Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

Song,Won-Seob,Chi,Hyung-Joon,Rim,Yo-Sup,Yoon,Jae-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.1

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis s rhizome and cultivated callus. In EtOAc, BuOH, H₂0separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC50, 35(g), BuOH(RC50, 43(g), H₂0(RC50, 50(g) fraction and MeOH extract(RC50, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC50, 14(g) and α-Tocopherol(RC50, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

人文·社會科學 系列의 大學 敎課科目에서 地域硏究로서의 「美國學」이 차지하는 그 內容과 範圍에 대한 하나의 分析的 評價硏究

柳盛根,梁琮懿,宋玄州,尹瑾燮,黃明澤 全北大學校 商科大學 附設 産業開發硏究所 1975 상과대학論文集 Vol.5 No.-

炭素纖維板으로 補强한 鐵筋콘크리트보의 構造的擧動에 관한 實驗的 硏究

김하진,고송균,박영배,조창호,김정섭 朝鮮大學校 建設技術硏究所 1998 建設技術硏究 Vol.18 No.1

This study is a part of research to improve the yield strength of concrete structure with a structural damage and conducts the experiment with the variables such as the period of restrengthening of specimen, restrengthening length (1.9m, 1.5m, 1.0m) and restrengthening effect according to restrengthening mehthod of restrengthening materials (nonrestrengthening, Anchor, Angle) to test bending behavior and yield strength performance of reinforced concrete beams restrengthened with carbon fiber laminate. As a result of experiment, in the restrengthening effects by the period of restrengthening, specimen restrengthened before loading, during reloading and after loading show 1.9m, 1.5m and 1.0m respectively, in the restrengthening effects according to restrengthening length, specimen restrengthened with 1.9m, 1.5m and 1.0m show 113.7%, 122.4% and 112.7% and in restrengthening effects according to restrengthening method of restrengthening materials, non-restrengthening and restrengthening in anchor and angle show 113.7%, 115.9% and 184.4% of increase in yield strength respectively. Accordingly, the specimen restrengthened during loading, the specimen with 1.5m of restrengthening and the specimen restrengthened with angle show high restrengthening effects respectively. With the above results, a crack is closed by elastic behavior in the case of test materials, but since in the actual concrete strucutre, a crack is not closed like the specimen during loading, the influence of it on restrengthening should be considered. It is found that although restrengthening effect by the restrengthening length secures a sufficient length, the increase of yield strength by restrengthening cannot be expected when bonded performance is not secured and in deciding restrengthening method of restrengthening materials, the method which have not damage to restrengthening materials and can be integrated with restrengthening materials and structure is effective.

鹽化物 含有梁에 따른 鐵分 腐蝕에 관한 實驗的 硏究

최진석,조철희,신용석,고송균,손순채,김정섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

As a result of separating river sand and sea sand into the corrosion increased according to the of thinnss or the coating and the Chloride Content and Corrosion of Steel Bar through the experiment, the following conclusions are obtained. 1. There was no corrosion in the case of treating the steel bar with anticorrosive among test specimens cured in the air for 60 days, corrosion was found in the test specimens not treated with anticorrosive and for 60 days of age. 2. The area of corrosion increased according to the thinnss of the coating and the chloride content wher exposed for are more than 60 days. Therefore, it was determined in general that the corrosion progressed more rapidly in water cured specimens than in air cured ones and this was due to the influence of C1_(￣) in the sea sand.

Development of a Theranostic Convergence Bioradiopharmaceutical for Immuno-PET Based Radioimmunotherapy of L1CAM in Cholangiocarcinoma Model

Song, In Ho,Jeong, Mun Sik,Hong, Hyo Jeong,Shin, Jong Il,Park, Yong Serk,Woo, Sang-Keun,Moon, Byung Seok,Kim, Kwang Il,Lee, Yong Jin,Kang, Joo Hyun,Lee, Tae Sup American Association for Cancer Research 2019 Clinical Cancer Research Vol.25 No.20

