circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3

Huang Yizhen,Zhang Zhenlei,Wang Jianle,Shen Shuying,Yao Teng,Xu Yining,Chen Zizheng,Fang Bin,Ma Jianjun 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene ( ATP1B3 ) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3 . The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.

A Study on the Optimization of Anisotropic Conductive Films for Sn-3Ag-0.5Cu-Based Flex-on-Board Application at a 250 °C Bonding Temperature

Zhang, Shuye,Yang, Ming,Wu, Yang,Du, Jikun,Lin, Tiesong,He, Peng,Huang, Mingliang,Paik, Kyung-Wook IEEE 2018 IEEE transactions on components, packaging, and ma Vol.8 No.3

<P>Although cationic epoxy was optimized for low-melting SnBi58 solder ACF joints with the lowest coefficient of thermal expansion (CTE) in terms of reliability, cationic epoxy also showed a faster curing property than any other types of adhesives. In fact, solder joint shapes at 250 °C bonding are very different from those shapes at 200 °C bonding. In this paper, four adhesive film types were investigated in terms of Sn-3Ag-0.5Cu solder ACF joint shapes on the electrical performances and reliability in a pressure cooker test (PCT). Thermal stability of adhesive films was tested to be first. Resin curing speeds were measured in a 250 °C isothermal mode differential scanning calorimetry, resin viscosities were checked by a parallel-plate rheometer, and adhesive thermomechanical properties, such as modulus and CTE, were characterized. Then, four different types of ACF resins containing the same weight percentages of Sn-3Ag-0.5Cu solders were assembled by the same thermocompression bonding parameter (250 °C 10s 2MPa on bump) on a 500- <TEX>$\mu \text{m}$</TEX>-pitch flex-on-board (FOB) application, and different solder joint morphologies were verified. Various bonded solder ACFs joints were compared in terms of solder wetting areas, electrical performances by a four-point-probe method, and the reliability of PCT (121 °C 100% humidity 2atm) for 120 h. This paper aims at optimizing the best adhesive film candidate for SAC305 solder ACF joints of FOB application.</P>

Effect of Grain Gradation on the Permeability Characteristics of Coarse-grained Soil Conditioned with Foam for EPB Shield Tunneling

Shuo Huang,Shuying Wang,Changjie Xu,Yufeng Shi,Fei Ye 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.11

To avoid water spewing out of the screw conveyor, foam is often used to reduce the permeability coefficient of the soil during earth pressure balance (EPB) shield tunneling. Whether foam can effectively reduce the permeability or not strongly depends on soil gradation characteristics. A series of permeability tests were carried out on foam-conditioned soil with changing effective grain size (d10), curvature coefficient (Cc), and uniformity coefficient (Cu), further, three characteristic times were defined to evaluate the permeation safety of the conditioned soil, including critical time (tc), flow rate safety time (ta) and flow quantity safety time (ts). The test results show that with an increase in d10, the initial permeability coefficient of the conditioned soil increased greatly, however, it only changed slightly with increases in Cc and Cu. In addition, permeability coefficient was still much lower than that of the unconditioned soil, even though entered the slow growth period for a long time. It is shown that as the Cc and Cu increased, the ta and ts changed slightly, however, as the d10 increased, the ta and ts decreased significantly. The results indicate that d10 can greatly affect the permeability characteristics of conditioned soil, more specifically, the initial permeability coefficient and the permeation safety. By contrast, Cc and Cu have less effects. In addition, it is not reasonable to consider that the permeability coefficient of the conditioned soil will reach or be close to that of unconditioned soil when most, or all, of foam in the foam-conditioned soil dissipates.

Unified Error Probability Analysis for Error Correcting Codes with Different Decoding Algorithms

Liyun Dai,Wenyuan Rao,Shuying Huang,Yingjun Tang,Yong Yang,Jiancheng Sun 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.2

The error rate of error correcting codes with soft-decision-decoding rarely has a closed-form expression. Bounding techniques are widely used to evaluate the performance of maximum-likelihood decoding algorithm. But the existing bounds are not tight enough especially for a low signal-to-noise ratios region and become looser when a suboptimum decoding algorithm is used. The radius of decision region is applied to evaluate the word error rate (WER) of error correcting codes with different decoding algorithms. Simulation results show that this method can effectively evaluate the WER of different decoders with 0.05dB maximum error.

Magnetostrictive and Magnetic Properties of Tb<SUB>0.29</SUB>Dy<SUB>0.48</SUB>Ho<SUB>0.23</SUB>Fe<SUB>1.9</SUB> Fiber/Epoxy Composites

Ran Zhao,Bowen Wang,Shuying Cao,Wenmei Huang,Quanguo Lu,Jianwu Yan 한국자기학회 2018 Journal of Magnetics Vol.23 No.2

In this paper, we fabricated novel magnetostrictive composites by embedding <110>-oriented Tb-Dy-Ho-Fe fibers in an epoxy matrix. The magnetostrictive and magnetic properties (magnetostriction, magnetization, piezomagnetic coefficient and relative permeability) of the proposed composites were measured, analyzed, and compared to those of Tb0.29Dy0.48Ho0.23Fe1.9 alloy and Tb0.3Dy0.7Fe₂ continuous-fiber/epoxy composites. Tb0.29Dy0.48Ho0.23Fe1.9 continuous-fiber/epoxy composites have a saturation magnetostriction (λs) of 840 ppm and saturation magnetization (Ms) of 0.75 T. Their piezomagnetic coefficient exhibits a maximum value (8.2 μm/kA) at 19 kA/m. These proposed composites exhibit a large magnetostriction in high magnetic fields (> 400 kA/m) and a large relative permeability in low magnetic fields (< 100 kA/m). This result indicates that the given composites perform better than the Tb0.29Dy0.48Ho0.23Fe1.9 alloy and Tb0.3Dy0.7Fe₂ fiber/epoxy composites. Thus, the composites with characteristics of high sensitivity and large magnetostriction can be used in the field of ultrasonic sensing.

