근린생활권의 물리적 환경이 신체활동 목적의 공원이용에 미치는 영향

이슬기(Lee, Seul-Gi),이우성(Lee, Woo-Sung),백수경(Baek, Su-Kyeong),정성관(Jung, Sung-Gwan),박경훈(Park, Kyung-Hun) 대한국토·도시계획학회 2013 國土計劃 Vol.48 No.7

Urban green space is a valuable community asset that can play an important role in promoting physical activity for health. Therefore, this study examined an association between the characteristics of neighborhood-based physical environment on park usage and physical activity. Geographic information system(GIS) was used to construct spatial data regarding the physical environments across Changwon-si. A questionnaire survey was conducted to obtain information about physical activity in the parks. Binary logistic regression models was used to examine the relationship between the characteristics of physical environments and physical activity in the urban parks. The individual variables, such as mixed land use, ratio of road, sidewalk, pedestrian crossing, intersections density, number of park and park entrance(odds ratio between 1.058 and 2.432, p≤O.1), and network and the shortest distances to park(odds ratio between 0.998 and 0.999, p≤O.Ol) were associated with odds of park-based physical activity. The binary logistic regression model was developed to explain the influence of four factors, accessibility and availability of park, convenience of walking route to park, and abundance of park area derived with factor analysis on park usage for physical activity.

태안해안국립공원 학암포 지역의 조류 현황

강화연(Hwa-Yeon Kang),서슬기(Seul-Gi Seo),박세영(Se-Yeong Park),이지용(Ji-Yong Lee),권영수(Young-Soo Kwon) 국립공원연구원 2015 국립공원연구지 Vol.6 No.4

서해안 육상지역을 통과하는 조류의 이동생태를 파악하기 위해, 2014년 7월부터 2015년 6월까지 태안해안국립공원 학암포 지역의 조류상을 조사하였다. 조사 결과 관찰된 조류는 총 14목 30과 88종 23,230개체이며, 평균 조사일당 159.7개체가 관찰되었다. 전체 우점종은 괭이갈매기 Larus crassirostris 21.1%, 되새 Fringilla montifringilla 18.5%, 박새 Parus major 12.7%, 붉은머리오목눈이 Paradoxornis webbianus 8.2%, 노랑턱멧새 Emberiza elegans 5.8% 순이었다. 이동시기인 5월과 10월에는 각각 56종, 조사일당 평균 310.5개체로 가장 많은 종과 개체수가 기록되었다. 종과 개체수가 가장 적은 시기는 여름철인 8월로, 기록된 종 수는 24종이며 조사일당 평균 95개체가 관찰되었다. 종풍부도(R ) 지수는 가장 많은 종이 관찰된 5월에 가장 높았으나, 종다양도(H )와 종균등도(E )는 오히려 이동시기에 낮은 편으로 나타났으며, 이는 일부 상위 우점종의 개체수가 월등히 증가했기 때문인 것으로 판단된다. 환경부, 문화재청, IUCN 등이 지정한 멸종위기 및 법정보호종은 총 9종이 관찰되었다. From July 2014 to Jun 2015, bird survey was conducted in Hakampo, Taeanhaean National Park. A total of 23,230 and 159.7 per day of birds representing 14 orders, 30 families, 88 species were observed. The most abundant species were the Black-tailed Gull Larus crassirostris (21.1%), the Brambling Fringilla montifringilla (18.5%), the Great Tit Parus major (12.7%), the Vinous-throated Parrotbill Paradoxornis webbianus (8.2%), Yellow-throated Bunting Emberiza elegans (5.8%). The number of species and individual was the highest in May and October, respectively, as 56 species and 310.5 individual per day were recorded. According to Korean Ministry of Environment, Cultural Herigage Adinistration of Korea and IUCN Red List, a total of 9 protected species were recorded during the survey.

