Effect of Cysteine on the Indirubin Production by Recombinant E. coli Harboring fmo Gene

Gui Hwan HAN,Seong Eun BANG,Sun Lee JU,Si Wouk KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.4

Vitamin D3 and Beta-carotene Deficiency is Associated with Risk of Esophageal Squamous Cell Carcinoma - Results of a Case-control Study in China

Huang, Gui-Ling,Yang, Lei,Su, Ming,Wang, Shao-Kang,Yin, Hong,Wang, Jia-Sheng,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: The aim was to evaluate roles of vitamin D3 (VD3) and beta-carotene (BC) in the development of esophageal squamous cell carcinoma (ESCC) in a high-risk area, Huai'an District, Huai'an City, China. Methods: 100 new ESCC diagnosed cases from 2007 to 2008 and 200 residency- age-, and sex-matched healthy controls were recruited. Data were collected from questionnaires, including a food frequency questionnaire (FFQ) to calculate the BC intake, and reversed phase high-performance liquid chromatography (RP-HPLC) was used to measure the serum concentrations of BC and VD3. Odds ratios (OR) and 95% confidence intervals (CI) were calculated in conditional logistic regression models. Results: The average dietary intake of BC was $3322.9{\mu}g$ (2032.4-5734.3) in the case group and $3626.8{\mu}g$ (1961.9-5827.9) in control group per capita per day with no significant difference by Wilcoxon test (p>0.05). However, the levels of VD3 and BC in the case group were significantly lower than in the control group (p<0.05). The OR values of the highest quartile and the lowest quartile of VD3 and BC in serum samples were both 0.13. Conclusion: Our results add to the evidence that high circulating levels of VD3 and BC are associated with a reduced risk of ESCC in this Chinese population.

