Conservative approach to dens invaginatus

Sung-Yeop You,Won-Jun Shon 대한치과보존학회 2010 Restorative Dentistry & Endodontics Vol.35 No.S2

Lifespan and efficiency of ProTaper F2 with reciprocating motion in curved root canals

Sung-Yeop You,Won-Jun Shon,Woo-Cheol Lee 대한치과보존학회 2010 Restorative Dentistry & Endodontics Vol.35 No.S2

Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation

You, Seung Yeop,Park, Yong Seok,Jeon, Hyuk-Joon,Cho, Dong-Hyung,Jeon, Hong Bae,Kim, Sung Hyun,Chang, Jong Wook,Kim, Jae-Sung,Oh, Jeong Su Informa UK (TaylorFrancis) 2016 Cell Cycle Vol.15 No.12

<P>Cytokinesis is the final step in cell division that results in the separation of a parent cell into daughter cells. Unlike somatic cells that undergo symmetric division, meiotic division is highly asymmetric, allowing the preservation of maternal resources for embryo development. Beclin-1/BECN1, the mammalian homolog of yeast Atg6, is a key molecule of autophagy. As part of a class III phosphatidylinositol 3-kinase (PI3K-III) complex, BECN1 initiates autophagosome formation by coordinating membrane trafficking. However, emerging evidence suggests that BECN1 regulates chromosome segregation and cytokinesis during mitosis. Thus, we investigated the function of BECN1 during oocyte meiotic maturation. BECN1 was widely distributed during meiotic maturation forming small vesicles. Interestingly, BECN1 is also detected at the midbody ring during cytokinesis. Depletion of BECN1 impaired the cytokinetic abscission, perturbing the recruitment of ZFYVE26 at the midbody. Similar phenotypes were observed when PI3K-III activity was inhibited. However, inhibition of autophagy by depleting Atg14L did not disturb meiotic maturation. Therefore, our results not only demonstrate that BECN1 as a PI3K-III component is essential for cytokinesis, but also suggest that BECN1 is not associated with autophagy pathway in mouse oocytes.</P>

Acute Lymphoblastic Leukemia in Elderly Patients: A Single Institution`s Experience

( Dong Yeop Shin ),( In Ho Kim ),( Ki Hwan Kim ),( You Nak Choi ),( Seung Hoon Beom ),( Yae Won Yang ),( Yoo Joo Lim ),( Eun Young Lee ),( June Koo Lee ),( Ji Yeon Kim ),( Hyun Kyung Kim ),( Sung Soo 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.3

Background/Aims: We investigated the clinical characteristics and prognosis of elderly patients with acute lymphoblastic leukemia (ALL). Methods: We reviewed the clinical data, laboratory findings, bone marrow findings, and cytogenetic analysis of elderly patients (≥ 60 years) with ALL, and data of an additional 101 younger adult patients (< 60 years) with ALL were reviewed for comparison. Results: Twenty-six elderly patients (≥ 60 years) and 101 younger adult patients (< 60 years) with ALL were retrospectively enrolled. The median follow-up duration was 6.0 months (range, 0.4 to 113.2) in the elderly patients and 21.7 months (range, 1.0 to 122.7) in the adult patients. In total, 34.6% (9 patients) of the elderly patients and 24.8% (25 patients) of the adult patients had Philadelphia chromosome positive ALL. The overall complete remission (CR) rate was much higher in the younger than in the elderly patients (94.1% vs. 57.7%, p < 0.001). The median overall survival (OS) of the younger patients (< 60 years) was 26.3 months, whereas that of the elderly patients (≥ 60 years) was 10.3 months (p = 0.003). In the elderly patients with ALL, T cell lineage and the presence of lymphadenopathy were significant prognostic factors for OS in a univariate analysis (p = 0.033 and 0.041, respectively). Conclusions: The outcomes of Korean elderly patients with ALL were poor, and the shorter OS was mainly due to the low CR rate. T-cell lineage and the presence of lymphadenopathy were significant prognostic factors in Korean elderly patients with ALL.

