were enrolled for this prospective study. Quantitative pyrosequencing was performed to quantify the methylation level of for CpG sites in the LINE-1 promotor. The expression of CD133 and ST18 were measured by immunohistochemistry and their correlation with LINE-1 methylation levels were analyzed. Results: LINE-1 was significantly hypomethylated in tumor tissues compared with nontumor tissues (64.0 ±11.6% vs. 75.6 ±4.0%, respectively, P<0.0001). In this study population, LINE-1 hypomethylation group had a large proportion of female gender, smaller tumor size, and nonexistence of ascites (P< 0.05). Contrary to previous reports, LINE-1 hypomethylation was not an independent risk factor for overall survival and disease progression (all P >0.05). A total of 81 (84.4%) patients had demethylation of LINE-1 (ΔMI<0), and 15 (15.6%) patients had hypermethylation of LINE-1 (ΔMI≥0). HCC with demethylation of LINE-1 (ΔMI<0) had higher CD 133 expression than HCC with hypermethylation of LINE-1 (ΔMI≥0) (P=0.011). Moreover, when patients divided into two groups based on the mean value of tumor line-1 methylation, ST18 showed borderline significance in distinguishing the LINE-1 hypomethylation group than the other (P=0.053). Conclusions: LINE-1 demethylation is associated with expression of CD133 and ST18 in Hepatocellular carcinoma. In this study, LINE-1 hypomethylation was not related to overall survival and disease progression, this is probably due to the enrollment of HCC patients with various tumor stages and liver function.

Association between Serum-Ferritin Levels and Sleep Duration, Stress, Depression, and Suicidal Ideation in Older Koreans: Fifth Korea National Health and Nutrition Examination Survey 2010–2012

Kyung Mi Kim,Hye Rim Hwang,Yun Jin Kim,Jeong Gyu Lee,Yu Hyeon Yi,Young Jin Tak,Seung Hun Lee,Sung In Chung 대한가정의학회 2019 Korean Journal of Family Medicine Vol.40 No.6

Background: Serum-ferritin levels may be associated with psychiatric symptoms among the elderly; however, this association has not been extensively studied. The aim of this study was to evaluate the association between serumferritin levels and mental health issues in older Koreans. Methods: This cross-sectional study included large-scale national data from the Korea National Health and Nutrition Examination Survey 2010–2012. In total, 1,802 older Koreans (≥65 years old) were included. The assessed psychiatric symptoms included short sleep duration, stress, depressed mood, and suicidal ideation. Any association between serum-ferritin levels and mental health issues according to sex was examined using logistic regression analysis. Results: The prevalence of short sleep duration, stress, depressed mood, and suicidal ideation was 50.9%, 21.7%, 18.2%, and 22.5%, respectively. In men, suicidal ideation decreased with increased serum-ferritin levels after multivariate adjustment for sociodemographic and lifestyle factors and comorbidities. The odds ratios (OR) and 95% confidence interval (CI) of suicidal ideation by quartiles of serum-ferritin level were 1 (reference), 1.132 (95% CI, 0.569–2.252), 0.453 (95% CI, 0.217–0.945), and 0.608 (95% CI, 0.295–1.253), respectively, (P for trend=0.039). In women, no trend was observed. However, compared to the lowest quartile, short sleep duration (<7 h) and stress perception were significantly decreased in the third quartile, with odds ratios of 0.601 (95% CI, 0.461–0.888), and 0.606 (95% CI, 0.386–0.952), respectively. Conclusion: Moderate serum-ferritin levels were associated with decreased risk for suicide ideation in men, and were associated with decreased short sleep duration and stress perception in women.

Differential DNA Methylation as a Potential Biomarker for Stratification of Liver Fibrosis in Patients with Non-Alcoholic Fatty Liver Disease

