2003년도 국내 도축장에서 분리한 세균의 항생제 감수성 조사 1. 도축장의 식육으로부터 분리한 E. coli의 항생제 감수성

송시욱 ( Si Wook Song ),정석찬 ( Suk Chan Jung ),김성일 ( Sung Il Kim ),정명은 ( Myeong Eun Jung ),김계희 ( Kye Hi Kim ),이지연 ( Ji Yeon Lee ),임숙경 ( Suk Kyung Lim ),이영주 ( Young Ju Lee ),조남인 ( Nam In Cho ),박종명 ( Jong M 한국수의공중보건학회 2004 예방수의학회지 Vol.28 No.4

Genetic Relationship of Hibiscus syriacus L. Clarified by AFLP and Morphological Evaluation

Si-Yong Kang,Sang Hoon Kim,Wook-Jin Kim,Geung-Joo Lee,Hi-Sup Song,Dong Sub Kim,Jin-Baek Kim 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.6

Genetic diversity and relationship of Hibiscus syriacus L. were analyzed using 127 cultivars and 7 landraces by the amplified fragment length polymorphism (AFLP) and the assessment of morphological characteristics. The AFLP analysis was carried out by using capillary electrophoresis with 28 fluorescent labeled primer combinations. Morphological evaluation was executed by the 11 parameters. A multivariate data analysis was performed using similarity coefficients, Euclidean distance, unweight pair group method with arithmetic averages (UPGMA) clustering method, and principal coordinate analysis (PCoA) of NTSYS-pc program. In the AFLP analysis, a total of 2, 842 bands were produced in the 28 primer sets, and 43.4% (1,236 bands) of them were identified to be polymorphic. Similarity coefficient values ranged between 0.49 and 0.98. Scattered distribution of the 127 cultivars and 7 landraces was identified, and they were divided into three major groups based on PCoA and UPGMA clustering analysis. In the morphological evaluation, Euclidean distance values ranged from 0.28 to 10.32. The distribution showed that there was a little difference compared with the result of AFLP. The pedigrees of several cultivars were cross-correlated using the results of AFLP and morphological analysis.

RPL36 as a prognostic marker in hepatocellular carcinoma

Song, Myeong Jun,Jung, Chan Kwon,Park, Chung‐,Hwa,Hur, Wonhee,Choi, Jung Eun,Bae, Si Hyun,Choi, Jong Young,Choi, Sang Wook,Han, Nam Ik,Yoon, Seung Kew Blackwell Publishing Asia 2011 Pathology international Vol.61 No.11

<P>Ribosomal proteins (RP) play key roles in the regulation of apoptosis, multidrug resistance and carcinogenesis. The aim of this study was to investigate the expression of ribosomal protein L36 (RPL36) in hepatocellular carcinoma (HCC) and to correlate it with clinicopathological parameters and clinical outcome. Liver specimens were obtained from 60 HCC patients who had undergone a partial hepatectomy. Expression of RPL36 in tumor tissue and surrounding non‐tumorous tissues was evaluated on a tissue microarray by immunohistochemistry. RPL36 was expressed in 45 of 60 (75%) HCC by immunohistochemistry, but was not detected in corresponding non‐tumors. RPL36 expression correlated significantly with serum levels of albumin (<I>P</I>= 0.044) and prothrombin time (<I>P</I>= 0.026), which reflect liver synthetic function. Moreover, expression of RPL36 was found to be higher in patients with early tumor stages (I/II) (<I>P</I>= 0.038) or without portal vein thrombosis (<I>P</I>= 0.005). In univariate analysis, patients with RPL36 expression revealed better overall survival (<I>P</I>= 0.037). By multivariate survival analysis, RPL36 expression was found to be an independent prognostic factor for overall survival (<I>P</I>= 0.026). Our data suggest that RPL36 may be involved in the early stage of hepatocarcinogenesis, and it can be used as an independent and potential prognostic marker for resected HCC.</P>

Purification and Some Properties of a Blue Copper Protein from Methylobacillus Sp. Strain SK1 DSM 8269

Si Wook Kim,Jeong Ae Kim,김응빈,Young Tae Ro,Taeksun Song,Young Min Kim 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.2