주왕산국립공원의 탐방로 훼손현황과 환경피해도 평가

남엽 ( Yeop Nam ),유주한 ( Ju Han You ),허상현 ( Sang Hyun Heo ) 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.4

This study was carried out to maintain, manage and restore the trails by assessing the physical conditions, the patterns of deterioration and the impact rating class of the major trails of Juwangsan National Park. The major trails followed 4 routes including Jubong, Gamaebong, Janggunbong~Geumeungwangi and Woloe. The route length of Jubong was 4.6 ㎞; it was 3.6 ㎞for Gamaebong, 5.6 ㎞for Janggunbong~Geumeungwangi and 5.9 ㎞for Woloe. As for the physical conditions, Jubong was the widest and Janggunbong~Geumeungwangi was the narrowest in trail width. In terms of the bared trail width, Jubong was the widest and Janggunbong~Geumeungwangi was the narrowest. As for the depth of erosion, Gamaebong was the deepest and Woloe was the shallowest. Janggunbong~Geumeungwangi was the steepest and Gamaebong was the gentlest in the slope. Further, Gamaebong showed the highest soil hardness, and Woloe had the lowest hardness. In terms of the times of appearance of deterioration patterns, Jubong recorded 71 times, Gamaebong 62 times, Janggunbong~Geumeungwangi 63 times and Woloe 78 times. In case of the impact rating class, the trail erosion was similar to grades Ⅰand Ⅱgrade; the rest were mostlyⅠgrade and hence considered to be in a generally good condition. For the ongoing management of trails, a comprehensive study needs to be conducted in the light of various environmental factors. Also, a restoration plan for damaged trails should be established with consideration for the surrounding environment and ecosystem.

국내 각 지역의 시간율별 강우강도 분포

최동유(Dong-You Choi),성종엽(Jong-Yeop Sung),윤중현(Jung-Hyun Yun),노순국(Sun-Kuk Noh) 한국정보기술학회 2010 Proceedings of KIIT Conference Vol.2010 No.-

일반적으로 10㎓ 이상의 주파수 대역에서의 신호 전송 시 감쇠 요인은 대기중의 산소와 수증기에 의한 흡수와 강우, 눈, 우박, 안개, 구름 등의 자연적인 대기현상이 지배적이다. 이 중 가장 큰 영향은 강우로 인한 전파감쇠이다. 강우로 인한 전파감쇠는 강우강도에 따라서 감쇠정도가 다양하고, 강우강도는 공간적ㆍ시간적으로 그의 변동이 매우 다양하다. 따라서 강우강도에 의한 시간적ㆍ공간적 데이터의 확보와 정확한 강우강도의 추정은 정확하고 신뢰성 있는 링크설계를 위한 가장 중요한 요소이다. 본 연구에서는 서울을 포함한 9개 지역의 최근 5년간(2004~2008)의 디지털 강우강도 데이터를 확보하여 지역별 강우강도와 시간율의 관계를 경험적으로 분석하였다. In general, attenuation at frequencies over 10㎓ is mainly due to absorption by oxygen and water vapor, snow, and atmospheric gases. Among this factor rain become an important limiting factor. However, rain attenuation is highly influenced by rainfall intensity and it varies over time and space. In this paper, rainfall intensity eight measurement sites including seoul over recent 5 years is obtained and the regional between rainfall intensity and percent of time is analyzed.