김치의 발효과정 중 펙틴질과 조직감의 변화에 대한 젓갈과 Chitosan 첨가의 영향

이귀주,안선정 한국조리과학회 1995 한국식품조리과학회지 Vol.11 No.3

새우젓과 멸치젓 그리고 키토산을 첨가하여 김치를 제조하고 김치의 숙성과정 중 pH와 산도, 조직감의 변화 및 pectin 질의 변화를 알아보았으며 또한 젓갈, chitosan을 첨가한 김치에 대한 관능 평가를 실시한 결과는 다음과 같다. 1. 김치의 숙성과정 중 pH는 감소하였으며 멸치젓 첨가군 새우젓 첨가군 대조군 키토산 첨가군의 순서로 감소하였다. 산도는 대조군이 숙성초기 0.28%에서 숙성말기 0.97%로 증가하였으며 멸치젓 첨가군은 0.27%에서 1.06%로, 새우젓 첨가군은 0.27%에서 1.03%로, 키토산첨가군은 0.26%에서 0.79%로 증가하였으며 멸치젓 첨가군 새우젓 첨가군 대조군 키토산 첨가군의 순서로 증가하였다. 2. 압착 시험에 의한 김치의 조직감 변화에서 압착 변형력은 김치의 숙성과정 중 대조군은 5.0㎏∼1.6㎏로 멸치젓 첨가군은 6.0㎏∼1.4㎏로, 새우젓 첨가군은 6.5 ㎏∼1.5㎏로, 키토산 첨가군은 6.0 ㎏∼2.5㎏의 범위로 감소하였으며 멸치젓 첨가군 새우젓 첨가군 대조군 키토산 첨가군의 순서로 감소하였다. 3.배추의 AIS로 부터 펙틴을 추출하여 HWSP, HXSP, HCISP로 분획한 함량비는 HWSP는 대조군, 새우젓 첨가군, 멸치젓 첨가군 순으로 숙성과정 중 증가하는 경향을 보였으나 키토산 첨가군은 소량 감소하는 경향을 나타내었다. HXSP는 모든 첨가군에서 모두 증가하는 경향을 나타내었다. HCISP는 멸치젓 첨가군, 새우젓 첨가군, 대조군 순서로 감소하였다. 이와는 달리 키토산 첨가군은 증가하는 경향을 나타내었다. 4.김치의 조직감 함수에 관한 관능 검사를 실시한 결과 숙성 3일과 5 일에 hardness, crispness와 chewiness는 키토산 첨가군이 다른 첨가군들 보다 높은 점수를 얻었다. 또한 김치의 pH, 산도, 압착변형력과 김치의 조직감 함수에 대한 관능 검사 결과 가장 높은 상관관계를 나타낸 조직감 수는 crispness이었다. 로부터 pH와 압착변형력은 crisponess와 양의 상관 관계를 나타내었으며 산도와 crisponess와는 음의 상관관계를 나타내었다. 이상의 결과로부터 chitosan의 첨가는 김치의 숙성과정 중 압착변형력을 증가시킨 것으로 나타났다. 또한 펙틴질의 분포에 영향을 주어 HWSP는 감소하고 HXSP는 증가하였으며 HCISP는 감소시킨 것으로 나타났다. This study was conducted to examine the effects of fish sauces from shrimp and anchovy and chitosan on the changes in pH, acidity, texture, and pectin fraction during Kimchi fermentation. Also, we conducted sensory evaluation on the textural properties of various Kimchis. The results were as follows: During fermentation, pH was decreased in the order of fermented anchovy sauce, fermented shrimp and control. And acidity was increased in the same order. But the addition of chitosan retarded the decrease in pH and increase in acidity. The compression force of various Kimchis during fermentation was decreased in the rder of fermented anchovy sauce, fermented shrimp, control and the addition of chitosan. During fermentation, hot water soluble pectin (HWSP) of control, fermented shrimp and fermented anchovy sauce increased whereas HCI soluble pectin (HCISP) in there treatments decreasd. On the while, HWSP decreased and HCISP increased by addition of chitosan. Sensory scare for the texture parameters such as hardness, crispness and chewiness of various Kimchis after the 3rd and 5th days of fermentation showed that hardness, crispness and chewiness were higher in chitosan treatment than in other treatments. The pH and acidity, compression force of Kimchis were appeared to be most highly correlated with crispness, showing that pH and compression force gave positive correlation and acidity gave negative correlation with crispness respectively. From the above results, chitosan addition was observed to infuluence the textural properties of Kimchi and their pectic substance.

한국산 감자의 품종에 따른 갈색화반응에 관한 연구

김영선,이귀주 한국조리과학회 1992 한국식품조리과학회지 Vol.8 No.3

본 연구에서는 우리나라에서 수확한 감자의 갈변의 정도를 측정하고, 이들 갈변정도의 차이가 total phenol, tyrosine 함량 및 PPO 활성과 같은 감자의 성분조성 중 어느 요인과 관련이 있는지를 알아 보았다. 1. 품종별 감자의 갈변의 정도는 수미가 가장 높았으며 갈변이 시작되는 시기인 유도기간도 짧았다. 또한 남작과 수미가 대지마에 비해 갈변이 조금 더 빠르게 진행 되었다. 2. 품종별 감자의 total phenol함량은 수미가 755㎍/g으로서 가장 높았으며 남작이 430㎍/g으로서 가장 낮았다. 한편 아미노산 자동분석에 의한 tyrosine 함량은 남작과 수미가 각각 0.13㎎/100㎎으로서 같았으며 대지마는 0.09㎎/100㎎으로서 낮았다. 품종별 감자의 PPO활성은 catechol을 기질로 하였을때 높게 나타났으며 수미가 가장 높은 PPO 활성을 나타내었다. 3. 세가지 품종의 감자의 갈변의 정도와 성분조성 사이에는 각각 비교적 높은 상관 관계를 나타내었다. 한편 갈변의 정도가 가장 높은 수미는 PPO와 남작과 대지마는 tyrosine 함량 및 total phenol 함량과 각각 높은 상관 관계를 나타내었다. In this study, we measured the degree of browning of 3 potato culitivars harvested in Korea and compositions such as total phenols, tyrosine and polyphenol oxidase (PPO) activity. And we compared the extent of browning and composition factors to investigated which was related to the differences among 3 cultivars in browning. The results are as follows. 1. Among 3 culitivars, Sumi browned most after grinding and showed that browning in Sumi was preceded by significantly shorter lag time. Namjak and Sumi were rapid to brown compared to Daejima. 2. Among 3 culitivars, Sumi contained significantly more total phenols than did Namjak and Daejima. On the other hand, tyrosine contents by automatic analyzer of Sumi and Namjak were similar and that of Daejima was the lowest. PPO activities were higher when catechol was used as substrate and Sumi contained more PPO than did the other culitivars. 3. When we compared the browning behavior and composition of 3 culitivars, significant correlation was obtained between browning extent and total phenols, tyrosine and PPO. For the individual cultivar, Sumi which browned much extensively showed higher correlation between browning and PPO. On the other hand, the other cultivars showed higher correlation with total phenols and tyrosine, respectively.