A blue protein was purified from the Methylobacillus sp. strain SK1 that is grown on methanol in the pres-ence of copper ion. This protein was found to be a monomer with a molecular weight of 13,500. The Isoelectric point of the protein was estimated to be 8.8. The spectrum of the protein that was treated with fer-ricyanide showed a broad peak around 620 nm, but that of the dithionite-treated protein revealed no peaks. It contained 0.83 mol of EDTA-stable copper per mol protein. Under air, the protein accelerated the inacti-vation of methanol dehydrogenase (MDH). The pro-tein was reducible by phenazine methosulfate or by active MDH that was prepared from cells that were grown in the absence of added copper, but not by methanol, dichlorophenol indophenol, or inactive MDH that was prepared from cells that were grown in the presence of added copper. It was also reducible by active MDH in the presence of methanol. The absorp-tion peak at 340 nm of the active MDH disappeared after the enzyme was treated with ferricyanide, hy-drogen peroxide, or the purified blue protein. The in-active MDH also showed no peak at 340 nm. The 340-nm peak was not recovered after incubation of the inactive MDH and blue protein-treated active MDH with dithionite or methanol. The inactive MDH and blue protein-treated active MDH co-migrated with the active MDH preparation on nondenaturing poly-acrylamide gel, and contained two non-identical sub-units with molecular weights that were identical to those of the active MDH. The N-terminal amino acid sequence of the protein was Ala-Gly-Cys-Ser-Val-Asp-Val-Glu-Ala-Asn-Asp-Ala-Met-Gln-Phe. An analysis of the amino acid composition revealed that the pro-tein contained no tryptophan. It contained three cys-teines per mol protein. The blue protein in Methyloba-cillus sp. strain SK1 was produced only in the cells that were grown in the copper-supplemented medium.

Assessment of Respiratory Tumor Movement using 4D Computed Tomography for Stereotactic Radiosurgery in Lung Tumor

Song, Si Yeol,Park, Sung Ho,Yoon, Sang Min,Kim, Young Seok,Kim, Jong Hoon,Ahn, Seung Do,Shin, Seong Soo,Lee, Sang-wook,Park, Charn Il,Choi, Eun Kyung Korean Association for the Study of Lung Cancer (K 2007 Journal of Lung Cancer Vol.6 No.1

Effects of Low-temperature tumbling on the Quality Characteristics of Restructured Chicken Breast Ham

Si Young Kim,Ji Hun Choi,Yun Sang Choi,Hack Youn Kim,Kwang Il Ahn,Hyun Wook Kim,Tae Hyun Kim,Dong Heon Song,Cheon Jei Kim 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.3

The aim of this study was to evaluate the effects of tumbling condition (time and temperature) on the quality characteristics of restructured chicken breast ham. Tumbling conditions were 10, 30, and 60 min at 3 or -3oC, respectively. After tumbling, quality characteristic regarding the treatments processed by each condition were measured. There were no significant differences in pH levels among tumbling time or temperature. Treatments involving 60 min of tumbling had significantly higher water holding capacity (WHC) than that tumbled for 10 min, but WHC was not affected by the tumbling temperature. The tumbling condition at -3oC for 60 min demonstrated the most effective cooking yields for restructured chicken breast ham. Myofibrillar protein solubility of treatments tumbled for 60 min had the highest value of all, regardless of tumbling temperature. The hardness of treatments tumbled at -3oC were lower than those at 3oC; however, the springiness of treatments tumbled for 60 min were significantly higher than those regarding the treatments tumbled for 10 and 30 min. Therefore, tumbling technology for 30 min or more at -3oC can produce restructured chicken breast ham of excellent quality.