( Yu Rim Lee ),( Eunhye Lee ),( Se Young Jang ),( Won Young Tak ),( Young Oh Kweon ),( Bina Jung ),( Gyoun Eun Kang ),( Sang Kyung Seo ),( Jung Gil Park ),( Hye Won Lee ),( Young Seok Han ),( Jae Min 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Non-alcoholic fatty liver disease (NAFLD) can progress to advanced liver disease, but only in a minority of patients. Differential liver DNA methylation of peroxisome proliferator-activated receptor gamma (PPARγ) gene promoter has been shown to distinguish patients in terms of fibrosis severity in NAFLD. However, a study of methylation of PPARγ gene promoter in human with a sufficient number of patients is still scarce. We therefore determined the prognostic significance of DNA methylation of PPARγ gene promoter in patients with NAFLD. Methods: This study enrolled 54 biopsy proven NAFLD patients and 18 healthy controls who attended Kyungpook National University Hospital, Republic of Korea between March 2015 and October 2016. We extracted genomic DNA from liver tissue of enrolled patients. Bisulfite modification of genomic DNA was performed and PPARγ methylation level was confirmed with pyrosequencing. Results: The average of 4CpG methylation of PPARγ promoter had significantly lower in the NAFLD group (15.03% DNA methylation) when compared to the healthy controls (19.55%) (P=0.001). Quantitative DNA methylation of PPARγ stratified patients into mild (Kleiner 0-2) and severe (Kleiner 3-4) fibrosis (14.21% vs 17.87%, P=0.007). Moreover, hypermethylation at the PPARγ promoter of liver was also associated with higher age, the presence of DM, NAFLD fibrosis score, the degree of steatosis and fibrosis (P=0.015, 0.024, 0.012, 0.027, 0.009, respectively). However, they were not associated with presence and degree of inflammation, ballooning, NAFLD activity score, the presence of NASH, and other liver function test. Patients with advanced fibrosis exhibited significantly higher NAFLD fibrosis score, FIB-4, and PPARγ DNA methylation (all P<0.05) and showed borderline significance with transient elastography (P=0.084). These markers, including NAFLD fibrosis score, FIB- 4, and PPARγ DNA methylation, had an area under the receiver operating curve (AUROC) of 0.855, 0.778, 0.75, respectively for predicting advanced fibrosis. PPARγ mRNA was also related to the fibrosis and steatosis severity (P<0.05). This showed negative regulation with DNA methylation level (P=0.001). Conclusions: DNA methylation level of PPARγ may be useful in NAFLD patients for stratification and prediction of progressive liver fibrosis, which is a very important factor of NAFLD patients that is currently impossible to predict. It may be possible to generate an algorithm that can predict more precisely which patients are likely to progress on to a severe fibrosis in patients with NAFLD using DNA methylation and other markers related to liver fibrosis.

Prognostic Factors Affecting Treatment Outcomes of Acute Variceal Bleeding in Patients with Hepatocellular Carcinoma : A Single Center Prospective Study

( Yu Rim Lee ),( Soo Young Park ),( Won Young Tak ),( Young Oh Kweon ),( Se Young Jang ),( Jun Sik Yoon ),( Bina Jeong ),( Gyoun Eun Kang ),( Sang Kyung ),( Seo Keun Hur ),( Jung Gil Park ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Because acute variceal bleeding is associated with significant morbidity and mortality in cirrhotic patients, the prediction of prognosis is important. We therefore studied about prognostic factors affecting treatment outcomes of acute variceal bleeding. In addition, factors related acute variceal bleeding in patients with HCC were also determined with particular emphasis. Methods: Endoscopy-proven acute variceal bleeding patients admitted to the Kyungpook National University Hospital between 2007 and 2013 were enrolled in this prospective cohort study. Endoscopic procedures including either endoscopic variceal ligation or sclerotherapy with N-butyl-2-cyanoacrylate were performed for the patients within 24 hours after admission. The prognostic factors were identified by applying the multivariate Cox proportional-hazards regression test using significant variables in univariate analysis. Results: 329 patients were finally included in this study. During the study period (median=15.6 months), 186 patients at a median of 4.3 months were expired. Nineteen patients were expired in 5 days, which accounted for 38.8% of 6-week mortality (n=49). Six-week mortality was related to the MELD score and 5-day treatment failure in multivariate analysis in all patients and HCC subgroup (all P<0.05). When the patients were divided into two groups by MELD score, the risk of 6 week mortality was higher in patients with MELD ≥ 15.5 than in those with MELD < 15.5 (log rank test, P<0.001). We also found that 85% of patients with both MELD score ≥ 15.5 and terminal cancer stage including mUICC stage 4 or BCLC stage D expired within six weeks and 6-week mortality risk is about 10 times higher compared to patients with lower MELD score and earlier stage after adjusting other factors (P<0.05). Conclusions: Follow-up strategy and managements are required for the individual patients, depending on these risk factors. According to our study, overly enthusiastic endoscopic treatment in patients with poor liver function and end-stage HCC may be unnecessary.

A STUDY ON ANALYSIS AND USAGE OF WEB SITE FOR E-CARD

Tak Myung-Ja,Kim Yu-kyung,Jongchan Kim,Eungkon Kim,Kim Cheeyong 한국멀티미디어학회 2007 한국멀티미디어학회 국제학술대회 Vol.2007 No.-

Today's E-card can be compared to ready-made clothes. Many users are able to choose only a card among the ready-made flash E-cards. E-card suppliers have to make a lot of E-cards to fit with various users' needs and users also have to have a hard time searching for an appropriate E-card among them to send one card. Though an individualized multimedia service, which can be inserted user's picture and background music, is appearing now, the development speed and the service level are far from the users' demands. Not only can users send a modified E-card, but also receivers should be able to reuse the E-card after reconstructing it later. This is that E-card should be used applicably and continuously. E-card should be able to be printed and be appreciated with us for a long time. It is a compensation for the weakness of E-card which doesn't make us give possessiveness. This study offers users the usage to approach two-way communication of digital media. Users do not only select ready-made E-card passively, but also they can construct a web site interface to make an E-card directly.