Distribution of the Fairy Pitta (Pitta nympha) in the South Korea: A focus on protected areas

Gil-Pyo Hong(홍길표),Jae-Hoon Kim(김재훈),Seul-Gi Seo(서슬기),So-Yeon Cho(조소연),Bo-Yeon Hwang(황보연),Jong-Hyun Park(박종현),Seung-Yeon Lee(이승연),Ga-Young Kim(김가영),Wan-Hee Nam(남완희),Ha-Cheol Sung(성하철) 한국조류학회 2021 한국조류학회지 Vol.28 No.2

국내 팔색조(Pitta nympha) 분포 현황을 알아보고자 전국 국립공원 등 보호지역 중심으로 총 19개 지역을 조사하였고, 전문가 네트워크를 통해 자료를 보강하였다. 전국 19개 지역을 조사한 결과 북한산, 지리산 등 내륙지역 7개 국립공원에서는 팔색조가 확인되지 않았고, 경남 거제, 남해, 해남, 장흥 고흥 등 남해안과 인접한 12개 지역에서 팔색조 서식이 확인되었다. 또한 전문가 네트워크를 통해 수집한 팔색조 출현 위치 정보 82건을 분석한 결과, 전국 8개 광역지자체 모두 팔색조 서식이 확인되었고, 내륙지역(26.8%)보다 해안지역(73.2%)에서 더 많이 출현하였다. 연구 결과를 종합해보면, 우리나라의 팔색조는 주로 제주도와 남해안 지역을 중심으로 많이 분포하며, 그 보다 적은 개체수가 내륙 지역에 넓게 분포하고 있는 것으로 나타났다. The present study investigated the current distribution of Fairy Pitta (Pitta nympha) in South Korea. A total of 19 study areas, including protected areas such as national and provincial parks in South Korea were surveyed, and additional data obtained from an expert network. According to the results, Fairy Pitta was absent in seven inland national parks, including Mt. Bukhan and Chiri parks. Fairy Pitta inhabited 12 areas near the southern coast of South Korea, including Geoje, Namhae, Haenam, Jangheung, Goheung, and Gyeongnam Province. The analysis results of 82 datasets obtained from experts with regard to the locations where Fairy Pitta have been spotted showed that the bird inhabits all the eight metropolitan regions in South Korea, and is more frequently spotted in coastal areas (73.2%) than in inland areas (26.8%). The findings demonstrate that Fairy Pitta is mainly distributed in Jeju Island and the southern coast of South Korea, and is distributed in inland areas at relatively lower numbers.

Reduction of mitochondria derived superoxide by Mito-TEMPO improves the porcine oocyte maturation

Seul-Gi Yang,Hyo-Jin Park,Jin-Woo Kim,Min-Ji Kim,Ho-Guen Jegal,In-Su Kim,Min-Young Guk,Sun-Mi Park,Ji-Eun Lee,Deog-Bon Koo,Joung Jun Park 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

In general, the shape of cumulus-oocyte-complexes (COCs) at germinal vesicle (GV) stage is important roles on meiotic maturation of porcine oocyte during in vitro maturation (IVM). Then, mitochondria produce reactive oxygen species (ROS) such as superoxide from electron transport system during oocyte maturation. ROS levels on oocytes are regulated by various antioxidant enzymes in cumulus cells (CCs). However, the effect of mitochondria derived superoxide production from CCs during IVM of porcine oocyte has not been reported. Firstly, we divided groups according to large number of CCs (Grade 1: G1) and small number of CCs (Grade 2: G2). Then, we counted cumulus cells of G1 and G2 oocyte by using haemocytometer. The oocyte maturation rate was significant decreased (p < 0.05) in G2 oocytes than that of G1 oocytes. We measured mitochondria derived superoxide in G1 and G2 COCs by using Mito-SOX staining. Mitochondrial superoxide was higher in G2 COCs than G1 COCs. Then, the mRNA expression levels of antioxidant enzymes (SOD1, SOD2 and PRDX3) in G2 COCs were decreased compared to G1 COCs. To reduce mitochondria derived superoxide, we used Mito-TEMPO as mitochondrial superoxide scavenger. Oocyte maturation rates in both G1 and G2 groups treated with Mito-TEMPO were increased than that of non-treated groups. Mitochondrial superoxide was lower in G1 and G2 groups treated with Mito-TEMPO than that of non-treatment groups. The mRNA expression levels of antioxidant enzymes in G1 and G2 COCs treated with Mito-TEMPO were increased compared to non-treated groups. Based on these findings, we suggest that reduction of mitochondria derived superoxide by Mito-TEMPO assists maturation competence in porcine oocytes.