<P><B>Purpose:</B></P><P>Cholangiocarcinoma is a malignancy of bile duct with a poor prognosis. Conventional chemotherapy and radiotherapy are generally ineffective, and surgical resection is the only curative treatment for cholangiocarcinoma. L1-cell adhesion molecule (L1CAM) has been known as a novel prognostic marker and therapeutic target for cholangiocarcinoma. This study aimed to evaluate the feasibility of immuno-PET imaging–based radioimmunotherapy using radiolabeled anti-L1CAM antibody in cholangiocarcinoma xenograft model.</P><P><B>Experimental Design:</B></P><P>We prepared a theranostic convergence bioradiopharmaceutical using chimeric anti-L1CAM antibody (cA10-A3) conjugated with 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) chelator and labeled with <SUP>64</SUP>Cu or <SUP>177</SUP>Lu and evaluated the immuno-PET or SPECT/CT imaging and biodistribution with <SUP>64</SUP>Cu-/<SUP>177</SUP>Lu-cA10-A3 in various cholangiocarcinoma xenograft models. Therapeutic efficacy and response monitoring were performed by <SUP>177</SUP>Lu-cA10-A3 and <SUP>18</SUP>F-FDG-PET, respectively, and immunohistochemistry was done by TUNEL and Ki-67.</P><P><B>Results:</B></P><P>Radiolabeled cA10-A3 antibodies specifically recognized L1CAM <I>in vitro</I>, clearly visualized cholangiocarcinoma tumors in immuno-PET and SPECT/CT imaging, and differentiated the L1CAM expression level in cholangiocarcinoma xenograft models. <SUP>177</SUP>Lu-cA10-A3 (12.95 MBq/100 μg) showed statistically significant reduction in tumor volumes (<I>P</I> < 0.05) and decreased glucose metabolism (<I>P</I> < 0.01). IHC analysis revealed <SUP>177</SUP>Lu-cA10-A3 treatment increased TUNEL-positive and decreased Ki-67-positive cells, compared with saline, cA10-A3, or <SUP>177</SUP>Lu-isotype.</P><P><B>Conclusions:</B></P><P>Anti-L1CAM immuno-PET imaging using <SUP>64</SUP>Cu-cA10-A3 could be translated into the clinic for characterizing the pharmacokinetics and selecting appropriate patients for radioimmunotherapy. Radioimmunotherapy using <SUP>177</SUP>Lu-cA10-A3 may provide survival benefit in L1CAM-expressing cholangiocarcinoma tumor. Theranostic convergence bioradiopharmaceutical strategy would be applied as imaging biomarker-based personalized medicine in L1CAM-expressing patients with cholangiocarcinoma.</P>

항-Xg^a항체 1예 보고

허미나,이규만,김종현,박경운,송은영,한규섭 大韓輸血學會 2001 大韓輸血學會誌 Vol.12 No.2

Turn-on colorimetric sensing of fluoride ions by a cationic triarylborane bearing benzothiazolium

Song, Ki Cheol,Lee, Kang Mun,Kim, Hyungjun,Lee, Yoon Sup,Lee, Min Hyung,Do, Youngkyu Elsevier 2012 Journal of organometallic chemistry Vol.713 No.-

