SMURF1-mediated ubiquitination of ARHGAP26 promotes ovarian cancer cell invasion and migration

Xuri Chen,Shaoyun Chen,Yao Li,Yanling Gao,Shuying Huang,Hongping Li,Yuanfang Zhu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Rho GTPase-activating protein 26 (ARHGAP26) is a negative regulator of the Rho family that converts the small GTPbinding protein RhoA (GTP-RhoA) to its inactive GDP-bound form and is a putative tumor suppressor gene associated with cell growth and migration. Here, the involvement of ARHGAP26 in ovarian cancer cell proliferation and migration was investigated. In this study, low ARHGAP26 expression was observed in ovarian cancer tissues and was associated with a poor overall survival and higher β-catenin expression in patients with ovarian cancer. A2780 and HEY cells with ARHGAP26 upregulation showed decreased cell proliferation, migration, and invasion, along with decreased GTPRhoA, β-catenin, VEGF, MMP2, and MMP7 expression. ARHGAP26 upregulation in A2780 cells also inhibited lung metastasis in vivo. SKOV3 cells with ARHGAP26 downregulation demonstrated an inverse effect, which was inhibited by ARHGAP26 overexpression or DKK1, an antagonist of the β-catenin pathway. SMURF1, an E3 ubiquitin ligase, interacted with and induced ubiquitination of ARHGAP26. ARHGAP26 upregulation in SKOV3 cells significantly inhibited SMURF1 upregulation-induced cell migration and invasion. Overall, SMURF1-mediated ubiquitination of ARHGAP26 may promote invasion and migration of ovarian cancer cells via the β-catenin pathway.

Molecular Identification of Microbial Community in Chinese Douchi During Post-fermentation Process

Tingtao Chen,Shunqiang Xiong,Shuying Jiang,Mengjuan Wang,Qinglong Wu,Hua Wei 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

To track changes in dynamic microbial communities during post-fermentation process, traditional culture method, and denaturing gradient gel electrophoresis (DGGE) were used to study the number and species of dominant microorganisms in douchi. The result of culturedependent method showed that the microbial number changed slightly since the 4^th day while the DGGE indicated that the really steady-state was achieved from the 10^th day. In addition, Lactococcus lactis subsp.,Staphylococcus lentus, and 2 uncultured bacterium were identified to occupy the dominant positions in bacterial DGGE pattern, and Bacillus thermoamylovorans, Bacillus subtilis, Enterobacter spp., and Absidia corymbifera,Pichia guilliermondii, Pichia farinose were also detected from Bacillus and fungal DGGE patterns, respectively. In conclusion, some pathogenic microorganisms involving in the douchi fermentation had been detected throughout the post-fermentation process, and the combination of culturedependent and –independent method was proved to be effective in profiling microbial diversity.

Novel-integrated process for production of bio-organic fertilizer via swine manure composting

Yan Chen,Xiaoyu Li,Shuying Li,Yongping Xu 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.2

The traditional method of producing bio-organic fertilizer contains two consecutive composting stages which, however, is time-consuming and requires high input of energy and cost. This study attempted to combine two consecutive stages into one single step. Paenibacillus polymyxa, as an antagonistic strain to control anthracnose in Chinese Pakchoi, was used as inoculum to obtain bio-organic fertilizers through two approaches: two consecutive stages (TCS) composting as control and the proposed one step through (OST) composting. The resulting two bio-organic fertilizers produced by TCS and OST were tested for the disease incidence and disease resistant index, and the fertility on growth of Chinese Pakchoi. The results of relative abundance of P. polymyxa showed no significant difference between two kinds of bio-organic fertilizers in phylum, family and genus. The disease incidence of bio-organic fertilizers produced by OST and TCS reduced by 61.40% and 68.42%, respectively, as compared to the control group, where the difference was not significant. The growth promoting effect was remarkable, although the difference in the fertility on the growth was not significant. Compared with the traditional TCS method, the novel OST method could improve efficiency of bio-organic fertilizer production by 42.86% per year according to this study.