Neurodevelopmental Outcomes and Brain Volumetric Analysis of Low-Grade Intraventricular Hemorrhage

Seul Gi Park,Hyo Ju Yang,Soo Yeon Lim,Seh Hyun Kim,Seung Han Shin,Ee Kyung Kim,Han Suk Kim 대한신생아학회 2023 Neonatal medicine Vol.30 No.2

Purpose: Extremely preterm infants are prone to brain injury and underdevelopment. Intraventricular hemorrhage (IVH) is the most common cause of brain injury and a significant risk factor for neurodevelopmental delay in preterm infants. Severe IVH is known to have a poor outcome; however, the outcomes of low-grade IVH remain controversial. This study aimed to evaluate neurodevelopmental outcomes and brain segmental volumes of preterm infants with low-grade IVH. Methods: This retrospective cohort study included 109 extremely preterm infants who underwent term equivalent age-magnetic resonance imaging and neurodevelopmental evaluation at a corrected age of 18 to 24 months. We compared infants with and without low-grade IVH. Results: Among the 109 extremely preterm infants, 25 had low-grade IVH and 84 had no IVH. There were no significant differences in the neurodevelopmental outcomes between the low-grade and no IVH groups. In multivariate analysis, low-grade IVH was associated with a smaller medullary volume (adjusted odds ratio, 0.575; 95% confidence interval, 0.346 to 0.957; P=0.034). Conclusion: We found no significant differences in the neurodevelopmental outcomes of extremely preterm infants at a corrected age of 18 to 24 months between those with low-grade IVH and those without IVH. Low-grade IVH was associated with a smaller medullary volume.

Placental defects in Asb2 knockout mice is associated with heart defects and early embryonic lethality

Seul Gi Park,Eun-kyoung Kim,Hyoung-Chin Kim,Won-Kee Yoon,Ki-Hoan Nam,Sang-Yoon Nam 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Asb2, ankyrin repeat and SOCS box protein 2 form an E3 ubiquitin ligase complex. Asb2 ubiquitin ligase activity drives the degradation of filamins, which have essential functions in humans [1, 2]. The placenta is a temporary organ that is formed during pregnancy, and normal placentation is important for survival and growth of the fetus [3]. Recent studies have shown that approximately 25-30% of knockout (KO) mice have non-viable offspring, and 68% of knockout lines exhibit placental dysmorphologies [4]. There are very few studies on Asb2, with insufficient researches on its role in placental development. We generated Asb2 knockout mice and investigated Asb2 expression during organogenesis, and identified its role in early embryonic and placental development. The external morphology of KO embryos revealed abnormal phenotypes including growth retardation, peri-cardial effusion, pale color, and especially heart beat defect from E 9.5. Furthermore, Asb2 expression was observed in the heart from E 9.5, indicating that it is specifically expressed during early heart formation and defects, result in embryonic lethality. Histological analysis of E 10.5 KO mouse heart showed malformations such as failure of chamber formation, reduction in trabeculated myocardium length, absence of mesenchymal cells, and destruction of myocardial wall. Moreover, the histological observation of the placenta for Asb2-deficient mice showed abnormal phenotypes including small labyrinth and reduced vascular complexity, indicating that failure to establish mature circulatory pattern affects the embryonic development and associated with the early mortality. Collectively, our results demonstrate that Asb2 knockout mice have placental defects, and failure to form a normal cardiac septum, and thereby result in embryonic lethality in utero at around E 9.5. References [1] Babon, J.J., et al., The SOCS box domain of SOCS3: structure and interaction with the elonginBC-cullin5 ubiquitin ligase. J Mol Biol, 2008. 381(4): p. 928-40.[2] Heuze, M.L., et al., ASB2 is an Elongin BC-interacting protein that can assemble with Cullin 5 and Rbx1 to reconstitute an E3 ubiquitin ligase complex. J Biol Chem, 2005. 280(7): p. 5468-74.[3] Cross, J.C., D.G. Simmons, and E.D. Watson, Chorioallantoic morphogenesis and formation of the placental villous tree. Ann N Y Acad Sci, 2003. 995: p. 84-93.[4] Rossant, J. and J.C. Cross, Placental development: lessons from mouse mutants. Nat Rev Genet, 2001. 2(7): p. 538-48.