<P><B>Abstract</B></P><P>A cationic triarylborane Lewis acid bearing benzothiazolium moiety, 2-(4′-dimesitylborylphenyl)-3-methylbenzo[<I>d</I>]thiazol-3-ium ([<B>2</B>]<SUP>+</SUP>) was prepared from a neutral borane (<B>2a</B>) and their crystal structures were determined from X-ray diffraction studies. While <B>2a</B> shows a blue-shift with a small decrease in the UV–vis absorption band upon fluoride binding to the boron atom, [<B>2</B>]<SUP>+</SUP> undergoes a red-shift of the absorption band which tails over visible region, giving rise to the color change of the solution from colorless to yellow. The fluoride binding constant in THF/H<SUB>2</SUB>O (9/1, v/v) is calculated to be 1.3 × 10<SUP>4</SUP> M<SUP>−1</SUP> that is much greater than that of a neutral borane <B>2a</B> (<I>K</I> = 4.2 × 10<SUP>2</SUP> M<SUP>−1</SUP>). DFT calculation results suggest that the absorption process in the fluoroborate (<B>2</B>F) is involved with π(Mes)→π*(phenylbenzothiazolium) intramolecular charge transfer and the greater elevation of the π(Mes) donor level is responsible for the turn-on colorimetric response of [<B>2</B>]<SUP>+</SUP>.</P> <P><B>Graphical abstract</B></P><P>A cationic triarylborane Lewis acid bearing benzothiazolium moiety ([<B>2</B>]<SUP>+</SUP>) was prepared and characterized. [<B>2</B>]<SUP>+</SUP> displays enhanced fluoride ion affinity and turn-on colorimetric response toward fluoride.<ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► A novel cationic triarylborane Lewis acid was synthesized. ► Fluoride ion affinity was significantly enhanced. ► Fluoride binding led to turn-on color change from colorless to yellow.</P>

갑상선 미세침흡인세포검사 슬라이드에서 BRAF Mutation 검출을 통한 갑상선 유두암의 진단

박원서<SUP>1<,SUP>,이규언<SUP>2<,SUP>,송정윤<SUP>1<,SUP>,정유승<SUP>3<,SUP>,김훈엽<SUP>4<,SUP>,고석환<SUP>1<,SUP>,윤여규<SUP>2,5<,SUP>,Won Seo Park,<SUP>1<,SUP>,Kyu Eun Lee,<SUP>2<,SUP>,Jeong Yoon Song,Ph,D,<SUP>1<,SUP>,Y 대한갑상선-내분비외과학회 2010 The Koreran journal of Endocrine Surgery Vol.10 No.1

Purpose: The prevalence rate of the BRAF mutation in papillary thyroid cancer (PTC) is as high as about 52 to 83% in Korea. Preoperative detection of BRAF mutation on fine needle aspiration cytology (FNAC) slides may help the surgeon make better therapeutic decisions. The present study aims to assess the feasibility of the mutant allele specific amplification (MASA) and restriction fragment length polymorphism (RFLP) method with using conventional FNAC slides and we also wanted to evaluate the clinical role of preoperatively detecting BRAF mutation. Methods: We extracted the genomic DNA from 59 FNAC slides and performed direct sequencing (DS) for detecting BRAF mutation. We could use only 17 slides for the MASA method and 6 slides for the RFLP method due to the shortage of extracted DNA. Additionally, we retrospectively analyzed the cases for which a histological diagnosis could be made. Results: Genomic DNA was extracted from 23 out of the 59 FNAC slides. The BRAF mutation status could be assessed via DS in 33 out of the 59 FNAC slides. The concordance between the MASA method and DS and the RFLP method and DS was 36.3% and 66.7% respectively. The positive and negative predictive value of the 13 indeterminate nodules was 87.5% and 20%, respectively. We could not find any association between the BRAF mutations and the alleged risk factors of PTC. Conclusion: We believe that the purity and the amount of the DNA template must be increased to detect BRAF mutation with using a FNAC slide. Preoperative detection of the BRAF mutation on a FNAC slide may refine the cytological diagnosis, but the application of assessing BRAF mutation as a prognostic marker is debatable. (Korean J Endocrine Surg 2010;10:12-18)

Development of ⁶⁴Cu-NOTA-Trastuzumab for HER2 Targeting: A Radiopharmaceutical with Improved Pharmacokinetics for Human Studies

Woo, Sang-Keun,Jang, Su Jin,Seo, Min-Jung,Park, Ju Hui,Kim, Byoung Soo,Kim, Eun Jung,Lee, Yong Jin,Lee, Tae Sup,An, Gwang Il,Song, In Ho,Seo, Youngho,Kim, Kwang Il,Kang, Joo Hyun Society of Nuclear Medicine 2019 The Journal of nuclear medicine Vol.60 No.1