Investigation of the super-resolution methods for vision based structural measurement

Zhi Cong Chen,Lijun Wu,Zhouwei Cai,Chenghao Lin,Shuying Cheng,Peijie Lin 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.30 No.3

The machine-vision based structural displacement measurement methods are widely used due to its flexible deployment and non-contact measurement characteristics. The accuracy of vision measurement is directly related to the image resolution. In the field of computer vision, super-resolution reconstruction is an emerging method to improve image resolution. Particularly, the deep-learning based image super-resolution methods have shown great potential for improving image resolution and thus the machine-vision based measurement. In this article, we firstly review the latest progress of several deep learning based super-resolution models, together with the public benchmark datasets and the performance evaluation index. Secondly, we construct a binocular visual measurement platform to measure the distances of the adjacent corners on a chessboard that is universally used as a target when measuring the structure displacement via machine-vision based approaches. And then, several typical deep learning based super resolution algorithms are employed to improve the visual measurement performance. Experimental results show that super-resolution reconstruction technology can improve the accuracy of distance measurement of adjacent corners. According to the experimental results, one can find that the measurement accuracy improvement of the super resolution algorithms is not consistent with the existing quantitative performance evaluation index. Lastly, the current challenges and future trends of super resolution algorithms for visual measurement applications are pointed out.

A novel calculation model for the permeability coefficient of soils conditioned with foam and bentonite slurry

Fanlin Ling,Shuying Wang,Xiangcou Zheng,Jiazheng Zhong,Yujia Chen 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.5

Both foam and bentonite slurry are commonly employed in soil conditioning to prevent water spewing during earth pressure balance (EPB) shield tunnelling. A novel calculation model to estimate the permeability coefficient of soils conditioned with foam and bentonite slurry is developed. In this model, bentonite particles are assumed to adhere to the foam film in a monolayer of bridging particles, and the permeation channels within the conditioned soil are updated using the Kozeny-Carman equation. A series of permeability tests on conditioned soil, varying conditioning parameters and water heads, validate the accuracy of the model. The measured results that the value of permeability coefficient can be well captured by the calculation results, which indicates that the model can accurately predict the permeability of soil conditioning by foam and bentonite slurry. While there is a slight reduction in accuracy for conditioning states with high foam and bentonite slurry injection ratios, the model remains conservative for tunnelling engineering safety due to calculated values consistently exceeding measured ones. Furthermore, the model performs well in cases of foam-conditioned soil from previous studies. Additionally, the model underscores the significant influence of bentonite slurry on the permeability coefficient by altering soil grain gradation.

Facile and highly efficient fabrication of robust Ag nanowire-elastomer composite electrodes with tailored electrical properties

Yang, Ming,Kim, Sang Woo,Zhang, Shuye,Park, Dae Young,Lee, Chang-Woo,Ko, Yong-Ho,Yang, Haifeng,Xiao, Yong,Chen, Gang,Li, Mingyu The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.27

<P>The fabrication of Ag nanowire (AgNW) patterns on a working substrate is a critical step to prepare flexible/stretchable electronics. The conventional method of making AgNW patterns is based on a 2-D mask, which suffers some serious drawbacks, such as low material usage, difficulty in preparing complex patterns, and susceptibility to damaged pattern edges. To overcome these drawbacks, this study proposes the use of a 3-D mask, which when combined with a vacuum filtration system, allowed efficient fabrication of complex AgNW patterns with clear edges. In addition, we controlled the microstructure of the patterned AgNW/polydimethylsiloxane (PDMS) electrodes transferred from the membrane filter, obtaining stretchable electrodes with anisotropic electrical properties. Moreover, we greatly improved the stability and reliability of the patterned electrodes in long-term stretch/release tests by developing a PDMS/AgNW/PDMS sandwich structure. Based on these improvements, robust stretchable electrodes with a stretchability of over 80% uniaxial strain and tunable gauge factors ranging from 0.07 to 520 (a much wider range than previously reported) were fabricated by optimizing the AgNW deposition density and PDMS peel-off direction. Finally, we demonstrated the applicability of the proposed method by fabricating stretchable circuits which were able to either maintain a stable resistance or sensitively monitor various human motions, revealing its tremendous potential for applications in flexible/stretchable electronics.</P>

circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3

Huang Yizhen,Zhang Zhenlei,Wang Jianle,Shen Shuying,Yao Teng,Xu Yining,Chen Zizheng,Fang Bin,Ma Jianjun 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene ( ATP1B3 ) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3 . The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.