일 대학병원 소아중환자실에서 Modified Glasgow Coma Scale 적용의 객관성 검증

송영주 ( Young Ju Song ),함귀선 ( Gui Sun Ham ),이선영 ( Sun Young Lee ),손인숙 ( Ihn Suk Son ),정유민 ( Yu Min Jung ),박보배 ( Bo Bae Park ),송민경 ( Min Kyung Song ) 서울대학교 간호과학연구소 2010 간호학의 지평 Vol.7 No.1

Purpose: Rapid and accurate assessment of impaired consciousness is very important, especially for critically ill patients. Therefore, the Glasgow coma scale (GCS) has been widely adopted in the assessment of adult and pediatric comas. But the GCS should not be used for small children who show various normal responses according to their developmental stage. Therefore, the objectivity of pediatric coma measurements was verified by applying the modified GCS to patients in a pediatric intensive care unit (PICU) at a university hospital in Korea. Methods: The level of consciousness was evaluated for 200 cases who were admitted to a PICU from July 4, 2009 to September 18, 2009 and could show a verbal response, with our modified GCS for children. In addition, and we confirmed the frequency analysis and Kappa statistics with SPSS/WIN 17.0. Results: Kappa statistics which show inter-observer reliability were very good for all components (eye opening, verbal, and motor score) and was good for total GCS scores. Conclusion: It is concluded that our modified GCS is reliable. Therefore, reliable assessment for the level of consciousness is clinically practicable if enough training is supported.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

Comparison of Major Monoterpene Concentrations in the Ambient Air of South Korea Forests

Oh, Gil-Young,Park, Gui-Hwan,Kim, Ik-San,Bae, Ju-Soon,Park, Hye-Young,Seo, Yun-Gyu,Yang, Soo-In,Lee, Joong-Ki,Jeong, Sun-Ho,Lee, Wan-Jin Korean Society of Forest Science 2010 한국산림과학회지 Vol.99 No.5

The concentration of monoterpenes (12 species) was measured from spring to fall in 2008 in ambient air at six different forests located in the southern parts of Korea. Sampling - using a Solid Adsorption Tube - was conducted at 2-hour-interval a day (24 hours) of each season. The highest annual average concentration of total monoterpene was 0.715 ppbv, which occurred at Mt. Baega. ${\alpha}$-Pinene, sabinene and ${\beta}$-pinene were the most abundant compounds throughout the sampling periods and areas. The concentrations of monoterpene were higher in spring than in any other seasons. The compositions and concentrations of monoterpene displayed a distinct pattern by area. Most of monoterpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. This may be due to the fact that the amount of monoterpenes emitted increases at elevated ambient temperatures and increased sunlight intensity. The results of this study showed that each area had distinct monoterpene compositions, which depended on the tree species, topography and local climate at each area.

