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Mutations in gyrA and gyrB Among Drug Resistant Mycobacterium Tuberculosis in Korea
( Seungmo Kim ),( Ryeun Heo ),( Hee Joo Lee ),( Cheon-Tae Kim ),( Hee-Jin Kim ),( Jong-Wook Hong ),( Soul-hee Kim ),( Tae-Shik Park ),( Dea-Seon Han ),( Hyeon-Su Kim ),( Jong-Myun Song ),( Mi-so Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0
Background Fluoroquinolones have been recognized as important anti-TB agents based on new tuberculosis treatment guidelines. In this study we have evaluated gyrA and gyrB how to relate with MIC based on 7H9 and phenotypic DST Results based on Löwenstein-Jensen (L-J) medium. Method Since 13th July 2020, 108 cases have analyzed gyrA and gyrB genes for fluoroquinolones. We have tested bedaquiline (0.03125~4 μg/mL), delamanid (0.00625~0.8 μg/mL), moxifloxacin (0.0625~8.0 μg/mL), levofloxacin (0.0625~8.0 μg/mL), linezolid (0.0625~8.0 μg/mL) using 7H9 broth. In Löwenstein-Jensen (L-J)medium, low concentration of moxifloxacin was 1.0 μg/mL and high concentration of moxifloxacin was 2.0 μg/mL, And that of levofloxacin and linezolid was 2.0μg/mL. Results All of mono-INH resistant cases analyzed wild type in gyrA and gyrB genes. There were 7 mutant type analyzed in gyrA and 1 mutant type in gyrB gene among 54 RIF resistant and MDR cases. All of 8 cases what we analyzed mutant type in gyrA and gyrB genes was interpreted resistant in phenotypic DST using L-J medium. Additionally all of 52 cases interpreted susceptible in phenotypic DST using L-J medium was wild type in gyrA and gyrB genes. The distribution of MICs for moxifloxacin what analyzed mutant type in gyrA gene was middle and high level (1.0 ~ 4.0 μg/mL). And The MIC for moxifloxacin what analyzed mutant type in gyrB gene was middle level (0.5 μg/mL). The distribution of MICs for levofloxacin what analyzed mutant type in gyrA gene was high level (4.0 ~ 8.0 μg/mL). And The MIC for levofloxacin what analyzed mutant type in gyrB gene was middle level (1.0 μg/mL). Conclusions We found 8 mutant type cases in gyrA and gyrB genes. Most of mutant type (75%) was gyrA_D94G(GAC/GGC). We observed gyrA and gyrB genes were associated with MICs based on 7H9 and phenotypic DST Results based on L-J medium.
적외선 가연성가스 센서의 입출력 특성 및 캘리브레이션 연구
김승모(Seungmo Kim),박규태(Gyoutae Park),김병덕(Byuongduk Kim),조영도(Youngdo Jo),김희식(Hiesik Kim) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.11
In this paper, we conducted a study of the calibration to grasp the input and output characteristics of the two types of gas sensors to be used in a flammable gas detector. In fact, when developing gas detectors, there are many difficulties, which the specifications listed in the sensor and the measured data values are not matched each other. It is necessary validation and calibration of specifications when sensors are used practically. In this study, the output voltages were measured by inputs of 8 kinds of standard gases into the two types of infrared gas sensor. The linear regression function derived by using least square estimation to represent the output voltages. When inverting the derived linear regression function and actually measuring its approximation equation, errors occur due to pressure of gas injection, temperature, humidity and nonlinearity of circuits. In order to reduce these errors and optimize responses of sensor"s inputs and outputs, we adjusted the slope and offset of the designed equation. Also, it is confirmed through experiment that the output according to the input is linear other than before.