외음부에 발생한 Bowen병 1예

송지연,명재욱,임진수,박철종,김시용 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.1

Bowen's disease was originally described by Bowen in 1912 and is believed to be a squamous cell carcinoma in situ. It develops on any part of the .3kin surfaces or mucous membranes. But the lesions on the vulvar region are relatively ran. The gross appearance of vulvar lesion, which shows erosion, ulceration or pigmentation, is different from that of typical Bowen's disease. We, herein, report a case of Bowen's disease arising on a vulvar region with a review of the literature.(Korean j Dermatol 2003:41(1) : 112∼115)

비인강암에서 방사선치료의 예측인자로써 Ku70 발현의 의의

송시열(Si Yeol Song),이상욱(Sang-wook Lee),유은실(Eunsil Yu),조경자(Kyung-Ja Cho),박진홍(Jin-hong Park),김상윤(Sang Yoon Kim),남순열(Soon Yuhl Nam),이봉재(Bong-Jae Lee),김성배(Sung Bae Kim),최승호(Seung-Ho Choi),안승도(Seung Do Ahn) 대한방사선종양학회 2005 Radiation Oncology Journal Vol.23 No.1

목 적: 방사선치료를 시행 받은 비인강암 환자에서 Ku70과 DNA-PKcs의 발현 정도가 국소제어율과 치료 실패 양 상에 미치는 영향을 알아보고자 하였다. 대상 및 방법: 1995년 6월부터 2001년 12월까지 서울아산병원에서 방사선치료 단독 또는 동시항암화학-방사선치료를 시행 받은 66명의 원격전이가 없는 비인강암 환자를 후향적으로 분석하였다. 진단 시 얻은 조직검사 표본에서 Ku70과 DNA-PKcs의 면역활성도를 분류하였는데, 그 기준은 면역활성도가 50% 이상 Ku (+)과, 50% 미만 Ku(-) 이었다. Ku70과 DNA-PKcs의 면역활성도와 방사선치료에 대한 종양의 반응 정도와 재발양상과의 상관관계를 알아보았으며, 비인강암의 국소제어율에 영향을 미치는 예후인자를 알아보기 위하여 단변량분석을 시행하였다. 결 과: Ku (-) 환자에서 Ku (+) 환자보다 높은 5년 국소제어율(85% vs. 42%, p=0.042)을 보였으나, 원격전이율은 두 집단간에 차이를 보이지 않았다(78% vs. 82%, p=0.8672). 단변량분석결과 Ku70의 과발현이 기존에 알려진 비인 강암의 예후인자보다 우위에 있음을 알 수 있었다. 국소재발을 보였던 22명의 환자 중 18명의 환자에서 Ku70 (+) 으로 관찰되었다. DNA-PKcs의 발현 정도는 방사선치료 결과에 영향을 미치지 않는 것으로 나타났다. 결 론: Ku70은 원격전이가 없는 비인강암 환자에서 방사선치료 단독 또는 동시항암화학-방사선치료 시행 이후 종양의 반응과 국소제어율을 예측할 수 있는 분자생물학적인 예측인자로 사용될 수 있을 것으로 생각한다. Purpose: The objective of this study was to determine whether the expressions of the two components of DNA-dependent protein kinase, Ku70 and DNA-PKcs, influence the response to radiotherapy (RT) and outcome of treatment of non-disseminated nasopharyngeal carcinoma (NPC) in patients who received definitive RT. Materials and Methods: Sixty-six patients with NPC who were treated with radiotherapy alone or with concurrent chemotherapy between June 1995 and December 2001 were divided into groups based on the levels of immunoreactivity for Ku70 and DNA-PKcs in pretreatment biopsy specimens. The over-expression of Ku70 or DNA-PKcs groups included patients whose biopsy specimens showed at least 50% immunopositive tumor cells; patients in which less than 50% of the tumor cells in the biopsy tissues were immunopositive were placed in the low Ku70 and DNA-PKcs groups. The immunoreactivities for Ku70 and DNA-PKcs were retrospectively compared with the sensitivity of the tumor to radiation and the patterns of therapy failure. Univariate analyses were performed to determine the prognostic factors that influenced locoregional control of NPC. Results: The five-year locoregional control rate was significantly higher in the low Ku70 group (Ku(-)) (85%) than in the high Ku70 group (Ku(+)) (42%) (p=0.0042). However, there were no differences in the metastases- free survival rates between the two groups (Ku70 (+), 82%; Ku70 (-), 78%; p=0.8672). Univariate analysis indicated that the over-expression of Ku70 surpassed other well-known predictive clinocopathologic parameters as an independent prognostic factor for locoregional control. Eighteen of 22 patients who had locoregional recurrences of the tumor displayed an over-expression of Ku70. No significant association was found between the level of DNA-PKcs expression and the clinical outcome. Conclusion: Our data suggest that the level of Ku70 expression can be used as a molecular marker to predict the response to RT and the locoregional control after RT and concurrent chemotherapy in patients with non-disseminated NPC.