Changes in Mitochondria Functions and Dynamics According to the Lipid Density of Zygote in Porcine Embryo

Seul-Gi Yang,Hyo-Jin Park,Jin-Woo Kim,Jae-Young Park,Jae-Min Jung,Min-Ji Kim,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

Changes in Mitochondria Functions and Dynamics According to the Lipid Density of Zygote in Porcine Embryo

Seul-Gi Yang,Hyo-Jin Park,Jin-Woo Kim,Jae-Young Park,Jae-Min Jung,Min-Ji Kim,Deog-Bon Koo 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

Mitochondria are well known to regulate the mammalian embryo development. Recent studies showed that the mitochondrial dynamics responses are mainly generated through mitochondrial membrane potential (MMP) and cellular ATP production, which is dependent on mitochondrial reactive oxygen species (ROS). However, these mechanisms are unclear on development process of preimplantation porcine embryos. The aim of this study was to evaluate that difference of the mitochondrial dynamics-derived various functions on the embryo development according to lipid composition of zygote. First, zygote was classify two groups (high lipid, grade 1: G1 and low lipid, grade 2: G2) by lipid composition of cytoplasm. And, we performed the in vitro culture (IVC) using zygote of divided groups. The nuclei numbers and developmental rates of blastocysts were lower in G2 than those of G1 embryos. Next, we investigated the intracellular ROS and mitochondrial derived superoxide production in porcine embryos by using DCF-DA and Mito-SOX staining. As expected, both intracellular ROS and mitochondrial derived superoxide were significantly increased (p<0.05) in the preimplantation stage embryos of G2 group compared with G1 group. In addition, to observe difference of the mitochondrial functions, we investigated the mitochondrial membrane potential (MMP, ΔΨ) and contents of ATP in the preimplantation stage embryos by using JC-1 kit and ATP determination kit. These functions of mitochondria were dramatically reduced in cleavage stage embryos or blastocysts of G2 group. Finally, to verify the difference of the mitochondrial dynamics-derived various functions, we investigated the expressions of mitochondrial fission (Drp1, pDrp1-616) and fusion (Mfn1, Mfn2) factors by Western blotting analysis. Interestingly, the protein levels of pDrp1-616 in embryos of G1 group were continuously increased until blastocyst stage. Whereas, the expression patterns of Mfn1/2 in embryos of G2 group were significantly reduced during IVC progression. The expression patterns of mitochondria dynamic between the two groups were shown opposite. These results demonstrated that the lipid contents of zygote were related the positive-correlation with mitochondrial dynamics-derived functions in porcine embryos. Moreover, we suggest that lipid of zygote is play a important role on mitochondrial functions and dynamics during preimplantation embryos development in pigs.

Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (<i>Oryza sativa </i>L.)

Park, Seul Gi,Ubaidillah, Mohammad,Kim, Kyung-Min 'Scientific Research Publishing, Inc.' 2013 American journal of plant sciences Vol.4 No.11

Evaluation of Barley and Wheat Germplasm for Resistance to Head Blight and Mycotoxin Production by Fusarium asiaticum and F. graminearum

Seul Gi Baek(Seul Gi Baek),Jin Ju Park(Jin Ju Park),Sosoo Kim(Sosoo Kim),Mi-Jeong Lee(Mi-Jeong Lee),Ji-Seon Paek(Ji-Seon Paek),Jangnam Choi(Jangnam Choi),Ja Yeong Jang(Ja Yeong Jang),Jeomsoon Kim(Jeom 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Fusarium head blight (FHB) is one of the most serious diseases in barley and wheat, as it is usually accompanied by the production of harmful mycotoxins in the grains. To identify FHB-resistant breeding resources, we evaluated 60 elite germplasm accessions of barley (24) and wheat (36) for FHB and mycotoxin accumulation. Assessments were performed in a greenhouse and five heads per accession were inoculated with both Fusarium asiaticum (Fa73, nivalenol producer) and F. graminearum (Fg39, deoxynivalenol producer) strains. While the accessions varied in disease severity and mycotoxin production, four wheat and one barley showed <20% FHB severity repeatedly by both strains. Mycotoxin levels in these accessions ranged up to 3.9 mg/ kg. FHB severity was generally higher in barley than in wheat, and Fa73 was more aggressive in both crops than Fg39. Fg39 itself, however, was more aggressive toward wheat and produced more mycotoxin in wheat than in barley. FHB severity by Fa73 and Fg39 were moderately correlated in both crops (r = 0.57/0.60 in barley and 0.42/0.58 in wheat). FHB severity and toxin production were also correlated in both crops, with a stronger correlation for Fa73 (r = 0.42/0.82 in barley, 0.70 in wheat) than for Fg39.