<P>The purpose of this study was to develop <SUP>64</SUP>Cu-labeled trastuzumab with improved pharmacokinetics for human epidermal growth factor receptor 2 (HER2). <B>Methods:</B> Trastuzumab was conjugated with SCN-Bn-NOTA and radiolabeled with <SUP>64</SUP>Cu. Serum stability and immunoreactivity of <SUP>64</SUP>Cu-NOTA-trastuzumab were tested. Small-animal PET imaging and biodistribution studies were performed in a HER2-positive breast cancer xenograft model (BT-474). The internal dosimetry for experimental animals was determined using the image-based approach with the Monte Carlo N-particle code. <B>Results:</B> <SUP>64</SUP>Cu-NOTA-trastuzumab was prepared with high radiolabeling yield and radiochemical purity (>98%) and showed high stability in serum and good immunoreactivity. Uptake of <SUP>64</SUP>Cu-NOTA-trastuzumab was highest at 48 h after injection as determined by PET imaging and biodistribution results in BT-474 tumors. The blood radioactivity concentrations of <SUP>64</SUP>Cu-NOTA-trastuzumab decreased biexponentially with time in both mice with and mice without BT-474 tumor xenografts. The calculated absorbed dose of <SUP>64</SUP>Cu-NOTA-trastuzumab was 0.048 mGy/MBq for the heart, 0.079 mGy/MBq for the liver, and 0.047 mGy/MBq for the spleen. <B>Conclusion:</B> <SUP>64</SUP>Cu-NOTA-trastuzumab was effectively targeted to the HER2-expressing tumor in vitro and in vivo, and it exhibited a relatively low absorbed dose due to a short residence time. Therefore, <SUP>64</SUP>Cu-NOTA-trastuzumab could be applied to select the right patients and right timing for HER2 therapy, to monitor the treatment response after HER2-targeted therapy, and to detect distal or metastatic spread.</P>

수술 전 혈청 갑상선 자극 호르몬(TSH)수치와 갑상선 유두암과의 상관관계

송금종,한선욱,이진형<SUP>1<,SUP>,우희두<SUP>2<,SUP>,김성용,김재우<SUP>3<,SUP>,박래경<SUP>1<,SUP>,백무준,김창호,Geum Jong Song,M,D,Sun Wook Han,M,D,Jin-Hyung Lee,M,D,<SUP>1<,SUP>,Hee-Doo Woo,M,D,<SUP>2<,SUP>,Sung Yong Kim,M,D,Ph 대한갑상선-내분비외과학회 2012 The Koreran journal of Endocrine Surgery Vol.12 No.3

Purpose: Recent research has shown that there is a relationship between the level of preoperative serum TSH and a papillary thyroid carcinoma. Therefore, this study examined the correlation between the serum TSH and papillary thyroid carcinoma. Methods: The preoperative serum TSH level of papillary thyroid carcinoma and nodular hyperplasia of 418 patients from 2009 Jan. to 2011 Dec. was examined. The patients were divided into 3 groups, nodular hyperplasia, less than 1 cm micropapillary carcinoma and more than 1 cm papillary carcinoma, and their TSH levels were compared. Results: Nodular hyperplasia and total papillary carcinoma was found in 98 (23.0%) and 322 (77.0%) patients, respectively. After dividing the patients according to the size of the mass, there were 224 (53.6%) patients with a mass less than 1 cm in size and 98 (23.4%) patients with a mass more than 1 cm in size. The preoperative serum TSH level of the 3 groups showed a significant difference, which was 1.180±1.168 ՌIU/ml in the nodular hyperplasia group, 1.670±1.224 ՌIU/ml in the micropapillary carcinoma group and 2.279±2.837 ՌIU/ml in the papillary carcinoma group (P<0.001). On the other hand, there were no significant correlations between the preoperative serum TSH level and gender, age, metastasis to lymph node, number of masses and extrathyroidal extensions. Conclusion: The larger size of the papillary thyroid carcinoma, the higher the preoperative high serum TSH level. Therefore, the stimulation of TSH can affect the progression of papillary thyroid carcinoma but more study will be needed.