진행된 하인두암에서 유도항암화학요법 후 방사선치료의 결과

송시열(Si Yeol Song),김성배(Sung Bae Kim),김상윤(Sang Yoon Kim),남순열(Soon Yuhl Nam),최은경(Eun Kyung Choi),김종훈(Jong Hoon Kim),안승도(Seung Do Ahn),제형욱(Hyoung Uk Je),이상욱(Sang-Wook Lee) 대한두경부종양학회 2008 대한두경부 종양학회지 Vol.24 No.2

Purpose :To know the results after induction chemotherapy followed by curative radiation therapy for locally advanced hypopharyngeal cancer Methods and Materials :From August 1990 to December 2003, forty patients who were treated with in-duction chemotherapy and curative radiation therapy were analyzed retrospectively. Median age of patients was 60 years(range:40-78 years) and clinical stage was wholly stage 3 or 4. Induction chemotherapy used cisplatin with 5-FU or docetaxel, and its interval was 3 weeks. Irradiated radiation dose was 70 to 78Gy (median:70.8Gy). Results :Median follow-up time was 39.4 months(range:8-115 months). Treatment failures were ob-served in 52.5% patients, and main failure pattern was local recurrence in 16 patients. 3 and 5 year disease free survival were 52.6%, 48.2% respectively and values of overall survival were 60.0, 43.9% and median survival time was 44.7 months. Treatment response was only a prognostic factor for survival. Laryngeal preservation was observed in twenty-four(60.0%) patients. Conclusion :Initial primary tumor stage was a significant prognostic factor for laryngeal preservation, and response after radiation therapy was a prognostic factor for long-term survival after induction chemotherapy followed by curative radiation therapy for locally advanced hypopharyngeal cancer.

T1N0 성문암의 방사선치료관련 예후 인자 분석

송시열(Si yeol Song),신성수(Seong Soo Shin),김수산(Su Ssan Kim),김종훈(Jong Hoon Kim),조경자(Kyung Ja Cho),최승호(Seung Ho Choi),남순열(Soon Yuhl Nam),김상윤(Sang Yoon Kim),최은경(Eun Kyung Choi),이상욱(Sang wook Lee),안승도(Seung Do 대한두경부종양학회 2003 대한두경부 종양학회지 Vol.19 No.1

Objective: To analyze the treatment-related parameters after the radiotherapy of T1N0 squamous cell carcinoma of the glottic larynx. Materials and Methods: Between October 1989 and August 2000, 54 patients with histologically proven T1N0 squamous cell carcinoma of the glottic larynx who received definitive radiation therapy in Department of Radiation Oncology, Asan Medical Center were analyzed. They were all males with age ranged from 31 to 80 years (median 61 years). 1997 AJCC stages were 31 T1a, 23 T1b. Patients were treated with 4-MV X-rays with a parallel-opposed two-field technique. Ten patients received 66.0-68.4Gy at 1.2Gy per fraction twice daily, 21 patients received 64.8-66.6Gy at 1.8Gy per fraction once daily, and 23 patients received 66.0Gy at 2.0Gy per fraction once daily. Follow-up period was 16-119 months (median 56 months). Results: 5-year overall survival and local control rates for patients with T1 lesions were 87.0% and 88.5%, respectively. 5-year local control with larynx preservation rate was 90.5%. Host and tumor-related prognostic factors including age, stage, anterior commissure involvement and tumor bulk proved not to be significant. Only shorter overall treatment time among treatment-related factors had correlation with imporved local control. Conclusion: Comparable high local control rate with organ preservation was achieved with primary radiation therapy and salvage surgery. Shortening of overall treatment time is related to improved local control rate. To determine the optimal fractionation scheme, randomized trial is mandatory.