Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Epstein-Barr 바이러스 형질전환법을 이용한 파상풍 톡소이드에 대한 사람 단세포군 항체의 생산

유승민,조정제,호순태,하윤문 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

For production of tetanus toxoid(TT) specific human monoclonal antibodies, anti-TT antibody secreting peripheral B lymphocytes were separated by rosetting with TT-coated SRBC. And, B lymphoblastoid cell line(BLCL) was established by Epstein-Barr Virus(EBV) transformation methods. Stable BLCLs were established after three times of cloning by limiting dilution. To establish the maximal antibody producing condition, several environmental factors were tested. The optimal condition for maximum antibody prodiction was 4 day culture of 1 x 105cells/ml concentration in RPMI 1640 complete culture media supplemented with 20% fetal calf serum, and the maximum concentration of secreted antibody was about 900ng/ml. The antibody production of BLCL was decreased during long-term culture after establishment of cell line, but antibody production was maintained by repeated cloning by limiting dilution.

포도종실 추출물이 Methylcellulose 필름 성질에 미치는 영향

유승모,박준서,정하열,심재용,김병호,박장우 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

The film with methylcellulose(MC) and grape seed extract(GSE) were prepared by casting their aqueous solution at 80℃ and drying at 23℃ for 48 hrs. Effect of grape seed extract(GSE) on the barrier permeabilities and the thermomechanical property of methylcellulose(MC) film was studied. The barrier permeabilities and the thermomechanical property of MC film containing various contents of GSE were evaluated in terms of water vapor, oxygen permeabilities, and dynamic mechanical analysis(DMA). The rate of water vapor permeability of MC film decreased with GSE contnets in the MC films. Also, the rate of oxygen permeability also decreased with GSE contents indicating higher oxygen barrier property of MC film. The results showed that GSE had a good miscibility with MC and acted as a plasticizer for MC film resuling in lowering the glass transition temperature of the film.

정기적인 수영훈련이 어린이들의 신체작업능력 및 심폐기능에 미치는 영향

유승희,심성섭,박찬호 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The following results have been won after the periodical experiment by way of multistage loading by using the cycle ergometer for the concrete items about Physical Fitness, Physical work Capacity and cardiovascular function to investigate the effects of the swimming to the boys and girls each numbered 20(in the 4th, 5th, and 6th grade in the elementary School) who have been the objects of the experiments and have been participating in the swimming training periodically and other boys and girls each numbered 20 who have not been participating and have not experienced such physical exercises. 1.In the comparison about the physicla Fitness, In case of the boys participating group showed higher than the ordinary group by Back strength 1.69kg, grip strength 2.43kg, Arm Flexion strength 0.75kg, Leg Extension strength 2.4kg and Trunk Flexion 2.11cm while the ordinary group showed higher in the jump step test by 1.7beats than the participating group but not so significant difference. 2.In the comparison about the physical Fitness, in case of the girls participating group showed higher than the ordinary group by Back strength 0.52kg, grip strenght 2.14kg, Arm Flexition strength 2.5kg, each Leg Extension strength 8.88kg, Trunk Flexion 1.34cm and 1.3beats in the Jump step test and the Leg Extension strength has been higher( p<.05) by notable difference. 3.In the Physical work capacity, the participating group showed higher than the ordinary group by 0.38 minutes in the boys and 2.01minutes in the girls group the not able difference appeared(p<.05) and the maximum exercise hour showed higher in the participating group by 1.03 minutes in the boys(p <.05) and in the girls by 2.24 minutes(p<.01). 4.In the comparison about the cardiovascular function participating group showed lower by respiration of single ventilation and Heart rate in one time also in the respiration rate, Q2 pulse, Oxygen uptake, ventilation of carbon dioxide, oxygen uptake per weight, rate of gas exchange the participating group showed lower but not notable difference in the boys group. In the maximum value the participating group showed higher by the notable differences(p<.01) in ventilation of carbon dioxide and oxygen uptake per weight but in one time ventilation it showed lower. And in the oxygen uptake per weight, oxygen uptake and rate of gas exchange there appeared notable differences(p<.05) In the participating group by higher and there have been no notable differences in the respiration rate and Heart rate. 5.In the comparison about the cardiovascular function, in case of girls, when they are in the stable state, the Heart rate and rate of gas exchange showed little lower in the participating group but in the respiration of one time ventilation, respiration rate, Q2 pulse, oxygen uptake, ventilation of carbon dioxide and oxygen uptake per weight they showed higher but no notable differences. In the maximum value there showed higher by the notable differences(p<.05) in the respiration of one time ventilation of carbon dioxide, O2 pulse of the participating group and in the respiration rate and rate of gas exchange it showed little higher and in the Heart rate it showed little lower but no notable difference.