화상 중환자실과 비화상 중환자실 폐 침윤 환자에서 기관지폐포 세척술 (BAL)을 통해 획득한 호흡기 검체에서 분리된 균주에 대한 항균제 내성 실태

김종엽 ( Jong Yeop Kim ),김철홍 ( Cheol Hong Kim ),박수희 ( Su Hee Park ),고유상 ( You Sang Ko ),김미정 ( Mi Jeong Kim ),강혜련 ( Hye Ryun Kang ),황용일 ( Yong Il Hwang ),박용범 ( Yong Bum Park ),장승훈 ( Seung Hun Jang ),우흥정 ( 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.62 No.6

연구배경: 원내 폐렴의 적절한 항균제 치료를 위해서는 원발 병소에서의 원인균 규명과 항균제에 대한 내성 유무 확인이 필수적이다. 본 연구는 원내 폐렴이 의심되는 경우 기관지내시경을 통한 정량적 기관지폐포 세척술(bronchoalveolar lavage, BAL) 배양검사를 시행하여 빈번하게 분리되는 균주와 이들의 항균제 내성 현황을 알아보고자 하였다. 방법: 2004년 1월부터 2006년 6월까지 한림의대 한강성심병원 화상 중환자실과 비화상 중환자실에서 폐 침윤의 원인규명 위해 BAL 검사를 시행하였다. 104 CFU/mL 이상의 균주가 분리되면 양성으로 판정하였다. 결과: BAL 검사 결과 화상중환자실 27예(77.1%), 비화상중환자실 22예(59.4%)에서 배양 양성이었다. 분리되는 주요 균주는 S. aureus, Acinetobacter species 및 P. aeruginosa였다. 화상중환자실에서 분리된 S. aureus의 경우 전부 MRSA(100%)였으며, 비화상중환자실에서는 71.4%가 MRSA로 보고되었다. P. aeruginosa의 경우 화상중환자실 및 비화상중환자실에서 amikacin 54.6% vs. 50.0%, ciprofloaxacin 100% vs. 50%, cefepime 90.9% vs. 50%, imipenem 72.7% vs. 50%, ceftazidime 45.5% vs. 25% 및 piperacillin/tazobactam 90.9% vs. 50%의 내성률을, Acinetobacter species의 경우는 amikacin 90.9% vs. 100%, ciprofloxacin 100% vs. 87.5%, cefepime 90.9% vs. 100%, imipenem 100% vs. 62.5%, ceftazidime 90.9% vs. 100% 및 piperacillin/tazobactam 81.8% vs. 62.5%의 내성률을 보여주었다. 결론: 화상중환자실과 비화상중환자실에서 원내 폐렴이 의심되는 환자의 원인균 분포와 약제 내성 현황을 알 수 있었으며 향후 항균제 치료 전략에 도움을 줄 것으로 사료된다. Background: Nosocomial pneumonia in an intensive care unit (ICU) is associated with a high mortality rate. Diagnosing a respiratory tract infection in critically ill patients is still difficult but detailed information for the pathogens is needed to establish an adequate antimicrobial treatment. This study examined the causative organisms and their antimicrobial resistance using bronchoalveolar lavage (BAL) from patients suspected of having pneumonia in the ICU. Methods: From January 2004 to June 2006, ICU patients with diffuse lung infiltration were prospectively enrolled. The BAL was used to diagnose the respiratory infection, with 104 ≥ organisms considered a positive result. The most common organisms and their antimicrobial resistances were analyzed from the quantitative BAL cultures in the burn ICU and non-burn ICU. Results: A total 72 patients were included, 35 (M 29, F 6) in the burn ICU and 37 (M 26, F 11) in the non-burn ICU. 27 patients (77.1%) in the burn ICU and 22 patients (59.5%) in the non-burn ICU met the criteria for a positive BAL culture. The major pathogens were Staphylococcus aureus, Acinetobacter species and Pseudomonas aeruginosa. All strains (100%) of Staphylococcus aureus isolated from BAL (9 cases) were methicillin-resistant (MRSA) in the burn ICU, but 5 strains (71.4%, 7 cases) were MRSA in the non-burn ICU. Regarding Pseudomonas aeruginosa, the rate of resistance to amikacin, ciprofloxacin, cefepime, imipenem, ceftazidime, piperacillin/tazobactam in the burn and non-burn ICU ranged from 45.5% to 90% and 25% to 50%, respectively. In addition, the rate of resistance of Acinetobacter species to the above drugs in the burn and non-burn ICU ranged from 81.8% to 100% and 62.5% to 100%, respectively. Conclusions: These results are expected to provide useful guidelines for choosing the effective empirical antimicrobial therapy in patients with lung infiltrations in the burn and non-burn ICU. (Tuberc Respir Dis 2007; 62: 506-515)

Programmed death-1 (PD-1)-dependent functional impairment of CD4(+) T cells in recurrent genital papilloma.