A Novel Automatic Block-based Multi-focus Image Fusion via Genetic Algorithm

( Yong Yang ),( Wenjuan Zheng ),( Shuying Huang ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.7

The key issue of block-based multi-focus image fusion is to determine the size of the sub-block because different sizes of the sub-block will lead to different fusion effects. To solve this problem, this paper presents a novel genetic algorithm (GA) based multi-focus image fusion method, in which the block size can be automatically found. In our method, the Sum-modified-Laplacian (SML) is selected as an evaluation criterion to measure the clarity of the image sub-block, and the edge information retention is employed to calculate the fitness of each individual. Then, through the selection, crossover and mutation procedures of the GA, we can obtain the optimal solution for the sub-block, which is finally used to fuse the images. Experimental results show that the proposed method outperforms the traditional methods, including the average, gradient pyramid, discrete wavelet transform (DWT), shift invariant DWT (SIDWT) and two existing GA-based methods in terms of both the visual subjective evaluation and the objective evaluation.

Medical Image Fusion via an Effective Wavelet-Based Approach

Yang, Yong,Park, Dong Sun,Huang, Shuying,Rao, Nini Hindawi Publishing Corporation 2010 EURASIP journal on advances in signal processing Vol.2010 No.1

<P>A novel wavelet-based approach for medical image fusion is presented, which is developed by taking into not only account the characteristics of human visual system (HVS) but also the physical meaning of the wavelet coefficients. After the medical images to be fused are decomposed by the wavelet transform, different-fusion schemes for combining the coefficients are proposed: coefficients in low-frequency band are selected with a visibility-based scheme, and coefficients in high-frequency bands are selected with a variance based method. To overcome the presence of noise and guarantee the homogeneity of the fused image, all the coefficients are subsequently performed by a window-based consistency verification process. The fused image is finally constructed by the inverse wavelet transform with all composite coefficients. To quantitatively evaluate and prove the performance of the proposed method, series of experiments and comparisons with some existing fusion methods are carried out in the paper. Experimental results on simulated and real medical images indicate that the proposed method is effective and can get satisfactory fusion results.</P>

An Improved H.264 Encoded Algorithm Based on Weber-Fechner Law

Yanhui Xia,Baisheng Nie,Zhengyou Wang,Liying Li,Jianhua Ming,Zheng Wan,Shuying Huang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.9

With the study of human visual system (HVS), people find that the human eye shows different degree of sensitivity to different light signals. A higher background brightness region of the human eye for the distortion, the degree of sensitivity will be greatly reduced. The human eye’s sensitivity will be greatly reduced to the distortion of a higher brightness background region. This finding is discribed in Weber-Fechner law. Therefore, the encoder can be improved by employing this characteristic of human visual system. In this paper, the authors use this characteristic to improve the H.264 video coding algorithm. In order to improve the compression efficiency of encoder, give the brightness of different regions with different levels of quantification by adjusting the quantization step size (QP), without affecting the subjective quality of video. Experimental results show that the output bit rate decrease up to 10% -20% by using this improved algorithm in H.264 reference encoder, and the subjective quality of decoded image by using this improved algorithm is as good as the standard H.264 encoders.

SMURF1-mediated ubiquitination of ARHGAP26 promotes ovarian cancer cell invasion and migration

Xuri Chen,Shaoyun Chen,Yao Li,Yanling Gao,Shuying Huang,Hongping Li,Yuanfang Zhu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Rho GTPase-activating protein 26 (ARHGAP26) is a negative regulator of the Rho family that converts the small GTPbinding protein RhoA (GTP-RhoA) to its inactive GDP-bound form and is a putative tumor suppressor gene associated with cell growth and migration. Here, the involvement of ARHGAP26 in ovarian cancer cell proliferation and migration was investigated. In this study, low ARHGAP26 expression was observed in ovarian cancer tissues and was associated with a poor overall survival and higher β-catenin expression in patients with ovarian cancer. A2780 and HEY cells with ARHGAP26 upregulation showed decreased cell proliferation, migration, and invasion, along with decreased GTPRhoA, β-catenin, VEGF, MMP2, and MMP7 expression. ARHGAP26 upregulation in A2780 cells also inhibited lung metastasis in vivo. SKOV3 cells with ARHGAP26 downregulation demonstrated an inverse effect, which was inhibited by ARHGAP26 overexpression or DKK1, an antagonist of the β-catenin pathway. SMURF1, an E3 ubiquitin ligase, interacted with and induced ubiquitination of ARHGAP26. ARHGAP26 upregulation in SKOV3 cells significantly inhibited SMURF1 upregulation-induced cell migration and invasion. Overall, SMURF1-mediated ubiquitination of ARHGAP26 may promote invasion and migration of ovarian cancer cells via the β-catenin pathway.