최대운동부하시 단련자와 비단련자의 호흡순환기능 비교연구

유승희,김복현,송종국,윤형기,박찬호 경희대학교 체육과학연구소 1996 體育學論文集 Vol.24 No.-

We studied the differences of cardiorespiratory function of the untrained group and the trained group. The findings are following: 1.In ventilation, trained group was higher than untrained group. In addition, there was significant difference(p〈.001) at 60% and 85% exercise levels and All-out time. 2.In respiratory rate, tranined group was higher than untrained group. In addition, there was significant difference(p〈.01)at 85% exercise level and All-out time. 3.In respiratory gas exchange, trained group was lower than untrained group. In addition, there was significant difference(p〈.01) at All-out time. 4.In oxygen intake per weight, trained group was higher than untrained group. In addition, there was significant difference(p〈.001) at 40%, 60%, and 85% excercise levels and All-out time. 5.In heart rate, trained group was lower than untrained group. In addition, there was remarkable difference(p〈.0901) at rest.

치수/치근단 질환에 이환된 영구치의 치수 조직 재생과 치근 형성

유연지,백승호,손호현 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.4

최근 치수 질환 또는 치근단 질환을 가진 미성숙 영구치에 대한 보존적 치료의 방법으로 여러가지 근관 내 소독 약제를 이용하에 증상 개선은 물론 치근의 성장 및 치수의 재생이 이루어진 증례들이 보고되고 있다. 그 기전에 대해서는 아직 명확하게 밝혀지지는 않았지만 여러가지 줄기 세포 또는 미분화 간엽 세포들이 관여하는 것으로 생각되며, 실제로 재생된 조직에서는 대부분백악질양또는골양물질의 침착이 관찰되고 있다. 이 새롭고보존적인 치료접근 방법은 다능성 줄기 세포와 다양한조직 공학 기술에 대한 연구와 더불어, 재생적 근관 치료에 더 밝은 비전을 제시하고 있다. Numerous cases about additional growth of roots or pulp tissue regeneration by using various intracanal medicaments in immature permanent teeth with periapical or pulpal disease have been reported. The underlying mechanism has not been clearly delineated. but it has been widely accepted that undifferentiated mesenchymal cells and stem cells are involved. Moreover, the growth and deposition of osteoid or cementoid tissues have been observed in regenerated pulp and roots. This new and non-invasive treatment has brightened the future of endodontics. and enlarged the vision of regenerative root canal treatment with multi-potent stem cells and various tissue engineering techniques.

중증 원형 탈모증 치료법으로서 경구 프로드니솔론과 국소제의 병용 효과

유형철,이지범,원영호,이승철 全南大醫大 2000 전남의대학술지 Vol.36 No.1

최대근력부하와 폭발적 근력부하에 의한 신경-근피로와 그 회복과정 비교 분석

유관호,이무연,이승훈,김민섭,육조영,임정일,임완기,김대경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The result which was gained from this study is as follows. From the conclusion of this study it was confirmed that resistant motion at high level strength brings about rise in density of lactate in blood at the same time decline of muscular power and generates acute fatigue of nerve-muscle accompanied by decline in electrical activity of muscle. On the other hand explosive loading produced fatigue at body center and decline in nervine-muscular activity particularly for men, In case of women explosive load did not produce fatigue in the same level as men and they were also quicker in recovery after exercise. Based on 2 types of loading method used in this study there was clearly difference in the process of restoration from resistant motion. For greater decline in muscular power and electromyogram activity more longer period of recovery was require.