Research Articles : Comparison of Major Monoterpene Concentrations in the Ambient Air of South Korea Forests

( Gil Young Oh ),( Gui Hwan Park ),( Ik San Kim ),( Ju Soon Bae ),( Hye Young Park ),( Yun Gyu Seo ),( Soo In Yang ),( Joong Ki Lee ),( Sun Ho Jeong ),( Wan Jin Lee ) 한국임학회 2010 한국산림과학회지 Vol.99 No.5

The concentration of monoterpenes (12 species) was measured from spring to fall in 2008 in ambient air at six different forests located in the southern parts of Korea. Sampling-using a Solid Adsorption Tube-was conducted at 2-hour-interval a day (24 hours) of each season. The highest annual average concentration of total monoterpene was 0.715 ppbv, which occurred at Mt. Baega. a-Pinene, sabinene and β-pinene were the most abundant compounds throughout the sampling periods and areas. The concentrations of monoterpene were higher in spring than in any other seasons. The compositions and concentrations of monoterpene displayed a distinct pattern by area. Most of monoterpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. This may be due to the fact that the amount of monoterpenes emitted increases at elevated ambient temperatures and increased sunlight intensity. The results of this study showed that each area had distinct monoterpene compositions, which depended on the tree species, topography and local climate at each area.

Thermal Properties of 0.9CaMgSi₂O₆-0.1MgSiO₃ Glass-Ceramics

Jeon, Chang-Jun,Sun, Gui-Nam,Lee, Jong-Kyu,Ju, Han-Sae,Kim, Eung-Soo The Korean Ceramic Society 2012 한국세라믹학회지 Vol.49 No.1

Dependencies of thermal properties on the crystallization behavior of $0.9CaMgSi_2O_6-0.1MgSiO_3$ glass-ceramics were investigated as a function of heat-treatment temperature from $750^{\circ}C$ to $950^{\circ}C$. The crystallization behavior of the specimens depended on the heat-treatment temperature, which could be evaluated by differential thermal analysis (DTA), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analysis by the Rietveld-reference intensity ratio (RIR) combined procedure. With an increase of the heat-treatment temperature, the thermal conductivity and thermal diffusivity of the heat-treated specimens increased. These results could be attributed to the increase of crystallization with heat-treatment temperature. However, the specific heat capacity of the heat-treated specimens was not affected by the heat-treatment temperature. The thermal conductivities measured from $25^{\circ}C$ to $100^{\circ}C$ were also discussed for application to lighting-emitting diode (LED) packages and substrate materials.

간호대학생의 임상실습교육 질적 개선을 위한 정책적 지원과 탄력적 운영 방안

양경희(Kyung Hee, Yang),최은주(Eun Ju, Choi),박순옥(Soon Ok, Park),고순희(Soon Hee, Ko),최귀윤(Gui Yun, Choi),박종덕(Jong Duk, Park),박선정(Sun Jung, Park) 한국간호연구학회 2020 한국간호연구학회지 Vol.4 No.2

Purpose : This study aims to develop practical standards and models and present operational plans so that clinical practice education for nursing students can be operated efficiently and quality improvement is achieved. Methods : The study was designed as a systematic review of citations or analysis of information published articles on the theme and related national administrations. Results : First, 1,000 hours of clinical practice consists of 4∼6 credits in school training and 20∼21 credits of clinical field training. Second, it is based on essential credits of each clinical subject and considers flexible operation. Third, it is necessary for the Ministry of Education to support job retraining every 2 years and to establish a system, and gives practical instructor of institution 2 positions by clinical/educational institutions. Fourth, the Ministry of Health and Welfare should state the duty of training for nursing students, reflect it in institutional evaluations, and apply a dedicated nurse education program for new nurse education to nursing student. Fifth, in hospitals with more than 200 beds, and in the case of obstetrics and pediatrics, if there are specialists and educational nurses, even a clinic should be recognized as an institution for clinical nursing practice. Conclusion : 1,000 hours of clinical practice, including in school practice, can be maintained, which requires reasonable government support and cooperation between relevant national administrations and nursing professionals.