Resistant starch (RS), a novel endogenous inert marker for detecting glucose absorption of small intestine with sweeteners administration in mice

Wu Yaran,Cai Lei,Xie Xingzi,Yang Shuying,Shi Qing,Jia Hongzhe,Gu Xuqiang,Deng Jingmin,Shi Mingzhao,Chen Qiuping,Cao Shaoqian,Cai Shuangfeng 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.2

Resistant starch could be degraded by the fermentation of colonic microorganisms in the large intestine of mammals, but not in the small intestine. In this study, we established a novel strategy by using resistant starch as an endogenous marker to determine the glucose absorption of the small intestine of laboratory animals. By optimization of the classical enzymatic method of starch measurement, the demand for the sample weight was reduced by 90%. Moreover, the amount of resistant starch in normal feed was detectable without any extra addition. The value of small intestine glucose absorption of mice was similar when using resistant starch and titanium dioxide as inert markers. The fermentation of resistant starch by intestinal microorganisms in the small intestine was demonstrated not disturbing the detection of glucose absorption significantly. Artificial sweeteners exposed ICR mice showed different glucose absorption which indicated, first, resistant starch can be used as a novel endogenous marker in the small intestine of small animals; second, although glucose tolerance did not change in mice after short-term exposure to artificial sweeteners, there were significant changes in glucose absorption associated with it; third, the short-term exposure resulted in no significant change in glucose tolerance.

The in-situ TEM isothermal aging evolution in a μ-Cu/NiAu/Sn/Cu solder joint for full intermetallic compounds interconnects of flexible electronics

Jinhong Liu,Xinyi Jing,Jieshi Chen,Kyung‑Wook Paik,Peng He,Shuye Zhang 대한금속·재료학회 2024 ELECTRONIC MATERIALS LETTERS Vol.20 No.3

A structure composed of various Cu–Ni–Sn IMCs would develop from severe Joule heat and excessive elemental diff usionunder high-density current in the solder joints of fl exible printed circuit (FPC). Herein, we fi rstly observed the evolution ofa Cu 6 Sn 5 + Cu 3 Sn/(Ni,Cu) 3 Sn 4 hybrid structure in a μ-Cu/NiAu/Sn/Cu solder joint for full intermetallic compounds (IMCs)interconnect of fl exible electronics under isothermal aging condition by in-situ TEM. The joint was divided into two regions,the IMC type on the right region remained unchanged with dwell time prolonging, while the ratio of Cu 3 Sn on the left regionat various dwell times fi tted the JMAK model when the kinetic parameter n picked 1.5, indicating that grain boundary diff usionwas the predominant mechanism for transporting Cu atoms. The nucleation and growth of Cu 3 Sn grains were fi nishedin the Cu 6 Sn 5 layer. The nucleation of a Cu 3 Sn grain with a spherical cap shape was fi rstly captured by HRTEM, and Cu 3 Sngrains underwent a transformation from columnar to equiaxed when the dwell time was increased, making the morphologyof Cu 3 Sn grains in a μ-Cu/NiAu/Sn/Cu solder joint signifi cantly diff erent from the situation in larger solder joints. This studyis expected to provide an in-depth study of the microstructural evolution of micro Cu/NiAu/Sn/Cu solder joints under agingcondition and thereby expand their application in the microelectronic industry.

Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration

Wang Jianle,Xia Dongdong,Lin Yan,Xu Wenbin,Wu Yaosen,Chen Jiaoxiang,Chu Junjie,Shen Panyang,Weng Sheji,Wang Xiangyang,Shen Lifeng,Fan Shunwu,Shen Shuying 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Low back pain, triggered by intervertebral disc degeneration (IVDD), is one of the most common causes of disability and financial expenditure worldwide. However, except for surgical interventions, effective medical treatment to prevent the progression of IVDD is lacking. This study aimed to investigate the effects of circKIF18A, a novel circRNA, on IVDD progression and to explore its underlying mechanism in IVDD. In this study, we found that oxidative stress was positively correlated with nucleus pulposus cell (NPC) senescence in IVDD and that circKIF18A was downregulated in IVDD and attenuated senescent phenotypes such as cell cycle arrest and extracellular matrix degradation in NPCs. Mechanistically, circKIF18A competitively suppressed ubiquitin-mediated proteasomal degradation of MCM7, and the protective effects of circKIF18A on NPCs were partially mediated by MCM7 under oxidative stress. Intradiscal injection of adenoviral circKIF18A ameliorated IVDD in a rat model. This study revealed that circKIF18A regulates NPC degeneration by stabilizing MCM7 and identified a novel signaling pathway, the circKIF18A-MCM7 axis, for anti-senescence molecular therapy in IVDD.