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        Identification of a novel cytoplasmic male sterile line M2BS induced by partial-length HcPDIL5-2a transformation in rice (Oryza sativa L.)

        Danfeng Tang,Peng Chen,Gang Jin,Min Li,Cancan Wang,Zhenlan Bin,Jinghua Qian,Zizheng Wang,Ruiyang Zhou 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

        A cytoplasmic male sterile line (designated as M2BS) was obtained from an indica rice maintainer M2B induced by partial-length HcPDIL5-2a (Hibiscus cannabinus protein disulfide isomerase-like) transformation. The anther of M2BS was short, slender, hygrophanous, and fissured. I2-KI staining method showed that there was typical and spherical abortion in pollen grains. M2BS was found abortive at middle and late stage of monocyte by the modified carbol fuchsin stained observation and paraffin section observation. The tapetum was observed pre-degenerated in M2BS. Hereditary analysis indicated that the male sterility of M2BS was a maternally inherited inability after six backcross generations with M2B and the combinations of M2BS hybridized with other two male fertile materials. The M2BS could be affirmed a cytoplasmic male sterile (CMS) type. Moreover, it was a transgenic plant confirmed by PCR, Southern blot and RT-PCR detection. M2BS could be distinguished from M2B and its CMS line M2A by RFLP analysis. The overall mitochondrial genome sequencing results showed, that in M2BS, the main differences of mitochondrial gene sequence were located in nad4, nad5, nad7, orf194 and intergenic region, relatively to those of M2A. The obtained results indicate that M2BS is a novel cytoplasmic male sterile line.

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        An Optical Graphene-silicon Resonator Phase Shifter Suitable for Universal Linear Circuits

        Changling Liu,Jianping Wang,Hongyao Chen,Zizheng Li 한국광학회 2022 Current Optics and Photonics Vol.6 No.1

        This paper describes the construction of a phase shifter with low loss and small volume. To construct it, we use the two graphene layers that are separated by a hexagonal boron nitride (hBN) and embedded in a silicon waveguide. The refractive index of the waveguide is adjusted by applying a bias voltage to the graphene sheet to create an optical phase shift. This waveguide is a compact device that only has a radius of 5 μm. It has a phase shift of 6π. In addition, the extinction ratio (ER) is 11.6 dB and the inser-tion loss (IL) is 0.031 dB. Due to its unique characteristics, this device has great potential in silicon onchip optical interconnection and all-optical multiple-input multiple-output processing.

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        circSPG21 protects against intervertebral disc disease by targeting miR-1197/ATP1B3

        Huang Yizhen,Zhang Zhenlei,Wang Jianle,Shen Shuying,Yao Teng,Xu Yining,Chen Zizheng,Fang Bin,Ma Jianjun 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

        The abnormal expression of circular RNAs (circRNAs) is associated with numerous human diseases. This study investigated the mechanism by which circRNA acts as competitive endogenous RNA in the regulation of degenerative intervertebral disc disease (IVDD). Decreased expression of circSPG21 was detected in degenerated nucleus pulposus cells (NPCs), the function of circSPG21 in NPCs was explored and verified, and the downstream target of circSPG21 was investigated. The interaction between circSPG21 and miR-1197 and its target gene ( ATP1B3 ) was studied by online database prediction and molecular biological verification. Finally, the circSPG21/miR-1197/ATP1B3 axis was verified in the mouse tail-looping model. The expression of circSPG21 in the nucleus pulposus in IVDD was directly related to an imbalance of anabolic and catabolic factors, which affected cell senescence. circSPG21 was found to play a role in human NPCs by acting as a sponge of miR-1197 and thereby affecting ATP1B3 . The regulation of circSPG21 provides a potentially effective therapeutic strategy for IVDD.

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