Mutations in gyrA and gyrB among Drug Resistant Mycobacterium Tuberculosis in Korea
( Hee Joo Lee ),( Hwi-Jun Kim ),( Ryeun Heo ),( Cheon-Tae Kim ),( Hee-Jin Kim ),( Jeong-hui Gwon ),( Gicheon Bae ),( Sumi Kang ),( Soul-hee Kim ),( Seungmo Kim ),( Eunseon Kim ),( Arim Song ),( Dea-Se 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0
Purpose In 2020 KATRD, we analyzed 65 isolates to see how gyrA and gyrB are associated with 7H9 broth microdilution method (BMD) and Lowenstein-Jensen (L-J) drug susceptibility test (DST) because fluoroquinolones (FLQ) have been recognized as important anti-TB agents. In this study, the aim is to evaluate the correlation between gyrA and gyrB mutations and resistance to FLQ with BMD and L-J DST as a follow up of the previous study. Method Since 2020, we have analyzed 304 INH or RIF mono resistant cases by molecular DST using sequencing for gyrA and gyrB genes of FLQ. MICs were measured by BMD for moxifloxacin (MFX, 0.0625~8.0 μg/mL) and levofloxacin (LFX, 0.0625~8.0 μg/ mL). In L-J DST, concentration of MFX was 1.0 μg/mL, 2.0 μg/mL and LFX was 2.0 μg/mL. Results In gyrA and gyrB sequencing, 270 strains (88.81%) were wild type (WT), 34 strains (11.18%) were mutant type (MT). Among 29 gyrA MT strains, 13 isolates (44.82%) had mutation at D94G, 7 isolates (24.14%) at A90V and 5 isolates (17.24%) at D94A. Among 5 gyrB MT strains, 2 isolates (40%) had D500N mutation. In L-J DST, 100% of gyrA MT strains were resistant to MFX. On the other hand gyrB MT strains, 60% to MFX and 40% to LFX were resistant. In BMD, 93.10% of gyrA MT strains ranged 0.5 ~ 4.0 μg/mL and 60% of gyrB MT strains ranged 0.5 ~ 4.0 μg/mL to MFX. Meanwhile 96.55% of gyrA MT strains ranged 1.0 ~ 8.0 μg/mL and 80% of gyrB MT strains ranged 1.0 ~ 8.0 μg/mL to LFX. Conclusions Most of the mutant isolates had mutations in gyrA and most of mutant type (38.23%) was gyrA_D94G (GAC/GGC). In this study we observed gyrA genes were associated with higher MICs based on 7H9 and L-J DST than gyrB genes. # No.2021R1F1A1061358
Seungmo Lim,Jeong-Seon Kim,Moon Nam,Ran Hee Yoo,Fumei Zhao,Sang-Mok Kim,Yong-Gil Shin,Su-Heon Lee,Jae Sun Moon 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
In this study, we constructed viral vector for soybean by using Soybean yellow common mosaic virus (SYCMV) infecting both Glycine max and Glycine soja. SYCMV-derived viral vector was tested to use as Virus-induced gene silencing (VIGS) vector for functional analysis of soybean genes and as protein expression vector for foreign protein expression. In vitro transcript with 5’ cap analog m7GpppG from a full-length infectious vector of SYCMV driven by T7 promoter was inoculated to soybean to test infectivity of the clone (pSYCMVT7-full). 5’-capped transcript was able to infect soybean plants. The symptoms observed in soybean plants infected by either the vector or the sap from SYCMV-infected leaves were indistinguishable, suggesting that the vector had an equal biological activity shown by virus itself. To further utilize the vector, an additional DNA-based vector was constructed. The full-length cDNA was inserted into a binary vector flanked by CaMV 35S promoter and the nopaline synthase terminator (pSYCMV35S-full). To test if the vector infects soybean and subsequently induces gene silencing, we prepared two constructs containing fragments of Phytoene desaturase (PDS) gene (pSYCMV35S-PDS1) and small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS) gene (pSYCMV35S-rbcS2) from soybean plant. Plants infiltrated with the constructs through Agrobacterium-mediated method showed distinct symptoms such as photobleaching in plants infiltrated with pSYCMV-PDS1 and pale green or yellowing in plants infiltrated with pSYCMV-rbcS2. In addition, down-regulations of mRNA levels of two genes were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To test if the vector can be used for foreign protein expression in soybean plants, we prepared a construct encoding amino acids 135-160 of VP1 FMDV serotype O1 Campos (O1C) (pSYCMV35S-FMDV). Plants infiltrated with the construct through Agrobacterium-mediated method showed that soybean plant infiltrated with pSYCMV35S-FMDV only was detected by Western blotting using O1C antibody. These results support that SYCMV-derived viral vector can be used as VIGS vector or protein expression vector in soybean plants.
The Effects of Coaching Styles on Artistic Swimmers’ Intention to Continue Athletic Career
Seungmo Kim,Tsz Ching Pang,박상현 상명대학교 글로벌문화예술교육연구소 2019 Research in Dance and Physical Activity Vol.3 No.2
The main purpose of the current study was to analyze the effects of coaches’ leadership styles on athletes’ intention to continue their athletic career in the sport of artistic swimming. The population of this study was all athletic swimmers at the elite level. A sample of 196 artistic swimmers from 23 countries participated in a questionnaire pertaining to autocratic coaching, democratic coaching style, and intention to continue athletic career. Frequent analysis and multiple regression analyses were conducted to examine the relationships between two coaching styles and the outcome variable. The results revealed that democratic coaching style had a positive influence on athletes’ intention to continue, whereas autocratic coaching style had negative influence on athletes’ intention to continue. The results suggest that democratic coaching style could be more effective than autocratic coaching style to induce a long-term participation in the sport.