8주간의 수영훈련이 호흡순환기능에 미치는 영향

유승희,김복현,심성섭,박찬호 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

In this study, ten students of department of physical education in K unversity participated in 8-week-swimming training. With the figures of respiration and circulation shown before the training and after the trainging, the conclusion came out as follows after comparison and analysis of the changes in the stability and the maximum figures. 1)The respiration of one time ventilation(TVE, ㎖) Looking info the quantity of one lime ventilation In the rest, it came out 946.47㎖, meanwhile, after the training, it fell down to 878.17㎖, which is lower by 68.39㎖ and the the percentage of decrease is 7.78%. But as for the maximum figures, in came out 3117.70㎖, while after the training, it was 3219.10㎖ and that's higher by 101.4㎖ and the percentage of increase is 3.15%. 2)The oxygen uptake(VO2, ㎖/min) In the rest, the quantity of Q2 taken into the lung was 540.83m7/min before the training, but after the trainging, it showed 405.63㎖/min, which was lower by 135.20㎖/min, and the percentage of decrease is 33.33%. But as for the maximum figures, it was 3701.60㎖/min before the training, while after the training, it was 3885.30㎖/min, which Is higher by 183.7㎖/min and the percentage of· increase is 4.73%. 3)The rate of gas exchange(R)In the rest, the rate of gas exchange showed no big difference between before (1.03) and after (1.03) the training. But in the maximum figures, it was 1.42 before the training, while it was 1.45, which was higher by 0.03 and the rate of increase is 2.07%. 4)The oxygen uptake per weight(VO2/W, ㎖/kg/min)In the rest, the quantity of O2 per weight was 8.05㎖/kg/min before the training. But after the training, it was 5.99㎖/kg/min, which was lower by 2.06㎖/kg/min, and the rate of decrease is 34.39%. But in the maximum figures, it was 53.64㎖/kg/min before the trainging, while it was 58.80㎖/kg/min, which was higher by 5.16 and the rate of increase is 8.78%. 5)Heart rate(HR, beats/min)Looking Into the heart rate in the rest, it was 74.24beats/min before the training, but after the training, it was 70.84beats/min, which was lower by 3.4beats/min and the rate of decrease is 4.79%. But in the maximum figures, before the training, it was 187.25beats/min while it came out 194.69beats/min, which was higher by 7.44beats/min and the rate increase was 3.82%. 6)O2 pluse(VO2/HR, ㎖/beats)In the rest figures came out 6.10 before the training, while after the training, it was 5.72㎖/beats which was lower by 0.38 and the rate of decrease was 6.64%. But in the maximum figures, it was 19.78㎖/beats before the training, but after the training, while after the training, it was 22.54me/beats, which was higher by 2.76 and rate of increase is 12.24%.

관상동맥 질환자를 위한 cycle ergometer exercise와 treadmill exercise의 적정 운동강도

유승희,장완성,박찬호,김승환,노호성 경희대학교 체육과학연구소 2000 體育學論文集 Vol.28 No.-

The purpose of this study was to determine heart rate (HR), ratings of perceived exertion (RPE) and oxygen uptake (Vo2) at the anaerobic threshold (AT) in two different exercise modalities, cycle ergometer and treadmil exercise (running). Subjects were 13 women patients with coronary heart disease. All sub-maximal and maximal Vo2 values but Vo2 at the AT for women were significantly higher in treadmill exercise than cycle ergometer. Submaximal and maximal HR values were not significantly different between the two exercise modalities. However, mean RPE values at the AT were significantly higher in cycle ergometer than treadmill exercise. We suggest that appropriate HR values in exercise training should be the same in cycling and treadmill exercise. while RPE values should be 1 or 2 higher in cycling than running.