Chang, Dong-Yeop,Song, Sang Hoon,You, Sooseong,Lee, Jino,Kim, Jihye,Racanelli, Vito,Son, Hwancheol,Shin, Eui-Cheol Springer-Verlag Italia 2014 Clinical and experimental medicine Vol.14 No.3

<P>Genital papilloma is caused by human papilloma virus (HPV) infection and recurs frequently. Although T cells are known to play a critical role in the control of HPV infection and papilloma development, the function and phenotype of these cells in the lesion remain to be elucidated. In the present study, we examined the function and phenotype of CD4(+) T cells isolated from the lesions of primary (n = 9) and recurrent (n = 11) genital papillomas. In recurrent papillomas, the frequency of proliferating (Ki-67(+)) CD4(+) T cells was significantly reduced compared with primary papillomas. Cytokine production was evaluated by intracellular cytokine staining in anti-CD3/anti-CD28-stimulated CD4(+) T cells. CD4(+) T cells from recurrent lesions showed impaired production of IL-2, IFN-γ, and TNF-α. Of interest, the frequency of cytokine-producing CD4(+) T cells significantly correlated with the frequency of Ki-67(+)CD4(+) T cells. We also studied expression of programmed death-1 (PD-1), a T-cell exhaustion marker. The frequency of PD-1(+)CD4(+) T cells was significantly increased in recurrent lesions and inversely correlated with the frequency of cytokine-producing CD4(+) T cells. The functional significance of PD-1 expression was determined in blocking assays with anti-PD-L1, which restored cytokine production of CD4(+) T cells from recurrent lesions. Taken together, in recurrent genital papilloma lesions, proliferation, and cytokine production by CD4(+) T cells are impaired and the PD-1/PD-L1 interaction is responsible for the functional impairment of CD4(+) T cells.</P>

Design, construction and operation of lab scale cylindrical steam assisted gravity drainage model for heavy oil recovery

이철위,Nansuk You,Songhun Yoon,Wonkyu Lee,Heung Yeoun Lee,Sang-Yeop Park,Jae Heon Shim,Jong Soo Kim 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.6

Based on a theoretical background [1,2], a lab scale cylindrical SAGD (steam assisted gravity drainage)model was designed, constructed and operated. There are six different parts in the apparatus: (1) water supplier, (2)steam generator, (3) SAGD cylindrical model, (4) cooling system, (5) constant pressure maintaining system and (6)production system. Temperature, pressure and steam injection rate were controlled by computer, and product (mixture of oil and water) was collected/separated manually. Extra heavy oil (<10 cp at 200 oC) and glass bead (diameter 1.5mm) were mixed homogeneously for making porosity of 0.3 and applied for simulating oil sand. For obtaining optimum operation conditions of SAGD apparatus, several attempts were made. When the steam at high temperature (160-180 oC),high pressure (8-9 atm) was injected with 20-25 cc/min, cSOR (cumulative steam to oil ratio) of about 5 was obtained with oil recovery of 78.8%.

산업위생관리사 직무분석에 관한 연구

박종성 ( Jong Sung Park ),김현욱 ( Hyun Wook Kim ),김치년 ( Chi Nyon Kim ),심상효 ( Sang Hyo Sim ),임준택 ( Joon Taek Lim ),유성종 ( Sung Jong You ),유상엽 ( Sang Yeop You ),최충곤 ( Chung Gon Choi ),박정균 ( Jung Kyun Park ) 한국산업보건학회 2004 한국산업보건학회지 Vol.14 No.2